Article
Medicine, General & Internal
Giuseppina Campisciano, Nunzia Zanotta, Vincenzo Petix, Manuela Giangreco, Giuseppe Ricci, Gianpaolo Maso, Manola Comar, Francesco De Seta
Summary: The translation discusses the diagnosis of bacterial vaginosis using Nugent score and qPCR, with qPCR proving to be more informative and accurate. Results showed discordance between Gram staining and qPCR in cases of partial BV, with a significant association between STIs and partial BV.
Article
Obstetrics & Gynecology
Pedro Vieira-Baptista, Ana Rita Silva, Mariana Costa, Rita Figueiredo, Conceicao Saldanha, Carlos Sousa
Summary: The study found that Amsel criteria have poor performance in the diagnosis of bacterial vaginosis, while wet mount microscopy performs well and should be considered in the first approach for diagnosis.
INTERNATIONAL JOURNAL OF GYNECOLOGY & OBSTETRICS
(2022)
Article
Obstetrics & Gynecology
Alison C. Roxby, Nelly R. Mugo, Lynda M. Oluoch, Kenneth Tapia, Melody Wang, Stacy Selke, Bhavna Chohan, Murugi Micheni, Laura Sycuro, Tiffany Yuh, Edinah Casmir, Edith Kimani, Steven Gakuo Maina, Catherine Kiptinness, Kenneth Ngure, Anna Wald
Summary: This study describes the prevalence of bacterial vaginosis in Kenyan adolescents and identifies initiation of sexual activity as the strongest risk factor for both prevalent and incident bacterial vaginosis.
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2023)
Article
Obstetrics & Gynecology
Trishna Mohanty, Prakash Prabhakarrao Doke, Sana Rafiq Khuroo
Summary: This meta-analysis aims to confirm the association between bacterial vaginosis and preterm birth. The results of the study indicate a definite association between bacterial vaginosis and preterm birth.
ARCHIVES OF GYNECOLOGY AND OBSTETRICS
(2023)
Article
Immunology
Bridget M. Whitney, Sujatha Srinivasan, Kenneth Tapia, Eric Munene Muriuki, Bhavna H. Chohan, Jacqueline M. Wallis, Congzhou Liu, Brandon L. Guthrie, R. Scott McClelland, Noah G. Hoffman, David N. Fredricks, Alison C. Roxby
Summary: The study found that the use of DMPA-IM and non-hormonal contraception had different effects on the vaginal microbiome composition and diversity in postpartum women. DMPA-IM users saw a decrease in Nugent score, while non-hormonal contraception users showed relative stability in Nugent score but a decrease in alpha diversity, with differences in total bacterial load.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Microbiology
Yisong Li, Ying Wang, Jie Liu
Summary: This study provides the first genome sequence level description of Mobiluncus and sheds light on its virulence/pathogenicity, functional diversification, and evolutionary dynamics. The study suggests that horizontal gene transfer might be an important strategy for species diversification and pathogenicity.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
L. Ruffier d'Epenoux, E. Tessier, A. Guillouzouic, E. Fayoux, C. Bourigault, P. Bemer, S. Corvec
Summary: There is a possibility of introducing a new molecular test in the routine diagnosis of bacterial vaginosis. This study proposes a combination of Nugent scoring and molecular testing for diagnosing bacterial vaginosis, especially for cases with intermediate Nugent scores.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Mohammed Ahmed, Desalegn Admassu, Degu Abate
Summary: This study aimed to determine the prevalence of bacterial vaginosis and associated factors among pregnant women attending antenatal care in Harar town, eastern Ethiopia from May 5 to July 15, 2021. The results showed a high prevalence of bacterial vaginosis, which was significantly associated with a history of sexually transmitted infection, vaginal discharge, multiple sexual partners, and spontaneous abortion.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Microbiology
Victoria Horrocks, Charlotte K. Hind, Matthew E. Wand, Paul-Enguerrand Fady, Joel Chan, Jade C. Hopkins, Georgina L. Houston, Rachel M. Tribe, J. Mark Sutton, A. James Mason
Summary: Bacterial vaginosis (BV) is a common vaginal infection in women of childbearing age, increasing the risk of sexually transmitted infections and preterm delivery. This study investigates the metabolic diversity of BV-associated bacteria and lactobacilli, and the symbiotic relationships between them, to understand the mechanism and approach to risk prediction in BV and/or preterm birth.
