Article
Nutrition & Dietetics
Agata Walkowiak-Brodka, Natalia Piekus-Slomka, Kacper Wnuk, Bogumila Kupcewicz
Summary: Diabetes mellitus is a growing metabolic disease, and there is a need to find natural products for preventative and early-stage treatment. White mulberry leaves, known for their beneficial properties, can regulate postprandial glucose concentration. However, the quality of dietary supplements containing white mulberry is found to be low, lacking the expected health benefits. A proposed alternative method for assessing the quality of these products involves determining DNJ content using ATR-FTIR spectroscopy combined with PLS regression.
Article
Plant Sciences
Lucia Marchetti, Alessio Saviane, Antonella dalla Monta, Graziella Paglia, Federica Pellati, Stefania Benvenuti, Davide Bertelli, Silvia Cappellozza
Summary: Recent study on 1-DNJ in Morus sp.pl. leaves in Italian crops showed similar levels to Asian cultivations, indicating a potential impact of climate and genotypes on the compound's presence. The research highlights the importance of exploring agronomic practices and cultivar selection to standardize 1-DNJ levels in leaves.
Article
Biochemistry & Molecular Biology
Soo Takasu, Isabella Supardi Parida, Yoshihiro Kojima, Toshiyuki Kimura, Kiyotaka Nakagawa
Summary: Studies have shown that converting GAL-DNJ to DNJ may enhance the therapeutic potency of mulberry leaves, leading to the proposal of an enzymatic-based method. This novel approach aims to improve the bioavailability and effectiveness of mulberry leaves through the hydrolysis of GAL-DNJ to DNJ.
Article
Food Science & Technology
Zhenjiang Wang, Fanwei Dai, Cuiming Tang, Gengsheng Xiao, Zhiyi Li, Guoqing Luo
Summary: The study found that DNJ is mainly present in the young tissues of mulberry fruit, and the concentration decreases as the fruit matures. Among the 146 varieties, the variety has a significant impact on the DNJ concentration of mulberry fruit, while the plant origin does not have a significant effect.
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
(2021)
Article
Plant Sciences
Jingqiong Wan, Yangzhen Liao, Jia Liu, Wenmin Du, Chang Liu, Yuan Wei, Zhen Ouyang
Summary: The novel C-methyltransferase MaMT1 was found to catalyze the conversion of piperidine to 2-methylpiperidine in mulberry leaves, potentially playing a role in the methylation step of DNJ biosynthesis. This discovery provides new insights into the research of DNJ alkaloid biosynthesis and the C-methyltransferase family.
Article
Medicine, Legal
Tilo D. Schachel, Rasmus Schulte-Ladbeck
Summary: This study aims to address the analytical challenge of determining nitrocellulose in smokeless powders. By using Direct Analysis in Real Time Mass Spectrometry, the presence of nitrocellulose in smokeless powders can be rapidly and accurately detected, bypassing the issues associated with traditional detection methods.
FORENSIC SCIENCE INTERNATIONAL
(2022)
Article
Chemistry, Analytical
Xuewei Lin, Hao Wu, Gefei Huang, Qian Wu, Zhong-Ping Yao
Summary: This study developed a simple, rapid, and high-throughput approach for the authentication of red wine by combining matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and direct analysis in real time mass spectrometry (DART-MS). The approach, coupled with orthogonal partial least squares discrimination analysis (OPLS-DA), successfully classified 535 wines from 8 countries with high accuracy rates. This study demonstrated the feasibility of combining multiple mass spectrometric techniques for wine analysis.
ANALYTICA CHIMICA ACTA
(2023)
Article
Nutrition & Dietetics
Kamonpan Fongsodsri, Thanchanit Thaipitakwong, Kitiya Rujimongkon, Tapanee Kanjanapruthipong, Sumate Ampawong, Onrapak Reamtong, Pornanong Aramwit
Summary: A study found that mulberry leaf powder can improve insulin resistance and inflammation, and promote mitochondrial function in prediabetic and early-stage type 2 diabetes patients.
