Article
Toxicology
Jochem Louisse, Patrick P. J. Mulder, Arjen Gerssen, Geert Stoopen, Deborah Rijkers, Milou G. M. van de Schans, Ad A. C. M. Peijnenburg
Summary: “The study utilized a bioassay-directed analysis approach to identify potentially toxic PAs not yet included in monitoring programs. The findings suggest that this method is effective in identifying candidate PAs with genotoxic activity.”
ARCHIVES OF TOXICOLOGY
(2022)
Article
Chemistry, Analytical
Yilei Fan, Xingsen Zong, Jiwu Liu, Xing Ke, Zhongping Huang, Yu Xu
Summary: The study investigates the MS fragmentation pattern of 27 synthetic cannabinoids by electrospray ionization mass spectrometry, revealing the characteristic fragmentation pathway in the positive mode of electrospray ionization. This fragmentation pattern is applied to quickly screen whether electronic cigarette oil and tobacco from drug cases contain synthetic cannabinoids, providing new evidence for rapid structure identification.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Analytical
Moolchand Kurmi, Vipul Suryavanshi, Narayana Swamy Panduranga, Karthik Jayaraman, Lakshmikant Bajpai, William Fish, Yue Hu, Hemant Bhutani
Summary: The study presents a liquid chromatographic method for quantifying DMPE-PEG 2000, an important component of lipid-based nanoparticles, and investigates its degradation products. The method was validated for precision, linearity, sensitivity, solution stability and robustness, demonstrating good intra-day precision, inter-day precision, sensitivity, and linearity in the range from 210 μg/mL to 390 μg/mL. The stability of DMPE-PEG 2000 under different stress and storage conditions was also evaluated, with degradation products identified and a tentative pathway proposed.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Analytical
Ke Sherry Li, John G. Quinn, Matthew J. Saabye, Jesus F. Salcido Guerrero, Jim Nonomiya, Qihui Lian, Wilson Phung, Yevgeniy Izrayelit, Benjamin T. Walters, Amy Gustafson, Nicholas F. Endres, Maureen H. Beresini, Melinda M. Mulvihill
Summary: With recent advances in drug discovery, targeted covalent inhibitors have gained attention for their potential in treating acute and chronic diseases. This study presents a mass spectrometry-based platform for rapid kinetic analysis of irreversible covalent inhibitors. The platform allows for high-throughput characterization of covalent inhibitors through automated sample preparation and rapid MS analysis. A bimolecular reaction model is applied to extract important data, providing flexibility in studying covalent inhibitors with different properties.
ANALYTICAL CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Xunyuan Jiang, Lu Xiao, Yan Chen, Congling Huang, Jiale Wang, Xuemei Tang, Kai Wan, Hanhong Xu
Summary: In this study, the degradation kinetics, pathways, and aquatic toxicity of the new fused heterocyclic insecticide pyraquinil in water were investigated for the first time. The results showed that pyraquinil is easily degradable in natural water, with faster degradation in alkaline conditions and higher temperatures. Fifteen transformation products of pyraquinil were identified, and their toxicities were found to be lower than that of pyraquinil except for TP484, which posed a higher toxicity. These findings are important for understanding the fate and environmental risks of pyraquinil and guiding its scientific and reasonable use.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Mahmoud A. Tantawy, Dalia A. Elshabasy, Nadia F. Youssef, Sawsan M. Amer
Summary: This paper presents a novel potentiometric approach using two sensors, one ionophore-free and one ionophore-doped, for the determination of palonosetron HCl. The ionophore-doped sensor exhibited a lower detection limit, enhanced selectivity, and faster response compared to the ionophore-free sensor. Both sensors showed stable response over a pH range of 3.0-8.0 and successfully determined the drug in both presence of its oxidative degradation products and in commercially available parenteral solution without interference from other additives.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Annabel Mehl, Wolfgang Schwack, Gertrud E. Morlock
Summary: The autoTLC-LC-MS system, utilizing an on-surface autosampler, enables the hyphenation of liquid chromatography-mass spectrometry, combining normal phase high-performance thin-layer chromatography with reversed phase high-performance liquid chromatography and high-resolution mass spectrometry. Through verification using a multi-class antibiotic mixture, the system demonstrated high precision and reproducibility in analyte detection.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Environmental Sciences
Astrid Spielmeyer, Christopher R. Loeffler, Oliver Kappenstein
Summary: This study conducted an in-depth investigation using a semi-targeted LC-MS/MS approach to analyze tissue samples of Lutjanus bohar from the Indian Ocean and Pacific Ocean. The samples revealed a complex CTX contaminant profile, with the presence of several congeners of the CTX3C-group, which have a strong association with ciguatera poisoning. Furthermore, there were no distinguishable differences in toxin profile among individual samples or among the different oceanic capture regions, suggesting either a species-specific CTX metabolism or the emergence of an interoceanic CTX toxin profile.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biochemical Research Methods
Bhoopendra Singh Kushwah, Mohit M. Thummar, Amrej Singh Yadav, Vivek Dhiman, Gananadhamu Samanthula
Summary: This study describes the forced degradation of benidipine and the separation and characterization of its degradation products using HPLC and LC-MS/MS. The developed method can be used for quality control of drugs in laboratories.