Article
Infectious Diseases
Rajshree Bhujel, Shyam Kumar Mishra, Santosh Kumar Yadav, Kesang Diki Bista, Keshab Parajuli
Summary: Amsel's criteria can be used as an adjunct method to Nugent scoring for the diagnosis of BV, with clue cells and positive whiff test in Amsel's criteria showing good match with Nugent's score.
BMC INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Matamela C. Mabugana, Bianca Da Costa Dias, Etienne E. Muller, Tendesayi Kufa, Lindy Gumede, Mahlape P. Mahlangu, Dumisile V. Maseko, Ranmini S. Kularatne
Summary: Bacterial vaginosis (BV) is an imbalance of vaginal microbiota characterized by a shift from Lactobacillus species predominance to a mixture of anaerobes. The performance characteristics of the Allplex TM BV molecular assay were compared with the reference test Nugent score microscopy for vaginal swab specimens from symptomatic South African women. Out of 213 patients, 99 (46.5%) and 132 (62.0%) were diagnosed with BV by Nugent and AllplexTM, respectively. The AllplexTM BV assay showed a sensitivity of 94.9% (95% CI, 88.7%-97.8%) and a specificity of 66.7% (95% CI, 57.6%-74.6%), with an agreement of 79.8% (95% CI, 73.9%-84.7%) (k = 0.60). Assay design can be improved for better specificity by considering the differences in vaginal microbiomes among women of different ethnicities.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2023)
Article
Infectious Diseases
Marjan J. Bruins, Claudy Oliveira dos Santos, Roger A. M. J. Damoiseaux, Gijs J. H. M. Ruijs
Summary: Vulvovaginitis, caused by bacterial vaginosis, Candida infection, and sexually transmitted infections, is a common issue in general practice. Empirical treatment failure often necessitates bacterial culture to detect potentially pathogenic microorganisms for a successful diagnosis and treatment, reducing unnecessary antimicrobial usage and costs. The Nugent score remains the gold standard for BV detection, despite the availability of various molecular tests on the market.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Immunology
Alexander Swidsinski, Vera Loening-Baucke, Sonja Swidsinski, Jack D. Sobel, Yvonne Doerffel, Alexander Guschin
Summary: This study investigated the adhesive and cohesive patterns of different bacteria in vaginal discharge of women diagnosed with bacterial vaginosis. The results showed that the bacteria in vaginal smears were not randomly assembled, but had specific distribution patterns based on their cohesiveness and adherence to epithelial cells. The study also identified two main types of bacterial vaginosis: biofilm vaginosis and bacterial excess vaginosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Immunology
Linda Abou Chacra, Florence Fenollar, Khoudia Diop
Summary: The vaginal microbiome is an important part of the human microbiome, characterized by unique conditions. Increased diversity in the microbiota can lead to dysbiosis such as bacterial vaginosis, with significant health consequences. Diagnosis and treatment methods are evolving, and research on this topic is becoming increasingly important.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Obstetrics & Gynecology
J. Tuzil, B. Filkova, J. Malina, J. Kerestes, T. Dolezal
Summary: The study found that smoking affects the Nugent score in patients but not vaginal pH. Smoking increases the presence of certain bacteria in the vagina while having no significant impact on the proportion of Lactobacillus.
CESKA GYNEKOLOGIE-CZECH GYNAECOLOGY
(2021)
Review
Immunology
Olivia T. Van Gerwen, Zain Aryanpour, John P. Selph, Christina A. Muzny
Summary: This review provides anatomical details of various vaginoplasty procedures, complications associated with these procedures, and an overview of HIV/STI epidemiology among transfeminine individuals who have undergone vaginoplasty.
INTERNATIONAL JOURNAL OF STD & AIDS
(2022)
Article
Multidisciplinary Sciences
Olivia T. Van Gerwen, Erika L. Austin, Andres F. Camino, L. Victoria Odom, Christina A. Muzny
Summary: HIV prevalence is high among transgender women in the Southeastern U.S. However, the uptake of HIV Pre-Exposure Prophylaxis (PrEP) is low among this population. This study aimed to explore the beliefs associated with PrEP among transgender women in the Southeastern U.S. The findings revealed frustration with the conflation of transgender identity and HIV risk, inadequate representation of transgender individuals in PrEP advertising, concerns about interactions between PrEP and hormone therapy, perception that PrEP is meant for cisgender men who have sex with men, and limited access to trans-affirming healthcare. Nuanced messaging is crucial for effectively educating and engaging transgender women in HIV prevention strategies, including PrEP.
