Article
Fisheries
Lan Zhang, Lei Wang, Jintai Huang, Zhan Jin, Junxiang Guan, Hang Yu, Meng Zhang, Miao Yu, Hongxia Jiang, Zhigang Qiao
Summary: In this study, the effects of A. hydrophila infection on the intestinal microbiota, transcriptome, and metabolome of Cyprinus carpio were investigated. The results showed significant changes in the intestinal microbiota, with a decrease in beneficial bacteria and an increase in pathogenic bacteria. The transcriptome analysis revealed altered expression of immune-related genes, and the metabolomic analysis indicated disturbances in amino acid metabolism. This study provides insight into the underlying mechanisms of the intestinal microbiota, immunity, and metabolism involved in the response of carp to A. hydrophila infection.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Morteza Yousefi, Seyyed Morteza Hoseini, Afaf N. Abdel Rahman, Yury Anatolyevich Vatnikov, Evgeny Vladimirovich Kulikov, Elena Valentinovna Kharlitskaya, Sergey Borisovich Seleznev
Summary: This study examines the effects of dietary limonene treatment on growth performance, immune response, and disease resistance in common carp. The results indicate that adding 200 mg/kg limonene to the diet improves feed efficiency, boosts innate immunity, and increases resistance against Aeromonas hydrophila.
Article
Fisheries
Zhao Jia, Jianhua Feng, Gaoliang Yuan, Hehe Xiao, Huifeng Dang, Yanwei Zhang, Kangyong Chen, Jun Zou, Junya Wang
Summary: Monoclonal antibodies against grass carp Metrnl-a were prepared and characterized in this study. The expression of CiMetrnl-a increased in grass carp infected with A. hydrophila, particularly in the gills, head kidney and intestine. CiMetrnl-a producing cells were mainly detected in the mucosal layer of the inflamed intestine caused by A. hydrophila infection. qRT-PCR analysis confirmed the marked induction of CiMetrnl-a mRNA expression. These findings suggest the involvement of CiMetrnl-a in regulating bacterial infection-induced intestine inflammation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Fisheries
Yanliang Jiang, Minghui Yu, Chuanju Dong, Jian Xu, Songhuan Chang, Qin Zhang, Jianxin Feng, Hanyuan Zhang, Youxiu Zhu, Biyin Wu
Summary: The common carp is an important aquaculture fish species farmed worldwide, but intensive rearing increases susceptibility to pathogens. Research found that resistance of common carp to Aeromonas hydrophila infection is related to multiple immune-related genes.
Article
Fisheries
Yanjing Zhao, Jie Zhang, Dan Qiao, Feng Gao, Xinyu Jiang, Xianliang Zhao, Libo Hou, Hao Li, Li Li, Xianghui Kong
Summary: In this study, the molecular and structural characteristics of CcGSDMEa-like were analyzed, and its role in the inflammatory response against Aeromonas hydrophila in fish was studied. The results showed that CcGSDMEa-like could regulate the content of bacteria in fish through mediating the expression and secretion of CcIL-1 beta.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Immunology
Yunchao Cao, Tianshun Kou, Liaotian Peng, Hetron Mweemba Munang'andu, Bo Peng
Summary: Aquatic food is an important source of micronutrients for human beings, and aquaculture plays an increasingly important role in providing this food source. However, bacterial infection poses a severe threat to aquaculture and strategies for managing these infections without antibiotics are lacking. This study investigates the metabolic changes of crucian carp infected with Aeromonas hydrophila and identifies fructose as a crucial biomarker. Supplementation of fructose increases fish survival rate by enhancing the expression of lysozyme and complement 3, leading to reduced bacterial load. These findings demonstrate a metabolism-based approach to manage bacterial infection by modulating the immune response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Songhuan Chang, Jiali Wang, Chuanju Dong, Yanliang Jiang
Summary: Common carp is an important freshwater farmed fish species worldwide and is susceptible to pathogen Aeromonas hydrophila. In this study, 16S rDNA sequencing was used to analyze the composition and biodiversity of intestinal microbiota in common carp following A. hydrophila infection. The results showed that the diversity and richness of microbiota decreased after infection, and the relative abundance of specific genera was significantly altered. Further analysis revealed potential correlations between certain genera and host resistance against pathogens. This study contributes to our understanding of the underlying mechanisms of intestinal microbial community in resisting pathogens and may provide strategies for disease control in aquaculture.
