Article
Developmental Biology
Zhi Ye, Christopher R. Braden, Andrea Wills, David Kimelman
Summary: During early embryogenesis, the vertebrate embryo extends from anterior to posterior due to the addition of cells from a posterior neuromesodermal progenitor population. The autoregulatory loop between Wnt and Brachyury/Tbxt is crucial for maintaining mesodermal potential and normal axis development. Hox13 genes support body axis formation and the autoregulatory loop, with over 500 potential Hox13 target genes identified in zebrafish.
Correction
Biology
Qiuyu Guo, Albert Kim, Bin Li, Andrew Ransick, Helena Bugacov, Xi Chen, Nils Lindstrom, Aaron Brown, Leif Oxburgh, Bing Ren, Andrew P. McMahon
Summary: The study reveals that beta-catenin plays a crucial role in regulating self-renewal and differentiation of mammalian nephron progenitor cells. The modulation of beta-catenin levels using the GSK3 inhibitor CHIR showed opposing effects on NPC development, with low CHIR promoting maintenance and expansion while high CHIR inducing differentiation. Additionally, the study found that in high CHIR conditions, beta-catenin interacts with TCF7/LEF1 complexes to activate enhancers of differentiation-promoting target genes.
Article
Biotechnology & Applied Microbiology
Keisuke Fukada, Koji Tachibana, Yuta Kurashina, Yosuke Kaneko, Tatsuaki Matsumoto, Takeshi Miyamoto, Yasuo Niki, Masaya Nakamura, Hiroaki Onoe
Summary: This paper presents a scalable microfiber-shaped tissue for macroscale tendon tissue reconstruction in vitro. The microfiber-shaped tendon-like tissues maintained their morphology and showed proper expression of tenomodulin and actin filaments. A macro-scale tendon tissue assembly was successfully constructed by bundling the microfiber-shaped tendon-like tissues, allowing for uniform cell distribution.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Heng Liu, Peter E. Mueller, Attila Aszodi, Roland M. Klar
Summary: This study aimed to investigate the biological mechanism of growth factors in osteochondral regeneration. The results showed the modulatory effect of BMP-2 and TGF-beta 3 on the osteochondral process, as well as the downregulation of BMP-2 activity by Noggin. Interestingly, there was a synergistic effect between TGF-beta 3 and Noggin, suggesting that signals change their functions throughout the tissue formation process.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Sumeyra Nur Fuerkaiti, Anil Sera Cakmak, Cagatay Karaaslan, Menemse Gumusderelioglu
Summary: This study demonstrated that silencing Noggin using siRNA induces osteogenic differentiation in reduced doses of BMP-2 for scaffold-based bone regeneration. The siNoggin-transfected cells showed enhanced osteogenic effects and mineralization, suggesting a potential safe therapeutic approach for tissue regeneration.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Cell Biology
Jinshu Xu, Jun Li, Aarthi Ramakrishnan, Hanen Yan, Li Shen, Pin-Xian Xu
Summary: The vertebrate Six1 and Six2 genes have different functions in kidney development. While both genes are expressed in nephron progenitors in human fetal kidneys, the expression patterns differ in mouse kidneys. Forced expression of Six1 in Six2-deficient kidneys was unable to rescue the kidney phenotype, indicating that Six1 cannot substitute Six2 in driving nephrogenesis in mice. These findings suggest that the difference in the physiological roles of Six1 and Six2 in kidney development is due to both transcriptional regulation and divergent biochemical properties of the proteins.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Materials Science, Biomaterials
Anil Sera Cakmak, Sumeyra Fuerkaiti, Dilara Karaguzel, Cagatay Karaaslan, Menemse Gumusderelioglu
Summary: This study demonstrates that the CRISPR/Cas9 mechanism can be used to knockout the Noggin gene, thereby increasing the activity of BMP-2 signaling and promoting osteogenic differentiation. The findings suggest that CRISPR/Cas9 has great potential as a tool for obtaining new cell sources for tissue engineering studies.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Min Gyu Lee, Hwamok Oh, Jong Woo Park, Jueng Soo You, Jeung-Whan Han
Summary: Nuclear S6K1 functions in the regulation of Wnt target gene expression and has an impact on cell proliferation and invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Qian Liu, Yaxi Zhu, Weihong Zhu, Ge Zhang, Yunzhi Peter Yang, Chunfeng Zhao
Summary: Tendon injuries are common musculoskeletal disorders that significantly impact patients' quality of life. MicroRNAs play a vital role in biological processes and may serve as an innovative strategy for tendon pathologies. Advancements in miRNA-based therapeutics could lead to new approaches for the treatment of tendon disorders.
