Article
Biochemical Research Methods
Martin Lycka, Vratislav Peska, Martin Demko, Ioannis Spyroglou, Agata Kilar, Jiri Fajkus, Miloslava Fojtova
Summary: This study introduces a new online toolset called WALTER for the rapid, reproducible, and user-friendly analysis of telomere length data and statistical evaluation, representing a major upgrade in TRF scan image processing.
BMC BIOINFORMATICS
(2021)
Article
Microbiology
Cheng-Hung Lai, Yu-Sin Lin, Chao-Min Wang, Poa-Chun Chang, Wei-Yau Shia
Summary: This study identified Capnocytophaga strains in the oral cavity of dogs using 16S rRNA and phylogenetic analysis. A novel 16S rRNA PCR-RFLP method was designed to accurately distinguish C. cynodegmi from C. canimorsus, improving detection accuracy. This method is important for the epidemiological study of Capnocytophaga and the rapid diagnosis of human C. canimorsus infections following exposure to small animals.
MICROBIOLOGY SPECTRUM
(2023)
Article
Pharmacology & Pharmacy
R. R. Al-Hindi, A. Bahieldin, S. Edris, Khloud Mubarak Algothmi, Sajaawad Al Salami, Mona Ghonaim Alharbi, A. D. Teklemariam, T. Karamitros, T. Abujamel, S. Harakeh
Summary: The in silico approach using restriction enzymes analysis of 16S gene was used to identify lactic acid bacteria and detect species-specific markers. The study showed that this method can provide better understanding of organisms found in raw and/or fermented milk.
INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Entomology
Zhi-Chao Yan, Hai-Qing Hua, Guang-Yuan Qi, Yuan-Xi Li
Summary: We developed a method to detect and identify Trichogramma larvae in their host eggs and successfully differentiated eight Trichogramma species using a PCR-RFLP assay. This study provides a promising approach for conveniently identifying Trichogramma in insectaries and accurately evaluating parasitism rates in the field.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2022)
Article
Microbiology
Charlotte Marie Ahle, Kristian Stodkilde-Jorgensen, Anja Poehlein, Wolfgang R. Streit, Jennifer Hupeden, Holger Bruggemann
Summary: This study compared three amplicon NGS schemes targeting different regions within the genes tuf and rpsK to determine staphylococcal populations in skin samples. The novel tuf2-NGS scheme exhibited superior resolution power, allowing for accurate identification of specific Staphylococcus species. Powerful amplicon NGS approaches can be useful for detecting and quantifying staphylococci in human samples, providing valuable information for research on their roles in health and disease.
Article
Plant Sciences
Soon Jeong Lee, Sang-Rae Lee
Summary: This study developed a species-specific molecular marker for distinguishing between two red rot disease pathogens. Without sequencing, the polymerase chain reaction restriction fragment length polymorphism method was used to classify Pythium species at the species level. The developed molecular markers are effective for long-term monitoring of infection and distribution patterns in Pyropia aquaculture farms.
Article
Plant Sciences
Harleen Kaur, Jhulia Gelain, Marcus Vinicius Marin, Natalia A. Peres, Guido Schnabel
Summary: A new Neopestalotiopsis sp. has been discovered in strawberry plants in Florida, Georgia, and South Carolina, causing severe leaf spot and fruit rot. This new species is more aggressive and destructive than other Pestalotiopsis pathogens, leading to significant yield losses for affected growers. The study developed a molecular method based on PCR/RFLP for accurate and fast identification of the new Neopestalotiopsis sp. variant from strawberry plants. This method utilizes PCR amplification of a beta-tubulin gene fragment and the restriction enzyme BsaWI to differentiate the new species from others.
Article
Chemistry, Multidisciplinary
Gregor Drago Zupancic, Mario Panjicko, Romana Marinsek Logar, Lea Lavric, Masa Zorec, Lijana Fanedl
Summary: This study conducted two experiments to observe the responses of bacterial and archaeal communities to hypersaline environments. The results showed that methane production could recover after the salt concentration decreased in the gradual addition of salt experiment. The bacterial community had a good recovery during rapid salt addition, while salt-sensitive species decreased in the archaeal community. Certain bacterial and archaeal communities were well-adapted to the hypersaline environment and remained active during anaerobic digestion with high salt concentration.
APPLIED SCIENCES-BASEL
(2023)
Article
Biochemistry & Molecular Biology
T. Shakena Fathima, S. John Adams, Anju Majeed, G. Muralitharan, N. Thajuddin
Summary: Due to morphological convergence and taxonomic concepts, the systematics of filamentous cyanobacteria remains a problem. This study evaluated the use of 16S rRNA gene sequences to infer genetic relationships among freshwater cyanobacterial isolates, and the taxonomic resolution was determined through phylogenetic analysis, restriction digestion, and structure prediction.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Jenna T. B. Ekwealor, Simone D. Benjamin, Jordan Z. Jomsky, Matthew A. Bowker, Lloyd R. Stark, D. Nicholas McLetchie, Brent D. Mishler, Kirsten M. Fisher
Summary: This study presents a technique to determine the genotypic sex of S. caninervis moss and examines the genotypic and phenotypic sex ratios in a case study. The results show that the genotypic ratio of non-expressing shoots is female-biased and exceeds the phenotypic ratio. Additionally, the distribution of male and female genotypes is not predicted by sex expression patterns in different microhabitats.
