Article
Infectious Diseases
Stefan Monecke, Patrick Eberechi Akpaka, Margaret R. Smith, Chandrashekhar G. Unakal, Camille-Ann Thoms Rodriguez, Khalil Ashraph, Elke Mueller, Sascha D. Braun, Celia Diezel, Martin Reinicke, Ralf Ehricht
Summary: The aim of this study was to comprehensively characterise S. aureus strains from Trinidad and Tobago, and Jamaica. A total of 101 isolates were collected, mainly from patients with skin and soft tissue infections. The isolates were characterised using DNA microarray and the production of Panton-Valentine leukocidin (PVL) was examined. The study found high rates of PVL genes in methicillin-susceptible S. aureus (MSSA) isolates, with a local CC8-MSSA clone and CC152-MSSA being the main contributors. Ten MRSA isolates were identified, including the pandemic ST239-MRSA-III strain and five isolates with a composite SCCmec element not observed elsewhere.
Article
Agriculture, Dairy & Animal Science
Nadia Safia Chenouf, Olouwafemi Mistourah Mama, Chafik Redha Messai, Laura Ruiz-Ripa, Rosa Fernandez-Fernandez, Isabel Carvalho, Abdelghani Zitouni, Ahcene Hakem, Carmen Torres
Summary: This study investigated antimicrobial resistance and virulence factors in Staphylococcus aureus and coagulase-negative staphylococci in unpasteurized milk in Djelfa, Algeria. The results showed contamination of milk samples with these bacteria, with some strains exhibiting resistance to multiple drugs and carrying various resistance genes.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Microbiology
Mohamed Shohayeb, Tarek El-Banna, Lamis E. Elsawy, Maisra M. El-Bouseary
Summary: This study evaluated the prevalence of PVL genes as a marker for CA-MRSA at tertiary hospitals in Mansoura, Dakahlia, Egypt. The results showed that PVL genes were generally highly prevalent among mecA-positive MRSA isolates, whether they are CA-MRSA, HA-MRSA, or HCW isolates. Therefore, PVL is not a valid marker for CA-MRSA in this region.
Article
Multidisciplinary Sciences
Frederike Waldeck, Salome N. Seiffert, Susanne Manser, Danuta Zemp, Angela Walt, Christoph Berger, Werner C. Albrich, Matthias Schlegel, Tim Roloff, Adrian Egli, Oliver Nolte, Christian R. Kahlert
Summary: In our tertiary children's hospital, the number of newly detected methicillin-resistant Staphylococcus aureus (MRSA) infections among primary and secondary school students has almost doubled in recent years. Through an epidemiological investigation and characterization analysis, a cluster of pneumococci with common genetic features was identified, with a specific school being identified as a common risk factor for infection. No further cases were found after disinfection.
SCIENTIFIC REPORTS
(2022)
Review
Microbiology
Opeyemi Uwangbaoje Lawal, Olaniyi Ayobami, Alaa Abouelfetouh, Nadira Mourabit, Mamadou Kaba, Beverly Egyir, Shima M. Abdulgader, Adebayo Osagie Shittu
Summary: This study provides an update on the clonal distribution of MRSA in Africa, identifying diverse genotypes of MRSA across the continent with ST5 and ST8 clones being commonly found. There is a need to strengthen genomic surveillance capacity to prevent and control the spread of MRSA.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ching-Hsi Hsiao, Eugene Yu-Chuan Kang, Lung-Kun Yeh, David H. K. Ma, Hung-Chi Chen, Kuo-Hsuan Hung, Yhu-Chering Huang
Summary: This study investigated the genotyping, antibiotic susceptibility, and clinical features of Staphylococcus aureus keratitis, with a focus on the role of Panton-Valentine leucocidin (PVL). The results showed that approximately one third of S. aureus keratitis in Taiwan was caused by community-associated MRSA. Although MRSA isolates were more resistant than MSSA, the clinical characteristics were similar between the two groups. Fluoroquinolones could be a good empirical antibiotic choice for S. aureus keratitis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Emma McGuire, Claire Neill, Simon M. Collin, Hannah Higgins, Rebecca Guy, Mark Ganner, Juliana Coelho, Bruno Pichon, Russell Hope, Colin S. Brown
Summary: PVL toxin detection was not associated with worse outcomes in patients with community-acquired S. aureus bacteremia.
JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Article
Microbiology
Utta Helbig, Constance Riemschneider, Guido Werner, Nancy Kriebel, Franziska Layer-Nicolaou
Summary: In Saxony, Germany, the detection of PVL-positive MRSA must be reported to the local health authority, which then takes appropriate measures to control the infection. Isolates from 2019 cases were analyzed, and various MRSA lineages with PVL were identified. Most cases were skin and soft-tissue infections and had a history of travel or migration. The presence of the same MRSA clone in households suggested a close epidemic link. Reporting PVL-positive MRSA allows for early detection and targeted control measures.
