Article
Biology
Camilo Riquelme-Guzman, Stephanie L. Tsai, Karen Carreon Paz, Congtin Nguyen, David Oriola, Maritta Schuez, Jan Brugues, Joshua D. Currie, Tatiana Sandoval-Guzman
Summary: Early events during axolotl limb regeneration, including immune response and the formation of a wound epithelium, are crucial for the successful integration and regeneration of skeletal tissue. Osteoclasts, the cells responsible for bone resorption, play an active and regulated role in this process. The wound epithelium is identified as a regulator of skeletal resorption, potentially releasing signals involved in the recruitment and differentiation of osteoclasts. The correlation between resorption and blastema formation highlights the importance of resorption in skeletal regeneration.
Article
Biology
Kaylee M. Wells, Kristina Kelley, Mary Baumel, Warren A. Vieira, Catherine D. McCusker, K. VijayRaghavan
Summary: Research shows that nerves play a crucial role in determining the size of regenerating limbs in axolotls, with the number of nerves connected to the central nervous system controlling regeneration. It was also found that the mechanism regulating limb size may not be exclusive to axolotls, and further studies are needed to investigate if other animals use similar mechanisms.
Article
Anatomy & Morphology
Sakiya Yamamoto, Rena Kashimoto, Saya Furukawa, Ayaka Ohashi, Akira Satoh
Summary: Axolotls have the ability to regenerate their limbs, and in this study, the authors focused on understanding the reactivation process of the developmental gene Lmx1b during limb regeneration. They found that Lmx1b expression is maintained in the dorsal skin before amputation and is activated after amputation. They also discovered that only cells located in the dorsal side prior to limb amputation can reactivate Lmx1b after amputation, and this reactivation is achieved by the presence of nerves. The nerve factors BMP2 + FGF2 + FGF8 consistently reactivate Lmx1b when applied to the dorsal skin. These findings highlight the importance of nerves in amphibian limb regeneration, both in initiating limb regeneration and reactivating position-specific gene expression.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Cell Biology
Alexander M. Lovely, Timothy J. Duerr, Qingchao Qiu, Santiago Galvan, S. Randal Voss, James R. Monaghan
Summary: The study shows that the Wnt signaling pathway plays a similar role in both limb development and limb regeneration in axolotl salamanders. This provides new insights into the interaction of Wnt signaling with other signaling pathways during salamander limb development and regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biology
Giacomo L. Glotzer, Pietro Tardivo, Elly M. Tanaka
Summary: The expression of fibroblast growth factors (Fgf) ligands in the Apical Ectodermal Ridge (AER) is a conserved feature of limb development in vertebrates. However, the axolotl, a salamander species, has a unique mesenchymal expression of key Fgf ligands in its developing and regenerating limb. This study investigates the hypothesis that alterations in transcription factors and Wnt signaling during evolution have resulted in this unique expression pattern in the axolotl. The results suggest that a shift in tissue responsiveness to canonical Wnt signaling from epidermis to mesenchyme is one contributing factor to the mesenchymal expression of Fgf ligands in the axolotl.
Article
Multidisciplinary Sciences
Warren A. Vieira, Shira Goren, Catherine D. McCusker
Summary: The Mexican Axolotl exhibits remarkable regenerative abilities, and in the process of limb regeneration, positional communication between cells occurs in two types: one involving memory retention within connective tissue cells and another involving the transmission of positional cues to other cell types in the regenerate. The extracellular matrix likely plays a role in facilitating the latter type of communication during limb regeneration.
Article
Genetics & Heredity
Yan Hu, Xiangyu Pan, Yu Shi, Yuanhui Qiu, Liqun Wang, Prayag Murawala, Yanmei Liu, Wanjin Xing, Elly M. Tanaka, Ji-Feng Fei
Summary: Muscle is not essential for guiding other cell types but affects joint formation during limb regeneration in axolotls.
FRONTIERS IN GENETICS
(2022)
Article
Cell Biology
Trey Polvadore, Malcolm Maden
Summary: This study reveals the impact of retinoic acid treatment on the control of positional information during axolotl limb regeneration. Through RNA sequencing, the genes interacting with RAR alpha were identified, shedding light on the mechanism of action for future research.
