Journal
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volume 73, Issue 4, Pages 338-342Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2012.05.011
Keywords
Echinocandins; Antifungal susceptibility; Impaired killing; Serum-based susceptibility testing
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Funding
- MSD
- Pfizer
- Richter Gedeon Pharma PhD scholarship
- Bolyai Research Scholarship of the Hungarian Academy of Sciences
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Micafungin activity was determined against 24 wild-type clinical isolates and 5 American Type Culture Collection strains belonging to 8 Candida species in RPMI-1640 with and without 50% serum using broth microdilution and time-kill methodology. MIC values increased from 4- to 128-folds in 50% serum for all Candida species. Micafungin was not fungicidal against C albicans, C. tropicalis, and against 2 of 3 degrees C. metapsilosis at >= 0.25, 1, and 1 mu g/mL, respectively, after 48 h with 50% serum, showing good fungistatic activity. Fungicidal activity at >= 2, 4, and 32 mu g/mL was noticed against C. glabrata, C inconspicua, and C krusei isolates, respectively. Micafungin at 8-32 mu g/mL showed fungistatic activity against C. parapsilosis and C. orthopsilosis. Serum decreased the in vitro activity of micafungin. With serum binding of echinocandins taken into account, safely fungistatic or fungicidal concentrations seem to require elevated doses against some Candida species, including C. parapsilosis, C. orthopsilosis, and C. krusei. (C) 2012 Elsevier Inc. All rights reserved.
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