APPLICATIONS IN PLANT SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Nada K. Sedky, Reem K. Arafa
Summary: Novel Biochemistry lab techniques are rapidly being introduced in scientific research, requiring the development of new labs for undergraduate Biochemistry courses. However, the time limit of Biochemistry labs for undergraduate students poses a major obstacle. This article presents a clear set of laboratory exercises designed to introduce students to the use of PCR-RFLP as a means of detection of genetic variants. Three consecutive lab sessions have been designed for the undergraduate students to learn and practice this technique.
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION
(2022)
Article
Dentistry, Oral Surgery & Medicine
Hiroto Sano, Anna Wakui, Miho Kawachi, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Keiko Yamaki, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato
Summary: This study developed a simple and cost-effective method for profiling oral microbiota using PCR-RFLP analysis of PCR-amplified 16S rRNA genes. The analysis successfully identified 65 RFLP patterns representing 27 genera and 59 species from human saliva. The PCR-RFLP method is a cost-effective alternative for oral microbiota analysis in smaller laboratories.
JOURNAL OF ORAL BIOSCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Abdelfattah Selim, Hanem Khater
Summary: This study identified two distinct circulating genotypes of T. annulata among cattle in Egypt using PCR-RFLP based on the HSP 70 gene. The T. annulata isolates in the northern and southern governorates exhibited different patterns of fragment lengths.
Article
Pediatrics
Koert de Waal, Rahul Prasad, Martin Kluckow
Summary: The literature on PDA management is challenging due to poorly designed trials with high rates of open label treatments. Therapeutic nihilism has led to a drift away from medical and surgical treatment, failing to recognize the physiological relevance of a left-to-right shunt. The safety of therapeutic nihilism needs to be further evaluated.
SEMINARS IN FETAL & NEONATAL MEDICINE
(2021)
Article
Immunology
K. Takahashi, H. Imaeda, T. Fujimoto, H. Ban, S. Bamba, T. Tsujikawa, M. Sasaki, Y. Fujiyama, A. Andoh
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2013)
Article
Immunology
H. Imaeda, K. Takahashi, T. Fujimoto, E. Kasumi, H. Ban, S. Bamba, H. Sonoda, T. Shimizu, Y. Fujiyama, A. Andoh
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2013)
Article
Gastroenterology & Hepatology
Yusuke Honzawa, Hiroshi Nakase, Masahiro Shiokawa, Takuya Yoshino, Hirotsugu Imaeda, Minoru Matsuura, Yuzo Kodama, Hiroki Ikeuchi, Akira Andoh, Yoshiharu Sakai, Kazuhiro Nagata, Tsutomu Chiba
Article
Nutrition & Dietetics
Tomoki Aomatsu, Hirotsugu Imaeda, Kenichiro Takahashi, Takehide Fujimoto, Eiji Kasumi, Hiromitsu Ban, Shigeki Bamba, Atsushi Yoden, Hiroshi Tamai, Yoshihide Fujiyama, Akira Andoh
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION
(2013)
Article
Gastroenterology & Hepatology
Hirotsugu Imaeda, Kenichiro Takahashi, Takehide Fujimoto, Shigeki Bamba, Tomoyuki Tsujikawa, Masaya Sasaki, Yoshihide Fujiyama, Akira Andoh
JOURNAL OF GASTROENTEROLOGY
(2014)
Article
Gastroenterology & Hepatology
Hirotsugu Imaeda, Shigeki Bamba, Kenichiro Takahashi, Takehide Fujimoto, Hiromitsu Ban, Tomoyuki Tsujikawa, Masaya Sasaki, Yoshihide Fujiyama, Akira Andoh
JOURNAL OF GASTROENTEROLOGY
(2014)
Article
Gastroenterology & Hepatology
Takehide Fujimoto, Hirotsugu Imaeda, Kenichiro Takahashi, Eiji Kasumi, Shigeki Bamba, Yoshihide Fujiyama, Akira Andoh
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2013)
Article
Immunology
Motohiro Nonaka, Hirotsugu Imaeda, Shogo Matsumoto, Bruce Yong Ma, Nobuko Kawasaki, Eiji Mekata, Akira Andoh, Yasuharu Saito, Tohru Tani, Yoshihide Fujiyama, Toshisuke Kawasaki
JOURNAL OF IMMUNOLOGY
(2014)
Article
Pharmacology & Pharmacy
Eiji Kasumi, Yoshie Kuzumaki, Megumi Sadakata, Hiroyuki Kuzuoka, Norifumi Sato
DRUG DISCOVERIES AND THERAPEUTICS
(2019)
Article
Nutrition & Dietetics
Eiji Kasumi, Norifumi Sato
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION
(2019)