Article
Fisheries
Lin Su, Huimin Guo, Baoshan Guo, Jingyuan Yi, Zongrui Yang, Shun Zhou, Yunji Xiu
Summary: In recent years, combination vaccines have become necessary to prevent fish diseases caused by multiple infections simultaneously. In this study, an inactivated bivalent vaccine was prepared for Aeromonas salmonicida and Edwardsiella tarda. After vaccination, the vaccinated turbot showed a relative percentage survival (RPS) of 77.1% when challenged with A. salmonicida and E. tarda. The study also found that the vaccinated group had upregulated serum antibody titer and lysozyme activity, as well as increased expression levels of genes related to antigen recognition, processing, and presentation.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Emmanuelle Moreau, Lionel Pineau, Florine Bachelet, Antoine Rostang, Kenny Oberle, Segolene Calvez
Summary: This study aimed to develop a method of infectious challenge in large Rainbow trout by immersion, simulating natural infection conditions. The results showed that a 24-hour contact period led to infection in all fish with a mortality rate of 53.25%. The infected fish developed acute infection symptoms and lesions similar to furunculosis, and antibodies against the bacterium were produced 4 weeks after challenging compared to the non-challenged group.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Fei Sun, Jie Zhou, Yuanxing Zhang, Qin Liu, Qiyao Wang, Xiaohong Liu
Summary: An inactivated vaccine containing a compound adjuvant of ginseng stem leaf saponins and aluminum hydroxide gel (GSLS/Alum) was developed against Aeromonas salmonicida in turbot farming. The vaccine demonstrated a significant increase in serum antibody titer and a high relative percentage survival (RPS) of turbot. The formulation of GSLS and Alum proved to be a potential compound adjuvant in turbot vaccine development, showing excellent protection against natural infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Yunji Xiu, Baoshan Guo, Jingyuan Yi, Huimin Guo, Hetron Mweemba Munang'andu, Cheng Xu, Shun Zhou
Summary: This study used RNA-seq to analyze the transcriptomic profile of kidney tissue in turbot after immunization with an inactivated bivalent vaccine against Aeromonas salmonicida and Edwardsiella tarda. The results revealed that the vaccine induced upregulation and downregulation of immune-related genes, including cytokines, complement system, and chemokines, enhancing the immune response against these pathogens in turbot.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Immunology
Yingrui Li, Lin Su, Xiaofei Liu, Huimin Guo, Shun Zhou, Yunji Xiu
Summary: In this study, differentially methylated regions (DMRs) were identified using Whole Genome Bisulfite Sequencing (WGBS), and significantly differentially expressed genes (DEGs) were identified through Transcriptome sequencing. The DNA methylation state of the gene promoter region affected gene transcriptional activity, which was validated by double luciferase report assay and DNA pull-down assay. The study revealed the role of DNA methylation in the immune mechanism of turbot after immunization with inactivated vaccine of Aeromonas salmonicida.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Fisheries
Ting Xue, Yiping Liu, Min Cao, Jie Li, Mengyu Tian, Lu Zhang, Beibei Wang, Xiaoli Liu, Chao Li
Summary: This study investigated the regulatory roles of circRNA and miRNA in the early immune response of turbot following injection of an inactivated Aeromonas salmonicida vaccine. Through high-throughput sequencing, differentially expressed circRNAs, miRNAs, and mRNAs were identified, suggesting a complex multi-molecule regulatory network involving immune-related genes. Further research is needed to fully understand the regulatory mechanisms of these circRNA-miRNA-mRNA networks in teleost immune responses.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Yongwei Yan, Yaokuan Liu, Zhaolan Mo, Jie Li, Shulan Liu, Ye Gao, Guiyang Li, Jie Li
Summary: A developed A. salmonicida subsp. masoucida inactivated vaccine for turbot has been proven to be effective in protecting the fish for at least 24 weeks under both laboratory and field conditions, with the emulsified vaccine showing better results than the water based vaccine. The vaccine not only reduces the infection probability by other pathogens or parasites, but also promotes fish growth without severe side-effects.
Article
Immunology
A. Rodriguez-Pedrouzo, J. Cisneros-Sureda, D. Martinez-Matamoros, D. Rey-Varela, M. Balado, J. Rodriguez, M. L. Lemos, M. Folgueira, C. Jimenez
Summary: Zebrafish is an excellent model for studying bacterial infections in fish. This study used zebrafish as a model to investigate the infection process of Aeromonas salmonicida in fish and evaluated the use of fluorescence microscopy to track the infection in vivo.
