Article
Cell Biology
Henrika Jodeleit, Paula Winkelmann, Janina Caesar, Sebastian Sterz, Lesca M. Holdt, Florian Beigel, Johannes Stallhofer, Simone Breiteneicher, Eckart Bartnik, Thomas Leeuw, Matthias Siebeck, Roswitha Gropp
Summary: This study aimed to demonstrate patient stratification for oxelumab treatment using immune profiling and a humanized mouse model of UC. Results showed significant differences in OX40L expression levels between UC and non-UC donors. The efficacy of oxelumab in reducing clinical scores and inflammation was slightly better than adalimumab, suggesting a potential benefit for patients with elevated OX40L levels.
DISEASE MODELS & MECHANISMS
(2021)
Article
Immunology
Yiteng Lu, Ye Sun, Yong Peng, Xiaoqiang Zhao, Danjie Wang, Tongtong Zhang, Fang Qian, Jun Wang
Summary: The role of Gasdermin D (GSDMD) in atopic dermatitis (AD) was investigated. Through analysis of gene expression data and mouse experiments, it was found that GSDMD deletion and inhibition can alleviate symptoms of AD, such as reduced skin thickness, scarring, and scratching behavior. Furthermore, GSDMD deletion and inhibition also resulted in reduced infiltration of inflammatory cells, decreased epidermal thickness, and lower levels of IL-18 and IL-18.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Dermatology
Yu Wang, Chaoying Gu, Shangshang Wang, Huibin Yin, Ronghui Zhu, Ce Wang, Zheng Li, Xu Yao, Wei Li
Summary: This study explored the transcriptome signature of PBMCs from Chinese patients with atopic dermatitis (AD) and identified two potential genes for predicting the efficacy of dupilumab.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2023)
Article
Chemistry, Medicinal
Tae-Kyeong Lee, Dae Won Kim, Ji Hyeon Ahn, Choong-Hyun Lee, Jae-Chul Lee, Soon Sung Lim, Il Jun Kang, Seongkweon Hong, Soo Young Choi, Moo-Ho Won, Joon Ha Park
Summary: Topical administration of laminarin improved oxazolone-induced atopic dermatitis-like skin lesions in Balb/c mice by reducing swelling, decreasing epidermal and dermal thickness, inhibiting mast cell infiltration, and lowering IgE levels. It also suppressed the expression of proinflammatory cytokines in the skin lesion, suggesting laminarin's potential as a candidate for atopic dermatitis treatment.
Article
Multidisciplinary Sciences
Sarita Kanwal, Shanker K. Singh, Sandeep P. Soman, Soumen Choudhury, Priyambada Kumari, Pradeep K. Ram, Satish K. Garg
Summary: In this study, it was found that dogs with AD have significantly higher expression of FLG gene and significantly lower expression of IVL gene in the skin, in addition to significantly increased expression of TNF-alpha, IL-31, and IL-13 genes in their peripheral blood mononuclear cells. These findings suggest that the disruption of skin barrier in dogs with AD may be attributed to the altered expression of FLG and IVL genes, while the clinical manifestations of AD may be associated with the overexpression of TNF-alpha, IL-31, and IL-13 genes. Further studies are needed to confirm these findings and explore the pathogenesis of AD in dogs.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Anna-Lena Unterweger, Alena Ruescher, Marietta Seuss, Paula Winkelmann, Florian Beigel, Leandra Koletzko, Simone Breiteneicher, Matthias Siebeck, Roswitha Gropp, Attila Aszodi
Summary: The combined approach of patient immune-profiling and a mouse model based on NSG reconstituted with PBMC from CD patients partially reflects the human disease, showing an immune-remodeling phenotype with increased collagen deposition, elevated levels of specific immune cells, and inflammatory markers. This model provides a platform for studying fibrosis development in CD.
IMMUNITY INFLAMMATION AND DISEASE
(2021)
Article
Integrative & Complementary Medicine
Ramin Goudarzi, Maryam Eskandarynasab, Ahad Muhammadnejad, Ahmad Reza Dehpour, Alireza Partoazar
Summary: The study demonstrated that ROCEN had similar therapeutic effects to Beta in an experimental mouse model of atopic dermatitis, significantly reducing scratching and wiping symptoms, improving skin conditions, reducing leucocyte infiltration, and decreasing levels of inflammatory cytokines.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2021)
Review
Allergy
Mitsutoshi Tominaga, Kenji Takamori
Summary: Atopic dermatitis is a skin disorder characterized by dryness and dysfunction of the skin barrier, leading to inflammation and chronic itch. The itch sensation is sensitized by the increase in intraepidermal nerve fibers and the secretion of IL-33 by keratinocytes. The breakdown of the skin barrier allows allergens to enter the epidermis, triggering type 2 inflammatory responses and worsening itch. Controlling itch is crucial for the treatment of atopic dermatitis.
