Article
Microbiology
Mingze Cao, Weiwei Wang, Liwei Zhang, Guanhui Liu, Xuzheng Zhou, Bing Li, Yuxiang Shi, Zhen Zhu, Jiyu Zhang
Summary: This study investigated fluoroquinolone resistance and molecular characteristics of S. dysenteriae 1 isolated from calves. The study identified new sequence types ST228 and ST229, with 76.32% of isolates showing fluoroquinolone resistance and multidrug resistance. Continuous surveillance is necessary to control the increasing emergence of multidrug-resistant isolates.
Article
Food Science & Technology
Zengfeng Zhang, Shoukui He, Xuebin Xu, Jiang Chang, Zeqiang Zhan, Yan Cui, Chunlei Shi, Xianming Shi
Summary: The study revealed an increase in antimicrobial resistance in Salmonella enterica serovar Indiana in China, particularly to ciprofloxacin and ceftriaxone. The detection of drug-resistant strains poses a significant threat to food safety and public health.
Article
Public, Environmental & Occupational Health
Bipasa Kar, Mohan Sharma, Annalisha Peter, Pankaj Chetia, Bijoy Neog, Amrit Borah, Sanghamitra Pati, Debdutta Bhattacharya
Summary: The study analyzed antibiotic resistance patterns in 189 bacterial isolates from Assam Medical College & Hospital, Dibrugarh, revealing high levels of resistance to 3rd generation cephalosporins and fluoroquinolones. ESBL and MBL genes were found to be responsible for resistance, with CTX-M3 and VIM-1 genes being the most dominant. Mutations in QRDR were identified as the primary cause of fluoroquinolone resistance, along with the presence of efflux pump and PMQR. This study, the first detailed study on antibiotic resistance in Northeast India, may help in implementing control measures against emerging antibiotic resistance in hospital and community-based infections.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2021)
Article
Microbiology
Man-Xia Chang, Jin-Fei Zhang, Yin-Huan Sun, Rong-Sheng Li, Xiao-Ling Lin, Ling Yang, Mark A. Webber, Hong-Xia Jiang
Summary: The study analyzed 176 Salmonella isolates from animals with different ciprofloxacin MICs and found that the ParC T57S substitution was common in strains with low ciprofloxacin MICs, while increased MICs depended on the type of GyrA mutation. The presence of PMQR genes provided a route for resistance development in the absence of target-site mutations, and the transfer of plasmid-mediated quinolone resistance genes from plasmids to chromosomes resulted in decreased ciprofloxacin susceptibility and increased fitness. Additionally, overexpression of AcrAB-TolC played a significant role in isolates with slight decreases in susceptibility, with expression being upregulated by MarA more frequently than by RamA.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Lawrence Belotindos, Marvin Villanueva, Joel Miguel, Precious Bwalya, Tetsuya Harada, Ryuji Kawahara, Chie Nakajima, Claro Mingala, Yasuhiko Suzuki
Summary: A high prevalence of E. coli harboring quinolone-resistant determinants was found in food-producing animals and products derived from them in the Philippines, with mutations predominantly in the quinolone-resistance determining regions (QRDRs) of gyrA and parC. Additionally, isolates with mutations in QRDR and/or plasmid-mediated quinolone resistance (PMQR) genes were mostly classified into phylogroup B1 and Enteroaggregative E. coli.
Article
Microbiology
Qing Zhan, Yanlei Xu, Bingjie Wang, Jingyi Yu, Xiaofei Shen, Li Liu, Xingwei Cao, Yinjuan Guo, Fangyou Yu
Summary: The study investigated fluoroquinolone resistance in 149 clinical CRKP isolates associated with bloodstream infections in China from 2015 to 2018. 78.5% of these isolates were resistant to ciprofloxacin, mainly due to mutations in the gyrA and parC genes.
Article
Microbiology
Zohreh Neyestani, Farzad Khademi, Roghayeh Teimourpour, Mojtaba Amani, Mohsen Arzanlou
Summary: This study investigated the prevalence and molecular characteristics of ciprofloxacin resistance in Escherichia coli isolates from various sources in Iran. The study found that 28.03% of the isolates were resistant to ciprofloxacin, with the highest resistance observed in isolates from clinical specimens. The study also identified mutations in several genes and the presence of plasmid-mediated quinolone resistance (PMQR) genes among the ciprofloxacin-resistant isolates.
Article
Chemistry, Medicinal
Ashish Kothari, Neeraj Jain, Shyam Kishor Kumar, Ankur Kumar, Karanvir Kaushal, Satinder Kaur, Atul Pandey, Amit Gaurav, Balram Ji Omar
Summary: The rise in multiple-drug-resistant phenotypes in Gram-negative pathogens, particularly Pseudomonas aeruginosa, has become a major public health crisis. This study investigated the frequency of multidrug-resistant and extensively drug-resistant phenotypes in a specialized tertiary care hospital in north India. Approximately 60% of P. aeruginosa strains displayed multidrug-resistant phenotype, with higher antibiotic resistance frequency observed in the emergency department. Plasmids carrying quinolone-resistance determinants were identified as major drivers for fluoroquinolone resistance. Combinatorial antibiotic therapy using colistin and ciprofloxacin was found to be an effective treatment option for infections caused by MDR and XDR P. aeruginosa.