Article
Infectious Diseases
Christina A. Muzny, Leandro A. Mena, Rebecca A. Lillis, Norine Schmidt, David H. Martin, Patricia Kissinger
Summary: This study found that multidose therapy was more effective than single-dose therapy for treating Trichomonas vaginalis. Certain clinical factors, such as baseline symptoms and a history of T. vaginalis infection, can impact treatment outcomes. Having a history of T. vaginalis and receiving single-dose metronidazole were independently associated with a positive test-of-cure for T. vaginalis.
SEXUALLY TRANSMITTED DISEASES
(2022)
Article
Immunology
Christina A. Muzny, Jennifer Balkus, Caroline Mitchell, Jack D. Sobel, Kimberly Workowski, Jeanne Marrazzo, Jane R. Schwebke
Summary: This article summarizes recent research on bacterial vaginosis, including global epidemiology, risk factors, sexual transmission, diagnostic tests, and treatment regimens.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Immunology
Patricia J. Kissinger, Charlotte A. Gaydos, Arlene C. Sena, R. Scott McClelland, David Soper, W. Evan Secor, Davey Legendre, Kimberly A. Workowski, Christina A. Muzny
Summary: Trichomonas vaginalis is a common sexually transmitted infection that affects millions of women and men. Health disparities exist, with African Americans being four times more likely to be infected. In women, the infection increases the risk of HIV acquisition, cervical cancer, preterm birth, and other adverse pregnancy outcomes. More research is needed to understand the clinical significance of infection in men. New diagnostic methods have improved testing accuracy and convenience. Rescreening is recommended three months after treatment due to common repeat and persistent infections in women. Antibiotic resistance in T. vaginalis is currently low but needs to be monitored. Treating sexual partners of infected individuals may be necessary. A randomized controlled trial showed that multidose metronidazole reduced the proportion of women with Trichomonas infection compared to single-dose therapy. The preferred treatment for men is still the 2-g single-dose oral metronidazole regimen.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Christina A. Muzny, Olivia T. Van Gerwen, Davey Legendre
Summary: SEC is an effective, well-tolerated, and safe treatment option for patients aged >= 12 years with trichomoniasis. Its single-dose administration makes it a favorable choice, especially in cases where adherence to multi-dose treatment regimens may be low.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2022)
Article
Virology
Francisco Murilo Zerbini, Stuart G. Siddell, Elliot J. Lefkowitz, Arcady R. Mushegian, Evelien M. Adriaenssens, Poliane Alfenas-Zerbini, Donald M. Dempsey, Bas E. Dutilh, Maria Laura Garcia, R. Curtis Hendrickson, Sandra Junglen, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Malgorzata Lobocka, Hanna M. Oksanen, David L. Robertson, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Donald B. Smith, Nobuhiro Suzuki, Koenraad Van Doorslaer, Anne-Mieke Vandamme, Arvind Varsani
Summary: This article reports on changes to virus taxonomy and nomenclature approved by the International Committee on Taxonomy of Viruses (ICTV) in April 2023. All ICTV members were invited to vote on 174 taxonomic proposals and a revision of the ICTV Statutes that had been approved by the ICTV Executive Committee in July 2022. Majority approval was obtained for all proposals and the revised ICTV Statutes. A significant change was the renaming of existing species according to the binomial format and the classification of gene transfer agents (GTAs) as viriforms. In total, one class, seven orders, 31 families, 214 genera, and 858 species were created.
ARCHIVES OF VIROLOGY
(2023)
Review
Microbiology
Christina A. Muzny, Nuno Cerca, Jacob H. Elnaggar, Christopher M. Taylor, Jack D. Sobel, Barbara van der Pol
Summary: Bacterial vaginosis (BV) is a common cause of vaginal discharge in women of reproductive age and is associated with various negative health outcomes, including increased risk of HIV and other STIs, as well as adverse birth outcomes. The exact cause of BV, a dysbiosis of the vaginal microbiota, is still unknown. This mini-review provides an updated overview of the diagnostic tests used for BV in clinical and research settings, focusing on traditional and molecular diagnostics. It highlights the use of molecular diagnostic assays such as 16S rRNA gene sequencing, shotgun metagenomic sequencing, and FISH, as well as multiplex NAATs, in studying the vaginal microbiota and BV pathogenesis.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Medicine, General & Internal
Christina A. Muzny, Olivia T. Van Gerwen, Julia A. Schroeder, Emma Sophia Kay-Duncan, Krishmita Siwakoti, Kristal J. Aaron, Isaac C. Eastlund, Keonte J. Graves, Jacob H. Elnaggar, Ashutosh Tamhane, Dustin Long, Nicholas Van Wagoner, Evelyn Toh, Christopher M. Taylor
Summary: This prospective study aims to investigate changes in the vaginal microbiota over time in transgender men who initiate testosterone therapy. The study will also identify factors associated with bacterial vaginosis and will be disseminated through conferences and journals as well as shared with gender health clinics and community organizations.
