Article
Multidisciplinary Sciences
Bor-Uei Shyr, Bor-Shiuan Shyr, Shih-Chin Chen, Yi-Ming Shyr, Shin-E Wang
Summary: The study found that pancreas transplant alone in uremic (PTAU) patients had lower 5-year patient survival rates, while the pancreas graft survival rates were higher but needed to consider if deaths were censored. It is recommended not to recommend PTAU unless other options are not available.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, General & Internal
Jennifer McVean, Gregory P. Forlenza, Roy W. Beck, Colleen Bauza, Ryan Bailey, Bruce Buckingham, Linda A. DiMeglio, Jennifer L. Sherr, Mark Clements, Anna Neyman, Carmella Evans-Molina, Emily K. Sims, Laurel H. Messer, Laya Ekhlaspour, Ryan McDonough, Michelle Van Name, Diana Rojas, Shannon Beasley, Stephanie DuBose, Craig Kollman, Antoinette Moran, CLVer Study Grp
Summary: The study aimed to determine the effectiveness of intensive diabetes management to achieve near normalization of glucose levels on preservation of pancreatic beta cell function in youth with newly diagnosed type 1 diabetes. The results showed that although the intensive management group achieved excellent glucose control, it did not affect the decline in pancreatic C-peptide secretion at 52 weeks.
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
(2023)
Article
Endocrinology & Metabolism
Jie Yu, Yiwen Liu, Lu Lyu, Yuan Zhao, Mengya Qi, Fan Ping, Lingling Xu, Wei Li, Qiang Xu, Huabing Zhang, Wenming Wu, Yuxiu Li
Summary: New and optimized diagnostic criteria for endogenous hyperinsulinemic hypoglycemia in the Chinese population have been proposed, and these criteria yield satisfactory accuracy.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Medical Laboratory Technology
Delhia X. Nkuna, Siyabonga P. Khoza, Jaya A. George, Mpho R. Maphayi
Summary: This study aimed to investigate the impact of sample type, storage temperature, and time delays on the stability of C-peptide and insulin. The results showed that C-peptide was more stable in separated serum than plasma, with a PD of -5% and -13% at 2-8 degrees C for 7 days, while it was most unstable at room temperature after 48 h. Insulin was found to be more stable in plasma than in serum, with a minimum PD of -1% when stored at -20 degrees C for 30 days. When samples were kept unspun at room temperature for 72 h, PD was -23% and -80% in plasma and serum, respectively.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Endocrinology & Metabolism
Katsiaryna Prystupa, Rebecka Renklint, Youssef Chninou, Julia Otten, Louise Fritsche, Sebastian Hoerber, Andreas Peter, Andreas L. Birkenfeld, Andreas Fritsche, Martin Heni, Robert Wagner
Summary: This study aims to validate various surrogate indices based on OGTT and fasting measurements by comparing them with gold standard methods. The results showed that certain indices have strong correlation with the gold standard measures and could be potentially used in future research and clinical practice.
BMJ OPEN DIABETES RESEARCH & CARE
(2022)
Review
Endocrinology & Metabolism
Ernesto Maddaloni, Geremia B. Bolli, Brian M. Frier, Randie R. Little, Richard D. Leslie, Paolo Pozzilli, Raffaela Buzzetti
Summary: C-peptide measurement is valuable in autoimmune diabetes but limited in type 2 diabetes due to confounding factors like insulin resistance. Standardization issues exist that may affect comparability between different laboratories.
DIABETES OBESITY & METABOLISM
(2022)
Article
Automation & Control Systems
Ali R. Jalalvand, Licarion Pinto
Summary: A sensitive and selective electrochemical biosensor was developed for the simultaneous detection of insulin (IN), proinsulin (PIN), and C-peptide (CPT) using a modified thick-film boron doped diamond electrode (TFBDDE). The TFBDDE was modified with multiwalled carbon nanotubes-fullerene C-60-graphene-ionic liquid (MWCNTs-C-60-GR-IL) and triple template molecules were synthesized on the sensor surface using UV polymerization. The biosensor showed excellent performance in simultaneous determination of IN, PIN, and CPT in both artificial and real human serum samples using multi-way calibration methods.
CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS
(2023)
Article
Endocrinology & Metabolism
Yihan Wei, Li Quan, Ting Zhou, Guoli Du, Sheng Jiang
Summary: This study investigated the insulin requirement profiles of T2DM patients with different C-peptide levels on insulin pump therapy. It found differences in basal premeal dose ratio between groups, as well as correlations between C-2h/C-0 and waist circumference, HbA1c, FPG, and 2hPG. BMI and FPG were identified as independent factors influencing %TBa and C-2h/C-0.
NUTRITION & DIABETES
(2021)
Article
Biochemistry & Molecular Biology
Mohammed F. Gholam, Niharika Bala, Yunus E. Dogan, Abdel A. Alli
Summary: The expression of the MARCKS family of proteins in the kidneys is important for regulating the renal epithelial sodium channel (ENaC) and blood pressure. MARCKS function is controlled by post-translational modifications such as myristoylation, phosphorylation, and proteolysis. Proteases that cleave ENaC and MARCKS have been implicated in hypertension. This study investigated MARCKS protein expression and proteolysis, PKC isoform expression, and protease expression and activity in diabetic db/db mouse kidneys. The results suggest that increased proteolysis of renal MARCKS, upregulation of the cathepsin proteolytic pathway, and increased PKC expression may contribute to the development of high blood pressure in diabetes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Nesrin Damla Karakaplan, Yilin Song, Marcello C. Laurenti, Adrian Vella, Michael D. Jensen
Summary: This study aimed to evaluate the effects of exogenous hyperinsulinemia on insulin secretion and determine whether the response differs in insulin resistance associated with obesity. The results showed that hyperinsulinemia suppresses endogenous insulin secretion, and the relative change in insulin secretion produced by exogenous insulin does not differ between nonobese and obese individuals.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Endocrinology & Metabolism
Fideline Bonnet-Serrano, Clara Devin-Genteuil, Louis Thomeret, Christelle Laguillier-Morizot, Marie-Clemence Leguy, Anna Vaczlavik, Lucas Bouys, Corinne Zientek, Leopoldine Bricaire, Laura Bessiene, Laurence Guignat, Rossela Libe, Helen Mosnier-Pudar, Guillaume Assie, Lionel Groussin, Jean Guibourdenche, Jerome Bertherat
Summary: Diagnosis of endogenous hyperinsulinism relies on a hypoglycemia with low insulin and C-peptide levels. Optimized cutoffs were proposed for glycemia, insulin, and C-peptide levels for diagnosis. C-peptide level of 0.3 nmol/L with a hypoglycemia <2.3 mmol/L appears to be the best criterion for diagnosing endogenous hyperinsulinism.
EUROPEAN JOURNAL OF ENDOCRINOLOGY
(2023)
Article
Endocrinology & Metabolism
Berthold Hocher, Yong-Ping Lu, Christoph Reichetzeder, Xiaoli Zhang, Oleg Tsuprykov, Jan Rahnenfuhrer, Li Xie, Jian Li, Liang Hu, Bernhard K. Kraemer, Ahmed A. Hasan
Summary: The study suggests that paternal genetic defects, such as eNOS deficiency, may alter the epigenome of sperm without transmitting the paternal genetic defect itself. In later life, wild-type male offspring of +/- eNOS fathers developed increased fasting insulin and increased insulin after glucose load.
Review
Biochemistry & Molecular Biology
Marcello C. Laurenti, Aleksey Matveyenko, Adrian Vella
Summary: Pancreatic beta-cells are responsible for insulin synthesis and exocytosis in response to glucose, with pulsatile insulin secretion being a marker of beta-cell health. The challenges of measuring fast oscillatory secretion in vivo include anatomical difficulties and the need for mathematical deconvolution. Targeting impaired pulsatile secretion has therapeutic implications and can serve as an early diagnostic indicator of beta-cell stress.
