Journal
DEVELOPMENTAL DYNAMICS
Volume 237, Issue 4, Pages 1183-1192Publisher
WILEY
DOI: 10.1002/dvdy.21514
Keywords
lmx1b; limb development; nail-patella syndrome; microarray; Ntnl; Calb2; Ebf2; Rspo1; Sfrp1; Pax9; vcan; Laphn3
Categories
Ask authors/readers for more resources
In the developing limb, dorsal-ventral patterning is controlled by the transcription factor LMX1B, expressed in the dorsal mesenchyme. Loss of Lmx1b function in mice or humans results in the loss of dorsal limb structures and Nail-Patella syndrome, but the effectors through which LMX1B controls limb patterning are virtually unknown. Using microarrays to analyze the differential expression of mRNAs in wild-type vs. Lmx1b (-/-) limb buds, we have identified hundreds of genes as putative LMXB targets. Analysis of a subset of these candidates by in situ mRNA localization has identified eight genes previously unknown to require Lmx1b for their dorsal-ventral restriction of expression in the limb. Furthermore, our results suggest that LMX1B controls different targets along the proximal-distal axis of the limb, and suggest the existence of a dorsal proximal limb region that is rich in mRNAs requiring Lmx1b for their expression.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available