ALLERGOLOGY INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Banzragch Dorjsembe, Chu Won Nho, Yongsoo Choi, Jin-Chul Kim
Summary: Black soybean cultivar A63 has higher contents of beneficial compounds and shows potential in treating atopic dermatitis symptoms and restoring skin barrier function.
Article
Biochemistry & Molecular Biology
Vladimir-Andrey Gimenez-Rivera, Harshita Patel, Franck P. Dupuy, Zoulfia Allakhverdi, Charlie Bouchard, Joaquin Madrenas, Robert Bissonnette, Ciriaco A. Piccirillo, Carolyn Jack
Summary: By investigating the effects of S. aureus-derived molecules and skin alarmins on human peripheral blood mononuclear cells, this study identified six significantly elevated Th2-related chemokine biomarkers in the blood of adult AD patients, which were associated with disease severity. In addition, the study demonstrated the potential of NOD2 as a regulator of type 2 immune responses and a therapeutic target for AD.
Article
Veterinary Sciences
Yuyo Ka, Ryoji Ito, Ryoko Nozu, Kayo Tomiyama, Masami Ueno, Tomoyuki Ogura, Riichi Takahashi
Summary: Humanized mice are widely used to study the human immune system and therapeutic targets for human diseases. This study established a new model of germ-free humanized mice and found that the human gut microbiota supports T cell proliferation and maintenance in the mice. This dual-humanized mouse model may be useful for studying the role of the gut microbiota in human immunity and as a new model in cancer immunology.
EXPERIMENTAL ANIMALS
(2023)
Article
Biochemistry & Molecular Biology
Zhiping Lu, Song Xiao, Weiwei Chen, Renkai Zhu, Hua Yang, Martin Steinhoff, Yanqing Li, Wenke Cheng, Xinrong Yan, Lianlian Li, Shanghai Xue, Ciara Larkin, Wenhao Zhang, Qianqian Fan, Ruizhen Wang, Jiafu Wang, Jianghui Meng
Summary: IL-20 expression in skin is upregulated in atopic dermatitis, and it triggers calcium influx in keratinocytes and sensory neurons, leading to release of AD-related molecules and transcription of itch-related genes. In a murine model, co-injection of IL-20 and IL-13 elicits significant itch-like behavior.
Article
Pharmacology & Pharmacy
Jaehee Lee, Leejin Park, Hyeyoung Kim, Bong-il Rho, Rafael Taeho Han, Sewon Kim, Hee Jin Kim, Heung Sik Na, Seung Keun Back
Summary: This study investigated the therapeutic effects of adipose-derived stem cells (ASCs) on atopic dermatitis (AD) rats. The results showed that systemic administration of ASCs can alleviate AD-like symptoms, restore the phagocytic activity of peripheral blood mononuclear cells (PBMCs), decolonize cutaneous Staphylococcus aureus, and improve the skin condition of AD rats.
KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
(2022)
Article
Dermatology
Ana Rostaher, Sabrina Rodriguez-Campos, Peter Deplazes, Lena Zwickl, A. Cezmi Akdis, Adrian Urwyler, David W. Wheeler, Sabrina Audergon, Nina Maria Fischer, Claude Favrot
Summary: The percentages of Treg cells were higher in healthy dogs compared to those that developed atopic dermatitis. Additionally, there was a positive correlation between the relative abundance of Lachnospiraceae on the skin and Treg cells in allergic puppies. Larger studies are needed to determine the practical implications of these findings for the diagnosis, treatment, and prevention of atopic dermatitis.
VETERINARY DERMATOLOGY
(2021)
Article
Dermatology
Fitria Salim, Hendra Gunawan, Oki Suwarsa, Endang Sutedja
Summary: This study investigates the increased expression of TLR2 and TLR6 on peripheral blood monocytes induced by SEB during exacerbation in AD patients.
CLINICAL COSMETIC AND INVESTIGATIONAL DERMATOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Vera A. van der Weijden, Meret Schmidhauser, Mayuko Kurome, Johannes Knubben, Veronika L. Floter, Eckhard Wolf, Susanne E. Ulbrich
Summary: Our study found that in vivo developed and in vitro produced embryos show similar transcriptome profiles during development. Shared canonical pathways in transitions from 4-cell to morula included oxidative phosphorylation and EIF2 signaling, while shared pathways from morula to hatched blastocyst included 14-3-3-mediated signaling, xenobiotic metabolism general signaling pathway, and NRF2-mediated oxidative stress response. Hatched blastocysts from in vitro fertilization-pipeline exhibited molecular pathways associated with lower developmental competence compared to in vivo developed embryos.
