Journal
DIABETOLOGIA
Volume 54, Issue 4, Pages 979-988Publisher
SPRINGER
DOI: 10.1007/s00125-010-2021-4
Keywords
Albuminuria; Diabetic nephropathy; Endothelin-1; Glomerular permeability; MCP-1; Nephrin; P-alb; sICAM-1
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Funding
- National Heart Lung and Blood Institute [HL69999, HL64776]
- Indiana Center for Biological Microscopy
- NIH
- American Heart Association
- government of Egypt
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Activation of endothelin receptor-A (ETA) increases glomerular permeability to albumin (P-alb) and elevates pro-inflammatory markers in hyperglycaemic rats. Male Sprague-Dawley rats were given streptozotocin (n = 32) or saline (sham; n = 32). Half of the animals in each group received the ETA-selective antagonist, ABT-627 (atrasentan; orally), beginning immediately after hyperglycaemia was confirmed. Glomeruli were isolated by sieving techniques and P-alb determined from the change in glomerular volume induced by oncotic gradients of albumin. Glomerular nephrin levels were assessed by immunofluorescence, whereas urinary nephrin was measured by immunoassay. At 3 and 6 weeks after streptozotocin injection, proteinuria was significantly increased compared with sham controls and significantly reduced by ABT-627 treatment. P-alb was also increased at 3 and 6 weeks post-streptozotocin. ABT-627 had no effect on P-alb or protein excretion in sham control rats. In glomeruli isolated from hyperglycaemic rats, incubation with BQ-123, a selective ETA antagonist, reduced P-alb, whereas BQ-788, a selective endothelin receptor-B antagonist had no effect (n = 6 rats per group, 5-8 glomeruli per rat). Glomerular and plasma content of soluble intercellular adhesion molecule-1 and monocyte chemoattractant protein-1 were significantly increased 6 weeks after streptozotocin (ELISA). ABT-627 attenuated these increases. After 6 weeks of hyperglycaemia, glomerular nephrin content was decreased with a concurrent increase in urinary nephrin excretion. ABT-627 prevented glomerular nephrin loss in hyperglycaemic rats (n = 5-8 rats per group; eight groups). These observations support the hypothesis that endothelin-1, via the ETA receptor, directly increases P-alb, possibly via nephrin loss, as well as early inflammation in the hyperglycaemic rat.
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