Article
Biotechnology & Applied Microbiology
Lucia G. V. Sousa, Carina Almeida, Christina A. Muzny, Nuno Cerca
Summary: Bacterial vaginosis (BV) is a common vaginal infection worldwide. A peptide nucleic acid (PNA) probe targeting Prevotella bivia, a common BV-associated bacteria, was developed, and a multiplex approach for detection of Gardnerella spp., P. bivia, and Fannyhessea vaginae was optimized. The P. bivia PNA probe specifically detected the target species, and the optimized multiplex approach could identify the presence of these three species in multi-species BV biofilms.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Article
Microbiology
Keonte J. Graves, Colin Reily, Hemant K. Tiwari, Vinodh Srinivasasainagendra, William Evan Secor, Jan Novak, Christina A. Muzny
Summary: Through transcriptome analysis, we identified the mechanisms of resistance to 5-nitroimidazoles. RNA sequencing revealed 304 DEGs, with 134 upregulated genes and 170 downregulated genes.
Article
Cell Biology
Evelyn Toh, Yue Xing, Xiang Gao, Stephen J. Jordan, Teresa A. Batteiger, Byron E. Batteiger, Barbara Van Der Pol, Christina A. Muzny, Netsanet Gebregziabher, James A. Williams, Lora J. Fortenberry, J. Dennis Fortenberry, Qunfeng Dong, David E. Nelson
Summary: The origin, composition, and significance of the distal male urethral microbiome are still unknown. However, vaginal microbiome dysbiosis is associated with new sex partners and urogynecological syndromes. This study found that sexual behavior strongly affects the composition of the urethral microbiome, suggesting that urogenital microbiology and sexual behavior are closely interconnected.
CELL REPORTS MEDICINE
(2023)
Correction
Virology
Francisco Murilo Zerbini, Stuart G. Siddell, Elliot J. Lefkowitz, Arcady R. Mushegian, Evelien M. Adriaenssens, Poliane Alfenas-Zerbini, Donald M. Dempsey, Bas E. Dutilh, Maria Laura Garcia, R. Curtis Hendrickson, Sandra Junglen, Mart Krupovic, Jens H. Kuhn, Amy J. Lambert, Malgorzata Lobocka, Hanna M. Oksanen, David L. Robertson, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Donald B. Smith, Nobuhiro Suzuki, Koenraad Van Doorslaer, Anne-Mieke Vandamme, Arvind Varsani
ARCHIVES OF VIROLOGY
(2023)
Letter
Biotechnology & Applied Microbiology
Evelien M. M. Adriaenssens, Simon Roux, J. Rodney Brister, Ilene Karsch-Mizrachi, Jens H. H. Kuhn, Arvind Varsani, Tong Yigang, Alejandro Reyes, Cedric Lood, Elliot J. J. Lefkowitz, Matthew B. B. Sullivan, Robert A. A. Edwards, Peter Simmonds, Luisa Rubino, Sead Sabanadzovic, Mart Krupovic, Bas E. E. Dutilh
NATURE BIOTECHNOLOGY
(2023)
Correction
Biotechnology & Applied Microbiology
Evelien M. M. Adriaenssens, Simon Roux, J. Rodney Brister, Ilene Karsch-Mizrachi, Jens H. Kuhn, Arvind Varsani, Tong Yigang, Alejandro Reyes, Cedric Lood, Elliot J. Lefkowitz, Matthew B. B. Sullivan, Robert A. A. Edwards, Peter Simmonds, Luisa Rubino, Sead Sabanadzovic, Mart Krupovic, Bas E. E. Dutilh
NATURE BIOTECHNOLOGY
(2023)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
