Article
Fisheries
Xiaoqing Xiong, Hong Yang, Chunhua Ding, Beibei Qin, Yadong Deng, Liming Xiong, Xiaoyan Liu, Yaoguo Li, Tiaoyi Xiao, Zhao Lv
Summary: In this study, a newly discovered integrin αvβ3 in grass carp was found to interact with viral proteins and mediate antiviral immune response. Furthermore, the expression of this integrin was significantly upregulated in various tissues of grass carp after infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Hongyan Yu, Xuewen Jia, Yifan Pang, Huiqin Niu, Biao Du, Xiaoyan Xu, Jiale Li
Summary: In this study, it was found that the TBK1 gene in grass carp could be upregulated by bacterial infection. Overexpression of TBK1 could reduce the number of adhesive bacteria in cells and promote cellular migration, proliferation, vitality, and anti-apoptosis ability. TBK1 could activate the NF-kappa B signaling pathway and induce autophagy in cells. These findings provide important information about the defense and immune mechanisms used by grass carp against pathogens.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biology
Beibei Qin, Tiaoyi Xiao, Chunhua Ding, Yadong Deng, Zhao Lv, Jianming Su
Summary: This study identified 42 CiTRIMs in grass carp, with some TRIMs-B30.2 genes associated with the type I interferon response during GCRV infection, providing potential antiviral immune molecule markers for grass carp.
Article
Fisheries
Pengfei Chu, Yuchao Zhu, Luqian Xu, Xiangyu Yao, Yan Liang, Xiaojun Zhang
Summary: This study identified and analyzed the functional role of ATG4C, a protein involved in autophagy, in grass carp. ATG4C was found to be widely distributed in grass carp tissues, with high expression in the intestine. It showed a strong response in immune tissues after infection with grass carp reovirus (GCRV), indicating its involvement in the immune response. Additionally, the study confirmed the induction of autophagy and apoptosis by GCRV invasion, and revealed that ATG4C positively regulated autophagy while restricting apoptosis, leading to inhibition of GCRV replication.
Article
Fisheries
Zhensheng Wang, Chuang Xu, Yanqi Zhang, Xingchen Huo, Jianguo Su
Summary: The study confirms that the use of nanoparticles CMCS-20a can efficiently prevent grass carp reovirus (GCRV) infection, enhance the immunity of fish, and alleviate tissue damage caused by the virus.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Microbiology
Fanshu Xiao, Lanjie Liao, Qiaoqing Xu, Zhili He, Tiaoyi Xiao, Jianjun Wang, Jie Huang, Yuhe Yu, Bo Wu, Qingyun Yan
Summary: This study observed a dysbiosis of gut microbiota in grass carp infected with the GCRV virus, with an overabundance of Cetobacterium somerae triggering exacerbated inflammatory responses through NLRs, TLRs, and LPS pathways.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Immunology
Changyong Mu, Qiwang Zhong, Yan Meng, Yong Zhou, Nan Jiang, Wenzhi Liu, Yiqun Li, Mingyang Xue, Lingbing Zeng, Vikram N. Vakharia, Yuding Fan
Summary: In this study, a recombinant baculovirus expressing VP35-VP4 proteins from GCRV was generated and used to vaccinate grass carp through oral administration. The results showed that the vaccine induced both non-specific and specific immune responses, providing protection against GCRV infection with a relative percent survival rate of 56%. This study suggests that the silkworm pupae powder could be used as an effective oral vaccine for grass carp.
Article
Fisheries
Fanbin Zhan, Yanan Li, Fei Shi, Zhijie Lu, Minxuan Yang, Qingqing Li, Li Lin, Zhendong Qin
Summary: This study identified two forms of TLR5 (TLR5a and TLR5b) in grass carp and investigated their role in innate immunity against flagellate and non-flagellate bacteria. It was found that TLR5b had higher expression levels and stronger response to pathogen stimulation compared to TLR5a. The results provide insight into the role of TLR5 in fish infection resistance and contribute to the research on innate immunity in grass carp.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Baohong Xu, Hang Su, Changjun Chen, Qiaolin Liu, Tiaoyi Xiao, Yi Liu, Zhao Lv, Hongquan Wang, Zheyu Wen
Summary: The interaction of C5a and C5aR in grass carp infected with grass carp reovirus promotes the activation of p38 and ERK proteins, leading to increased expression of IL-6 and TNF-alpha and induction of an inflammatory response. These findings provide a theoretical basis for regulating the interaction of C5a and C5aR to alleviate grass carp hemorrhagic diseases.
