Article
Physiology
Chongxiao Chen, Dima Lynn White, Brett Marshall, Woo Kyun Kim
Summary: The study indicated that injections of 25OHD and 1,25OHD induced chicken embryo osteogenesis and adipogenesis, but inhibited myogenesis during early development. Higher dosage of 25OHD showed a possibility of longer retention time in the embryos.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Hua Kui, Bo Ran, Maosen Yang, Xin Shi, Yingyu Luo, Yujie Wang, Tao Wang, Diyan Li, Surong Shuai, Mingzhou Li
Summary: Chickens are important sources of protein for human diet and are used in biomedical research. The skeletal muscles of chickens have diverse origins, shapes, metabolic features, and physical functions, making increasing meat production a key goal in the poultry industry. Previous gene expression studies have overlooked the differences among various types of skeletal muscles. In this study, the researchers sequenced 58 samples from 10 different skeletal muscles of White Plymouth Rock chickens and generated a comprehensive transcriptional map, providing valuable information on muscle fiber characteristics.
Article
Multidisciplinary Sciences
Marina Kaiser, Timo H. Luedtke, Lena Deuper, Carsten Rudat, Vincent M. Christoffels, Andreas Kispert, Mark-Oliver Trowe
Summary: This study identifies TBX2 as a key transcription factor for inner hair cells and inner supporting cells, acting as a coding factor in prosensory progenitors to determine the inner compartment of the sensory epithelium and play a crucial role in inducing and maintaining inner hair cell fate.
NATURE COMMUNICATIONS
(2022)
Review
Agriculture, Dairy & Animal Science
Kristina Gvozdanovic, Zlata Kralik, Zarko Radisic, Manuela Kosevic, Gordana Kralik, Ivona Djurkin Kusec
Summary: The addition of bioactive compounds to chicken feed improves the health and production traits of the animals. Nutrigenomics helps understand the molecular relationship between nutrients in the feed and the genes that affect desired phenotypes. This knowledge can be used to produce high-quality chicken products that positively impact human health.
Article
Agriculture, Dairy & Animal Science
R. S. Bisinotto, E. S. Ribeiro, L. F. Greco, D. Taylor-Rodriguez, A. D. Ealy, H. Ayres, F. S. Lima, N. Martinez, W. W. Thatcher, J. E. P. Santos
Summary: This study evaluated the effects of follicular wave and progesterone concentration on growth of the ovulatory follicle, conceptus elongation, uterine IFN-tau concentration, and transcriptome of conceptus and endometrium in pregnant cows. The results indicated that progesterone concentration had an impact on the growth of ovulatory follicle, but overall had minimal effects on the conceptus and endometrium transcriptome.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Yi Wang, Yu-Qing Zhang, Zi-Wei Wu, Ting Fang, Fang Wang, Han Zhao, Zhi-Qiang Du, Cai-Xia Yang
Summary: This study evaluated the expression profiles of six candidate genes as internal reference genes in the ovaries of chicken embryos at different developmental stages. The most stable internal reference genes were identified as Eef1a and Rpl15, while Cvh was the least stable. Furthermore, the expression levels of three marker genes for oocyte meiosis entry were normalized using Eef1a, Rpl15, or their combinations.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Environmental Sciences
Seyed Jamaleddin Shahtaheri, Zahra Goodarzi, Esmaeil Karami, Ali Khavanin, Mahmoud Ghazi Khansari, Mehrafarin Kiani, Ali Rashidy-Pour
Summary: This study investigated the impact of acute exposure to white noise and Al2O3 NPs, alone and in combination, on the hearing and structural functions of the cochlea in rats. The results showed that both independent and combined exposure to white noise and Al2O3 NPs damaged the hearing and structural functions of the cochlea, leading to an increase in temporary threshold shift (TTS) and permanent threshold shift (PTS).
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
Mohamed Ashfaque, A. Nafeez Ahmed, S. Mohammed Safiullah, G. Taju, S. Abdul Majeed, A. S. Sahul Hameed, K. Anver Basha
Summary: Carbon nanotubes (CNTs) have been widely used in developing polymer hybrid coatings for anticorrosive application. In this study, a novel poly [(3,5-dimethyl-lH-pyrazole-1-yl) methyl methacrylate-co-glycidyl methacrylate] (PyM) was prepared and single-wall carbon nanotubes (SWCNT) were incorporated in PyM to form nanocomposites. The toxicity of the nanocomposites was evaluated in vivo and in vitro using zebrafish embryos and gill cell line of zebrafish (DrG). The results showed that nanocomposites with a concentration of 400 μg/ml of PyM in 1.0% of SWCNT were non-toxic and recommended for anticorrosive application. Nanocomposites with higher SWCNT content showed toxic effects. The nanocomposites with 1.5% of SWCNT delayed hatching rate, decreased survival rate and heart beat in zebrafish embryos, and inducedmorphological changes in DrG cells. Gene expression studies revealed that nanocomposites with high SWCNT concentration induced oxidative stress. The immersion study of the recommended concentration of PyM-SWCNT on mild steel (MS) in sea water showed effective corrosion protection without leaching behavior. The findings suggest that the nanocomposites with the novel polymer have potential for industrial anticorrosive applications.
