Article
Multidisciplinary Sciences
Karin D. Prummel, Helena L. Crowell, Susan Nieuwenhuize, Eline C. Brombacher, Stephan Daetwyler, Charlotte Soneson, Jelena Kresoja-Rakic, Manuel Ronner, Agnese Kocere, Alexander Ernst, Zahra Labbaf, David E. Clouthier, Anthony B. Firulli, Hector Sanchez-Iranzo, Sundar R. Naganathan, Rebecca O'Rourke, Erez Raz, Nadia Mercader, Alexa Burger, Emanuela Felley-Bosco, Jan Huisken, Mark D. Robinson, Christian Mosimann
Summary: This study uncovers the early origins of mesothelium progenitor cells in zebrafish, linking the function of the Hand2 gene to mesothelium formation and the re-activation of mesothelioma tumors.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Tamas Kovacs, Viktoria Halasy, Csongor Petho, Emoke Szocs, Adam Soos, David Dora, Pascal de Santa Barbara, Sandrine Faure, Rhian Stavely, Allan M. Goldstein, Nandor Nagy, Nicolas Pilon
Summary: The enteric nervous system (ENS) is derived from vagal neural crest cells and plays a crucial role in gastrointestinal development. This study shows that the avian ceca, located at the junction of the midgut and hindgut, are necessary for hindgut ENS development. It also highlights the importance of bone morphogenetic protein-4 (BMP4) and glial cell line-derived neurotrophic factor (GDNF) signaling pathways in the migration of enteric neural crest-derived cells (ENCDC) and the formation of enteric ganglia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Verena Pawolski, Mirko H. H. Schmidt
Summary: The enteric nervous system (ENS) is a major part of the peripheral nervous system, receiving increasing attention in recent years. ENS development is mainly derived from vagal and sacral neural crest cell portions, regulated by various signaling molecules. The fully formed adult ENS may react to injury with neurogenesis and gliogenesis, but the origin of differentiating cells remains under debate.
Article
Cell Biology
Aubrey G. A. Howard, Aaron C. Nguyen, Joshua Tworig, Priya Ravisankar, Eileen W. Singleton, Can Li, Grayson Kotzur, Joshua S. Waxman, Rosa A. Uribe
Summary: Neural crest cells (NCCs) are migratory and multipotent stem cells essential to vertebrate embryonic development. This study identifies the gene Hoxb5b as a regulator of NCC development, demonstrating that elevated Hoxb5b levels promote expansion of zebrafish NCCs and specifically expand expression domains of vagal NCC markers. The study also shows that early increase in vagal NCCs leads to supernumerary enteric neural progenitors, but these fail to properly expand into enterically fated neurons in the gut tissue.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Neurosciences
Jean Marie Delalande, Nandor Nagy, Conor J. McCann, Dipa Natarajan, Julie E. Cooper, Gabriela Carreno, David Dora, Alison Campbell, Nicole Laurent, Polychronis Kemos, Sophie Thomas, Caroline Alby, Tania Attie-Bitach, Stanislas Lyonnet, Malcolm P. Logan, Allan M. Goldstein, Megan G. Davey, Robert M. W. Hofstra, Nikhil Thapar, Alan J. Burns
Summary: The study revealed the important role of TALPID3 in GI and ENS development, with its absence leading to abnormal GI tract development in chicken embryos. ENS formation was found not to depend on TALPID3 but on environmental cues. The presence of KIAA0586 mutation in human fetal tissues showed similar findings to animal models, highlighting the essential role of TALPID3 in human GI tract development and patterning.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Developmental Biology
Ezra Lencer, Addison Rains, Erin Binne, Rytis Prekeris, Kristin B. Artinger
Summary: The transmembrane proteins cdon and boc are involved in regulating hedgehog signaling and have additional roles in axon guidance and neural crest cell migration. The study investigates the function of cdon and boc in zebrafish neural crest cell migration using mutants and finds disruption of migration in double cdon;boc mutants. The study also reveals defects in slow-twitch muscle cell differentiation and extracellular matrix loss, suggesting that neural crest defects may be secondary to mesoderm development defects.
