Article
Multidisciplinary Sciences
Aileen Julia Riesle, Meijiang Gao, Marcus Rosenblatt, Jacques Hermes, Helge Hass, Anna Gebhard, Marina Veil, Bjorn Gruening, Jens Timmer, Daria Onichtchouk
Summary: This study investigates the combinatorial mechanisms of genome activation and shows that zebrafish genome activators Pou5f3 and Nanog exhibit different roles in the activation process. They act as pioneer-like transcription factors on synergistic enhancers, promoting histone acetylation and transcription activation. However, on antagonistic enhancers, one of the factors acts as a non-pioneer-like transcription factor, competing with the activator and blocking its effects. This activator-blocker mechanism restricts widespread transcriptional activation and prevents premature expression of late developmental genes in early embryos.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Hyunjoon Kim, Soohyun Jang
Summary: This study reveals the functions of Mettl3 during spatial neural patterning in Xenopus, including its roles in anteroposterior neural patterning, neural crest specification, and neuronal cell differentiation. Knockdown of Mettl3 leads to anteriorization of neurulae and tailbud embryos, loss of neural crest and neuronal cells, and inhibition of canonical WNT signaling.
MOLECULAR AND CELLULAR BIOLOGY
(2021)
Review
Cell & Tissue Engineering
Rui-Ting Zhou, Yi-Ran Ni, Fan-Jun Zeng
Summary: This review summarizes the research progress on long noncoding RNAs involved in the regulation of OCT4 expression, which is maintained through various mechanisms such as methylation, chromosome spatial folding, and competitive binding with microRNAs. These findings provide important insights into the maintenance of pluripotency in stem cells.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Multidisciplinary Sciences
Mikihito Shibata, Kartik Pattabiraman, Belen Lorente-Galdos, David Andrijevic, Suel-Kee Kim, Navjot Kaur, Sydney K. Muchnik, Xiaojun Xing, Gabriel Santpere, Andre M. M. Sousa, Nenad Sestan
Summary: The study highlights the crucial role of retinoic acid signaling in the development of the prefrontal cortex (PFC) and suggests its potential impact on the evolutionary expansion of the PFC. This research provides insights into the molecular mechanisms underlying cognitive flexibility and working memory, as well as disorders such as autism and schizophrenia.
Article
Biology
Takayoshi Yamamoto, Yuta Kambayashi, Yuta Otsuka, Boni Afouda, Claudiu Giuraniuc, Tatsuo Michiue, Stefan Hoppler, Makoto Sato
Summary: This study revealed the importance of a receptor feedback mechanism in shaping a steep gradient of Wnt signaling and demonstrated the local inhibition of Wnt signaling through restriction of sFRP1 spreading, ensuring reproducible patterning of the thin pericardium.
Article
Biochemistry & Molecular Biology
Kimiko Takebayashi-Suzuki, Misa Uchida, Atsushi Suzuki
Summary: Zbtb21 plays a crucial role in AP axis formation and neural development in early Xenopus embryogenesis, working in collaboration with Zbtb14.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Kimiko Takebayashi-Suzuki, Misa Uchida, Atsushi Suzuki
Summary: Xenopus Zbtb21 plays an essential role in AP axis formation during early embryonic development, working in coordination with Zbtb14 to pattern the dorsal-ventral and anterior-posterior axes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Developmental Biology
Hui Wang, Chengdong Wang, Qi Long, Yuan Zhang, Meiling Wang, Jie Liu, Xufeng Qi, Dongqing Cai, Gang Lu, Jianmin Sun, Yong-Gang Yao, Wood Yee Chan, Wai Yee Chan, Yi Deng, Hui Zhao
Summary: Kindlin2 is essential for neural crest formation in Xenopus embryos independently of integrins, and it regulates the FGF pathway to promote the stability of FGF receptor 1.
Article
Developmental Biology
Takayoshi Yamamoto, Toki Kaneshima, Kohei Tsukano, Tatsuo Michiue
Summary: The nervous system is derived from three neuroectodermal regions and their differentiation is regulated by morphogens. The role of the extracellular component heparan sulfate (HS) in regulating morphogen gradients in neural patterning is still debated. This study focused on Hs6st1, an HS modification enzyme, and found that it selectively regulates morphogen distribution in neuroectodermal patterning. The results suggest that cell-autonomous 6-O sulfation of HS affects neuroectodermal patterning through both morphogen accumulation and dispersal.
DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Yang Yang, Xiaohong Wang, Yiqiang Liu, Ying Hu, Zhongwu Li, Ziyu Li, Zhaode Bu, Xiaojiang Wu, Lianhai Zhang, Jiafu Ji
Summary: SALL4 is overexpressed in a proportion of gastric cancer patients and is associated with unfavorable clinicopathological features. It plays a significant role in the Wnt/beta-catenin signaling pathway in cancer progression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Takashi Fukaya
Summary: Expression of gap and pair-rule genes is crucial for body segmentation in Drosophila embryogenesis, and the length of the transcription period plays a major role in determining spatial patterning of gene expression in early embryos. Enhancer interference can influence transcriptional activation and impact the refinement of gene expression patterns during animal development.