AQUACULTURE REPORTS
(2023)
Article
Virology
Rui Jiang, Jie Zhang, Zhiwei Liao, Wentao Zhu, Hang Su, Yongan Zhang, Jianguo Su
Summary: This study investigated the latent infection of GCRV-II in asymptomatic grass carp with GCRV infection or exposure history. It was found that GCRV-II was detectable only in the brain of grass carp during latent infection, unlike the multi-tissue distribution observed in natural infection. Additionally, GCRV-II distribution in grass carp was notably affected by ambient temperature. This study provides insights into the mechanisms of GCRV-II latent infection and reactivation, contributing to the prevention and control of GCRV pandemics.
Article
Immunology
Xudong Hu, Bing Wang, Haohao Feng, Man Zhou, Yusheng Lin, Hong Cao
Summary: Protein phosphatase-1 (PP1) plays an important role in various cell functions, including cell differentiation, development, immune response, and tumorigenesis. This study focused on the role of PPP1R3G in defending against GCRV infection in grass carp, showing that CiPPP1R3G is significantly up-regulated in response to infection and negatively regulates CiIRF3 expression. The findings suggest that CiPPP1R3G functions as a negative regulator in the anti-GCRV immune responses.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Juan Du, Han Xiao, Yanqiu Hu, Zhi Li
Summary: Grass carp is an economically important fish, but the viral hemorrhagic diseases caused by grass carp reovirus (GCRV) can significantly impact its economic yield. This study focused on the role of Cimarch2 in the antiviral innate immune response in grass carp. The results showed that Cimarch2 has high homology in grass carp and other species, and it can regulate interferon activation and promote viral replication through the downregulation of tbk1 via the proteasome pathway. These findings suggest the potential of Cimarch2 in antiviral breeding of grass carp.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Virology
Ling Yang, Jianguo Su
Summary: In this study, it was found that GCRV can only infect leukocytes in vitro, while erythrocytes and thrombocytes are not susceptible. Additionally, the expression levels of immune response-related genes were significantly up-regulated in infected leukocytes.
Article
Fisheries
Minxuan Yang, Zhijie Lu, Fenglin Li, Fei Shi, Fanbin Zhan, Lijuan Zhao, Yanan Li, Jun Li, Li Lin, Zhendong Qin
Summary: The study revealed that fish red blood cells are not only responsible for oxygen transportation, but also potentially play a role in immune response against bacterial infections. After incubation with E. coli, grass carp RBCs showed changes in gene expression, activating various immune responses.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Virology
Wentao Zhu, Meihua Qiao, Meidi Hu, Xingchen Huo, Yongan Zhang, Jianguo Su
Summary: This study found that Type II grass carp reovirus (GCRV-II) rapidly invades grass carp through the nostril, gill, and skin on the head, rather than merely by adhesion. Further investigations revealed that GCRV-II invades grass carp via the nostril-olfactory system-brain axis, and viral replication occurs.
Article
Biochemistry & Molecular Biology
Zhiwei Liao, Chunrong Yang, Rui Jiang, Wentao Zhu, Yongan Zhang, Jianguo Su
Summary: The membrane-bound Toll-like receptor 5 (TLR5) in cyprinid grass carp plays a role in detecting bacterial flagellin and dsRNA analog poly(I:C), regulating interferon response and antiviral defense. Two TLR5 paralogs, CiTLR5a and CiTLR5b, have opposite effects on virus replication. The ectodomain of CiTLR5 is essential for dsRNA-induced interferon signaling, and specific glycans affect dsRNA-IFN signaling but not flagellin-induced NF-kappa B induction. This study reveals a neofunctionalization of TLR5 in cyprinid, bridging viral and bacterial immune responses.
Article
Fisheries
Huabing Wang, Feifei Zheng, Aotian Ouyang, Gailing Yuan, Jianguo Su, Xiaoling Liu
Summary: This study cloned and functionally characterized MaCSF-1 and MaCSF-1R from blunt snout bream, and found that they play crucial roles in macrophage proliferation, phagocytosis, and IL-10 production.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Zhensheng Wang, Chuang Xu, Yanqi Zhang, Xingchen Huo, Jianguo Su
Summary: The study confirms that the use of nanoparticles CMCS-20a can efficiently prevent grass carp reovirus (GCRV) infection, enhance the immunity of fish, and alleviate tissue damage caused by the virus.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Jiao Chang, Wentao Zhu, Xingchen Huo, Meihua Qiao, Chunrong Yang, Yongan Zhang, Jianguo Su
Summary: In this study, a recombinant Lactobacillus casei expressing multiple fragments of the key outer capsid protein of grass carp reovirus (GCRV) was constructed, and an oral microcapsule live vaccine (SCL-56310-500) was prepared. The results showed that this vaccine effectively enhanced the immune response and suppressed GCRV replication in fish, leading to a significantly higher survival rate.