Article
Infectious Diseases
Rajiha A. Ibrahim, Nega Berhe, Zelalem Mekuria, Eyasu Seyoum, Joan-Miquel Balada-Llasat, Tamrat Abebe, Solomon H. Mariam, Estifanos Tsige, Surafel Fentaw Dinku, Shu-Hua Wang
Summary: This study investigates the antimicrobial resistance and virulence genes of isolated strains in Ethiopia, finding high resistance to oral antibiotics and the prevalence of pvl, seb, sea, and TSST-1 genes.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Public, Environmental & Occupational Health
J. Hatcher, S. Godambe, H. Lyall, L. Tyszczuk, F. Stubbs, N. Cummings, K. Sheils, C. Hughes, T. Kadhani, S. Atkin, N. Elaby, B. Pichon, B. Patel, E. T. Brannigan
Summary: The novel coronavirus (SARS-CoV-2) is a newly emerged coronavirus that was first discovered in Wuhan, China and has caused a global pandemic. It causes COVID-19, an infectious disease that can result in symptoms such as fever, cough, and difficulty breathing. The COVID-19 pandemic has had a significant impact on global healthcare systems and economies, leading many countries to implement strict isolation and control measures, including travel restrictions, lockdowns, and social distancing measures.
JOURNAL OF HOSPITAL INFECTION
(2022)
Article
Agriculture, Dairy & Animal Science
Abraham Fikru Mechesso, Su-Jeong Kim, Ho-Sung Park, Ji-Hyun Choi, Hyun-Ju Song, Mi Hyun Kim, Suk-kyung Lim, Soon-Seek Yoon, Dong-Chan Moon
Summary: This study identified 199 Staphylococcus aureus isolates from quarter milk samples of 1,289 dairy cattle between 2014 and 2018, and found 30 MRSA strains. The research suggests that dairy cows could be a major source for spreading MRSA, indicating the need for enhanced hygienic measures.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Martina Maritati, Marco Manfrini, Maria Rosa Iaquinta, Alessandro Trentini, Silva Seraceni, Matteo Guarino, Anna Costanzini, Roberto De Giorgio, Gustavo Alberto Zanoli, Alessandro Borghi, Elisa Mazzoni, Giuseppe De Rito, Carlo Contini
Summary: The aim of this study was to investigate the effect of Staphylococcus aureus (SA) producing the Panton-Valentine leukocidin (PVL) on the outcome of Prosthetic Joint Infection (PJI). The study found that SA strains positive for PVL genes resulted in a 100% failure rate in the treatment of acute PJI, while chronic PJI had a 100% eradication rate. Therefore, toxin research and targeted antibiotic therapy should be considered in the treatment of PJI caused by SA.
Review
Infectious Diseases
Pierre Louis Conan, Isabelle Podglajen, Fabrice Compain, Mehdi Osman, David Lebeaux, Edouard Flamarion
Summary: This is the first reported case of a renal abscess caused by Panton-Valentine leukocidin-producing Staphylococcus aureus in an immunocompetent patient. Treatment with percutaneous drainage and an antibiotic with toxin inhibiting effect was successful, leading to rapid improvement in the patient and complete resolution of the abscess within 6 months.
INFECTIOUS DISEASES
(2021)
Article
Immunology
Nancy Evelyn Aguilar-Gomez, Jocelin Merida-Vieyra, Oscar Daniel Isunza-Alonso, Maria Gabriela Morales-Pirela, Oscar Colin-Martinez, Enrique Josue Juarez-Benitez, Silvestre Garcia de la Puente, Alejandra Aquino-Andrade
Summary: This study describes the phenotypic and genotypic characteristics of Staphylococcus aureus isolates from osteoarticular infections in paediatric patients. The majority of isolates were methicillin-sensitive S. aureus, and the pvl gene was only observed in these isolates. The study highlights the importance of a multidisciplinary team for the diagnosis and treatment of osteoarticular infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Pediatrics
Massimo Luca Castellazzi, Samantha Bosis, Irene Borzani, Claudia Tagliabue, Raffaella Pinzani, Paola Marchisio, Giada Maria di Pietro
Summary: This is a case report of a 6-month-old boy with severe invasive PVL-SA infection, presenting with high fever and poor general condition. The importance of prompt diagnosis and appropriate management in order to save lives and prevent serious complications is highlighted in this report. The patient received intravenous antibiotics and surgical drainage of abscess with progressive recovery.
ITALIAN JOURNAL OF PEDIATRICS
(2021)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)