Review
Cell Biology
Werend Boesmans, Amelia Nash, Kinga R. Tasnady, Wendy Yang, Lincon A. Stamp, Marlene M. Hao
Summary: Enteric glia are support cells of the enteric nervous system that play a vital role in regulating gut functions and maintaining homeostasis. Their plasticity and heterogeneity make them neural precursors with the potential for neurogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Rhian Stavely, Sukhada Bhave, Wing Lam N. Ho, Minhal Ahmed, Weikang Pan, Ahmed A. Rahman, Jessica Ulloa, Nicole Bousquet, Meredith Omer, Richard Guyer, Nandor Nagy, Allan M. Goldstein, Ryo Hotta
Summary: The interaction between ENSCs and EMCs plays a crucial role in the normal development of the enteric nervous system, with potential implications for treating Hirschsprung disease. EMCs enhance the expansion and neurosphere-forming ability of ENCDCs through paracrine factors, leading to functional neurons and successful transplantation in the HSCR model.
Review
Biochemistry & Molecular Biology
Zhixin Li, Elly Sau-Wai Ngan
Summary: With the rapid development of single-cell sequencing technologies, the use of single-cell multimodal sequencing has become a powerful strategy in discovering rare cells and understanding biological processes. This article summarizes recent findings in single-cell multimodal sequencing, particularly focusing on single-cell RNA sequencing (scRNA-seq) in the enteric nervous system (ENS). The studies have revealed the heterogeneity of ENS cells at different developmental stages and identified novel cell types. The application of single-cell multimodal omics techniques in profiling disease mechanisms and cellular drug responses is expected to revolutionize biomedical research.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
Y. Wu, H. Kurosaka, Q. Wang, T. Inubushi, K. Nakatsugawa, M. Kikuchi, H. Ohara, T. Tsujimoto, S. Natsuyama, Y. Shida, L. L. Sandell, P. A. Trainor, T. Yamashiro
Summary: Embryonic craniofacial development relies on the coordinated growth and fusion of facial primordia. Rdh10 and RA signaling play a crucial role in orchestrating the normal midfacial development during early embryogenesis. Rdh10 deficiency can lead to midfacial deformities, and misregulation of Shh signaling is a significant factor in the pathogenesis of these defects.
JOURNAL OF DENTAL RESEARCH
(2022)
Article
Developmental Biology
Rhian Stavely, Ryo Hotta, Richard A. Guyer, Nicole Picard, Ahmed A. Rahman, Meredith Omer, Adam Soos, Emoke Szocs, Jessica Mueller, Allan M. Goldstein, Nandor Nagy
Summary: The development of the enteric nervous system is dependent on the colonization of the intestine by enteric neural crest-derived cells (ENCDCs). ENCDCs at the wavefront, which are highly migratory and proliferative, exhibit distinct molecular characteristics compared to trailing ENCDCs. These wavefront ENCDCs show altered expression of genes associated with migration and lack expression of genes related to neuronal or glial maturation.
Review
Biochemistry & Molecular Biology
Richard A. Guyer, Jessica L. Mueller, Allan M. Goldstein
Summary: With the advancement of technology, single-cell sequencing has become a common tool to analyze cellular diversity and reveal cellular diversity within organs. The application of these methods in the study of the enteric nervous system has provided abundant data and biological insights. As technology continues to evolve, these methods may become commonplace in enteric nervous system research and diagnostic testing.
Article
Genetics & Heredity
Christina A. Kearns, Macie Walker, Andrew M. Ravanelli, Kayt Scott, Madeline R. Arzbecker, Bruce Appel
Summary: The vertebrate neural tube axis is patterned by a gradient of Shh signaling, with the pMN domain progenitors expressing Olig2 and producing motor neurons and oligodendrocytes. A mutant allele of the boc gene in zebrafish disrupts oligodendrocyte development by affecting the maintenance of pMN progenitors, demonstrating the importance of boc in shaping the Shh signaling gradient for neural tube patterning.
Article
Developmental Biology
Nandor Nagy, Tamas Kovacs, Rhian Stavely, Viktoria Halasy, Adam Soos, Emoke Szocs, Ryo Hotta, Hannah Graham, Allan M. Goldstein
Summary: The migration and proliferation of ENCCs are crucial for ENS formation, with the ceca playing an important role in ENS development. The non-canonical Wnt signaling pathway regulates ENCC differentiation by inhibiting enteric neuronal differentiation.
Article
Biochemistry & Molecular Biology
Rodolphe Soret, Nejia Lassoued, Gregoire Bonnamour, Guillaume Bernas, Aurelie Barbe, Melanie Pelletier, Manon Aichi, Nicolas Pilon
Summary: Hirschsprung disease is a congenital malformation associated with the absence of ganglia in the enteric nervous system. Genetic background can influence the severity and response to treatments of the disease, as shown in Holstein mice. The enteric nervous system appears to be more sensitive to genetic background changes compared to other neural crest derivatives.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)
