Article
Agriculture, Dairy & Animal Science
Kyung-Ho Kim, Kwang-Min Choi, Min-Soo Joo, Gyoungsik Kang, Won-Sik Woo, Min-Young Sohn, Ha-Jeong Son, Mun-Gyeong Kwon, Jae-Ok Kim, Do-Hyung Kim, Chan-Il Park
Summary: Red sea bream iridoviral disease (RSIVD) causes significant economic losses in the aquaculture industry. This study evaluated the dynamics of RSIV in rock bream under different water temperatures and RSIV inoculation concentrations. High viral copy numbers were observed during the period of fish mortality after RSIV infection at all concentrations in the tanks with high water temperatures. The study also found that the number of viral copies shed into the rearing seawater varied depending on the RSIV infection level in rock bream. These findings highlight the potential of non-invasive virus detection approaches and the quantitative analysis of seawater viruses in understanding and controlling fish diseases.
Article
Fisheries
Myung-Hwa Jung, Sajal Kole, Sung-Ju Jung
Summary: This study aims to develop a safe and efficient inactivated (IV) vaccine against rock bream iridovirus (RBIV). The evaluation results show that saponin (Sa) acts as an adjuvant to induce antiviral immune response and protect rock bream from RBIV infection. The Sa-adjuvanted IV vaccine significantly improves fish survivability through upregulation of IL1 beta, Mx, and PKR gene transcript. It provides strong protection against reinfection with RBIV.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Sungjae Ko, Jongwon Lim, Suhee Hong
Summary: A novel tumor necrosis factor gene was identified in rock bream, showing differential expression patterns in various tissues and regulation by different stimuli, suggesting its involvement in both innate and adaptive immunity in the fish species.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Min A. Jeong, Ye Jin Jeong, Kwang Il Kim
Summary: This study compared the virulence of two subtypes of red sea bream iridovirus (RSIV). The results showed that the subtype II was more infective and pathogenic than the mixed subtype I/II. The mortality rate and viral shedding ratio were higher in the subtype II group. Gene expression analysis also revealed differences between the two subtypes, supporting the difference in virulence.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Sujin Lee, Ahran Kim, Dahye Yoon, Seonghye Kim, Seohee Ma, Wonho Lee, Do-Hyung Kim, Heui-Soo Kim, Hee-Jae Cha, Yung Hyun Choi, Suhkmann Kim
Summary: This study investigated the metabolic responses in rock bream infected with RBIV using HR-MAS NMR spectroscopy, revealing significant metabolic pathways related to energy metabolism in the liver of heavily infected fish. Lightly infected fish may have survived from heavy infection after 3 weeks. RBIV induced rapid and high energy production in the spleen, while disturbances in metabolism related to the immunological response were observed in the kidney.
Article
Fisheries
Chalermkwan Eurlaphan, Reiko Nozaki, Motohiko Sano, Keiichiro Koiwai, Ikuo Hirono, Hidehiro Kondo
Summary: This study investigated the kinetics of red sea bream iridovirus and host gene expression during infection in rock bream (Oplegnathus fasciatus). The results showed that viral gene transcripts were detected at 3 and 5 days post-injection, and certain host genes were differentially expressed during the infection process. The downregulation of certain inflammation-related genes may contribute to the increased susceptibility of this fish to the virus.
JOURNAL OF FISH DISEASES
(2023)
Article
Virology
Myung-Hwa Jung, Chamilani Nikapitiya, Soo-Jin Kim, Hyun-Ja Han, Myoung-Sug Kim, Hye Sung Choi, Sung-Ju Jung
Summary: This study found that ankyrin repeat-containing DNA vaccine provides protection against Rock bream iridovirus (RBIV) infection in rock bream and induces immune responses. Moreover, the ANK gene may serve as a candidate for detecting RBIV infection and revealing its pathogenic mechanisms.
Article
Fisheries
Xiaohong Huang, Jingguang Wei, Qi Zheng, Ya Zhang, Weibin Zhu, Jiangchun Liu, Yunxia Hou, Qiwei Qin, Youhua Huang
Summary: The spotted knifejaw (Oplegnathus punctatus) is an emerging economical aquaculture species in China. In this study, an iridovirus-like disease outbreak (SKIV-SD) in the spotted knifejaw aquaculture industry in Shandong Province was characterized. The SKIV-SD virus, identified as an ISKNV-type megalocytivirus, caused severe mortality and pathological lesions in cultured spotted knifejaw, sharing genetic similarities with infectious spleen and kidney necrosis virus (ISKNV).