AQUACULTURE REPORTS
(2023)
Article
Immunology
Qingjiang Mu, Zhaoran Dong, Weiguang Kong, Xinyou Wang, Jiaqian Yu, Wei Ji, Jianguo Su, Zhen Xu
Summary: This study investigates the immune response of gut mucosal IgM during bacterial invasion using a common carp model. The results show that IgM(+) B cells are strongly induced in the gut and head kidney upon bacterial infection, and bacteria-specific IgM responses are detected in high levels in both the gut mucus and serum. These findings reveal the crucial role of IgM in the defense mechanisms of the gut against bacterial invasion.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Yang Zhou, Lin Feng, Wei-Dan Jiang, Pei Wu, Yang Liu, Jun Jiang, Sheng-Yao Kuang, Ling Tang, Yan Peng, Xiao-Qiu Zhou
Summary: The study indicated that dietary cinnamaldehyde improved the intestinal immune function of grass carp by enhancing the activity of lysozyme and acid phosphatase, increasing complement and immunoglobulin levels, and regulating the expression of various immune-related genes. Moreover, optimal levels of CIN supplementation were estimated based on specific indicators for C. idella.
AQUACULTURE REPORTS
(2021)
Article
Virology
Lingting Pan, Dengfeng Li, Wei Lin, Wencai Liu, Chenxin Qu, Minhua Qian, Ruqian Cai, Qin Zhou, Fei Wang, Yigang Tong
Summary: Ahy-yong1 is a newly discovered phage that can effectively treat multi-antibiotic-resistant pathogen A. hydrophila. It has showed strong biofilm removal capacity and protective effect in fish.
Article
Microbiology
Pengji Zhou, Haiyan Huang, Jiaoyang Lu, Zirong Zhu, Junyan Xie, Liqiu Xia, Sisi Luo, Kexuan Zhou, Wenhui Chen, Xuezhi Ding
Summary: The study demonstrated the enhanced antibacterial effect of BaX030 through compound mutagenesis, leading to potential applications in aquaculture. The mutated strain N-11 showed significant increase in the production of macrolactin A and oxydifficidin, as well as elevated expression of immune factors in grass carp. The study identified a probiotic strain with promising antibacterial activity for aquaculture production, offering a new strategy for discovering strains with higher biological activity.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Fisheries
Jianjun Chen, Yidi Zhao, Dandan Sun, Chenyang Rao, Lulu Li, Suqi Guo, Shuai Yang, Xianglin Cao
Summary: This study demonstrates that dietary compound probiotics can enhance intestinal function and disease resistance in common carp infected with A. hydrophila, while also preserving muscle quality.
AQUACULTURE INTERNATIONAL
(2022)
Article
Fisheries
Licheng Yin, Wenting Xu, Xuelian Liu, Yueyue Wang, Pei Ge, Xinyan Wang, Hong Zhou
Summary: The study showed that A. hydrophila infection significantly induced autophagy in grass carp monocytes/macrophages, and regulating autophagy can affect the survival of the pathogen. These findings help us better understand the interaction between A. hydrophila and host cells.
Article
Fisheries
Lili Yun, Meiru Kang, Yihao Shen, Junchang Feng, Guokun Yang, Jianxin Zhang, Xiaolin Meng, Xulu Chang
Summary: This study evaluated the effects of dietary supplementation with Bacillus velezensis R-71003 and sodium gluconate on antioxidant capacity, immune response, and resistance against Aeromonas hydrophila in common carp. The results showed that the antibacterial crude extract of B. velezensis R-71003 can destroy the cell wall of A. hydrophila. Additionally, dietary B. velezensis R-71003 and sodium gluconate improved the antioxidant capacity and immunity of common carp.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Bing-Yao Sun, Wen He, Hui-Xin Yang, Dan-Yang Tian, Pan-Yang Jian, Kang Wu, Cai-Gen Yang, Xue-Hong Song
Summary: This study using a grass carp model found that intestinal dysbiosis increased the susceptibility of fish to bacterial infection, suggesting it as a risk factor for severe bacterial infection in cultured fish.