BIOMEDICAL CHROMATOGRAPHY
(2023)
Article
Chemistry, Analytical
Doaa M. Mustafa, Nancy Magdy, Noha F. El Azab
Summary: The study focused on the development of a stability-indicating HPLC-DAD technique for Vericiguat (VER) analysis. VER was found to be sensitive to alkaline, oxidative and thermal degradation. The analysis method was validated to be specific, fast, simple, precise and accurate for routine analysis and quality control of VER in its pharmaceutical formulation. The technique was also used to investigate the kinetics of alkaline, oxidative and dry heat degradation.
Article
Biochemistry & Molecular Biology
Ibrahim Aissa, Aniko Kilar, Agnes Doernyei
Summary: This study investigated the fragmentation behavior of synthetic lipid A derivatives using mass spectrometry, proposing new dissociation pathways and interpreting fragment ions based on possible mechanisms. The findings contribute to a better understanding of lipid A fragmentation processes and the differentiation of isomers with single ester-linked fatty acids at different positions.
Article
Biochemistry & Molecular Biology
Awadh M. Ali, Mohammed M. Alanazi, Mohamed W. Attwa, Hany W. Darwish
Summary: In this study, a rapid and specific separation method for Infigratinib and its degradation products was developed using the Quality by Design principle. The reversed-phase high-performance liquid chromatography technique was utilized for chromatographic separation, while UPLC-quadrupole tandem mass spectrometry was employed for structural analysis and characterization of the degradation products. The method was verified and various parameters were evaluated. Additionally, the toxicity and mutagenicity of these compounds were predicted using in silico software.
Article
Biochemical Research Methods
Prasad Phapale, Andrew Palmer, Rose Muthoni Gathungu, Dipali Kale, Britta Brugger, Theodore Alexandrov
Summary: EMBL-MCF is an open LC-MS/MS spectral library with over 1600 fragmentation spectra from 435 authentic standards of endogenous metabolites and lipids. It features chromatographic profiles acquired with different LC-MS methods and coverage of different adduct ions. The library contains many biologically important metabolites and unique metabolites and lipids compared to other public libraries.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Anatolii Spesyvyi, Jan Zabka, Miroslav Polasek, Ales Charvat, Jürgen Schmidt, Frank Postberg, Bernd Abel
Summary: The article introduces a device for generating charged ice particles in vacuum, produced by electrospraying ionized water at atmospheric pressure. The particles undergo evaporative cooling when transferred to vacuum and are filtered by two consecutive quadrupole mass filters. Velocity and charge of the particles are measured by a nondestructive image charge detector. This device allows the preparation of single particle beams with various size distributions and kinetic energies.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Biochemistry & Molecular Biology
Jin Young Lee, Kyoung Chan Lim, Hyun Suk Kim
Summary: In this study, the degradation products and fragmentation pathways of Novichok (A234) in aqueous solution at different pH levels were investigated using liquid chromatography-tandem mass spectrometry. The results showed that A234 had the fastest hydrolysis rate under acidic conditions, with the same degradation products formed at all pH levels.
