Review
Biochemistry & Molecular Biology
Enoli De Silva, Felix Hong, Herve Falet, Hugh Kim
Summary: This mini-review summarizes how FLNA transduces critical cell signals to the platelet cytoskeleton.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Immunology
Nayoung Kim, Eunbi Yi, Soon Jae Kwon, Hyo Jin Park, Hyung-Joon Kwon, Hun Sik Kim
Summary: This study reveals the important role of the actin-binding protein filamin A (FLNa) in regulating the cytotoxicity and cytokine production of NK cells. FLNa is recruited to the NK cell lytic synapse and regulates conjugate formation, filamentous actin accumulation, and cytotoxic degranulation. The loss of FLNa enhances the activation signals and delayed down-modulation of the NKG2D receptor in NK cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Kangxi Zhou, Yue Xia, Mengnan Yang, Weiling Xiao, Lili Zhao, Renping Hu, Khan Muhammad Shoaib, Rong Yan, Kesheng Dai
Summary: Actin polymerization plays a role in regulating GPIb alpha shedding by activating calpain and hydrolyzing filamin A, which in turn affects platelet function. These findings suggest a novel strategy to negatively regulate platelet function through ADAM17-mediated GPIb alpha shedding.
Article
Cell Biology
Shiri Avivi Kela, Kriti Sethi, Pei Yi Tan, Danesha Suresh, Hui Ting Ong, Perla G. Castaneda, Mustafi R. Amin, Tal Laviv, Erin J. Cram, Jan Faix, Ronen Zaidel-Bar
Summary: This study identified a novel mechanotransduction pathway in the C. elegans spermatheca, which involves tension-dependent localization of a RhoGEF and a positive feedback loop for RHO-1-MyosinII activation. It provides insights into how epithelial tubes can sense and adjust their contractility in response to internal pressure.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Developmental Biology
Jing Dai, Yongzhe Chen, Qi Li, Tianlei Zhang, Qinwei Zhou, Fei Gong, Guangxiu Lu, Wei Zheng, Ge Lin
Summary: Teratozoospermia caused by ACTL7A gene mutation in mice affects sperm activity and fertilization ability, leading to total fertilization failure. Calcium ionophore exposure can successfully overcome fertilization failure caused by acrosomal abnormalities.
MOLECULAR HUMAN REPRODUCTION
(2022)
Article
Hematology
Lamia Lamrani, Frederic Adam, Christelle Soukaseum, Cecile Denis, Hana Raslova, Jean-Philippe Rosa, Marijke Bryckaert
Summary: This study evaluates the role of filamin A (FLNa) subdomains in regulating integrin alpha IIb beta 3 signaling. The results show that FLNa-actin and FLNa-beta 3 interactions negatively regulate alpha IIb beta 3 activation, while FLNa dimerization domain negatively regulates alpha IIb beta 3 outside-in signaling.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Endocrinology & Metabolism
Jackson Sapuleni, Magdalena Szymanska, Rina Meidan
Summary: By activating SIRT1, key ovulatory and angiogenic genes are upregulated in human granulosa-lutein cells, but this also leads to the advancement of cell death pathways. These effects appear to be regulated by cAMP.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ana Filipa Sobral, Fung-Yi Chan, Michael J. Norman, Daniel S. Osorio, Ana Beatriz Dias, Vanessa Ferreira, Daniel J. Barbosa, Dhanya Cheerambathur, Reto Gassmann, Julio Monti Belmonte, Ana Xavier Carvalho
Summary: The study demonstrates a synergy between the small rigid crosslinker plastin and the large flexible crosslinker spectrin in the C. elegans embryo, showing that their combined action stabilizes the actomyosin network in cytokinesis. Co-inhibition of plastin and beta H-spectrin results in cytokinesis failure, highlighting the importance of crosslinker interplay for network integrity.
