Article
Infectious Diseases
M. Cristina Vazquez Guillamet, Christopher Damulira, Andrew Atkinson, Victoria J. Fraser, Scott Micek, Marin H. Kollef
Summary: The combination of aminoglycosides and β-lactams is more effective in reducing the incidence of infections caused by multidrug-resistant Gram-negative bacilli compared to β-lactams alone. This retrospective cohort study analyzed septic patients treated between 2010 and 2017, and found that the addition of aminoglycosides to β-lactams significantly decreased the risk of subsequent infections.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Infectious Diseases
Mariam Rima, Saoussen Oueslati, Laura Dabos, Dina Daaboul, Hassan Mallat, Elie Bou Raad, Marcel Achkar, Osman Mawlawi, Sandrine Bernabeu, Remy A. Bonnin, Delphine Girlich, Marwan Osman, Monzer Hamze, Thierry Naas
Summary: This study investigated the prevalence of carbapenem-resistant Gram-negative bacilli (CR-GNB) in three hospitals in northern Lebanon between 2015 and 2019. The findings highlighted a concerning situation of carbapenem resistance and emphasized the need for effective registration systems in healthcare centers to limit resistance spread.
Article
Biochemistry & Molecular Biology
Malgorzata Brauncajs, Filip Bielec, Anna Macieja, Dorota Pastuszak-Lewandoska
Summary: Gram-negative fermenting and non-fermenting bacteria, especially those that produce carbapenemases, are important etiological factors of nosocomial and community infections. New antibiotics and treatment regimens offer hope against these infections.
Article
Microbiology
Ting Shi, Liangyi Xie
Summary: This study investigated the antimicrobial resistance rates of gram-negative bacilli isolated from clinical settings. It was found that Acinetobacter baumannii and Klebsiella pneumoniae showed a rapid increase in resistance, while Escherichia coli had a relatively low and stable resistance rate. Gram-negative bacilli in the ICU department demonstrated higher resistance rates compared to the non-ICU department, with significant differences.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Malgorzata Brauncajs, Filip Bielec, Anna Macieja, Dorota Pastuszak-Lewandoska
Summary: Eravacycline is a novel antibiotic that can effectively treat carbapenem-resistant strains, including multi-drug-resistant bacteria. However, its use is currently limited to treating complicated intra-abdominal infections.
Article
Dentistry, Oral Surgery & Medicine
Lais Christina Pontes Espindola, Renata Cristina Picao, Stella Maria Casas Novas Mancano, Renata Martins do Souto, Ana Paula Vieira Colombo
Summary: A high prevalence and diversity of Gram-negative bacilli, with low susceptibility to beta-lactams, were observed in the subgingival microbiota associated with periodontitis.
JOURNAL OF PERIODONTOLOGY
(2022)
Article
Infectious Diseases
Ayan Ali Ragueh, Mohamed Houmed Aboubaker, Sitani Idriss Mohamed, Jean-Marc Rolain, Seydina M. Diene
Summary: This study investigated the resistance rate to carbapenems among multidrug-resistant Gram-negative bacteria (MDR-GNB) in Djibouti and characterized their resistance mechanisms. The results showed that out of 256 isolates, 20 were resistant to carbapenems. Carbapenemase genes such as bla(OXA-48) and bla(NDM-5) were identified in the isolates.