Article
Pharmacology & Pharmacy
Sarah El Mershati, Agathe Thouvenin, Philippe-Henri Secretan, Pascale De Lonlay, Caroline Tuchmann-Durand, Salvatore Cisternino, Joel Schlatter
Summary: Hydroxychloroquine is an antimalarial drug that is also used to treat conditions like rheumatoid arthritis and lupus. While it has been proposed for COVID-19 therapy, it is currently only available in tablet form, which may not be suitable for certain patients.
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Celia Djayet, Dominique Bremond-Gignac, Justine Touchard, Philippe-Henri Secretan, Fabrice Vidal, Matthieu P. Robert, Alejandra Daruich, Salvatore Cisternino, Joel Schlatter
Summary: Congenital aniridia is a rare and severe panocular disease caused by PAX6 haploinsufficiency. Ataluren eye drops aim to restore ocular surface PAX6 haploinsufficiency in aniridia-related keratopathy (ARK) by using an oily formulation containing co-solvents to improve stability. The study demonstrates the physicochemical and microbiological stability of ataluren 1% eye drop formulation in an LDPE bottle with an innovative insert.
Article
Chemistry, Analytical
Philippe-Henri Secretan, Hassane Sadou Yaye, Audrey Sogaldi, Marie Antignac, Lionel Tortolano, Olivier Thirion, Victoire Vieillard, Najet Yagoubi, Bernard Do
Summary: Umifenovir is an antiviral drug approved for influenza treatment in China and Russia. It may be used in other viral contexts, but data on its stability is currently unavailable.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Analytical
Philippe-Henri Secretan, Joel Schlatter, Salvatore Cisternino, Marie Antignac, Olivier Thirion, Hassane Sadou Yaye, Xavier Jouven, Najet Yagoubi, Bernard Do
Summary: Ruxolitinib, a Janus Kinase inhibitor, is approved for myelofibrosis treatment and shows potential for skin and infectious disease treatment. Despite being light-sensitive, there is a lack of data on its photodegradation processes. Studies suggest that photo-oxidation mechanisms may lead to structural changes and increased mutagenicity in certain degradation products.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Pharmacology & Pharmacy
P-H Secretan, O. Thirion, V. Vieillard, J. Saunier, K. Razazi, M. Paul, B. Do
Summary: The potential usefulness of lopinavir-ritonavir in the treatment of Covid 19 led to a national shortage of Kaletra(R) syrup in France. As a result, healthcare providers in the intensive care units of Parisian hospitals had to crush lopinavir-ritonavir tablets and mix the powder with milk for administration via nasogastric tube. However, the poor control and difficulties of this method prompted the development of a liquid formulation as an alternative to the shortage of Kaletra(R) oral solution.
ANNALES PHARMACEUTIQUES FRANCAISES
(2022)
Article
Biochemistry & Molecular Biology
David Nguyen, Philippe-Henri Secretan, Camille Cotteret, Emmanuelle Jacques-Gustave, Celine Greco, Christine Bodemer, Joel Schlatter, Salvatore Cisternino
Summary: Recent studies have shown the potential benefit of repurposing oral erlotinib treatment for rare skin diseases such as Olmsted syndrome. Developing a topical erlotinib cream instead of using oral tablets can be more effective in treating skin disorders and reducing systemic side effects. The study also found that using dimethyl sulfoxide, neutral pH, and an antioxidant-containing topical cream were important factors in maintaining erlotinib concentration and stability.
Article
Pharmacology & Pharmacy
Guillaume Le Guyader, Bernard Do, Ivo B. Rietveld, Pascale Coric, Serge Bouaziz, Jean-Michel Guigner, Philippe-Henri Secretan, Karine Andrieux, Muriel Paul
Summary: The study finds that polymeric micelles improve the bioavailability of rapamycin, with TPGS significantly enhancing solubility and stability, while poloxamer has a more significant influence on skin bioavailability. The formulation of a hydrogel containing 0.1% rapamycin shows stability for up to 3 months and better biodistribution on human skin compared to hydroalcoholic gel formulations.
Article
Pharmacology & Pharmacy
Maria Gloria Tarantini, Stephanie Ramos, Philippe-Henri Secretan, Laura Guichard, Lamia Hassani, Agnes Bellanger, Julien Mayaux, Patrick Tilleul, Fadwa El Kouari, Hassane Sadou Yaye
Summary: The study demonstrated the physicochemical stability of different combinations of five selected drugs for at least 3 to 7 days at 25 degrees Celsius, providing the potential of minimizing individual doses and reducing unwanted side effects by combining multiple drugs in one syringe.
