Article
Microbiology
Shlomo Pilo, Gal Zizelski Valenci, Mor Rubinstein, Lea Pichadze, Yael Scharf, Zeev Dveyrin, Efrat Rorman, Israel Nissan
Summary: This study improved Bom's MLST scheme by using newly designed primers and stringent conditions, enhancing the detection performance for samples with low concentrations of Chlamydia DNA. A simple scoring scheme and flow chart were developed based on Real-Time PCR analysis values to identify reaction inhibitors and predict amplification success. The new MLST version showed great results in patients attending an STI clinic.
Article
Microbiology
Laura Martinez-Garcia, Jose Maria Gonzalez-Alba, Teresa Puerta, Alicia Comunion, Maria Concepcion Rodriguez-Jimenez, Eva Orviz, Matilde Sanchez-Conde, Mario Rodriguez-Dominguez, Rafael Canton, Juan Carlos Galan
Summary: This study proposed a new combination of molecular markers based on the most variable genes of L-genotype genomes to improve the characterization of the current LGV epidemic in Madrid, Spain. The new combination of molecular markers demonstrated higher diversity and improved discrimination of LGV strains compared to the previously published MLST scheme based on hypervariable genes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Luis Pineiro, Laura Villa, Paula Salmeron, Maria Dolores Macia, Luis Otero, Marti Vall-Mayans, Ana Milagro, Samuel Bernal, Ayla Manzanal, Inigo Ansa, Gustavo Cilla
Summary: This study assessed the dynamics of Chlamydia trachomatis infection transmission in Spain and its association with clinical and epidemiological variables. Genotype and sequence type distribution varied according to sex, sexual behavior, and geographical area. Sexual behavior was found to be an important factor in the transmission dynamics of C. trachomatis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Forestry
Liliana E. Garcia-Valencia, Jocelyn Perez-Garcia, Miguel Angel Vallejo-Reyna, Roberto Reynoso-Santos, Jesus Vargas-Hernandez, Florencia Garcia-Campusano
Summary: The study evaluated the utility of real-time PCR coupled with high-resolution melting (HRM) for identifying different varieties within the Pseudostrobus complex. The results showed that specific gene loci could successfully discriminate among different varieties, and also revealed the impact of geography on allele distribution and species hybridization.
Article
Fisheries
Ruixue Shi, Siqi Yang, Yanhe Li
Summary: In this study, the linkage disequilibrium and haplotype of SNP sites in the intron of the prophenoloxidase gene were analyzed in Procambarus clarkii. The detection of SNP_7081 genotype was found to be the most convenient and efficient way for WSSV-resistant group selection. The high-resolution melting curve (HRM) method was used for genotyping SNP_7081 site, but the appearance of false negatives hindered automatic grouping. By modifying the HRM method and increasing the GC content of the target fragment, an improved HRM method for genotyping SNP_7081 site that could automatically group was established. These findings provide new insights for HRM-based genotyping of SNPs.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Review
Medicine, General & Internal
Ying Zuo, Ting-Ting Jiang, Yang Teng, Yan Han, Yue-Ping Yin, Xiang-Sheng Chen
Summary: This study examined the association between CT serology and fertility-related and pregnancy adverse outcomes. It found that CT-specific IgG was significantly associated with tubal factor infertility and ectopic pregnancy.
Review
Immunology
Robert C. Brunham
Summary: This article discusses the approaches and challenges in developing a Chlamydia trachomatis vaccine, and summarizes two key research directions and unresolved issues in human C. trachomatis immunology.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Michelle Odonkor, Fahd Naufal, Beatriz Munoz, Harran Mkocha, Mabula Kasubi, Meraf Wolle, Sheila West
Summary: The survey in Kongwa District found a TF prevalence of 7.1%, with 21.5% of TF-positive children testing positive for infection. Seropositivity was 18.2% on average, with children born after MDA cessation showing a seroprevalence of 6.7%, indicating exposure to C. trachomatis despite previous interventions.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Biotechnology & Applied Microbiology
Mingyang Li, Wei Shi, Jia Yang, Qi Wang, Haiyan Dong, Jun Chen, Lifang Zhang, Shanli Zhu
Summary: The MOMP-targeted affibody molecule (Z(MOMP):461) screened in this study showed the ability to recognize native MOMP in cells infected with C. trachomatis, indicating great potential for delivering drugs for target therapy.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Tahereh Azimpour-Ardakan, Reza Fotouhi-Ardakani, Nasser Hoghooghi-Rad, Nourdehr Rokni, Abbasali Motallebi
Summary: HRM analysis using the B1 and ROP8 genes can accurately differentiate T. gondii genotypes. The nested-qPCR-HRM method showed the highest sensitivity and specificity compared to other methods, making it a powerful diagnostic tool for rapid detection and genotyping of T. gondii.
JOURNAL OF MICROBIOLOGICAL METHODS
(2021)
Article
Biochemistry & Molecular Biology
Stephan P. Verweij, Remco P. H. Peters, Arnold Catsburg, Henry J. C. de Vries, Sander Ouburg, Servaas A. Morre
Summary: This study aims to assess the potential association of MBL2 gene SNPs to Chlamydia trachomatis infection. The results showed clear associations between specific MBL2 genotypes, haplotypes, and different subgroups of C. trachomatis infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Maslin Osathanunkul, Nipitpong Sawongta, Nuankanya Sathirapongsasuti, Thunyaporn Poonsawat, Usawadee Detsri, Charatsee Aungtonya, Chatmongkon Suwannapoom
Summary: Accurate species identification is crucial for studying the distribution of highly venomous jellyfish. This study developed a molecular method called high resolution melting (HRM) to differentiate various jellyfish species, providing a more practical and effective approach compared to traditional methods.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Materials Science, Multidisciplinary
E. C. Chukukere, A. Omame, C. P. Onyenegecha, S. C. Inyama
Summary: A model for the co-dynamics of Chlamydia trachomatis (CT) and Gonorrhea was studied, with optimal control analysis used to assess the impact of targeted treatment on co-infections. Different intervention strategies were analyzed, with some leading to a reduction in cases of co-infection.
RESULTS IN PHYSICS
(2021)
Article
Public, Environmental & Occupational Health
George M. Fistonich, Kenya M. Troutman, Adam J. Visconti
Summary: During the COVID-19 pandemic, mail-out HIV and sexually transmitted infection testing was well-received in the District of Columbia among high-priority demographics. Results from the initial phase showed a significant uptake of testing, with a proportion of individuals testing positive for sexually transmitted infections.
AMERICAN JOURNAL OF PREVENTIVE MEDICINE
(2021)
Article
Infectious Diseases
Kathleen Lynch, Sue Chen M. Apadinuwe, Stephen Lambert, Tessa Hillgrove, Mitchell Starr, Beth Catlett, Robert Ware, Anasaini W. Cama, Sara Webster, Emma M. Harding-Esch, Ana Bakhtiari, Robert Butcher, Philip Cunningham, Diana Martin, Sarah Gwyn, Anthony Solomon, Chandalene Garabwan, John Kaldor, Susana Vaz Nery
Summary: The Nauruan children are heavily affected by active trachoma caused by ocular infection with C. trachomatis. The epidemiology of trachoma in Nauru differs from other endemic settings, with a high prevalence of clinical signs but limited evidence of chlamydial infection. The study suggests that comprehensive public health intervention is required to control trachoma in Nauru.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)