Article
Infectious Diseases
Lillie M. Powell, Soo Jeon Choi, Breanna L. Haught, Ryan Demkowicz, P. Rocco LaSala, Slawomir Lukomski
Summary: This study in West Virginia found that the emm92 type of Group A Streptococcus was associated with infections in adult intravenous drug users, but not with pediatric pharyngitis. All emm92 strains were resistant to erythromycin and clindamycin.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Rui Yu, Yindi Xu, Stefan Schwarz, Yanhong Shang, Xuezhen Yuan, Yue Zhang, Dexi Li, Xiang-Dang Du
Summary: This study investigated the presence and location of erm(T) in clinical Streptococcus suis isolates and explored the transmission ability and fitness cost of erm(T)-carrying mobile genetic elements. The results showed that erm(T) was detected on a plasmid and an integrative and conjugative element (ICE) in S. suis isolates, which were transmissible by transformation. The erm(T)-carrying plasmid and ICE posed a fitness cost to the host S. suis isolate.
MICROBIOLOGY SPECTRUM
(2022)
Article
Medical Laboratory Technology
Seong Jin Choi, Jieun Kang, Young Uh
Summary: The colonization rate of group B Streptococcus (GBS) among pregnant Korean women has increased to levels observed in Western countries, with a certain level of antibiotic resistance present. Periodic studies in multiple centers, including primary clinics, are necessary to accurately evaluate GBS epidemiology among pregnant Korean women.
ANNALS OF LABORATORY MEDICINE
(2021)
Article
Immunology
Lillie M. Powell, Soo Jeon Choi, Chloe E. Chipman, Megan E. Grund, P. Rocco LaSala, Slawomir Lukomski
Summary: Clindamycin and beta-lactam antibiotics are commonly used for the treatment of invasive group A Streptococcus (iGAS) infection. However, strains displaying MLSB phenotypes may be resistant to these regimens. A study in West Virginia, USA, investigated 76 iGAS isolates and found a high prevalence of simultaneous resistance to erythromycin and clindamycin, particularly in certain emm types. Macrolide resistance was mediated by different genes in different emm types. The study also observed a shift in emm type distribution compared to historical reports.
EMERGING INFECTIOUS DISEASES
(2023)
Letter
Infectious Diseases
Bryan P. White, Emily A. Siegrist
Summary: The study mainly focuses on clinical forms of early-stage neurosyphilis and is not applicable to asymptomatic or late-stage neurosyphilis. There is an issue of unbalanced baseline characteristics in the study, which may indicate a potential bias.
LANCET INFECTIOUS DISEASES
(2021)
Article
Medicine, General & Internal
Hye Gyung Bae, Jungmi Hong, Young-Jin Kim, Kyoung-Ryul Lee, Kyungwon Lee, Seong Jin Choi, Young Uh
Summary: The prevalence of Group B Streptococcus (GBS) colonization in pregnant Korean women is increasing. This nationwide survey aimed to analyze regional colonization rates and antimicrobial susceptibility for GBS in pregnant Korean women. The study found that the overall nationwide GBS colonization rate was 10.6%, with provincial rates ranging from 9.6% to 13.2%. The study also identified high resistance rates against clindamycin.
YONSEI MEDICAL JOURNAL
(2022)
Article
Microbiology
M. Belen Cubria, Luis Alberto Vega, William C. Shropshire, Misu A. Sanson, Brittany J. Shah, Shrijana Regmi, Marcia Rench, Carol J. Baker, Anthony R. Flores
Summary: Group B Streptococcus (GBS) is a major cause of invasive bacterial disease in neonates and young infants, and it also affects adults and the elderly. GBS isolates are becoming resistant to second line antibiotics. This study examined the association of antimicrobial resistance with the emergence of invasive GBS in adults by analyzing whole-genome sequencing data from strains isolated in the United States and Canada over a 45-year period. The findings show a temporal association between increased macrolide resistance and the emergence of GBS subpopulations causing invasive disease in adults and infants. The presence of macrolide resistance genes in mobile genetic elements contributes to GBS virulence. This research provides a foundation for further investigation into the genetic features contributing to the prevalence and pathogenesis of GBS in adult invasive disease.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Immunology
Ching-Hui Wang, Wan-Ju Kung, Chien-Hung Lee, Cheng-Fa Lee, Chia-Ling Kao, Hui-Chi Chen, Ting-Wen Hsu, Ching-Chiang Lin
Summary: The colonization rate of Group B streptococcus (GBS) remains high among pregnant women, with an increasing antimicrobial resistance. While the implementation of intrapartum antibiotic prophylaxis (IAP) has led to a relatively low incidence of invasive GBS diseases, GBS colonization remains a concern.