Review
Infectious Diseases
Andrew Henderson, Evan Bursle, Adam Stewart, Patrick N. A. Harris, David Paterson, Mark D. Chatfield, Mical Paul, Yaakov Dickstein, Jesus Rodriguez-Bano, John D. Turnidge, Gunnar Kahlmeter
Summary: This study analyzed 32 randomized controlled trials and found that most trials conducted local AST, but did not report quality control, with a common practice of post-hoc referral to reference laboratories for AST. When comparing patients with infections caused by susceptible and non-susceptible pathogens in different trial groups, some antimicrobials showed better patient outcomes in cases of higher susceptibility, but there were also exceptions.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Microbiology
Carmen Antonia Sanches Ito, Larissa Bail, Lavinia Nery Villa Stangler Arend, Kleber Oliveira Silva, Simone Sebold Michelotto, Keite da Silva Nogueira, Felipe Francisco Tuon
Summary: The study evaluated the performance of a new MicroScan Neg multidrug-resistant MIC 1 (NMR1) panel for clinical carbapenem-nonsusceptible Gram-negative bacilli isolates, demonstrating high reliability in detecting ceftazidime-avibactam and ceftolozane-tazobactam. The panel is a viable option for clinical applications, especially for KPC-producing Enterobacterales and carbapenem-nonsusceptible P. aeruginosa.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Swapnil Prakash Doijad, Nicolas Gisch, Renate Frantz, Bajarang Vasant Kumbhar, Jane Falgenhauer, Can Imirzalioglu, Linda Falgenhauer, Alexander Mischnik, Jan Rupp, Michael Behnke, Michael Buhl, Simone Eisenbeis, Petra Gastmeier, Hanna Goelz, Georg Alexander Haecker, Nadja Kaeding, Winfried Kern, Axel Kola, Evelyn Kramme, Silke Peter, Anna M. Rohde, Harald Seifert, Evelina Tacconelli, Maria J. G. T. Vehreschild, Sarah Walker, Janine Zweigner, Dominik Schwudke, Trinad Chakraborty
Summary: A three-year study in Germany and a global analysis of isolates identified Enterobacter xiangfangensis as the most common species (65.5%) within the Enterobacter cloacae complex (ECC). The study revealed widespread resistance to colistin, with the mobile colistin resistance (mcr)-9 gene detected in 19.2% of all isolates. The resistance and heteroresistance properties were found to be dependent on the arnBCADTEF gene cassette, whose products catalyze transfer of L-Ara4N to lipid A.
NATURE COMMUNICATIONS
(2023)
Article
Infectious Diseases
Beatriz Arns, Jaqueline Driemeyer C. Horvath, Gabriela Soares Rech, Guilhermo Prates Sesin, Crepin Aziz Jose Oluwafoumi Agani, Bruna Silveira da Rosa, Tiago Marcon dos Santos, Liliane Spencer Bittencourt Brochier, Alexandre Biasi Cavalcanti, Bruno Martins Tomazini, Adriano Jose Pereira, Viviane Cordeiro Veiga, Giovana Marssola Nascimento, Andre C. Kalil, Alexandre P. Zavascki
Summary: The OPTIMISE trial is a randomized controlled trial designed to assess the duration of antimicrobial therapy in patients with severe infections caused by multidrug-resistant Gram-negative bacteria (MDR-GNB).
INFECTIOUS DISEASES AND THERAPY
(2023)
Article
Microbiology
Alecks Megxel S. Abordo, Mark B. Carascal, Roland Remenyi, Doralyn S. Dalisay, Jonel P. Saludes
Summary: In the clinically isolated Gram-negative bacilli in the Philippines, the co-occurrence of multiple β-lactamases, especially ESBL and metallo-β-lactamases, was observed. Through genetic analysis and phenotypic characterization, the presence of multi-class β-lactamases and cryptic metallo-β-lactamases in these resistant bacteria was revealed.
Article
Multidisciplinary Sciences
Tanshi Mehrotra, Dipasri Konar, Agila Kumari Pragasam, Shakti Kumar, Pradipta Jana, Prabhakar Babele, Deepjyoti Paul, Ayushi Purohit, Subhash Tanwar, Susmita Bakshi, Santanu Das, Jyoti Verma, Daizee Talukdar, Lekshmi Narendrakumar, Akanksha Kothidar, Sonali Porey Karmakar, Susmita Chaudhuri, Sujoy Pal, Kajal Jain, Chittur V. Srikanth, M. Jeeva Sankar, Krishnamohan Atmakuri, Ramesh Agarwal, Rajni Gaind, Mamatha Ballal, Nagamani Kammili, Rupak K. Bhadra, Thandavarayan Ramamurthy, G. Balakrish Nair, Bhabatosh Das
Summary: Microbes evolve rapidly through mutations and acquisition of mobile genetic elements (MGEs) associated with fitness traits like antimicrobial resistance (AMR). A multicentric study in India decoded genome sequences of bacterial pathogens causing sepsis, urinary tract infections, and respiratory infections. The study identified pathogen-specific AMR genes (ARGs) and MGEs, and differential abundance of resistance-associated proteins in the presence and absence of antibiotics, providing valuable insights for AMR diagnostics and antimicrobial therapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Infectious Diseases
