Journal
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
Volume 26, Issue 11, Pages 1124-1135Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jnutbio.2015.05.006
Keywords
Adipogenesis; PPAR gamma; MicroRNAs; Structural-dependent; Membrane disturbing effects
Funding
- Chinese Ministry Program for New Century Excellent Talents in University [NCET-12-0865]
- National Natural Science Foundation of China [31271833]
- Special Fund for Agro-scientific Research in the Public Interest [201203047]
- Fundamental Research Funds for the Central Universities [2013PY022]
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The effects of four proanthocyanidin dimers including epicatechin-(4 beta -> 8, 2 beta -> O -> 7)-epicatechin (A-type EC dimer), epicatechin-(4 beta -> 8)-epicatechin (B-type EC dimer), epicatechin-3-gallate-(4 beta -> 8, 2 beta -> O -> 7)-epicatechin-3-gallate (A-type ECG dimer) and epigallocatechin-3-gallate-(4 beta -> 8, 2 beta -> O -> 7)-epigallocatechin-3-gallate (A-type EGCG dimer) on 3T3-L1 preadipocyte cell differentiation and the underlying mechanisms were explored and compared. The results showed that A-type ECG dimer and A-type EGCG dimer significantly reduced the intracellular lipid accumulation in 3T3-L1 preadipocyte cells by targeting miR-27a and miR-27b as well as peroxisome proliferator-activated receptor la (PPAR gamma) in the early stage of differentiation, while A-type EC dimer and B-type EC dimer showed little effect. In addition, our results revealed that the inhibitory effects of proanthocyanidin dimers on 3T3-L1 preadipocyte differentiation were highly structure-dependent and the effects were associated with the dimer-membrane interactions. The presence of galloyl moieties and A-type linkage within the structure of proanthocyanidins might be crucial for their inhibitory effect on adipogenesis. The strong disturbing effects of A-type ECG and A type EGCG dimers on the fluidity, hydrophobicity and permeability of membrane of 3T3-L1 preadipocyte cell were at least, in part, responsible for their distinct inhibitory effects on adipocyte hyperplasia. (C) 2015 Elsevier Inc. All rights reserved.
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