Article
Plant Sciences
Gyeongik Ahn, Joon-Yung Cha, Jeong Won Lee, Gyeongran Park, Gyeong-Im Shin, Shi-Jian Song, Gyeongryul Ryu, Inhwan Hwang, Min Gab Kim, Woe-Yeon Kim
Summary: In this study, a plant-based recombinant vaccine production system combined with surface display on L. lactis was developed. The recombinant protein targeting iridovirus was abundantly produced in N. benthamiana, purified using Ni-NTA resin and size exclusion chromatography, and successfully displayed immunogenicity in a mouse model through ELISA. These findings demonstrate the potential for utilizing this system in vaccine production against viral diseases.
JOURNAL OF PLANT BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Eun-Ji Ko, Hyunsu Kim, A-Reum Lee, Kyung-Yoon Jeon, Ahran Kim, Do-Hyung Kim, Chan-Il Park, Yung Hyun Choi, Suhkmann Kim, Heui-Soo Kim, Mee Sun Ock, Hee-Jae Cha
Summary: The protein expression patterns in spleens of rock bream after infection by RBIV or mixed infection were investigated. Proteins related to oxygen transport and energy generation were up-regulated in infected spleens, while proteins involved in structure and cell movement were down-regulated. Infection triggered energy generation and oxygen transport, but decreased cell migration and structural changes in the spleen.
Article
Biotechnology & Applied Microbiology
Jie Gong, Bijun Li, Ji Zhao, Zhixiong Zhou, Qiaozhen Ke, Qihui Zhu, Dongdong Xu, Tao Zhou, Peng Xu
Summary: In this study, a PCR method was developed to identify sex-specific sequences in rock bream using next-generation sequencing. Sex-specific genomic regions/loci were discovered on chromosomes 2 and 6, and candidate sex-determining genes were detected. A male-specific 34-bp deletion on chromosome 2 was identified as a molecular marker for sex determination in rock bream. This study provides insights into the mechanism of sex determination in rock bream and is important for future genomic research and aquaculture practice.
MARINE BIOTECHNOLOGY
(2022)
Article
Environmental Sciences
Peng Tan, Pian Zhang, Lei Zhang, Wenliang Zhu, Ligai Wang, Ruiyi Chen, Qihui Zhu, Dongdong Xu
Summary: This study investigates the effects of dietary soybean lecithin (SL) inclusion on the growth performance, liver metabolism, resistance to hypoxia stress, and potential molecular mechanisms in rock bream larvae. The results show that dietary SL inclusion promotes growth performance, survival rate, stress resistance, and improves intestinal structure. Additionally, a high SL inclusion diet increases the activity of lipolysis-related enzymes in liver tissue but decreases the activity of amino acid catabolism-related enzymes.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Mingtao Liu, Qihui Zhu, Huan Li, Ruiyi Chen, Weihua Hu, Simiao Liu, Dongdong Xu
Summary: We investigated the early gonadal development in rock bream through histological observations. Females showed earlier differentiation compared to males. Treatment with letrozole (LTZ) and 17 alpha-methyltestosterone (MT) resulted in complete masculinization of genotype-females, while 17beta-estradiol (E-2) only partially feminized genotype-males. These findings contribute to the understanding of sex determination and differentiation in fishes with the X1X2Y sex chromosome system and provide a sex reversal protocol for rock bream.
MARINE BIOTECHNOLOGY
(2023)
Article
Genetics & Heredity
Yulin Bai, Jie Gong, Zhixiong Zhou, Bijun Li, Ji Zhao, Qiaozhen Ke, Xiaoqing Zou, Fei Pu, Linni Wu, Weiqiang Zheng, Tao Zhou, Peng Xu
Summary: The Rock Bream is an economically important fish species in the Northwest Pacific Ocean and has been cultivated as an important edible fish in coastal areas of China. A chromosome-level reference genome of female Rock Bream was constructed using advanced sequencing technologies, providing essential resources for genetic studies and artificial selection mechanisms in marine aquaculture.
FRONTIERS IN GENETICS
(2021)
Article
Fisheries
W. S. P. Madhuranga, M. D. Neranjan Tharuka, J. C. Harasgama, Hyukjae Kwon, Qiang Wan, Jehee Lee
Summary: IFP35 plays important roles in antiviral defense, and its expression is upregulated in response to viral infections in rock bream. Overexpressed IFP35 can suppress VHSV transcription in vitro, and can activate the type I interferon pathway.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)