Article
Engineering, Biomedical
Daniel Schneditz, Longin Niemczyk, Stanislaw Niemczyk
Summary: Modeling analysis revealed significant differences in insulin secretion and glucose utilization in patients with type 2 diabetes during hemodialysis, but overall there were only small differences in glucose and insulin balance.
BIOCYBERNETICS AND BIOMEDICAL ENGINEERING
(2021)
Review
Endocrinology & Metabolism
Linda Wu, Venessa Tsang, Alexander M. Menzies, Sarah C. Sasson, Matteo S. Carlino, David A. Brown, Roderick Clifton-Bligh, Jenny E. Gunton
Summary: Checkpoint inhibitor-associated autoimmune diabetes mellitus (CIADM) is a rare complication of immune checkpoint inhibitor therapy. This systematic review identified that CIADM commonly presents in diabetic ketoacidosis, and positivity of T1D autoantibodies is associated with earlier diagnosis.
Article
Biochemistry & Molecular Biology
Takeshi Yoneshiro, Woongchul Shin, Ken Machida, Keigo Fukano, Ayumi Tsubota, Yong Chen, Hironobu Yasui, Osamu Inanami, Yuko Okamatsu-Ogura, Kazuhiro Kimura
Article
Biochemistry & Molecular Biology
Tomoki Bo, Tohru Yamamori, Kumiko Yamamoto, Masaki Fujimoto, Hironobu Yasui, Osamu Inanami
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Kota Kimura, Nami Iguchi, Hitomi Nakano, Hironobu Yasui, Shingo Matsumoto, Osamu Inanami, Hiroshi Hirata
Summary: An accelerated imaging system using EPR and nitroxyl radicals was established for redox-sensitive mapping in a mouse tumor model. Sparse sampling technique was successfully applied to evaluate solution phantom and mouse xenograft models, achieving fourfold acceleration in 3D redox-sensitive mapping compared to conventional methods.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Biology
Masaki Nagane, Hironobu Yasui, Periannan Kuppusamy, Tadashi Yamashita, Osamu Inanami
Summary: Studies have shown that low-dose radiation exposure increases the risk of cardiovascular diseases, especially those related to the heart and blood vessels. The cellular senescence of vascular endothelial cells and activation of endothelial nitric oxide synthase following radiation exposure may play key roles in the pathogenesis of cardiovascular diseases.
JOURNAL OF RADIATION RESEARCH
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Tomoki Bo, Hironobu Yasui, Tohru Shiga, Yuki Shibata, Masaki Fujimoto, Motofumi Suzuki, Kei Higashikawa, Naoki Miyamoto, Osamu Inanami, Yuji Kuge
Summary: Eribulin significantly reduced tumor hypoxia by improving tumor oxygenation, enhancing the antitumor activity of radiation.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)
Article
Oncology
Tatsuya Deguchi, Kenji Hosoya, Shango Kim, Yusuke Murase, Kumiko Yamamoto, Tomoki Bo, Hironobu Yasui, Osamu Inanami, Mahiro Okumura
Summary: Metformin has significant radio-sensitizing effects on CSCs by inhibiting mitochondrial function, resulting in decreased oxygen consumption, decreased mitochondrial membrane potential, and decreased ATP production in SCs. Additionally, SCs have a higher mitochondrial respiration capacity than ACs, leading to differences in sensitivity to metformin and radiation therapy.