Article
Fisheries
Yanqi Zhang, Jianguo Su
Summary: This review discusses the unique biological effects of IL-2 family cytokines in regulating differentiation, survival, and activation of multiple lymphocyte lineages, as well as their expression profiles, receptors, signaling transductions, and functions in teleosts. The IL-2 family cytokines in teleosts show relatively conserved functions in lymphocyte proliferation, differentiation, development, and inflammation, similar to their counterparts in mammals.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Immunology
Wentao Zhu, Yanqi Zhang, Zhiwei Liao, Xingchen Huo, Chunrong Yang, Yongan Zhang, Jianguo Su
Summary: This study reveals the strong antibacterial activity of type I interferon IFN1 in teleosts, which is mediated by phagocytosis and induction of immune factors and complement components. These findings shed light on the functional role of interferons in bacterial infections.
JOURNAL OF IMMUNOLOGY
(2023)
Article
Fisheries
Weixiang Zhang, Zhenjun Zhao, Jiancheng Zhou, Weicheng Wang, Jianguo Su, Gailing Yuan
Summary: This study evaluates the feasibility and biological activity of carboxymethyl chitosan (CMCS) loaded with Ctenopharyngodon idella interferon-gamma 2 (CiIFN-gamma 2), and examines the resistance to Aeromonas hydrophila infection in grass carp. The results show that CMCS-CiIFN-gamma 2 nanoparticles can enhance the expression of pro-inflammatory cytokines and the effective uptake of the protein, leading to improved immune protection against bacterial diseases in aquaculture.
Article
Fisheries
Qian Liu, Xingchen Huo, Qingqing Tian, Pengxu Wang, Fengxia Zhao, Chunrong Yang, Jianguo Su
Summary: The oral P-MCP-FlaC vaccine effectively controls LMBV infection in largemouth bass, improving the survival rate and up-regulating protective enzyme activity and immune gene expression.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Aotian Ouyang, Mengwei Zhang, Gailing Yuan, Xiaoling Liu, Jianguo Su
Summary: This study reveals that chitooligosaccharide (COS) has the ability to reverse cortisol-induced immunosuppression in fish and enhance the immune activity of macrophages. Oral administration of COS directly absorbed through the intestine significantly improves the innate immunity of blunt snout bream, leading to increased survival and reduced tissue damage. Thus, COS offers potential strategies for immunosuppression prevention and control in fish.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Virology
Wentao Zhu, Meihua Qiao, Meidi Hu, Xingchen Huo, Yongan Zhang, Jianguo Su
Summary: This study found that Type II grass carp reovirus (GCRV-II) rapidly invades grass carp through the nostril, gill, and skin on the head, rather than merely by adhesion. Further investigations revealed that GCRV-II invades grass carp via the nostril-olfactory system-brain axis, and viral replication occurs.
Article
Immunology
Qingqing Tian, Xingchen Huo, Qian Liu, Chunrong Yang, Yongan Zhang, Jianguo Su
Summary: This study developed a prophylactic grass carp reovirus (GCRV) vaccine using virus-like particle (VLP) assembly technology, and the highly immunogenic candidates in the vaccine were VP4, VP56, and VP35. Expression of VP4, VP56, and VP35 in both Escherichia coli and Pichia pastoris systems resulted in successful assembly of uniform, stable, and non-toxic VP4/VP56/VP35 VLPs. Vaccination with P. pastoris VLPs significantly improved survival rate, IgM levels, tissue viral load, and antioxidant immune-related indicators in grass carp.
Article
Multidisciplinary Sciences
Rui Jiang, Wentao Zhu, Zhiwei Liao, Chunrong Yang, Jianguo Su
Summary: TLR7 plays a crucial role in sensing viral ssRNA and dsRNA, but there are differences in the mechanism of dsRNA sensing between fish and tetrapods.
Article
Virology
Rui Jiang, Jie Zhang, Zhiwei Liao, Wentao Zhu, Hang Su, Yongan Zhang, Jianguo Su
Summary: This study investigated the latent infection of GCRV-II in asymptomatic grass carp with GCRV infection or exposure history. It was found that GCRV-II was detectable only in the brain of grass carp during latent infection, unlike the multi-tissue distribution observed in natural infection. Additionally, GCRV-II distribution in grass carp was notably affected by ambient temperature. This study provides insights into the mechanisms of GCRV-II latent infection and reactivation, contributing to the prevention and control of GCRV pandemics.
Article
Fisheries
Pengxu Wang, Xingchen Huo, Fengxia Zhao, Qian Liu, Qingqing Tian, Chunrong Yang, Jianguo Su
Summary: This study aimed to investigate the potential effectiveness of VD3 in treating Largemouth bass ranavirus (LMBV). The results showed that feeding Largemouth bass with 15000 IU/Kg and 20000 IU/Kg of VD3 significantly suppressed and cleared LMBV pathogens. In addition, VD3 treatment increased the expression levels of key immune-related genes and enhanced antioxidant and immune activities in the serum. Tissue pathology sections also revealed a dose-dependent relationship between VD3 supplementation and tissue damage.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Maolin Lv, Jingjing Zhang, Weicheng Wang, Rui Jiang, Jianguo Su
Summary: This study corrected the number of grass carp TLR20 members, identified a new TLR20 member, and analyzed its evolutionary and structural characteristics. The results showed that TLR20 plays an important role in antiviral and antibacterial defense.