Article
Agricultural Engineering
Ping-Yue Wang, Fei-Fan Shuang, Jia-Xin Yang, Yu-Xiong Jv, Run-Ze Hu, Tao Chen, Xiao-Hui Yao, Wei-Guo Zhao, Li Liu, Dong-Yang Zhang
Summary: In this study, a method combining microwave-assisted extraction and hydrolysis (MAEHM) with automatic amino acid analyzer (AAA) was developed and applied to extract and determine amino acids from mulberry leaves. The MAEHM-AAA method showed superior extraction efficiency and faster reaction rate compared to conventional methods. The method significantly reduced the preprocessing time and demonstrated great sensitivity with low limits of detection. The accuracy, precision, and stability of the method were also validated. The MAEHM-AAA method was successfully applied to quantify 17 target compounds in mulberry leaves.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Food Science & Technology
A. Lestido-Cardama, R. Paseiro-Cerrato, L. K. Ackerman, R. Sendon, A. Rodriguez-Bernaldo De Quiros
Summary: The study investigated potential contamination with brominated flame retardants in food contact articles purchased in Spain using a combination of different techniques to identify contaminated samples and determine the migration of flame retardants.
FOOD PACKAGING AND SHELF LIFE
(2022)
Article
Food Science & Technology
A. Lestido-Cardama, R. Paseiro-Cerrato, L. K. Ackerman, R. Sendon, A. Rodriguez-Bernaldo de Quiros
Summary: This study investigated the potential contamination of brominated flame retardants (BFRs) in several food contact articles (FCAs) purchased in Spain using a combination of DART-HRMS, XRF, and HPLC-MS / MS. The results showed that DART and XRF techniques could effectively screen potential contaminated samples, and the HPLC-MS/MS method was suitable for the determination of TBP, TBBPA, and BDE-209.
FOOD PACKAGING AND SHELF LIFE
(2022)
Article
Biochemical Research Methods
Fubang Liu, Ying Zhang, Jifen Wang, Jiahua Ji
Summary: A rapid and accurate method for identifying benzodiazepines and related metabolites was developed and validated in this study. Direct analysis in real-time tandem mass spectrometry was used for sample analysis, and liquid-liquid extraction was employed for sample preparation. The method showed good linearity and recovery, enabling efficient and accurate qualification and quantification of case specimens.
DRUG TESTING AND ANALYSIS
(2023)
Article
Biochemical Research Methods
Alexander Ecke, Tanja Westphalen, Jane Hornung, Michael Voetz, Rudolf J. Schneider
Summary: Increasing contamination of environmental waters with pharmaceuticals requires fast and reliable analytical methods. The magnetic bead-based immunoassay reported here for the determination of diclofenac in water samples offers a significantly lower limit of detection than traditional ELISA, with shorter operating time and simplicity.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Salem Hayar, Rawan Zeitoun, Britt Marianna Maestroni
Summary: The QuEChERS method was used to detect pesticide residues in grape leaves, with validation for 33 pesticides representing 17 chemical groups commonly used in grape production. Analysis of 17 market brands in Lebanon showed dried vine leaves to be more contaminated than brine-preserved or stuffed vine leaves, with systemic fungicides being the most frequently detected pesticide among all compounds tested.
Review
Pharmacology & Pharmacy
Sikai Chen, Miaomiao Xi, Feng Gao, Min Li, TaiWei Dong, Zhixin Geng, Chunyu Liu, Fengyu Huang, Jing Wang, Xingyu Li, Peifeng Wei, Feng Miao
Summary: The effectiveness of herbal medicine in treating diabetes has grown, but the precise mechanism is still unclear. Mulberry leaf, a commonly used herb in traditional Chinese medicine, has been found to have strong anti-diabetic properties. The bioactive compounds in mulberry leaves, such as flavonoids and polysaccharides, play a crucial role in regulating blood glucose levels and improving insulin resistance.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)