MOLECULAR THERAPY-ONCOLYTICS
(2021)
Article
Oncology
Tomoki Bo, Sho Kobayashi, Osamu Inanami, Junichi Fujii, Osamu Nakajima, Tsunekata Ito, Hironobu Yasui
Summary: LAT1 is crucial for transporting neutral amino acids into cells and is associated with cancer malignancy. JPH203, a novel drug targeting LAT1, has shown to inhibit tumor growth and increase cancer cell sensitivity to radiation. The combination of JPH203 and radiation therapy may be a potent treatment strategy for cancer.
TRANSLATIONAL ONCOLOGY
(2021)
Article
Chemistry, Physical
Osamu Inanami, Wakako Hiraoka, Yuto Goto, Hideo Takakura, Mikako Ogawa
Summary: Photoimmunotherapy (PIT) is a novel cancer therapy that utilizes tumor-specific monoclonal antibodies and a silicon phthalocyanine derivative to induce cell death through NIR-induced formation of IR700 anion radicals. Experimental results confirm the importance of these anion radicals in inducing the aggregation of IR700 and generating O-2(.-) under certain conditions.
Article
Neurosciences
Mayuko Hashimoto, Masaki Fujimoto, Kohtarou Konno, Ming-Liang Lee, Yui Yamada, Koya Yamashita, Chitoku Toda, Michio Tomura, Masahiko Watanabe, Osamu Inanami, Hiroshi Kitamura
Summary: This study reveals that hypothalamic USP2 regulates blood glucose levels through the ROS-AMPK-sympathetic nerve axis. Inhibition of USP2 leads to mitochondrial dysfunction and AMPK phosphorylation, resulting in increased blood glucose and norepinephrine levels.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Oncology
Masaki Fujimoto, Ritsuko Higashiyama, Hironobu Yasui, Koya Yamashita, Osamu Inanami
Summary: The study showed that inhibiting glutaminolysis enhanced radiation-induced cell death in KRAS-mutated lung cancer cell lines. Combination treatment with a glutaminolysis inhibitor and a senolytic drug could be useful for efficient radiotherapy.
TRANSLATIONAL ONCOLOGY
(2022)
Article
Chemistry, Medicinal
Takuya Kato, Ryuhei Okada, Yuto Goto, Aki Furusawa, Fuyuki Inagaki, Hiroaki Wakiyama, Hideyuki Furumoto, Dagane Daar, Baris Turkbey, Peter L. Choyke, Hideo Takakura, Osamu Inanami, Mikako Ogawa, Hisataka Kobayashi
Summary: NIR-PIT utilizes molecularly targeted antibodies with a photosensitive dye to target cancer cells, causing irreversible damage and highly immunogenic cell death through near-infrared light exposure. The use of L-NaAA can suppress acute edema, accelerate ligand release, and enhance therapeutic effects without causing side effects from unnecessary ROS production.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE
(2021)
Article
Veterinary Sciences
Yayoi Otsuka-Yamasaki, Osamu Inanami, Haruka Shino, Reeko Sato, Masahiro Yamasaki
Summary: In this study, we investigated the effects of a CYB5R3 mutation in a Pomeranian dog family with hereditary methemoglobinemia. The mutant enzyme showed decreased heat stability and increased susceptibility to trypsin hydrolysis, suggesting a higher sensitivity to temperature-dependent denaturation compared to the wild-type form. These findings suggest that the unstable mutant enzyme properties with normal enzymatic activity may be associated with hereditary methemoglobinemia in the Pomeranian dog family.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2021)
Article
Nutrition & Dietetics
Masaki Fujimoto, Tomoki Bo, Kumiko Yamamoto, Hironobu Yasui, Tohru Yamamori, Osamu Inanami
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION
(2020)
Article
Veterinary Sciences
Akihiro Kamikawa, Jumpei Seko
EXPERIMENTAL ANIMALS
(2020)
Article
Physiology
Mayuko Hashimoto, Natsuko Saito, Haru Ohta, Kumiko Yamamoto, Asuka Tashiro, Kosuke Nakazawa, Osamu Inanami, Hiroshi Kitamura
PHYSIOLOGICAL REPORTS
(2019)