FISH AND SHELLFISH IMMUNOLOGY REPORTS
(2023)
Article
Fisheries
Ratiporn Thongsoi, Siriluk Maskaew, Panumas Puechpon, Benjaporn Noppradit, Napassawan Inaek, Prapaporn Utarabhand, Phanthipha Runsaeng
Summary: In this study, a PPAE gene called FmPPAE1 was cloned and analyzed in banana shrimp. FmPPAE1 plays a vital role in regulating the proPO system, exhibits antibacterial properties, and contributes to the suppression of bacterial proliferation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jishuang Tan, Paizelaiti Sahaer, Huan Li, Wei Han, Hongyan Sun
Summary: Circular RNAs (circRNA) are involved in gene expression and affect cellular processes such as transcription, splicing, and translation by interacting with microRNA and RNA binding proteins. A study identified circDNAJB6 as a circular RNA abundant in chicken macrophages after bacterial infection, and found that circDNAJB6 exacerbates cellular injury and interacts with multiple miRNAs and target genes, potentially impacting autophagy and MAPK signaling pathways.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Rodrigo Guabiraba, Damaris Ribeiro Rodrigues, Paul T. Manna, Melanie Chollot, Vincent Saint-Martin, Sascha Trapp, Marisa Oliveira, Clare E. Bryant, Brian J. Ferguson
Summary: The innate immune response relies on the ability of host cells to detect and respond to microbial nucleic acids. Toll-like receptors (TLRs) play a crucial role in this process by distinguishing self from non-self. This study focused on TLR21, an avian TLR that recognizes bacterial DNA motifs. The findings suggest that avian TLR21 shares similar activation mechanisms to mammalian TLR9, highlighting the conservation of nucleic acid sensing mechanisms across species.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Safieh Zeinali, Kate Sutton, Lonneke Vervelde
Summary: Chickens have a unique immune structure characterized by the absence of draining lymph nodes and the presence of well-developed mucosa-associated lymphoid tissue. The structure and development of chicken lymphoid tissues in the intestine, particularly Peyer's patches, have been poorly studied. However, the use of CSF1R-eGFP reporter transgenic chickens has allowed for the visualization and investigation of the development and distribution of these tissues. This research provides insights that could benefit mucosal vaccination strategies and the development of targeted vaccine delivery systems.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Retraction
Fisheries
G. -g. Li, Z. -z. Guo, X. -f. Ma, N. Cao, S. -n. Geng, Y. -q. Zheng, M. -j. Meng, H. -h. Lin, G. Han, G. -j. Du
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jinyue Yang, Peng Zhou, Wanrong Wu, Qingxiang Zhang, Dong Chen, Rui Luo
Summary: This study cloned and characterized the full-length sequence of duck MRE11 (duMRE11) for the first time, and found that this protein plays an important role in the innate immune response of ducks.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Mengjiao Guo, Jiaqi Zhang, Mingtao Li, Xiaorong Zhang, Yantao Wu
Summary: rNLRC3 negatively regulates the NF -KB signaling pathway induced by P. multocida in rabbits by inhibiting the activation of NF -KB, reducing the expression of inflammatory cytokines, and interacting with rTRAF4 and rTRAF6. The NACHT-LRR domain is the functional domain of rNLRC3. These findings provide insights into the important role of rNLRC3 in combating P. multocida infection.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Yinan Li, Xingye Lian, Wenwen Yang, Jinyuan Leng, Jiejie Sun, Yu Liu, Siqi Fan, Lingling Wang, Linsheng Song
Summary: The DM9-containing protein CgDM9CP-7 plays an important role in the innate immune response of molluscs. It is constitutively expressed in various tissues and is up-regulated in haemocytes after stimulation by Vibrio splendidus and lipopolysaccharide (LPS). CgDM9CP-7 binds to various pathogens and exhibits antimicrobial activity, while also regulating the expression of CgMyD88 and CgIL-17.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Xiuwen Bao, Haoran Song, Liying He, Yong Li, Shuqi Niu, Jinlin Guo
Summary: This study focuses on the interaction between Hepialus xiaojinensis larvae and Ophiocordyceps sinensis during the infection process. The results show that the infected third instar larvae enhance antioxidant defense ability, while the infected fourth instar larvae reduce this ability and allocate more energy to growth and development.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
