Article
Toxicology
Kei Takahashi, Yasuhiro Morita, Shuji Udagawa, Eriko Higashi, Mayumi Nakajima, Yohei Miyamoto, Keiyu Oshida
Summary: A research conducted overseas found that a drug called DMIP induces cell death and vacuolation in human aortic vascular smooth muscle cells. The vacuolation is caused by the accumulation of DMIP in the cells, and can be significantly reduced by using a vacuolar H+- ATPase inhibitor. The study also suggests that these vacuoles are mainly enlarged late endosomes/lysosomes, and not formed through autophagy.
TOXICOLOGY IN VITRO
(2023)
Article
Biochemistry & Molecular Biology
Thibault Courtellemont, Maria Giovanna De Leo, Navin Gopaldass, Andreas Mayer
Summary: Endo-lysosomal compartments exchange proteins through different processes, including fusion, fission, and endosomal transport carriers. The membrane fission events that occur during these processes are not well understood. This study identifies the CROP complex as a factor that plays a role in membrane fission. The CROP complex consists of members from two protein families and enhances the membrane fission activity of a specific protein. Disrupting the CROP complex prevents fragmentation of lysosome-like structures in yeast and impairs cargo export in mammalian endosomes.
Article
Cell Biology
Eva Alegre-Cortes, Alberto Gimenez-Bejarano, Elisabet Uribe-Carretero, Marta Paredes-Barquero, Andre R. A. Marques, Mafalda Lopes-da-Silva, Otilia V. Vieira, Saray Canales-Cortes, Pedro J. Camello, Guadalupe Martinez-Chacon, Ana Aiastui, Roberto Fernandez-Torron, Adolfo Lopez de Munain, Patricia Gomez-Suaga, Mireia Niso-Santano, Rosa A. Gonzalez-Polo, Jose M. Fuentes, Sokhna M. S. Yakhine-Diop
Summary: Myotonic dystrophy type 1 (DM1) is an autosomal dominant disease caused by CTG repeat expansion in the 3' untranslated region of the dystrophia myotonica protein kinase gene. Fibroblasts derived from DM1 patients show increased autophagy flux, enlarged endosomes, and lysosomes. The binding of EGF to EGFR and the internalization of EGFR are delayed in DM1 cells, but EGF-activated EGFR enhances the phosphorylation of AKT and ERK1/2.
Article
Engineering, Biomedical
Mi Hu, Fan Jia, Wei-Pin Huang, Xu Li, Deng-Feng Hu, Jing Wang, Ke-Feng Ren, Guo-Sheng Fu, Yun-Bing Wang, Jian Ji
Summary: The stiffness of the vascular extracellular matrix plays a crucial role in artery pathology and can impact the autophagy levels of vascular endothelial cells and smooth muscle cells. Increasing substrate stiffness leads to differential changes in autophagy levels, affecting gene expression associated with endothelial function and transitioning VSMCs from contractile to synthetic phenotype. Inhibiting cell autophagy further reduces endothelial functional gene expression and increases VSMC calponin expression, highlighting the important role of autophagy in response to changes in microenvironment stiffness.
BIOACTIVE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Navin Gopaldass, Maria Giovanna De Leo, Thibault Courtellemont, Vincent Mercier, Christin Bissig, Aurelien Roux, Andreas Mayer
Summary: Proteins exit from endosomes through retromer-coated tubular carriers, which affects cellular signaling, lysosomal biogenesis, and various diseases. The coat formation requires overcoming membrane tension. In this study, we investigated the dynamics and driving force of this process using yeast retromer, BAR-domain sorting nexins Vps5 and Vps17, and synthetic lipid tubules. The results showed that the coat forms a static tubular structure by bidirectional oligomerization, and high concentrations of sorting nexins constrict the membrane tubes. However, retromer oligomers are needed to drive constriction at lower concentrations.
