Journal
DEVELOPMENT GROWTH & DIFFERENTIATION
Volume 51, Issue 9, Pages 769-775Publisher
WILEY
DOI: 10.1111/j.1440-169X.2009.01135.x
Keywords
Arabidopsis; gene expression; heat shock; medaka; zebrafish
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [KH: 15300117, SO: 17659062]
- Japan Space Forum
- Japan Society for the Promotion of Science [19GS0315]
- University of Hyogo
- Hyogo Science and Technology Association
- Grants-in-Aid for Scientific Research [19101002] Funding Source: KAKEN
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Heat shock promoters are powerful tools for the precise control of exogenous gene induction in living organisms. In addition to the temporal control of gene expression, the analysis of gene function can also require spatial restriction. Recently, we reported a new method for in vivo, single-cell gene induction using an infrared laser-evoked gene operator (IR-LEGO) system in living nematodes (Caenorhabditis elegans). It was demonstrated that infrared (IR) irradiation could induce gene expression in single cells without incurring cellular damage. Here, we report the application of IR-LEGO to the small fish, medaka (Japanese killifish; Oryzias latipes) and zebrafish (Danio rerio), and a higher plant (Arabidopsis thaliana). Using easily observable reporter genes, we successfully induced gene expression in various tissues in these living organisms. IR-LEGO has the potential to be a useful tool in extensive research fields for cell/tissue marking or targeted gene expression in local tissues of small fish and plants.
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