Article
Infectious Diseases
Pilar Villalon, Marta Barcena, Maria Jose Medina-Pascual, Noelia Garrido, Silvia Pino-Rosa, Gema Carrasco, Sylvia Valdezate
Summary: This study reported on the antimicrobial resistance of invasive Streptococcus pyogenes in Spain from 2007 to 2020. Different methods were used to identify the resistance genotypes and phenotypes, and eight main resistant clones were identified. The resistance rates of tetracycline, erythromycin, and clindamycin decreased during the study period, influenced by temporal variations in the proportion of resistant clones.
Article
Microbiology
Fen Gao, Lihong Tu, Mingliang Chen, Hongyou Chen, Xi Zhang, Yuan Zhuang, Jiayuan Luo, Min Chen
Summary: The study investigated the prevalence and antimicrobial resistance profiles of Campylobacter strains collected in Shanghai from 2012 to 2019. The results showed high levels of resistance to ciprofloxacin, nalidixic acid, and tetracycline. Genotyping and resistance analysis of erythromycin-resistant isolates revealed a potential zoonotic transmission of erm(B) in Campylobacter. Continuous surveillance of erythromycin resistance dissemination is crucial for maintaining the effectiveness of antimicrobial therapy.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Sonsiray Alvarez Narvaez, Ingrid Fernandez, Nikita V. Patel, Susan Sanchez
Summary: In this study, a multiplex qPCR diagnostic tool was developed for the simultaneous detection of R. equi and related antibiotic resistance genes in equine respiratory samples. The tool showed high sensitivity and specificity, and had good performance in in-silico analysis, demonstrating its potential to improve the speed of R. equi infection diagnosis and treatment selection.
Article
Cell Biology
Kristin Jahn, Patience Shumba, Phoenicia Quach, Mathias Musken, Jan Wesche, Andreas Greinacher, Lakshmi Rajagopal, Sven Hammerschmidt, Nikolai Siemens
Summary: This study investigates the effects of GBS pigment on human platelets and finds that the pigment can induce platelet activation and necrotic cell death.
Article
Medicine, General & Internal
Niluka Dilrukshi, Jananie Kottahachchi, Thushari Dissanayake, Neluka Fernando
Summary: This study assessed the antibiotic susceptibility and characterized antibiotic resistance genes of GBS isolates from selected tertiary care hospitals in Western province, Sri Lanka. GBS colonization rate in the study sample was 25.7%, with all isolates being sensitive to penicillin. The most prevalent resistance genotype was ermTR.
MEDICAL PRINCIPLES AND PRACTICE
(2023)
Article
Infectious Diseases
Weidong Chen, Yuanchun Huang, Xiaoyang Jiao, Jiahui Ye, Yanling Lin, Fen Yao
Summary: This study investigated the effect of combining linezolid with erythromycin on erythromycin-resistant enterococci. Checkerboard techniques showed a synergistic effect between erythromycin and linezolid in 12 out of 14 isolates. The study also found that linezolid treatment suppressed erm(B)-mediated rRNA dimethylation at A2071 and led to loss of the erm(B) gene.
MICROBIAL DRUG RESISTANCE
(2023)
Article
Veterinary Sciences
Xing-Yun Li, Rui Yu, Chunyan Xu, Yanhong Shang, Dexi Li, Xiang-Dang Du
Summary: The study aimed to determine the mobile genetic elements involved in the horizontal transfer of erythromycin resistance in Enterococcus faecalis and investigate its transmission ability in heterologous hosts. It was found that a plasmid carrying the erm(T) gene could be transferred to different bacteria and conferred resistance to erythromycin and clindamycin.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)