Review
Biochemistry & Molecular Biology
Amitha Muraleedharan, Benoit Vanderperre
Summary: Parkinson's disease is the second most common neurodegenerative disorder after Alzheimer's disease, and its prevalence increases with age. The endolysosomal system plays a major role in the pathogenesis of PD, and studying the genes and proteins involved in this system can lead to potential therapeutic interventions.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Irina Zakharova, Shoraan Saaya, Alexander Shevchenko, Alena Stupnikova, Maria Zhiven', Pavel Laktionov, Alena Stepanova, Alexander Romashchenko, Lyudmila Yanshole, Alexander Chernonosov, Alexander Volkov, Elena Kizilova, Evgenii Zavjalov, Alexander Chernyavsky, Alexander Romanov, Andrey Karpenko, Suren Zakian
Summary: This study demonstrates the potential use of discarded cardiac tissue for regenerative medicine and tissue engineering. The researchers investigated the benefits of using cardiac explants to repair vascular defects and tested a method to prevent cell division using mitomycin C. The results showed that the cell-containing patches engrafted successfully and did not interfere with normal patency. Mitomycin C-treated cells exhibited no tumorigenic effect and retained their specific cell markers, extracellular matrix production, and ability to stimulate angiogenesis. This approach may contribute to the development of safe cell products for regenerative medicine.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Tong Li, Baofu Wang, Hao Ding, Shiqi Chen, Weiting Cheng, Yang Li, Xiaoxiao Wu, Lei Wang, Yangyang Jiang, Ziwen Lu, Yu Teng, Sha Su, Xiaowan Han, Mingjing Zhao
Summary: Atherosclerosis (AS)-related diseases are the leading cause of death in clinical patients. Vascular smooth muscle cells (VSMCs) play a crucial role in AS, and intercellular communication through extracellular vesicles (EVs) is important in the pathophysiology of AS.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Cell Biology
Yu-Xia Zhang, Ri-Ning Tang, Li-Ting Wang, Bi-Cheng Liu
Summary: Chronic kidney disease (CKD) is a significant health issue globally, with vascular calcification (VC) playing a major role in the cardiovascular morbidity and high mortality of CKD. Intercellular crosstalk between ECs and SMCs is crucial in the development of VC associated with CKD.
CELL PROLIFERATION
(2021)
Article
Microbiology
Rui-cong Sun, Jia-huan Hu, Xiao-han Li, Chun-chun Liu, Ya-yun Liu, Jing Chen, Yi-chen Yang, Bin Zhou
Summary: This study demonstrates for the first time that VCP is essential for the infection of CSFV and plays a key role in its intracellular transport. The findings provide an alternative approach for the discovery of novel anti-flaviviridae drugs.
VETERINARY MICROBIOLOGY
(2022)
Article
Biophysics
Xiaoyu Wang, Bin Gao, Xiang-kui Ren, Jintang Guo, Shihai Xia, Wencheng Zhang, Cheng Yang, Yakai Feng
Summary: This study developed a strategy for synchronizing promotion of endothelial cells and inhibition of smooth muscle cells, providing a new approach for the treatment of vascular restenosis.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Ningning Sun, Beyongsam Chu, Dong-Hyun Choi, Leejin Lim, Heesang Song
Summary: Abnormal communication between endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) promotes vascular diseases. ETS variant transcription factor 2 (ETV2) plays a role in pathological angiogenesis, but its role in the communication between ECs and VSMCs is unknown. CXCL5 induced by ETV2 in ECs promotes VSMC migration through MMP upregulation and the activation of Akt and p38/c-Jun.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biotechnology & Applied Microbiology
Laura Locatelli, Sara Castiglioni, Jeanette A. M. Maier
Summary: This review article discusses the effects of space environment on the human vasculature and the adaptive responses activated in such conditions. The study suggests that oxidative stress and mitochondrial dysfunction might be mediators of vascular alterations in space. Understanding the cellular and molecular mechanisms involved in these adaptive processes could provide insights for designing proper countermeasures for space exploration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Priya Raman, Saugat Khanal
Summary: Leptin plays a crucial role in the pathogenesis of atherosclerotic complications associated with obesity and diabetes. Elevated levels of leptin can lead to vascular dysfunction and the development of atherosclerosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Roger M. Pallares, David Faulkner, Dahlia D. An, Solene Hebert, Alex Loguinov, Michael Proctor, Jonathan A. Villalobos, Kathleen A. Bjornstad, Chris J. Rosen, Christopher Vulpe, Rebecca J. Abergel
Summary: Lanthanides, a group of critical elements widely used in various industries, have been found to potentially impact human health. This study used a functional toxicogenomics approach in baker's yeast to assess the toxicity mechanisms of lanthanides, revealing different trends and effects on cellular responses. The research highlighted the disruption of vesicle-mediated transport and biosynthetic pathways as key functions affected by lanthanides, with implications for potential targeting of human orthologs by these elements.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)