Review
Public, Environmental & Occupational Health
Tobias M. Appel, Natalia Quijano-Martinez, Elsa De La Cadena, Maria F. Mojica, Maria Virginia Villegas
Summary: Species of Raoultella and Klebsiella share many similarities and are practically indistinguishable in clinical microbiology laboratories due to technological limitations. There has been an increase in case reports of human Raoultella infections since the late 2000s, leading some authors to suggest Raoultella spp. as rare and/or emerging pathogens. Further studies are needed to clarify the epidemiology and clinical relevance of human Raoultella infections, as well as the impact of advancements in identification techniques on the reported cases. Raoultella spp. may be more underdiagnosed than considered rare or emerging pathogens.
FRONTIERS IN PUBLIC HEALTH
(2021)
Article
Genetics & Heredity
Aline Cuenod, Daniel Wuethrich, Helena M. B. Seth-Smith, Chantal Ott, Christian Gehringer, Frederic Foucault, Roxanne Mouchet, Ali Kassim, Gunturu Revathi, Deborah R. Vogt, Stefanie von Felten, Stefano Bassetti, Sarah Tschudin-Sutter, Timm Hettich, Gotz Schlotterbeck, Christina Homberger, Carlo Casanova, Jacob Moran-Gilad, Orli Sagi, Belen Rodriguez-Sanchez, Franco Mueller, Martina Aerni, Valeria Gaia, Helke van Dessel, Greetje A. Kampinga, Claudia Mueller, Claudia Daubenberger, Valentin Pflueger, Adrian Egli
Summary: This study analyzed a diverse selection of Klebsiella spp. isolates from various healthcare institutions, identifying group and species-specific differences in antibiotic resistance and pathogenicity. Using species-specific marker masses, eight Klebsiella species can be distinguished using MALDI-TOF MS. Klebsiella oxytoca group isolates were less likely to be resistant to 4th-generation cephalosporins compared to Klebsiella pneumoniae group isolates, and were more associated with invasive infections.
Article
Microbiology
Eva Gato, Ignacio Pedro Constanso, Bruno Kotska Rodino-Janeiro, Paula Guijarro-Sanchez, Tyler Alioto, Manuel Jesus Arroyo, Gema Mendez, Luis Mancera, Marta Gut, Ivo Gut, Miguel Alvarez-Tejado, German Bou, Marina Oviano
Summary: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been used for direct detection of KPC-producing isolates by analyzing the 11,109 Da mass peak representing the P019 protein. The study found the P019 peak prevalence in bla(KPC) K. pneumoniae isolates to be 49.2%, while it was absent in other carbapenemase or noncarbapenemase producers. Variability of P019 presence was observed in different geographical locations within the EuSCAPE collection, indicating a need for reconsideration when applying this technique in a clinical setting.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Eva Gato, Ignacio Pedro Constanso, Ana Candela, Fatima Galan, Bruno Kotska Rodino-Janeiro, Manuel Jesus Arroyo, Gema Mendez, Luis Mancera, Tyler Alioto, Marta Gut, Ivo Gut, Miguel Alvarez-Tejado, Belen Rodriguez-Sanchez, German Bou, Marina Oviano
Summary: The study aimed to standardize an operating procedure and data analysis for identification of CPK using MALDI-TOF MS. Analysis of 162 K. pneumoniae isolates showed that 100% of CPK isolates were correctly identified through a neural network analysis and applying TIC normalization. This demonstrates the feasibility of real-time tracking of CPK using MALDI-TOF MS.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Infectious Diseases
Luz Cuello, Judith Alvarez Otero, Kerryl E. Greenwood-Quaintance, Liang Chen, Blake Hanson, Jinnethe Reyes, Lauren Komarow, Lizhao Ge, Zane D. Lancaster, Garrett G. Gordy, Audrey N. Schuetz, Robin Patel
Summary: The MALDI Biotyper MBT Subtyping Module can detect Klebsiella pneumoniae carbapenemase (KPC) and p019 gene in Klebsiella species with high specificity, but has poor sensitivity in detecting bla(KPC)-positive isolates.
Article
Infectious Diseases
Lukas Hleba, Miroslava Hlebova, Anton Kovacik, Juraj Cubon, Juraj Medo
Summary: KPC-producing bacteria are highly dangerous and antibiotic resistant. Rapid detection of KPC-producing bacteria is crucial in clinical microbiology. The use of MALDI-TOF technology for detecting carbapenemase-producing K. pneumoniae is fast and accurate, but may require verification with other methods.