Article
Pharmacology & Pharmacy
Philippe-Henri Secretan, Maxime Annereau, Willy Kini-Matondo, Bastien Prost, Jade Prudhomme, Lina Bournane, Muriel Paul, Najet Yagoubi, Hassane Sadou-Yaye, Bernard Do
Summary: Nirmatrelvir is an antiviral drug used for the treatment of COVID-19. Understanding its stability and degradation properties is important for potential pharmaceutical development.
Article
Pharmacology & Pharmacy
Tahar Sif eddine Bouchema, Maxime Annereau, Victoire Vieillard, Raphael Boquet, Gisele Abreu Coelho, Florence Castelli, Audrey Solgadi, Muriel Paul, Najet Yagoubi, Philippe-Henri Secretan, Bernard Do
Summary: Selumetinib, an orally administered drug for treating neurofibromatosis, requires development of liquid formulations for dosage adjustment. The study found that selumetinib is sensitive to oxidation and photodegradation in solution, leading to the formation of amide and ester derivatives. Investigating these degradation mechanisms allows for targeted stabilization of the active molecule.
Article
Biochemistry & Molecular Biology
Philippe-Henri Secretan, Victoire Vieillard, Olivier Thirion, Maxime Annereau, Hassane Sadou Yaye, Alain Astier, Muriel Paul, Thibaud Damy, Bernard Do
Summary: In vitro studies have shown that EGCG can prevent the formation of amyloid plaques and induce their clearance. However, its high chemical reactivity poses a challenge for the development of pharmaceutical forms. By forming a deep eutectic with choline chloride, liquid-filled capsules containing soluble EGCG were formulated with improved stability. Preclinical studies showed increased bioavailability compared to marketed capsules, making it a promising development in treating cardiac amyloidosis.
Article
Chemistry, Analytical
Maxime Annereau, Marina Vignes, Tahar Sif Eddine Bouchema, Lucas Denis, Audrey Solgadi, Victoire Vieillard, Muriel Paul, Andre Rieutord, Jacques Grill, Philippe-Henri Secretan, Bernard Do
Summary: ONC201 is a new drug substance used to treat glioblastoma patients in a compassionate manner. To ensure quality and safety during the manufacturing process, the stability profile of ONC201 needed to be determined, including its sensitivity to stressors and potential impurities formed during degradation. Oxidation and light irradiation were found to cause the formation of 20 degradation products. The structures of these products were identified through liquid chromatography-high-resolution mass spectrometry and comparison with ONC201's protonated ion.
Review
Medicine, General & Internal
Gisele Abreu Coelho, Philippe-Henri Secretan, Lionel Tortolano, Loic Charvet, Najet Yagoubi
Summary: This review aims to identify and discuss the different stages of chronic wound progression, focusing on the changes in the biochemical composition of the wound microenvironment, particularly in venous leg ulcers (VLUs). It provides detailed information about the pathophysiology of venous ulcers and reviews the known changes in the VLU microenvironment as the VLU evolves. Additionally, it discusses simulating conditions for VLU and highlights potentially relevant simulating media to assess the biological safety of medical devices intended to be in contact with these wounds.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Maxime Annereau, Marina Vignes, Lucas Denis, Andre Rieutord, Francois-Xavier Legrand, Francois Rioblanc, Muriel Paul, Jacques Grill, Philippe-Henri Secretan, Bernard Do
Summary: This study aimed to improve the understanding of the degradation pathways of ONC201 using data from ab initio calculations and experimental work. The reactivity of ONC201 to light and oxidation conditions was found to be highly dependent on pH. This information is crucial for better understanding the stability results in the presence of antioxidant agents and determining the right conditions for them to act.
Article
Pharmacology & Pharmacy
Sephora Belo, Justine Touchard, Philippe-Henri Secretan, Fabrice Vidal, Vincent Boudy, Salvatore Cisternino, Joel Schlatter
Summary: A rectal pentobarbital gel was formulated and evaluated for stability, showing chemical stability for at least 90 days and effective sedation for children.
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