Article
Chemistry, Multidisciplinary
Zhirui Dong, Ruoxuan Peng, Yuehua Zhang, Yicheng Shan, Wang Ding, Yifan Liu, Jian Li, Mingdong Zhao, Li-Bo Jiang, Shengjie Ling
Summary: This study utilized a bio-inspired fibrous artificial tendon (FAT) to repair Achilles tendon defects. The FAT exhibited a highly ordered molecular and nanofibrillar architecture similar to tendons, and promoted the expression of tendon-related genes. These findings suggest the potential clinical applications of FAT.
Review
Multidisciplinary Sciences
Hiroaki Yamanaka, Shigeru Kondo
Summary: Cells exhibit different forms of chirality, such as left-right asymmetry, unidirectional rotation, or spiral movements. These cells demonstrate inherent cell chirality, but the exact function of this phenomenon remains unclear.
Article
Endocrinology & Metabolism
John Garcia, Spenser S. Smith, Sangita Karki, Hicham Drissi, Henry H. Hrdlicka, Daniel W. Youngstrom, Anne M. Delany
Summary: miR-433-3p is a negative regulator of bone formation through various key signaling pathways such as PTH, MAPK, Wnt, and endogenous glucocorticoids, targeting multiple genes including Crebl, Hsd11b1, and Rspo3 to inhibit osteoblast activity and Wnt signaling pathway, reducing bone mass. In in vivo experiments, inhibitors of miR-433-3p can increase trabecular bone volume in mice, indicating its negative regulatory role in bone formation.
JOURNAL OF BONE AND MINERAL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Naohiro Tachibana, Ryota Chijimatsu, Hiroyuki Okada, Takeshi Oichi, Yuki Taniguchi, Yuji Maenohara, Junya Miyahara, Hisatoshi Ishikura, Yasuhide Iwanaga, Yusuke Arino, Kosei Nagata, Hideki Nakamoto, So Kato, Toru Doi, Yoshitaka Matsubayashi, Yasushi Oshima, Asuka Terashima, Yasunori Omata, Fumiko Yano, Shingo Maeda, Shiro Ikegawa, Masahide Seki, Yutaka Suzuki, Sakae Tanaka, Taku Saito
Summary: This study identified that R-spondin 2 (RSPO2) is specifically expressed in a distinct Prg4(+) tendon stem/progenitor cell (TSPC) cluster and suppresses ectopic ossification via chondrogenic differentiation suppression. RSPO2 expression levels were lower in patients with ossification of the posterior longitudinal ligament compared to spondylosis patients, and RSPO2 protein suppressed chondrogenic differentiation in human ligament cells.
Article
Geosciences, Multidisciplinary
Lee Tiszenkel, Shan-Hu Lee
Summary: New particle formation (NPF) occurs in various locations, but not in forests dominated by isoprene. Recent laboratory studies show contradictory theories regarding the role of isoprene in biogenic NPF. To reconcile these discrepancies, flow tube experiments were conducted and it was found that isoprene can either suppress or enhance biogenic NPF depending on the carbon ratios and oxidation regimes, demonstrating the synergetic effects of isoprene and HOx on NPF.
GEOPHYSICAL RESEARCH LETTERS
(2023)
Article
Rheumatology
Nuh Atas, Bagdagul Cakir, Fatih Bakir, Murat Ucar, Hasan Satis, Gizem Tugce Guz, Kubra Durmus Demirel, Hakan Babaoglu, Reyhan Bilici Salman, Aslihan Avanoglu Guler, Hazan Karadeniz, Seminur Haznedarogu, Berna Goker, Mehmet Akif Ozturk, Abdurrahman Tufan
Summary: This study aimed to investigate the impact of anti-TNFs on patients with axSpA and found that many inflammatory cytokines increase after anti-TNF treatment, while noggin levels are not affected.