Article
Microbiology
Iliana Alejandra Cortes-Ortiz, Edgar Mendieta-Condado, Noe Escobar-Escamilla, Julio Cesar Juarez-Gomez, Fabiola Garces-Ayala, Adnan Araiza Rodriguez, Juan Carlos Bravata-Alcantara, Victor Hugo Gutierrez-Munoz, Juan Manuel Bello-Lopez, Jose Ernesto Ramirez-Gonzalez
Summary: Class D beta-lactamases OXA-232 and OXA-48 were found to hydrolyze penicillin, cephalosporins, and carbapenems, limiting therapeutic options for bacteraemia. The study identified Raoultella ornithinolytica carrying OXA 232 and OXA-48 genes, confirming the results of the beta-lactamases assay. This represents the first report of multidrug resistance R. ornithinolytica in Mexico, highlighting the emergence of OXA producer microorganisms as a significant threat.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Microbiology
Yuki Ohama, Yusuke Nomura, Miyuki Mizoguchi, Yoshimi Higurashi, Koh Okamoto, Sohei Harada
Summary: Recent use of bacterial whole-genome sequencing has led to the discovery of new bacterial species and reclassification of taxonomy. Accurate identification methods for bacterial species in clinical microbiology laboratories are limited. This study found that matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry can accurately identify Klebsiella variicola. Accurate identification will help in studying the clinical characteristics of K. variicola.
MICROBIOLOGY SPECTRUM
(2022)
Article
Automation & Control Systems
Alejandro Guerrero-Lopez, Carlos Sevilla-Salcedo, Ana Candela, Marta Hernandez-Garcia, Emilia Cercenado, Pablo M. Olmos, Rafael Canton, Patricia Munoz, Vanessa Gomez-Verdejo, Rosa del Campo, Belen Rodriguez-Sanchez
Summary: A multi-view heterogeneous Bayesian model (KSSHIBA) is proposed for predicting antibiotic resistance using MALDI-TOF MS data and epidemiological differences. This model provides interpretable results and eliminates the need for preprocessing steps by using raw data, reducing the time required for resistance mechanism analysis in microbiological laboratories.
ENGINEERING APPLICATIONS OF ARTIFICIAL INTELLIGENCE
(2023)
Article
Infectious Diseases
Camila Morschbacher Wilhelm, Everton Inamine, Andreza Francisco Martins, Afonso Luis Barth
Summary: Resistance to carbapenems due to the co-production of NDM and ESBL or NDM and KPC is increasing. Combined therapy with aztreonam (ATM) plus ceftazidime/avibactam (CZA) has been recommended. A fast and simple method using MALDI-TOF MS (SynMALDI) was developed to determine the synergism of ATM and CZA.
Article
Infectious Diseases
Jinyu Wang, Cuiping Xia, Yue Wu, Xin Tian, Ke Zhang, Zhongxin Wang
Summary: This study developed RF, SVM, and SVM-K models combined with MALDI-TOF MS to rapidly detect and identify CRKP and CSKP. The results showed that these models achieved good performance in accuracy, sensitivity, specificity, and AUC, with the SVM-K model being the best.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Microbiology
Yunus Emre Ibik, Nebahat Ejder, Elif Sevim, Erva Rakici, Elif Seren Tanriverdi, Aysegul Copur Cicek
Summary: This study evaluated antibiotic resistance and virulence genes, as well as the clonal relationship, of carbapenem-nonsusceptible Klebsiella pneumoniae strains isolated from clinical specimens in a tertiary care hospital in Turkey. The study found various resistance and virulence genes in the strains, with high compatibility between immunochromatographic tests and PCR results. ST101 was identified as the most common clone among the strains.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2023)
Article
Microbiology
Chunxuan Wang, Zhuo Wang, Hsin-Yao Wang, Chia-Ru Chung, Jorng-Tzong Horng, Jang-Jih Lu, Tzong-Yi Lee
Summary: This study utilizes machine learning methods and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to rapidly identify ciprofloxacin-resistant Klebsiella pneumoniae. The models developed show sensitive predictions of antibiotic resistance, with potential impact from suspicious sub-strains.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Emilie Cardot Martin, Claudine Renaux, Emilie Catherinot, Lucie Limousin, Louis Jean Couderc, Marc Vasse