Review
Biochemistry & Molecular Biology
Christian Kupatt, Alina Windisch, Alessandra Moretti, Eckhard Wolf, Wolfgang Wurst, Maggie C. Walter
Summary: Mutations in the Dystrophin gene cause Duchenne Muscular Dystrophy, a severe muscle disease affecting both skeletal muscles and the heart. Various molecular therapies have been developed, and genome editing using CRISPR-Cas9 offers a novel option for correcting these mutations.
Article
Multidisciplinary Sciences
Beate Rieblinger, Hicham Sid, Denise Duda, Tarik Bozoglu, Romina Klinger, Antonina Schlickenrieder, Kamila Lengyel, Krzysztof Flisikowski, Tatiana Flisikowska, Nina Simm, Alessandro Grodziecki, Carolin Perleberg, Andrea Bahr, Lucie Carrier, Mayuko Kurome, Valeri Zakhartchenko, Barbara Kessler, Eckhard Wolf, Lutz Kettler, Harald Luksch, Ibrahim T. Hagag, Daniel Wise, Jim Kaufman, Benedikt B. Kaufer, Christian Kupatt, Angelika Schnieke, Benjamin Schusser
Summary: Research on genetically modified animals has mostly focused on mice, but also includes species like pigs that are more physiologically similar to humans, and cross-species comparisons with phylogenetically distant species like chickens. CRISPR-Cas9 is a versatile genetic editing method applicable across various species. The successful generation of transgenic chickens and pigs that express Cas9 in all organs has confirmed the functionality of Cas9 across different target genes, cell types, and in vivo applications.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Michael Walz, Christine Hoeflich, Christina Walz, Daniela Ohde, Julia Brenmoehl, Mandy Sawitzky, Andreas Vernunft, Uwe K. Zettl, Susanne Holtze, Thomas B. Hildebrandt, Eckhard Wolf, Andreas Hoeflich
Summary: The bioactivity of the IGF system is regulated by IGFBPs, IGFBP proteases, and inhibitors. The effects of the IGF system on the AKT/mTOR pathway are of interest. Using IGFBP2 to enhance the sensitivity of IGF-dependent bioactivity, phosphorylation of AKT-Ser473 was assessed as a readout of pathway activity.
Article
Endocrinology & Metabolism
Felicia Gerst, Elisabeth Kemter, Estela Lorza-Gil, Gabriele Kaiser, Ann-Kathrin Fritz, Rita Nano, Lorenzo Piemonti, Marie Gauder, Andreas Dahl, Silvio Nadalin, Alfred Koenigsrainer, Falko Fend, Andreas L. Birkenfeld, Robert Wagner, Martin Heni, Norbert Stefan, Eckhard Wolf, Hans-Ulrich Haering, Susanne Ullrich
Summary: The decline of fetuin-A levels during perinatal period may relieve TGFBR signaling in islets, facilitating functional maturation of neonatal beta cells. However, elevated plasma fetuin-A levels in later life can impair both function and adaptive proliferation of beta cells, leading to a reduction in functional beta cell mass and an acceleration of the onset of type 2 diabetes.
Article
Multidisciplinary Sciences
Natalie Theobalt, Isabel Hofmann, Sonja Fiedler, Simone Renner, Georg Dhom, Annette Feuchtinger, Axel Walch, Martin Hrabe de Angelis, Eckhard Wolf, Ruediger Wanke, Andreas Blutke
Summary: In translational obesity research, LSFM is demonstrated as an effective tool for accurate analysis of adipocyte volumes and numbers, providing valuable data for evaluating intervention efficacies.
Article
Cell Biology
Daniela Bebbere, Susanne E. Ulbrich, Katrin Giller, Valeri Zakhartchenko, Horst-Dieter Reichenbach, Myriam Reichenbach, Paul J. Verma, Eckhard Wolf, Sergio Ledda, Stefan Hiendleder
Summary: The study reveals about 50% reduction in mitochondrial DNA (mtDNA) in the liver and skeletal muscle of SCNT fetuses at day 80 of gestation, with no significant decrease observed in the brain. The depletion of mtDNA is associated with hepatomegaly and muscle hypertrophy of SCNT fetuses, indicating that it is a major signature of perturbations after SCNT. The expression of selected nuclear-encoded genes pivotal for mtDNA replication is similar to controls, suggesting that the mitochondrial perturbation in interaction with incomplete nuclear reprogramming drives abnormal epigenetic features and correlated phenotypes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Genetics & Heredity
Sylvain Bertho, Amaury Herpin, Elodie Jouanno, Ayaka Yano, Julien Bobe, Hugues Parrinello, Laurent Journot, Rene Guyomard, Thomas Muller, Penny Swanson, Garrett McKinney, Kevin Williamson, Mariah Meek, Manfred Schartl, Yann Guiguen
Summary: This study found that some XY Chinook salmon females carry a mutated sdY gene, which leads to changes in the protein structure and function, affecting testicular differentiation and estrogen synthesis in Chinook salmon.