Viveka Nordberg, Aina Iversen, Annika Tidell, Karolina Ininbergs, Christian G. Giske, Lars Naver
Summary: This study in Stockholm, Sweden, found lower incidence of GNB sepsis in neonates compared to previous reports and low occurrence of antibiotic resistance. The in-hospital mortality and 5-day case fatality rate were higher in GNB late-onset sepsis compared to uninfected controls but not in comparison to suspected sepsis.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Faduma Nur Adan, Mohamed Osman Omar Jeele, Nasteho Mohamed Sheikh Omar
Summary: This study analyzes the epidemiology of MDR-NFGNB among HAP patients in a tertiary care hospital in Somalia. The prevalence rate of NFGNB in HAP patients was 8%, with higher rates in men and the elderly. Acinetobacter baumannii and Stenotrophomonas maltophilia exhibited the highest antibiotic resistance rate.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Immunology
Aaron C. Miller, Logan M. Harris, Joseph E. Cavanaugh, Mahmoud Abou Alaiwa, David A. Stoltz, Douglas B. Hornick, Philip M. Polgreen
Summary: Infection-related visits and antibiotic use decreased significantly in patients with cystic fibrosis (CF) after initiating treatment with elexacaftor-tezacaftor-ivacaftor. This nonantimicrobial therapy appears to have a substantial impact on respiratory infections and antimicrobial use in CF patients.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Public, Environmental & Occupational Health
Thomas R. Talbot, Susan E. Beekmann, Hilary M. Babcock, Philip M. Polgreen
Summary: This study examined the exemption policies and practices for healthcare personnel (HCP) COVID-19 vaccine requirements. The results showed that many healthcare institutions allow different types of exemptions, but there is significant variation in the types of exemptions allowed and how the exemption programs are structured.
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
(2022)
Article
Multidisciplinary Sciences
Andrew B. Janowski, Philip M. Polgreen, Susan E. Beekmann, Jason G. Newland
Summary: The perception of the transmission risks of SARS-CoV-2 in social and educational settings by US healthcare providers have different perspectives. High-risk activities identified include spending time in a bar, eating at a restaurant, and attending an indoor sporting event. Compared to students, teachers and pediatric providers have a lower perception of transmission risks.
Article
Immunology
Kapil K. Saharia, Shweta Anjan, Judy Streit, Susan E. Beekmann, Philip M. Polgreen, Matthew Kuehnert, Dorry L. Segev, John W. Baddley, Rachel A. Miller
Summary: Solid organ transplant recipients (SOTR) who have breakthrough infections after COVID-19 vaccination can develop severe and even critical illness. Two doses of COVID-19 vaccine may be insufficient to protect SOTR against severe disease and mortality.
TRANSPLANT INFECTIOUS DISEASE
(2022)
Article
Medicine, General & Internal
Manish Suneja, Susan E. Beekmann, Gurpreet Dhaliwal, Aaron C. Miller, Philip M. Polgreen
Summary: This study investigated the diagnostic delays in infectious diseases and found that the failure to consider the diagnosis initially and not ordering appropriate tests were the two most important contributing factors. Unusual clinical presentations and not consulting infectious diseases physicians early enough were also reported as causes of delays.
Article
Public, Environmental & Occupational Health
Michael J. Durkin, Jason Lake, Philip M. Polgreen, Susan E. Beekmann, Adam L. Hersh, Jason G. Newland
Summary: A survey of adult infectious diseases physicians revealed concerns about the unintended consequences of antimicrobial stewardship programs, such as disagreement with colleagues, provider autonomy, and remote recommendations. Non-ASP physicians expressed more concern about the focus on costs, provider efficiency, and unintended consequences of ASP guidance.
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
(2023)
Article
Urology & Nephrology
Jason M. Sandberg, Hayden L. Warner, Kevin J. Flynn, Shawn M. Sexton, Hanh Td Pham, Blaize W. Kandler, Phillip M. Polgreen, Bradley A. Erickson
Summary: This study evaluated the efficacy of early primary wound closure in the treatment of early necrotizing soft-tissue infections of the genitalia. The results showed that early closure can decrease the need for skin grafting and reduce wound convalescence time.