Article
Biochemistry & Molecular Biology
Myun Soo Kim, Dongmin Park, Sora Lee, Sunyoung Park, Kyung Eun Kim, Tae Sung Kim, Hyun Jeong Park, Daeho Cho
Summary: The erythroid differentiation regulator 1 (Erdr1) enhances TCR signaling strength in CD4 T cells by promoting the activation and proliferation of T cells, amplifying Ca2+ influx and phosphorylation of PLC gamma 1, and promoting NFAT1 translocation into nuclei in the presence of TCR stimulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Sonali Munshaw, Susann Bruche, Andia N. Redpath, Alisha Jones, Jyoti Patel, Karina N. Dube, Regent Lee, Svenja S. Hester, Rachel Davies, Giles Neal, Ashok Handa, Michael Sattler, Roman Fischer, Keith M. Channon, Nicola Smart
Summary: The study identifies Thymosin beta 4 (T beta 4) as a key regulator of LRP1 for maintaining vascular health, and highlights the role of their interaction in modulating the phenotype of vascular smooth muscle cells (VSMCs), providing insights into the mechanisms underlying aortic disease progression.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Cell Biology
Katsueki Ogiwara, Chika Fujimori, Takayuki Takahashi
Summary: The prostaglandin E2/Ptger4b receptor system plays a role in ovulation and actin cytoskeleton rearrangement in medaka. In this study, it was found that treating preovulatory follicles with Ptger4b antagonist, Rho inhibitor, or Rock inhibitor inhibited follicle ovulation and actin cytoskeleton rearrangement. The activation of Rhoa-c and Rock1 was detected before ovulation, and their activation and cytoskeleton rearrangement were inhibited by Ptger4b antagonist. PGE2-induced actin cytoskeleton rearrangement was not observed in cells deficient in Ptger4b, Rhoa-c, or Rock1.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2023)
Article
Biology
Jeong Min Chung, Osamu Sato, Reiko Ikebe, Sangmin Lee, Mitsuo Ikebe, Hyun Suk Jung
Summary: This study investigated the mechanism by which fascin-1 induces actin bundling and its localization along actin filaments. The researchers found that the N-terminal region of fascin-1 is responsible for actin bundling, while the C-terminal region is important for fascin-1 dimerization. Furthermore, they discovered that the intermolecular interactions of the C-terminal region are essential for actin bundling.
Article
Biochemistry & Molecular Biology
Robyn T. Rebbeck, Anna K. Andrick, Sarah A. Denha, Bengt Svensson, Piyali Guhathakurta, David D. Thomas, Thomas S. Hays, Adam W. Avery
Summary: This study established a high-throughput screening method for discovering potential disrupters of the interaction between mutant beta-III-spectrin ABD and actin in live cells, showing its effectiveness.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Suniti Misra, Shibnath Ghatak, Ricardo A. Moreno-Rodriguez, Russell A. Norris, Vincent C. Hascall, Roger R. Markwald
Summary: The study reveals the crucial interaction between extracellular matrix protein periostin and cytoskeletal protein filamin A in promoting the maturation and remodeling of heart valves. This bidirectional interaction between activated FLNA and Pak1 plays a vital role in actin cytoskeletal reorganization and the differentiation of immature VICs into mature valve leaflets. Patients with specific point mutations in the filamin A gene exhibit impaired response to periostin signaling, leading to inadequate valve leaflet formation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Gilberto Garcia, Raz Bar-Ziv, Maxim Averbukh, Nirmalya Dasgupta, Naibedya Dutta, Hanlin Zhang, Wudi Fan, Darius Moaddeli, C. Kimberly Tsui, Toni Castro Torres, Athena Alcala, Erica A. A. Moehle, Sally Hoang, Ophir Shalem, Peter D. D. Adams, Max A. A. Thorwald, Ryo Higuchi-Sanabria
Summary: The actin cytoskeleton plays a crucial role in regulating cellular pathways and its deterioration can lead to aging and stress-related problems. This study identifies the bromodomain protein BET-1 as a key regulator of actin function and longevity. Overexpression of bet-1 preserves actin function and promotes lifespan and healthspan in C. elegans.
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)