Summary: This study evaluated the new ID-FUNGI plate for mold identification using MALDI Biotyper, and found that IDFP significantly improved species identification compared to traditional Sabouraud agar with gentamicin and chloramphenicol. The results of the study suggest that the new IDFP can enhance mycological diagnostic accuracy and workflow in laboratories.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Immunology
Haoyun Lin, Zhen Hu, Jinsong Wu, Yuemei Lu, Jine Chen, Wenyuan Wu
Summary: In this study, a VITEK mass spectrometry method was developed and validated for the rapid detection of carbapenemase-producing Klebsiella pneumoniae (CPKP). The method showed high accuracy and speed in detecting different types of CPKP strains, making it especially useful for common types of CPKP.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Letter
Microbiology
Arnaud Riat, Rob J. Rentenaar, Adriaan M. van Drongelen, Valerie Barras, Loes C. M. Bertens, Anne L. M. Vlek, Eric Doppenberg, Annemarie J. L. Weersink, Erik Reinders, Bart J. M. Vlaminckx, Natasja Overbeeke, Nathalie D. van Burgel, Niels Peterse, Ron Bosboom, Teun Boekhout, Jacques Schrenzel, Johannes G. Kusters
JOURNAL OF CLINICAL MICROBIOLOGY
(2015)
Article
Microbiology
Valentijn A. Schweitzer, Alje P. van Dam, Putu Yuda Hananta, Rob Schuurman, Johannes G. Kusters, Rob J. Rentenaar
JOURNAL OF CLINICAL MICROBIOLOGY
(2016)
Letter
Microbiology
R. J. Rentenaar, S. M. Kusen, G. M. A. Riemens-van Zetten, M. S. M. van Mourik
JOURNAL OF CLINICAL MICROBIOLOGY
(2018)
Article
Nutrition & Dietetics
E. D. Olthof, R. J. Rentenaar, A. J. M. M. Rijs, G. J. A. Wanten
CLINICAL NUTRITION
(2013)
Editorial Material
Microbiology
Rob J. Rentenaar, Marrigje H. Nabuurs-Franssen, Arjan S. de Jong, Henry A. van Swieten, Patrick D. J. Sturm
JOURNAL OF CLINICAL MICROBIOLOGY
(2014)
Article
Infectious Diseases
Eefje de Jong, Sandra Erkens-Hulshof, Lieven B. J. van der Velden, Andreas Voss, Ron Bosboom, Caspar J. Hodiamont, Peter C. Wever, Rob J. Rentenaar, Patrick D. Sturm
SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES
(2014)
Article
Immunology
Ad C. Fluit, Rob J. Rentenaar, Miquel B. Ekkelenkamp, Tim T. Severs, Annelies M. C. Mavinkurve-Grnothuis, Malbert R. C. Rogers, Marrie C. A. Bruin, Tom F. W. Wolfs
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2019)
Article
Infectious Diseases
Bas T. Franssens, Ad C. Fluit, Rob J. Rentenaar
INFECTIOUS DISEASES
(2019)
Article
Infectious Diseases
Theo van den Broek, Nard G. Janssen, David J. Hetem, Wouter Bekers, Miranda Kamst, Ad C. Fluit, Jakko van Ingen, Johannes G. Kusters, Rob J. Rentenaar
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2019)
Article
Critical Care Medicine
P. Hellebrekers, R. J. Rentenaar, M. A. McNally, F. Hietbrink, R. M. Houwert, L. P. H. Leenen, G. A. M. Govaert
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
(2019)
Letter
Infectious Diseases
M. Heuvelmans, P. D. J. Sturm, R. J. Rentenaar
INFECTIOUS DISEASES
(2019)
Article
Infectious Diseases
Denise van Hout, Axel B. Janssen, Rob J. Rentenaar, Judith P. M. Vlooswijk, C. H. Edwin Boel, Marc J. M. Bonten
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2020)
Article
Orthopedics
Bruce Van Dijk, F. Ruben H. A. Nurmohamed, J. Fred F. Hooning van Duijvenbode, Ewout S. Veltman, Rob J. Rentenaar, Harrie Weinans, H. Charles Vogely, Bart C. H. van der Wal
Summary: This retrospective observational study evaluated the long-term results of the 1- and 2-stage revision procedures for periprosthetic joint infection. The study found no difference in infection control and infection-free survival between the two procedures. Additionally, the study found that an antibiotic-free window in the 2-stage revision did not affect treatment outcomes.
Article
Infectious Diseases
Jonathan Sliepen, Ruth A. Corrigan, Maria Dudareva, Marjan Wouthuyzen-Bakker, Rob J. Rentenaar, Bridget L. Atkins, Geertje A. M. Govaert, Martin A. McNally, Frank F. A. IJpma
Summary: This international, multi-center study evaluated the effectiveness of antibiotic-loaded carriers (ALCs) in patients with fracture-related infections (FRIs), and also assessed whether bacterial resistance to the implanted antibiotics affects their efficacy. The study found that using ALCs in the treatment of FRIs is likely to reduce the risk of infection recurrence. Resistance of cultured microorganisms to the implanted antibiotic was not associated with a higher risk of recurrence.
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