Article
Environmental Sciences
Xueying Ma, Wenhua Li
Summary: This study evaluated the potential effects of the fungicide Amisulbrom (AML) on fish using a zebrafish model. Results showed that AML-treated zebrafish embryos exhibited severe developmental defects, including pericardial edema and decreased heart rates. Key marker genes associated with cardiovascular development were abnormally expressed in response to AML treatment, suggesting a specific impact on cardiovascular development by AML.
Article
Genetics & Heredity
Victor Gourain, Isabelle Duluc, Claire Domon-Dell, Jean-Noel Freund
Summary: The study focuses on whether a gene involved in distinct tissue or cell functions exerts a core of common molecular activities, specifically looking at the transcription factor and regulator of chromatin accessibility encoded by the Cdx2 homeobox gene. By integrating RNAseq data in mouse embryogenesis and analyzing ChIPseq data, a core set of common genes responsive to CDX2 during developmental processes were identified. The relevance of these findings was highlighted by demonstrating altered expression of human orthologous genes to the CDX2-responsive genes in human malignancies.
FRONTIERS IN GENETICS
(2021)
Article
Reproductive Biology
Tamie Guibu de Almeida, Rodolfo Daniel Mingoti, Leticia Signori de Castro, Adriano Felipe Perez Siqueira, Thais Rose dos Santos Hamilton, Patricia Kubo Fontes, Marcelo Fabio Gouveia Nogueira, Mayra Fernanda Alves, Andrea Cristina Basso, Marcella Pecora Milazzotto, Mayra Elena Ortiz D'Avila Assumpcao
Summary: The study evaluated the impact of different sires on the outcome of in vitro embryo production, finding that low in vitro fertility bulls have higher rates of polyspermy and produce embryos with higher levels of transcripts related to apoptosis and cell damage pathways.
Review
Immunology
Paul Garcia, Yan Wang, Jean Viallet, Zuzana Macek Jilkova
Summary: The chicken embryo model has emerged as an alternative to rodents in immune-based studies, offering advantages such as cost-effectiveness, time efficiency, and ease of use. It can be utilized for specific experimental hypotheses or preclinical testing, potentially serving as a valuable tool in biomedical research.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Veterinary Sciences
Cristina Cuello, Cristina A. Martinez, Josep M. Cambra, Alejandro Gonzalez-Plaza, Inmaculada Parrilla, Heriberto Rodriguez-Martinez, Maria A. Gil, Emilio A. Martinez
Summary: The study showed that vitrification altered the gene expression pattern of porcine morulae produced in vivo, leading to disruptions in metabolic pathways that may interfere with embryo quality and developmental potential.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Jose Felipe Warmling Spricigo, Ana Luiza Silva Guimaraes, Andrielle Thainar Mendes Cunha, Ligiane de Oliveira Leme, Marcos Coura Carneiro, Mauricio Machaim Franco, Margot Alves Nunes Dode
Summary: This study aimed to evaluate the potential to predict the quality of oocytes by measuring the transcript abundance of a panel of candidate genes in immature and/or mature cumulus cells. The results showed that none of the selected genes can accurately predict the potential of oocytes to develop into embryos. Additionally, cumulus cell biopsy negatively affects embryo development, blastocyst cell number, and apoptotic cell ratio.
Article
Agriculture, Dairy & Animal Science
Jianfei Chen, Ziwei Chen, Jilong Zhang, Wuchao Zhuang, Xiaotong Zheng
Summary: This study utilized different algorithms to evaluate the stability of internal reference genes in the gastrointestinal tract of chickens. The most suitable reference genes for each type of GI tissue were identified. Commonly used internal reference genes exhibited poor stability and are not recommended for future gene expression studies in the GI region.
