Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 38, Issue 1, Pages 98-107Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2012.04.010
Keywords
Grass carp Foxp3; Transcription factor binding sites; mRNA expression; Protein levels; Rapamycin
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Funding
- National Natural Science Foundation of China [30972280, 31101877]
- Science and Technology Committee of Sichuan Province [2011FZ0007]
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In this study, we cloned grass carp foxp3 (gcfoxp3) gene including 5' flanking region and determined its expression profiles in vivo under immunosuppressive conditions. Sequence analysis revealed that the promoter of gcfoxp3 contains AP-1, AML-1/Runx1, NF-kappa b and GATA-3 binding sites, which positively or negatively regulate mammalian foxp3 expression. In addition, the intron 11 of gcfoxp3 contains some putative binding sites including AP-1, NFAT, Smad3, RAR, CREB/ATF and FOX01, which are corresponding to their locations in the proximal intronic enhancers of human foxp3. In an in vivo model of grass carp, an immunosuppressive agent rapamycin was showed to stimulate gcfoxp3 mRNA expression in thymus, gill, head kidney and spleen after bacterial challenge. Moreover, rapamycin increased gcFoxp3 protein levels with an additive manner in the infected fish. These findings support the involvement of fish Foxp3 in immune response and highlight a possible signaling pathway that regulates teleost Foxp3 expression. (C) 2012 Elsevier Ltd. All rights reserved.
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