Article
Cell Biology
Mu-Hui Wang, Chia-Lang Hsu, Cheng-Han Wu, Ling-Ling Chiou, Yi-Tzang Tsai, Hsuan-Shu Lee, Shau-Ping Lin
Summary: The study found that nerve-mediated repressive epigenetic modulator HDAC1 expression regulates gene expression at different time points during axolotl limb regeneration, affecting the regeneration process. HDAC1 activity is required to inhibit the premature elevation of genes related to tissue development, differentiation, and morphogenesis. WNT pathway-associated genes are also prematurely activated under HDAC1 inhibition.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Developmental Biology
Sadik Bay, Gurkan Ozturk, Nesrin Emekli, Turan Demircan
Summary: The Hippo pathway is crucial for various cellular processes, including differentiation, regeneration, cell migration, organ growth, apoptosis, and cell cycle. YAP1, a transcription co-regulator component of the Hippo pathway, is involved in the transcription of genes related to cell proliferation, migration, differentiation, and apoptosis suppression. However, the role of YAP1 in epimorphic regeneration is not fully understood. This study investigated the function of Yap1 in the early blastema stage of limb regeneration in axolotl, providing new insights into the roles of the Hippo pathway in complex structure regeneration.
DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Warren A. Vieira, Michael Raymond, Kristina Kelley, Matthew A. Cherubino, Hande Sahin, Catherine D. McCusker
Summary: This study investigates the failure of integration in the regeneration of limb tissues in the Mexican axolotl, finding significant differences in the complexity of limb structures and gene expression. They also discover that anterior positional information is more abundant at the limb base, and that anterior patterning genes are more highly expressed in proximally located blastemas compared to blastemas in the more distal regions of the limb. These findings provide valuable insights into the underlying causes of integration failure and the distribution of positional identities in the mature limb.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Hao Li, Karin Staxaeng, Monika Hodik, Karl-Gunnar Melkersson, Mathias Rask-Andersen, Helge Rask-Andersen
Summary: The study found that natural hair cell regeneration may occur in the auditory organ of crocodiles. Supporting cells can transdifferentiate into hair cells, and intercellular signaling exists. Crocodiles seem to continuously restore and sculpt their tectorial membrane throughout their lives.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Florian Rouaud, Isabelle Mean, Sandra Citi
Summary: The study investigated the expression and localization of ACE2 in epithelial tissues and cultured cells, as well as its association with transmembrane and tight junction proteins. The findings identify ACE2, ADAM17, and CD9 as new epithelial junctional transmembrane proteins and suggest that cytokine-enhanced endocytic internalization may promote coronavirus entry by facilitating the internalization of junction-associated protein complexes containing ACE2.
Article
Cell Biology
Zheng Zhang, Feng Zhang, Ashley Kuenzi Davis, Mei Xin, Gerd Walz, Weidong Tian, Yi Zheng
Summary: Epithelial polarity is controlled by CDC42 and Scribble/PAR. Loss of CDC42 leads to hyperproliferation of transit amplifying cells and disruption of epithelial polarity. Activation of YAP/TAZ and mTOR signaling pathways is associated with CDC42 loss.
Article
Multidisciplinary Sciences
Arsalan Ul Haq, Luca Montaina, Francesca Pescosolido, Felicia Carotenuto, Federica Trovalusci, Fabio De Matteis, Emanuela Tamburri, Paolo Di Nardo
Summary: Electrically conductive scaffolds with directional alignment of muscle fibers were fabricated using 3D printing micro-stereolithography. The conductive ink used for printing was made by blending polyethylene glycol diacrylate, Irgacure 819, curcumin, and polyaniline. The obtained scaffolds demonstrated well defined morphology and semi-conducting properties. Cyclic voltammetry analyses detected electroactivity and highlighted ionic diffusion between the polymer and the electrolyte solution. The conductive scaffolds displayed tunable conductivity and provided an optimal environment for cultured mouse cardiac progenitor cells.
SCIENTIFIC REPORTS
(2023)
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)