Journal
DISEASE MODELS & MECHANISMS
Volume 5, Issue 6, Pages 956-966Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dmm.009696
Keywords
-
Categories
Funding
- National Institutes of Health Collaborative Bridging Project grant from the Beta Cell Biology Consortium [U01 DK072473, U19 DK072495, U01 DK072503]
- Novo Nordisk Foundation Section for Basic Stem Cell Biology [Serup Group NNF] Funding Source: researchfish
Ask authors/readers for more resources
Extracellular signals in development, physiology, homeostasis and disease often act by regulating transcription. Herein we describe a general method and specific resources for determining where and when such signaling occurs in live animals and for systematically comparing the timing and extent of different signals in different cellular contexts. We used recombinase-mediated cassette exchange (RMCE) to test the effect of successively deleting conserved genomic regions of the ubiquitously active Rosa26 promoter and substituting the deleted regions for regulatory sequences that respond to diverse extracellular signals. We thereby created an allelic series of embryonic stem cells and mice, each containing a signal-responsive sentinel with different fluorescent reporters that respond with sensitivity and specificity to retinoic acids, bone morphogenic proteins, activin A, Wnts or Notch, and that can be adapted to any pathway that acts via DNA elements.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available