Article
Cell Biology
Ciqing Yang, Xiaoying Li, Lihong Guan, Shuanqing Li, Liang Qiao, Juntang Lin
HISTOCHEMISTRY AND CELL BIOLOGY
(2019)
Article
Cell Biology
Jinjin Yu, Lujun Shen, Yanli Liu, Hong Ming, Xinxing Zhu, Maoping Chu, Juntang Lin
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2020)
Review
Reproductive Biology
Yanli Liu, Zhiqin Zhang, Fen Yang, Hongmei Wang, Shengying Liang, Huiling Wang, Jun Yang, Juntang Lin
BIOLOGY OF REPRODUCTION
(2020)
Review
Gastroenterology & Hepatology
Jiang Du, Yu Ji, Liang Qiao, Yanli Liu, Juntang Lin
LIVER INTERNATIONAL
(2020)
Article
Neurosciences
Lu Wang, Dandan Zhao, Mengmeng Wang, Yuan Wang, Martin Vreugdenhil, Juntang Lin, Chengbiao Lu
FRONTIERS IN CELLULAR NEUROSCIENCE
(2020)
Review
Cell & Tissue Engineering
Jiang Du, Han Li, Jie Lian, Xinxing Zhu, Liang Qiao, Juntang Lin
STEM CELL RESEARCH & THERAPY
(2020)
Article
Cell & Tissue Engineering
Yanli Liu, Shengying Liang, Fen Yang, Yuliang Sun, Lidan Niu, Yakun Ren, Hongmei Wang, Yanan He, Jiang Du, Jun Yang, Juntang Lin
STEM CELL RESEARCH & THERAPY
(2020)
Review
Biochemistry & Molecular Biology
Xiaoying Li, Yunxiao Li, Shuanqing Li, Han Li, Ciqing Yang, Juntang Lin
Summary: The Sonic hedgehog (Shh) signaling pathway plays crucial roles in the development of the central nervous system (CNS) and related diseases. Abnormal signaling can lead to the occurrence of neurological disorders. Studies have classified the Shh signaling pathway into canonical and non-canonical pathways.
CELL BIOCHEMISTRY AND FUNCTION
(2021)
Article
Cell & Tissue Engineering
Yonghai Li, Junzheng Yang, Meng Li, Xiaoyue Zhang, Jiang Du, Xinghua Zhao, Zhihao Xu, Juntang Lin
CELLULAR REPROGRAMMING
(2020)
Article
Obstetrics & Gynecology
Shenghui Zhang, Yuliang Sun, Dongli Jiang, Tongtong Chen, Ruihong Liu, Xinyi Li, Yilin Lu, Liang Qiao, Ying Pan, Yanli Liu, Juntang Lin
Summary: This study aimed to establish a stable animal model of intrauterine adhesion (IUA) using a minimally invasive method that recapitulates the clinicopathologic characteristics of IUA. The results showed a positive correlation between ethanol treatment time and severity of uterine damage and kidney dysfunction, particularly in the EtOH-40 s group. Overall, the study successfully established a mouse model of endometrial injury using a minimally invasive transcervical ethanol perfusion technique, providing a basis for further research on IUA pathogenesis and therapies.
REPRODUCTIVE SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Rui Guo, Yun Yang, Di Zhang, Jiang Du, Xinxing Zhu, Yanli Liu, Fen Yang, Juntang Lin
Summary: Multiple signaling pathways are involved in tumor development, making bispecific antibodies more suitable for treating complex tumor diseases. A novel PE-based bispecific immunotoxin designed in this study showed improved efficacy against HER2-positive tumors. Transcriptome sequencing and real-time PCR were used to verify the results, indicating the potential of these bispecific immunotoxins for anti-HER2 cancer therapeutics.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Biotechnology & Applied Microbiology
Jie Lian, Xinxing Zhu, Jiang Du, Beijia Huang, Fengting Zhao, Chunya Ma, Rui Guo, Yangxia Zhang, Longkai Ji, Badrul Hisham Yahaya, Juntang Lin
Summary: Menstrual blood-derived stem cells can alleviate acute lung inflammation and injury through their extracellular vesicle-transmitted miR-671-5p. This miRNA directly targets AAK1 and regulates the activation of NF-kB signaling. This study provides insights into the molecular basis of how extracellular vesicles from mesenchymal stem cells improve pulmonary inflammatory injury and highlights the functional importance of the miR-671-5p/AAK1 axis.
Article
Biochemistry & Molecular Biology
Xinxing Zhu, Beijia Huang, Fengting Zhao, Jie Lian, Lixiang He, Yangxia Zhang, Longkai Ji, Jinghang Zhang, Xin Yan, Taoling Zeng, Chunya Ma, Yinming Liang, Chen Zhang, Juntang Lin
Summary: NF-kappa B activation leads to MRSA-induced lung inflammation, and FOXN3 can inhibit NF-kappa B signaling to reduce the inflammatory response. FOXN3 competes with I kappa B alpha for binding to hnRNPU, preventing I kappa B alpha degradation and NF-kappa B activation. Phosphorylation of FOXN3 by p38 induces dissociation from hnRNPU, leading to NF-kappa B activation. FOXN3 phosphorylation is clinically correlated with pulmonary inflammatory disorders.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Yanli Liu, Rongcheng Niu, Wenzhong Li, Juntang Lin, Christof Stamm, Gustav Steinhoff, Nan Ma
CELLULAR AND MOLECULAR LIFE SCIENCES
(2019)
Article
Cell Biology
Qing Liu, Jiang Du, Jinyu Fan, Wenqiang Li, Weiyun Guo, Huigen Feng, Juntang Lin
