Dipicolinic acid-based disk methods for detection of metallo-beta-lactamase-producing Pseudomonas spp. and Acinetobacter spp.

We evaluated novel and simple dipicolinic acid (DPA)-based phenotypic screening methods to detect metallo-beta-lactamase (MBL)-producing isolates: DPA disk synergy tests and DPA-based disk tests (DPA-imipenem, DPA-meropenem, or DPA-ceftazidime disk tests). Upon testing these methods on 79 MBL-producing and 95 MBL-nonproducing isolates of Pseudomonas spp. and Acinetobacter spp., their specificity and sensitivity were superior or comparable with those of the EDTA-based phenotypic screening methods. (C) 2008 Elsevier Inc. All rights reserved.