Article
Microbiology
Jiacui Lai, Hao Mu, Bingqian Zhou, Jiawei He, Xiangning Cheng, Yujie Gan, Meiyuan Zhao, Mengqi Xie, Yang Zhang, Ying He, Yujiao Yang, Jian Wang, Haoju Wang, Honglei Ding
Summary: This study aimed to investigate the epidemiological characteristics and antimicrobial resistance of Salmonella from livestock and poultry in Chongqing municipality, China. The results showed that Salmonella strains from different animal sources exhibited high resistance to multiple antibiotics and showed multidrug resistance phenotypes. Many isolates also carried beta-lactamase genes and plasmid-mediated quinolone resistance genes. To ensure public health, rational use and strict control strategies for antimicrobials in animal husbandry and treatment are necessary.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Environmental Sciences
Jianbin Zhao, Chaonan Zhang, Yanbin Xu, Xinyan Li, Xiaojun Lin, Zitao Lin, Tiangang Luan
Summary: Multidrug-resistant bacteria pose a serious threat to disease treatment and recovery, but their potential toxicity to animal development is not entirely clear. In this study, the impact of multidrug-resistant Shigella on the intestinal health of Xenopus tropicalis was investigated. The intake of Shigella led to neutral mucus secretion, inhibited intestinal development and weight gain, and caused intestinal lesions. Additionally, the presence of Shigella altered the expression of cytokines and antioxidant enzyme activities in the gut, and increased the abundance of antibiotic resistance genes.
ENVIRONMENTAL POLLUTION
(2023)
Article
Microbiology
Melisa Willby, Paige Chopra, Darrin Lemmer, Katherine Klein, Tracy L. Dalton, David M. Engelthaler, J. Peter Cegielski, James E. Posey
Summary: Fluoroquinolones play a crucial role in the treatment of multidrug-resistant tuberculosis. Mutations in the gyrA gene are associated with different levels of resistance. Understanding the mechanisms behind FQ resistance can lead to strategies for improved treatment success.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Rasel Khan Manik, Zimam Mahmud, Israt Dilruba Mishu, Md Sourav Hossen, Zakir Hossain Howlader, A. H. M. Nurun Nabi
Summary: This study provides important insights into the prevalence and mechanisms of antibiotic resistance in hospital wastewater isolates. The results showed that resistance to multiple antibiotic classes was common among the isolates, and the most common resistance genes were blaTEM, blaCTX-M1, and blaNDM. Polymyxin was found to be the most effective antibiotic against wastewater isolates.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Immunology
Reo Onishi, Katsumi Shigemura, Kayo Osawa, Young-Min Yang, Koki Maeda, Hiroshi Tanimoto, Mitsuki Kado, Shiuh-Bin Fang, Shian-Ying Sung, Takayuki Miyara, Masato Fujisawa
Summary: This study investigated quinolone resistance among ESBL-producing K. pneumoniae isolated from UTI patients in Indonesia. The results showed that QRDR mutations clearly had a greater effect on resistance against quinolones than the presence of PMQR genes, with 84.8% of strains being resistant to at least one quinolone.
PATHOGENS AND DISEASE
(2022)
Article
Microbiology
Guoping Cai, Yan Wang
Summary: This study investigated the molecular patterns of clinically important fluoroquinolone resistance (CI-FR) in multidrug-resistant Klebsiella pneumoniae (MDR-KP) isolates in a tertiary hospital in Shanghai, PR China. The results showed that mutations in quinolone resistance-determining region (QRDR) and the acquisition of plasmid-mediated quinolone resistance (PMQR) genes played a role in the development of CI-FR. High-risk CRKP clones responsible for nosocomial outbreaks were also identified.
JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Article
Immunology
Radhika Gharpure, Cindy R. Friedman, Veronica Fialkowski, Jennifer P. Collins, Jonathan Strysko, Zachary A. Marsh, Jessica C. Chen, Elizabeth H. Meservey, Azizat A. Adediran, Morgan N. Schroeder, Ashutosh Wadhwa, Kathleen E. Fullerton, Louise Francois Watkins
Summary: A study investigated a multi-drug resistant Shigella outbreak where 4 patients were treated with azithromycin, all of whom experienced treatment failure, with 2 also showing microbiologic failure; 2 patients who received ciprofloxacin treatment also failed. The findings underscore the importance of identifying alternative treatment options for resistant strains.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)