Article
Cell Biology
Raquel Perez-Palacios, Maria Climent, Javier Santiago-Arcos, Sofia Macias-Redondo, Martin Klar, Pedro Muniesa, Jon Schoorlemmer
Summary: YY2, a mammalian-specific transcription factor, is expressed during mouse preimplantation development but its presence seems to be dispensable until the blastocyst stage in vitro. Depletion of Yy2 in mESCs results in increased levels of Zscan4 expression but does not affect the expression of TEs or other pluripotency markers.
Article
Engineering, Biomedical
Lakshmi Kavitha Sthanam, Tanusri Roy, Sejal Patwardhan, Avi Shukla, Shipra Sharma, Pradip Shinde, Hanuman Tulasiram Kale, P. Chandra Shekar, Kiran Kondabagil, Shamik Sen
Summary: Stem cell differentiation is influenced by the interaction between cells and their extracellular matrix, with matrix metalloproteinases (MMPs) playing a role in this process. Differentiation of mouse embryonic stem cells (mESCs) on varying matrices led to changes in MMP expression and activity, affecting integrin expression, mechanotransduction pathways, and germ layer-specific markers. Inhibition of MMP activity altered the germ layer commitment of differentiating mESCs, demonstrating the importance of MMPs in regulating stem cell differentiation.
Article
Cell Biology
Masahiro Otsu, Zubair Ahmed, Daniel Fulton
Summary: A simple method was developed to generate immature oligodendrocyte lineage cells from mouse ESC-derived neural stem cells, which showed potential for differentiation into mature OPC under specific conditions. Interestingly, these cells retained multipotentiality and could differentiate into neurons or astrocytes. However, their efficiency in myelinating axons was lower compared to primary OPC.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Jose Manuel Inacio, Joao von Gilsa Lopes, Ana Mafalda Silva, Fernando Cristo, Sara Marques, Matthias E. Futschik, Jose Antonio Belo
Summary: Deciphering the clues of a regenerative mechanism for the mammalian adult heart, particularly focusing on the role of DAND5, could potentially lead to the development of more effective treatment options for heart failure. Further research on the transcriptional profiling sequencing and quantitative RT-PCR assays of DAND5 may provide insights into the generation and expansion of pluripotent stem cell-derived cardiomyocytes systems for clinical applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Yuting Fu, Fangyuan Liu, Shuo Cao, Jia Zhang, Huizhi Wang, Baojiang Wu, Yongli Song, Shuguang Duo, Xihe Li, Siqin Bao
Summary: This study reveals that Bdh2 deficiency has no impact on pluripotency and self-renewal of ESCs, but promotes endoderm differentiation and is essential for maintaining DNA methylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yan Li, Zhiyu Xia, Haihong Yin, Youran Dai, Feixue Li, Jianming Chen, Mengsheng Qiu, Huarong Huang
Summary: A simple and efficient method for inducing mouse embryonic stem cells into primitive endoderm cells was reported in this study, and it was found that the Wnt signaling pathway played a critical role in this process.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
Chika Ogura, Shoko Nishihara
Summary: This study demonstrated the important role of DS in maintaining the undifferentiated state of mESCs and influencing their differentiation direction and Wnt signaling pathway. Knockdown or overexpression experiments targeting the D4ST1 gene revealed the mechanism of DS in mESCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Edward J. Calabrese
Summary: This paper provides a thorough assessment of hormetic dose responses in embryonic stem cells, suggesting that this response may serve as a fundamental and highly conserved evolutionary strategy.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Review
Cell & Tissue Engineering
Sonam Raj, Sushil K. Jaiswal, Melvin L. DePamphilis
Summary: This review article addresses the enigmatic role of the p53 transcription factor and tumor suppressor in programmed cell death during embryonic development. Contradictory results regarding the dependence of pluripotent embryonic stem cells on p53 for cell death are reconciled based on experimental conditions. The review concludes that p53 is not required for mouse embryonic development, but can accelerate programmed cell death in certain conditions. Additionally, DNA damage in differentiated cells induces p53-dependent cell cycle arrest and senescence.
Article
Multidisciplinary Sciences
Nagham Khouri-Farah, Qiuxia Guo, Kerry Morgan, Jihye Shin, James Y. H. Li
Summary: Recent studies using single-cell RNA-sequencing have identified cellular heterogeneity in the developing mammalian cerebellum, but the regulatory logic behind this diversity remains unclear. Researchers integrated single-cell RNA and ATAC analyses to understand the developmental trajectories of cerebellar progenitors and identified potential regulatory elements controlling cell state transitions. Using gene regulatory network analysis, they uncovered the molecular control of a specific progenitor zone and demonstrated its association with a common cerebellar birth defect in humans.
Article
Cell Biology
Jingsheng Li, Chunhong Dai, Wenyan Xie, Heyao Zhang, Xin Huang, Constantinos Chronis, Ying Ye, Wensheng Zhang
Summary: This study developed a one-step strategy to efficiently target and suppress endogenous pluripotent genes in mouse ESCs and replace their expression with AID-fused transgenes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Genetics & Heredity
Hao Li, Peter Hohenstein, Satu Kuure
Summary: The adult mammalian kidney lacks stem cells for regeneration, unlike the embryonic kidney which contains lineage-specific stem cells giving rise to various kidney components; current research focuses on these embryonic progenitor cells which can shed light on the formation of Wilms tumor, a pediatric renal cancer.
Article
Environmental Sciences
Jin Liu, Lingfang Li, Jianlin Zhu, Lingfeng Luo, Yuchen Li, Chenyun Zhang, Wenchang Zhang
Summary: Cadmium (Cd) can interrupt the differentiation of mouse embryonic stem cells into ovarian granulosa cells, and this effect may involve epigenetic mechanisms.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Multidisciplinary Sciences
Shao-Hui Pan, Ning Zhao, Xiang Feng, Ying Jie, Zi-Bing Jin
Summary: The study developed a stepwise strategy to generate chemically induced neural crest cells (ciNCCs) and chemically induced corneal endothelial cells (ciCECs) from mouse fibroblasts using defined small molecules. The resulting ciCECs exhibited consistent gene expression profiles and self-renewal capacity to primary CECs, and could be cultured for as long as 30 passages while retaining their features. Transplantation of these ciCECs into an animal model reversed corneal opacity, providing an alternative cell source for regeneration of corneal endothelia and tissues derived from neural crest.
Article
Environmental Sciences
Jingyan Li, Huimin Weng, Shuang Liu, Fan Li, Ke Xu, Shan Wen, Xi Chen, Chang Li, Yongmei Nie, Bin Liao, Jianming Wu, Fahsai Kantawong, Xiang Xie, Fengxu Yu, Guang Li
Summary: This study found the presence of micro- and nanoplastics in human blood and placentas, indicating embryonic exposure to these particles. Furthermore, the exposure to polystyrene nanoplastics (PS-NPs) was found to negatively impact the cardiac differentiation of human embryonic stem cells (hESCs), leading to immature cardiomyocytes and impaired contractility. Mechanistically, PS-NPs induced mitochondrial oxidative stress, activated P38/Erk MAPK signaling pathway, and blocked autophagy flux, reducing the pluripotency of hESCs. Consistent results were observed in zebrafish embryo models, with reduced cardiac contraction and blood flow after exposure to PS-NPs.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)
