Journal
DIABETOLOGIA
Volume 57, Issue 11, Pages 2374-2383Publisher
SPRINGER
DOI: 10.1007/s00125-014-3356-z
Keywords
DNA methylation; Epigenetics; Glucose homeostasis; Human adipose tissue; LUMA; Subcutaneous; Visceral
Categories
Funding
- German Diabetes Association
- DDS Foundation
- IFB Adiposity Diseases [ADI-K50D, K7-45]
- EFSD (European Foundation for the Study of Diabetes)
- Federal Ministry of Education and Research (BMBF), Germany [FKZ: 01EO1001]
- Boehringer Ingelheim Foundation
- Kompetenznetz Adipositas (Competence Network for Obesity) - Federal Ministry of Education and Research (German Obesity Biomaterial Bank) [FKZ 01GI1128]
- Deutsche Forschungsgemeinschaft [SFB 1052/1, BO 3147/4-1]
- LIFE-Leipzig Research Center for Civilization Diseases, University of Leipzig
- European Union
- European Regional Development Fund (ERDF)
- Free State of Saxony within the framework of the excellence initiative
- Deutsche Hochdruckliga e.V.
- [ADI-K7-39]
- [K7-3]
- [K7-9]
- [K7-31]
- [ADI-K60E]
Ask authors/readers for more resources
Aims/hypothesis Epigenetic alterations may influence the metabolic pathways involved in human obesity. We hypothesised that global DNA methylation levels in adipose tissue might be associated with obesity and related phenotypes. Methods We measured global DNA methylation levels in paired samples of subcutaneous adipose tissue (SAT) and omental visceral adipose tissue (OVAT) from 51 individuals, and in leucocytes from 559 Sorbs, a population from Germany, using LUminometric Methylation Assay (LUMA). To further investigate the underlying mechanisms of the observed associations, we measured global methylation levels in 3T3-L1 adipocytes exposed to glucose, insulin and lipids. Results Global methylation levels (+/- SD) were significantly higher in OVAT (74.27%+/- 2.2%) compared with SAT (71.97%+/- 2.4%; paired t test, p<1 x 10(-9)). Furthermore, global methylation levels in SAT were positive correlates of measures of fat distribution (waist measurement, WHR) and glucose homeostasis (HbA(1c)) (all p<0.015 after accounting for multiple testing and covariates). Global methylation levels in the German Sorb cohort were associated with glucose homeostasis, but this association did not withstand adjustment for covariates. Exposure of 3T3-L1 adipocytes to insulin, palmitate and glucose decreased global methylation levels 1 h after treatment relative to controls. Conclusions/interpretation Our data suggest that the variability in global methylation in adipose tissue might be related to alterations in glucose metabolism.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available