G3-GENES GENOMES GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Claudia Siverino, Shorouk Fahmy-Garcia, Didem Mumcuoglu, Heike Oberwinkler, Markus Muehlemann, Thomas Mueller, Eric Farrell, Gerjo J. V. M. van Osch, Joachim Nickel
Summary: This study proposes an alternative strategy for the treatment of large bone defects by covalently immobilizing an engineered BMP2 variant to biocompatible scaffolds. The results demonstrate the translational potential of this innovative technique in inducing bone tissue formation, improving safety for patients, and reducing costs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sarah Duin, Shreya Bhandarkar, Susann Lehmann, Elisabeth Kemter, Eckhard Wolf, Michael Gelinsky, Barbara Ludwig, Anja Lode
Summary: The transplantation of pancreatic islets can prevent complications in diabetes patients. 3D bioprinting can encapsulate islets and improve the success rate of transplantation. This study successfully bioprinted neonatal porcine islet-like cell clusters and demonstrated their functionality in vitro.
Article
Nutrition & Dietetics
Rosamaria Lugara, Simone Renner, Eckhard Wolf, Annette Liesegang, Rupert Bruckmaier, Katrin Giller
Summary: Excessive intake of fats and sugars in the Western diet is a major cause of obesity. This study investigated the effects of Western diet and spirulina supplementation on gestating and lactating domestic pigs as a model for metabolic disturbances. The results suggest that domestic pigs have limited suitability as a model for Western diet-induced metabolic impairments, but low-dose spirulina supplementation can partially attenuate the negative effects.
Article
Biochemistry & Molecular Biology
Elena E. E. Wolf, Anne Steglich, Friederike Kessel, Hannah Kroeger, Jan Sradnick, Simone Reichelt-Wurm, Kathrin Eidenschink, Miriam C. C. Banas, Eckhard Wolf, Ruediger Wanke, Florian Gembardt, Vladimir T. T. Todorov
Summary: PLVAP serves as an early marker of glomerular endothelial injury in diabetic kidney disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Valerie Pauly, Julia Vlcek, Zhihao Zhang, Nora Hesse, Ruibing Xia, Julia Bauer, Simone Loy, Sarah Schneider, Simone Renner, Eckhard Wolf, Stefan Kaeaeb, Dominik Schuettler, Philipp Tomsits, Sebastian Clauss
Summary: Atrial fibrillation (AF) is a common arrhythmia caused by myocardial ischemia/infarction (MI). Men have a higher prevalence of AF, while women have a higher risk of new onset AF after MI. The impact of sex on AF pathophysiology is largely unknown. In pigs with/without ischemic heart failure (IHF), both male and female pigs showed increased vulnerability to AF. Male pigs had more and longer AF episodes, while female pigs had sinus node dysfunction.
Article
Biochemistry & Molecular Biology
Marie-Christin Schilloks, Isabella-Maria Giese, Arne Hinrichs, Lucia Korbonits, Stefanie M. Hauck, Eckhard Wolf, Cornelia A. Deeg
Summary: This study investigated the effects of impaired GHR signaling on immune functions and immunometabolism in GHR-KO pigs. The results showed significant differences in the relative proportion of the CD4(+)CD8a(-) subpopulation and IFN-a levels between GHR-KO pigs and WT controls. However, no significant difference was found in the respiratory capacity and the capacity for polyclonal stimulation in PBMCs between the two groups. Proteome analysis revealed multiple significant protein abundance differences related to amino acid metabolism, beta-oxidation of fatty acids, insulin secretion signaling, and oxidative phosphorylation between GHR-KO pigs and WT pigs.
Article
Biochemistry & Molecular Biology
Veronika Wess, Paula Schuster-Winkelmann, Yasemin Hazal Karatekin, Simge Malik, Florian Beigel, Florian Kuehn, Roswitha Gropp
Summary: Animal models, such as NSG-UC and NSG-CD mice, can partially mimic the pathologies of ulcerative colitis (UC) and Crohn's disease (CD) in humans. NSG-UC mice exhibit a strong inflammatory response when challenged with ethanol, while NSG-CD mice develop pathological manifestations without challenge.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)