CLINICAL RHEUMATOLOGY
(2022)
Article
Developmental Biology
Yoshihiro Morishita, Atsushi Kuroiwa, Takayuki Suzuki
Review
Cell Biology
Yoshiyuki Matsubara, Atsushi Kuroiwa, Takayuki Suzuki
DEVELOPMENT GROWTH & DIFFERENTIATION
(2016)
Article
Cell Biology
Leonardo Beccari, Nayuta Yakushiji-Kaminatsui, Joost M. Woltering, Anamaria Necsulea, Nicolas Lonfat, Eddie Rodriguez-Carballo, Benedicte Mascrez, Shiori Yamamoto, Atsushi Kuroiwa, Denis Duboule
GENES & DEVELOPMENT
(2016)
Meeting Abstract
Developmental Biology
Machiko Teramoto, Ryo Sugawara, Yasuo Ishii, Atsushi Kuroiwa, Hisato Kondoh
MECHANISMS OF DEVELOPMENT
(2017)
Article
Ecology
Yoshiyuki Matsubara, Tatsuya Hirasawa, Shiro Egawa, Ayumi Hattori, Takaya Suganuma, Yuhei Kohara, Tatsuya Nagai, Koji Tamura, Shigeru Kuratani, Atsushi Kuroiwa, Takayuki Suzuki
NATURE ECOLOGY & EVOLUTION
(2017)
Article
Cell Biology
Yoshiyuki Matsubara, Atsushi Sakai, Atsushi Kuroiwa, Takayuki Suzuki
DEVELOPMENT GROWTH & DIFFERENTIATION
(2014)
Meeting Abstract
Developmental Biology
Yutaka Kikuchi, Akio Yoshizawa, Yoshinari Nakahara, Toshiaki Izawa, Tohru Ishitani, Makiko Tsutsumi, Atsushi Kuroiwa, Motoyuki Itoh
DEVELOPMENTAL BIOLOGY
(2010)
Article
Developmental Biology
Yo-ichi Yamamoto-Shiraishi, Hiroaki Higuchi, Shigeki Yamamoto, Mie Hirano, Atsushi Kuroiwa
DEVELOPMENTAL BIOLOGY
(2014)
Article
Cell Biology
Akio Yoshizawa, Yoshinari Nakahara, Toshiaki Izawa, Tohru Ishitani, Makiko Tsutsumi, Atsushi Kuroiwa, Motoyuki Itoh, Yutaka Kikuchi
Article
Genetics & Heredity
Masayo Harada, Hirotaka Murakami, Akihiko Okawa, Noriaki Okimoto, Shuichi Hiraoka, Taka Nakahara, Ryogo Akasaka, Yo-ichi Shiraishi, Noriyuki Futatsugi, Yoko Mizutani-Koseki, Atsushi Kuroiwa, Mikako Shirouzu, Shigeyuki Yokoyama, Makoto Taiji, Sachiko Iseki, David M. Ornitz, Haruhiko Koseki
Article
Multidisciplinary Sciences
Shunya Hozumi, Ryo Hirabayashi, Akio Yoshizawa, Mitsuko Ogata, Tohru Ishitani, Makiko Tsutsumi, Atsushi Kuroiwa, Motoyuki Itoh, Yutaka Kikuchi
Article
Multidisciplinary Sciences
Yusuke Watanabe, Stephane Zaffran, Atsushi Kuroiwa, Hiroaki Higuchi, Toshihiko Ogura, Richard P. Harvey, Robert G. Kelly, Margaret Buckingham
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2012)
Article
Multidisciplinary Sciences
Tetsuaki Kimura, Yusuke Nagao, Hisashi Hashimoto, Yo-ichi Yamamoto-Shiraishi, Shiori Yamamoto, Taijiro Yabe, Shinji Takada, Masato Kinoshita, Atsushi Kuroiwa, Kiyoshi Naruse
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Cell Biology
Yoshiyuki Matsubara, Mikiharu Nakano, Kazuki Kawamura, Masaoki Tsudzuki, Jun-Ichi Funahashi, Kiyokazu Agata, Yoichi Matsuda, Atsushi Kuroiwa, Takayuki Suzuki
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2016)
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)