Article
Fisheries
M. Adamek, J. Hellmann, V. Jung-Schroers, F. Teitge, D. Steinhagen
JOURNAL OF FISH DISEASES
(2018)
Article
Fisheries
M. Adamek, F. Baska, B. Vincze, D. Steinhagen
JOURNAL OF FISH DISEASES
(2018)
Article
Fisheries
Mikolaj Adamek, Felix Teitge, Verena Jung-Schroers, Max Heling, David Gela, Veronika Piackova, Martin Kocour, Dieter Steinhagen
JOURNAL OF FISH DISEASES
(2018)
Article
Fisheries
Verena Jung-Schroers, Mikolaj Adamek, Angela Boley, Anna Korshun, Dieter Steinhagen
JOURNAL OF FISH DISEASES
(2019)
Article
Fisheries
Verena Jung-Schroers, Arne Jung, Martin Ryll, Julia Bauer, Felix Teitge, Dieter Steinhagen
JOURNAL OF FISH DISEASES
(2019)
Article
Fisheries
Snjezana Zrncic, Drazen Oraic, Ivana Giovanna Zupicic, Zeljko Pavlinec, Dragan Brnic, Zaklin Acinger Rogic, Ivica Sucec, Dieter Steinhagen, Mikolaj Adamek
JOURNAL OF FISH DISEASES
(2020)
Article
Veterinary Sciences
Verena Jung-Schroers, Uta Hildebrandt, Karina Retter, Karl-Heinz Esser, John Hellmann, Dirk Willem Kleingeld, Karl Rohn, Dieter Steinhagen
BMC VETERINARY RESEARCH
(2020)
Article
Fisheries
Felix Teitge, Christina Peppler, Dieter Steinhagen, Verena Jung-Schroers
JOURNAL OF FISH DISEASES
(2020)
Article
Microbiology
L'ubomir Pojezdal, Mikolaj Adamek, Eva Syrova, Dieter Steinhagen, Hana Minarova, Ivana Papezikova, Veronika Seidlova, Stanislava Reschova, Miroslava Palikova
Article
Fisheries
Felix Teitge, Christina Peppler, Dieter Steinhagen, Verena Jung-Schroers
JOURNAL OF FISH DISEASES
(2020)
Article
Fisheries
Julia Bauer, Felix Teitge, Lisa Neffe, Mikolaj Adamek, Arne Jung, Christina Peppler, Dieter Steinhagen, Verena Jung-Schroers
Summary: In land-based recirculating aquaculture systems, low water salinities can impact the disease resistance of shrimp and alter the microbial composition, posing a higher risk of disease outbreaks and potentially affecting food safety. Maintaining high water salinities is recommended for producing healthy shrimp as safe food for human consumption.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Martin Felten, Mikolaj Adamek, Marina Gebert, Sebastian Rakers, Dieter Steinhagen
Summary: Cell characteristics and immune responses have an influence on the susceptibility of carp cell lines to viral infection. Cells with lower levels of type I interferon responses may be more susceptible to replication of certain viruses.
JOURNAL OF FISH DISEASES
(2022)
Article
Microbiology
Stephanie Gross, Anja Mueller, Diana Seinige, Manuela Oliveira, Dieter Steinhagen, Ursula Siebert, Corinna Kehrenberg
Summary: This study investigates the occurrence of antimicrobial-resistant Escherichia coli in free-living great cormorants in Germany. The results show that resistant E. coli were isolated from a significant proportion of the samples, and a substantial number of the resistant isolates exhibited multidrug resistance. Most of the isolates belonged to the phylogenetic group B1, and a portion of them were identified as avian pathogenic E. coli with multidrug resistance. These findings provide valuable information on the dissemination of antimicrobial-resistant bacteria in wild birds and potential public health concerns.
Article
Infectious Diseases
Stephanie Gross, Anja Mueller, Diana Seinige, Peter Wohlsein, Manuela Oliveira, Dieter Steinhagen, Corinna Kehrenberg, Ursula Siebert
Summary: Antimicrobial resistance is a global health threat with wildlife acting as a reservoir and vector for resistant bacteria and genes. This study found resistant strains of Escherichia coli in marine mammals of the North and Baltic Seas, highlighting their role as sentinels for antimicrobial-resistant bacteria. The distribution of resistant bacteria in these sea areas provides important information from a One Health perspective.
Review
Immunology
Japhette E. E. Kembou-Ringert, Dieter Steinhagen, John Readman, Janet M. M. Daly, Mikolaj Adamek
Summary: Tilapia tilapinevirus (TiLV) is a newly emerged virus that causes a novel disease and reduces tilapia populations worldwide. It has been reported in 17 countries, causing high mortalities. Currently, there are no therapeutics or vaccines specifically designed for TiLV control. Vaccination has shown potential in inducing protective immunity in tilapia. Various vaccine strategies are being explored, but a comprehensive overview of their efficacy is lacking. This review discusses recent advances in TiLV vaccine development, highlighting the need for further research and the incorporation of different approaches to enhance vaccine effectiveness.