Article
Microbiology
Aaron C. Miller, Alan T. Arakkal, Scott H. Koeneman, Joseph E. Cavanaugh, George R. Thompson, John W. Baddley, Philip M. Polgreen
Summary: The study analyzed diagnostic delays associated with histoplasmosis using IBM Marketscan data, revealing that patients experienced multiple visits and missed opportunities for diagnosis before being diagnosed. The average delay in diagnosis was 39.5 days, with patients who had prior antibiotic use, history of other pulmonary diseases, and visited on weekends being more likely to experience delays. New diagnostic approaches for histoplasmosis are needed.
Article
Public, Environmental & Occupational Health
Jason G. Lake, Michael J. Durkin, Philip M. Polgreen, Susan E. Beekmann, Adam L. Hersh, Jason G. Newland
Summary: Pediatric antimicrobial stewardship programs (ASPs) have been found to improve antibiotic use for hospitalized children, but there is a lack of data on infectious disease physicians' opinions of ASPs. A survey was conducted to assess the perceptions of pediatric infectious disease physicians, both ASP and non-ASP, regarding ASP practices and outcomes.
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
(2023)
Article
Immunology
Patrick B. Mazi, Sandra R. Arnold, John W. Baddley, Nathan C. Bahr, Susan E. Beekmann, Todd P. McCarty, Philip M. Polgreen, Adriana M. Rauseo, Andrej Spec
Summary: Histoplasmosis is still common in the Mississippi and Ohio river valleys, but there have been reported cases outside these endemic areas. The variability of management strategy is higher for immunocompromised patients due to the lack of strong recommendations. Most infectious disease physicians follow the guidelines recommending itraconazole for histoplasmosis treatment, but there is no consensus for immunocompromised patients.
OPEN FORUM INFECTIOUS DISEASES
(2022)
Article
Urology & Nephrology
Bradley A. A. Erickson, Aaron C. C. Miller, Hayden L. L. Warner, Justin N. N. Drobish, Scott H. H. Koeneman, Joseph E. E. Cavanaugh, Philip M. M. Polgreen
Summary: The purpose of this study was to investigate health care utilization patterns and identify risk factors associated with potential diagnostic delay leading up to the diagnosis of necrotizing soft tissue infections of the genitalia. The study found that nearly 50% of insured patients who undergo surgery for or die from this condition will present to a medical provider with symptomatically similar diagnoses suggestive of early disease development, and many of these visits likely represent diagnostic delay.
JOURNAL OF UROLOGY
(2022)
Article
Medicine, General & Internal
Jacob E. Simmering, Linnea A. Polgreen, David A. Talan, Joseph E. Cavanaugh, Philip M. Polgreen
Summary: This study observed seasonality in the incidence of appendicitis and found an association between increased incidence and warmer weather.
Article
Medicine, General & Internal
Aaron C. Miller, Alan T. Arakkal, Scott H. Koeneman, Joseph E. Cavanaugh, Philip M. Polgreen
Summary: This study uses machine learning approaches to mine administrative data sources and recommend conditions for consideration in studying diagnostic delays. By analyzing initial symptoms and diagnosis codes, the approach successfully identifies clinically-plausible conditions and discovers more possible diagnostic delays in tuberculosis and appendicitis.
Article
Public, Environmental & Occupational Health
Janet B. Glowicz, Emily Landon, Emily E. Sickbert-Bennett, Allison E. Aiello, Karen deKay, Karen K. Hoffmann, Lisa Maragakis, Russell N. Olmsted, Philip M. Polgreen, Polly A. Trexler, Margaret A. VanAmringe, Amber R. Wood, Deborah Yokoe, Katherine D. Ellingson
Summary: The purpose of this document is to provide practical recommendations to acute-care hospitals for preventing healthcare-associated infections through hand hygiene. It updates the previous version published in 2014 and is sponsored by the Society for Healthcare Epidemiology (SHEA) in collaboration with other organizations. This expert guidance document has major contributions from representatives of various organizations and societies.
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Alan T. Arakkal, Linnea A. Polgreen, Cole G. Chapman, Jacob E. Simmering, Joseph E. Cavanaugh, Philip M. Polgreen, Aaron C. Miller
Summary: The presence of opioids in a household significantly increases the risk of overdose among other family members who were not prescribed an opioid. Higher levels of opioid strength and quantity are associated with increased levels of risk. Risk estimates may reflect accidental poisonings among younger family members.
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
