Journal
DEVELOPMENTAL DYNAMICS
Volume 239, Issue 6, Pages 1807-1817Publisher
WILEY
DOI: 10.1002/dvdy.22298
Keywords
myosin heavy chain gene; muscle development; hedgehog signaling pathway; muscle pioneer; horizontal myoseptum; engrailed; adaxial cell; medaka; Oryzias latipes
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Funding
- Japan Society for the Promotion of Science [19108001, 18780160, 19-04576]
- Grants-in-Aid for Scientific Research [18780160] Funding Source: KAKEN
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We cloned three full-length cDNAs encoding myosin heavy chains (MYHs) previously found to be expressed in embryos or larvae of medaka Oryzias latipes. Based on cDNA sequence information, the three medaka MYH genes, mMYH(emb1), mMYHL(L1) and mMYH(L2) were localized on the chromosomes. In vivo promoter assay using the gene encoding green or red fluorescent protein and linked to the 5'-flanking region of mMYH demonstrated that the transcripts of fast-type mMYH(emb1), first expressed in embryos but belonging to the adult type in phylogenetic analysis, were located in the horizontal myoseptum. On the other hand, embryonic fast-type mMYH(L1) and mMYH(L2) were expressed in the whole myotomes. Interestingly, cells expressing mMYH(emb1) were localized together with engrailed, and cyclopamine, which blocks hedgehog signaling, inhibited mMYH(emb1) expression as well as the formation of the horizontal myoseptum, suggesting that muscle pioneer cells express mMYH(emb1) as a key protein in the formation of the horizontal myoseptum. Developmental Dynamics 239:1807-1817, 2010. (C) 2010 Wiley-Liss, Inc.
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