MICROBIAL PATHOGENESIS
(2023)
Article
Fisheries
Athina Papadopoulou, Sean J. Monaghan, Nicola Bagwell, Mickael Teixeira Alves, David Verner-Jeffreys, Tim Wallis, Andrew Davie, Alexandra Adams, Herve Migaud
Summary: This study provides important new information on the onset of adaptive immunity in ballan wrasse and highlights that immersion vaccination for protection against aAs should be performed at later developmental stages in the hatchery.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Environmental Sciences
Marta Libran-Perez, Patricia Pereiro, Antonio Figueras, Beatriz Novoa
Summary: We conducted RNA-Seq analysis to investigate the response of turbot to infection by Aeromonas salmonicida. We found that infection led to the modulation of immune-related and metabolism-related genes, with one of the most upregulated genes being the hormone leptin. Leptin altered the immune and metabolic response to the bacteria, resulting in increased survival rate without affecting bacterial load. This highlights the complex interplay between bacterial activity and food intake, metabolism, and inflammation.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Fisheries
Yunji Xiu, Lin Su, Baoshan Guo, Jingyuan Yi, Zongrui Yang, Shun Zhou
Summary: In this study, RNA-seq technology was used to analyze the head kidney transcriptome of turbot vaccinated with a bivalent inactivated vaccine against Aeromonas salmonicida and Vibrio scophthalmi. The results showed that several immune-related genes were up-regulated after vaccination, providing insights into the immune response mechanisms of turbot to vaccination.
Article
Biology
Ting Xue, Yiping Liu, Min Cao, Mengyu Tian, Lu Zhang, Beibei Wang, Xiaoli Liu, Chao Li
Summary: In this study, the expression of circRNAs, miRNAs, and mRNA in the turbot spleen infected with Aeromonas salmonicida was analyzed by high-throughput sequencing. Results indicated that circRNAs and miRNAs may regulate immune-related genes through the circRNA-miRNA-mRNA network and participate in the immune response. This study highlights the potential role of non-coding RNAs in regulating immune processes in teleosts.
Article
Biotechnology & Applied Microbiology
Zhenhe Xu, Peng Jin, Xiangshan Zhou, Yuanxing Zhang, Qiyao Wang, Xiaohong Liu, Shuai Shao, Qin Liu
Summary: The study demonstrated that the newly isolated A. salmonicida subsp. masoucida phage vB_AsM_ZHF exhibited potent antibacterial effect against the pathogen both in vitro and in vivo, reducing bacterial burden and inflammatory response in infected turbot. This phage showed promising potential as an alternative therapeutic agent to antibiotics for controlling multidrug-resistant A. salmonicida subsp. masoucida infections in the aquaculture industry.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Fisheries
Yolanda Torres-Corral, Oscar Gonzalez-Barreiro, Ana Riaza, Ysabel Santos
Summary: This study identified the possible routes of infection of Aeromonas salmonicida subsp. achromogenes in turbot and sole, and confirmed its role as the causative agent of mortality. Further research is needed to understand the route of entry and dissemination of this bacterium in fish, which will help improve treatment and preventive strategies.
Article
Multidisciplinary Sciences
Omri Tau, Alice Henley, Anton N. Boichenko, Nadezhda N. Kleshchina, River Riley, Bingxing Wang, Danielle Winning, Ross Lewin, Ivan P. Parkin, John M. Ward, Helen C. Hailes, Anastasia Bochenkova, Helen H. Fielding
Summary: This study determines the electron detachment energy of the green fluorescent protein chromophore in aqueous solution using UV liquid-microjet photoelectron spectroscopy and quantum chemistry calculations. The research highlights the importance of electronically excited states of the chromophore in photo-induced electron transfer processes in the condensed phase.
NATURE COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
L. Rivera, M. A. Lopez-Patino, D. L. Milton, T. P. Nieto, R. Farto
JOURNAL OF APPLIED MICROBIOLOGY
(2015)
Article
Fisheries
E. P. Lago, T. P. Nieto, R. Farto
DISEASES OF AQUATIC ORGANISMS
(2012)
Article
Fisheries
Rosa M. Farto Seguin, Maria Bobo Bermudez, Leticia Rivera, Teresa P. Nieto
JOURNAL OF AQUATIC ANIMAL HEALTH
(2014)
Article
Microbiology
Estela Perez Lago, Teresa Perez Nieto, Rosa Farto Seguin
VETERINARY MICROBIOLOGY
(2009)
Article
Fisheries
Jose Antonio Guisande, Estela Perez Lago, Susana Prado, Teresa Perez Nieto, Rosa Farto Seguin
JOURNAL OF SHELLFISH RESEARCH
(2008)
Article
Chemistry, Applied
Pedro Alvarez-Boo, Jose S. Casas, Eduardo. E. Castellano, M. Deffina Couce, Rosa Farto, Eduardo Freijanes, Jose Sordo
APPLIED ORGANOMETALLIC CHEMISTRY
(2007)
Article
Fisheries
R. Farto, S. P. Armada, M. Montes, M. J. Perez, T. P. Nieto
JOURNAL OF FISH DISEASES
(2006)
Article
Fisheries
Rosa Farto, Jose Antonio Guisande, Susana P. Armada, Susana Prado, Teresa P. Nieto
JOURNAL OF SHELLFISH RESEARCH
(2006)