Article
Genetics & Heredity
Lei Luo, Yan Shi, Huanan Wang, Zizengchen Wang, Yanna Dang, Shuang Li, Shaohua Wang, Kun Zhang
Summary: The emergence of the first three lineages during development is regulated by a network of transcription factors, but the specific regulation varies among different mammals. This study establishes a gene inactivation system in bovine early embryos and investigates the functional consequences of three critical lineage-specific genes (SOX2, OCT4, and CDX2). The results reveal that SOX2 knockout leads to a failure in pluripotency establishment in bovine blastocysts, affecting the expression of other lineage-specific genes.
Review
Medical Laboratory Technology
Fatma A. Abouelnazar, Xiaoxin Zhang, Maoye Wang, Jiahui Zhang, Dan Yu, Xueyan Zang, Jiayin Zhang, Yixin Li, Jing Xu, Qiurong Yang, Yue Zhou, Haozhou Tang, Yanzheng Wang, Jianmei Gu, Xu Zhang
Summary: SALL4 is a transcription factor that regulates the self-renewal and pluripotency of embryonic stem cells. Its aberrant expression in cancer is associated with tumor progression and therapeutic response. SALL4 plays a role in controlling key signaling pathways and downstream gene expression, making it a promising biomarker and therapeutic target for cancer.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2023)
Article
Cell Biology
Tali Abbou, Liat Bendelac-Kapon, Audeliah Sebag, Abraham Fainsod
Summary: A study found that loss of RA metabolic network genes in the X. laevis genome leads to tighter and more efficient regulation, enabling it to respond to complex regulatory conditions.
Article
Multidisciplinary Sciences
Johanna Diener, Arianna Baggiolini, Mattias Pernebrink, Damian Dalcher, Luigi Lerra, Phil F. Cheng, Sandra Varum, Jessica Hausel, Salome Stierli, Mathias Treier, Lorenz Studer, Konrad Basler, Mitchell P. Levesque, Reinhard Dummer, Raffaella Santoro, Claudio Cantu, Lukas Sommer
Summary: Melanoma cells rely on developmental programs during tumor initiation and progression. SALL4 is a negative regulator of melanoma phenotype switching where its loss leads to the acquisition of an invasive phenotype. This is mediated through interaction with HDAC2 and co-binding to a set of invasiveness genes.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Aysu Uygur, John Young, Tyler R. Huycke, Mervenaz Koska, James Briscoe, Clifford J. Tabin
DEVELOPMENTAL CELL
(2016)
Article
Oncology
Yan Ding, Elissa A. Boguslawski, Bree D. Berghuis, John J. Young, Zhongfa Zhang, Kim Hardy, Kyle Furge, Eric Kort, Arthur E. Frankel, Rick V. Hay, James H. Resau, Nicholas S. Duesbery
MOLECULAR CANCER THERAPEUTICS
(2008)
Article
Multidisciplinary Sciences
John J. Young, Jennifer M. Cherone, Yannick Doyon, Irina Ankoudinova, Farhoud M. Faraji, Andrew H. Lee, Catherine Ngo, Dmitry Y. Guschin, David E. Paschon, Jeffrey C. Miller, Lei Zhang, Edward J. Rebar, Philip D. Gregory, Fyodor D. Urnov, Richard M. Harland, Bryan Zeitler
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2011)
Editorial Material
Biochemistry & Molecular Biology
John J. Young, Phil Grayson, Clifford J. Tabin
Article
Biochemistry & Molecular Biology
John J. Young, Phil Grayson, Scott Edwards, Clifford J. Tabin
Article
Cell Biology
Maggie Clancy, Isabel S. Wade, John J. Young
Summary: Plastic pollution has negative impacts on ecosystems and human health globally, and single-use plastic is the main component of marine litter in some areas. In life science laboratories, single-use plastic products are used to ensure experimental reliability, contributing to the overall plastic waste problem. This study explores the potential of reusing plastic culture dishes and proposes methods to mitigate single-use plastic waste in developmental biology research laboratories. The findings demonstrate that cleaning and reusing laboratory plastic does not adversely affect experimental outcomes and can be an environmentally conscious approach for sterilizing plastic before reuse or recycling. Additionally, the study highlights the potential economic benefits and waste reduction associated with recycling single-use plastics in research laboratories.
Editorial Material
Developmental Biology
John J. Young
Review
Developmental Biology
John J. Young, Clifford J. Tabin
DEVELOPMENTAL BIOLOGY
(2017)
Article
Developmental Biology
John J. Young, Rachel A. S. Kjolby, Gloria Wu, Daniel Wong, Shu-wei Hsu, Richard M. Harland
DEVELOPMENTAL BIOLOGY
(2017)
Article
Oncology
Philippe Depeille, John J. Young, Elissa A. Boguslawski, Bree D. Berghuis, Eric J. Kort, James H. Resau, Arthur E. Frankel, Nicholas S. Duesbery
CLINICAL CANCER RESEARCH
(2007)
Article
Microbiology
John J. Young, Jennifer L. Bromberg-White, Cassandra Zylstra, Joseph T. Church, Elissa Boguslawski, James H. Resau, Bart O. Williams, Nicholas S. Duesbery
Article
Marine & Freshwater Biology
Ahna L. Van Gaest, Craig M. Young, John J. Young, Alicia R. Helms, Shawn M. Arellano
MARINE ECOLOGY-AN EVOLUTIONARY PERSPECTIVE
(2007)
Article
Biochemistry & Molecular Biology
XD Liang, JJ Young, SA Boone, DS Waugh, NS Duesbery
JOURNAL OF BIOLOGICAL CHEMISTRY
(2004)