Article
Chemistry, Physical
Tianqi Guo, Necla Asli Kocak Oztug, Pingping Han, Saso Ivanovski, Karan Gulati
Summary: Bioactivity of implants is influenced by the chemistry and topography of the surface, with nano-engineered implants often having concurrent modifications in both aspects. This study focused on how topography and chemistry of nanostructures affect protein adhesion and cell metabolism/proliferation, highlighting the significant impact of topography on cell alignment and bioactivity. The research aims to optimize parameters for the next generation of highly bioactive implants through a better understanding of the influence of dual topographically/chemically modified nano-engineered surfaces.
APPLIED SURFACE SCIENCE
(2021)
Article
Chemistry, Physical
Karan Gulati, Mateusz Czerwinski, Marta Michalska-Domanska, Ruben Del Olmo Martinez
Summary: This review discusses and details the influence of electrolyte aging in Ti anodization on the fabrication of controlled nanostructures, as well as its impact on the morphology, chemistry, stability, and application-specific performance of nanostructures.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2022)
Article
Materials Science, Multidisciplinary
Tianqi Guo, Saso Ivanovski, Karan Gulati
Summary: The study evaluates nanostructure self-ordering on clinically relevant curved/micro-rough Ti, contributing to an improved understanding of implant nanoengineering.
MATERIALS & DESIGN
(2022)
Article
Materials Science, Multidisciplinary
A. Jayasree, N. T. Raveendran, T. Guo, S. Ivanovski, K. Gulati
Summary: This study proposed the fabrication of dual micro-nano anisotropic titania nanopores on micro-machined titanium to enhance osteoblast performance, which was evaluated through culture of primary human osteoblasts and an oral salivary biofilm model. The results showed that the dual micro-nanotopography significantly promoted osteoblast proliferation and differentiation without influencing oral biofilm formation.
MATERIALS TODAY ADVANCES
(2022)
Article
Chemistry, Physical
Josef Hlinka, Kamila Dostalova, Kristina Cabanova, Roman Madeja, Karel Frydrysek, Jan Koutecky, Zuzana Rybkova, Katerina Malachova, Osamu Umezawa
Summary: Anodization coloring is commonly used for differentiation of titanium tools or implants. Commercial purity titanium grade 4 plates were tested for their properties. The coloring process was done using different potentials in citric acid solution. The anodization process significantly decreases corrosion potential and higher potential results in higher polarization resistance but lower corrosion potential. The anodization process at 75 V produces surfaces with the lowest corrosion rate and noblest corrosion potential, without affecting titanium cytotoxicity.
Article
Biophysics
Ying Yang, Sanjun Fan, James A. Webb, Yuanqing Ma, Jesse Goyette, Xueqian Chen, Katharina Gaus, Richard D. Tilley, Justin Gooding
Summary: This study presents an electrochemical approach to reversible fluorescence switching of enhanced green fluorescent proteins (EGFP) on indium tin oxide coated glass. The method allows efficient switching between bright (ON) and dim (OFF) states at the single molecule level. The electrochemical fluorescence switching is fast, reversible, and can be incorporated into advanced fluorescence microscopy.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Materials Science, Coatings & Films
Ika Maria Ulfah, Diah Ayu Fitriani, Siti Amalina Azahra, Aghni Ulma Saudi, Muhammad Kozin, Razie Hanafi, Prabowo Puranto, Bambang Sugeng, Yudi Nugraha Thaha, Aga Ridhova, Muhammad Prisla Kamil
Summary: Titanium dioxide nanotubes (TNTs) grown on the surface of titanium implants play a significant role in preventing tissue infection and implant failure. This study investigates the role of different counter electrodes in the formation of TNTs with varying shapes and surface properties. The use of platinum and graphite cathodes results in circular TNTs, while stainless steel cathode leads to hexagonal-shaped tubes with particle debris on the surface. The surface microstructure of TNTs affects their roughness, hydrophilicity, and ability to facilitate the growth of bone-forming apatite during osseointegration.
SURFACE & COATINGS TECHNOLOGY
(2023)
Article
Materials Science, Ceramics
Alberto Z. Fatichi, Mariana G. de Mello, Karina D. Pereira, Luisa G. M. Antonio, Augusto D. Luchessi, Rubens Caram, Alessandra Cremasco
Summary: The electrochemical, structural, and biological properties of self-organized amorphous and anatase/rutile titanium dioxide (TiO2) nanotubes deposited on Ti-35Nb-4Zr alloy through anodization-induced surface modification were investigated. The study found that anatase TiO2 exhibited higher surface corrosion resistance and cell viability compared to amorphous TiO2, indicating the importance of TiO2 nanotube crystallization in the material's electrochemical behavior and biocompatibility.
CERAMICS INTERNATIONAL
(2022)
Article
Materials Science, Coatings & Films
Tianqi Guo, Saso Ivanovski, Karan Gulati
Summary: This study investigates the influence of electrolyte aging on the fabrication and properties of TiO2 nanotubes/nanopores. The results identify the optimal electrolyte aging conditions for producing well-ordered nanotubes/nanopores, which can enhance the performance of biomedical implants.
SURFACE & COATINGS TECHNOLOGY
(2022)
Review
Engineering, Biomedical
Divya Chopra, Karan Gulati, Saso Ivanovski
Summary: This review discusses the effectiveness of antibacterial nanotubes in combating bacterial infections and the avoidance of cytotoxicity issues. By controlling nanotopographies and locally eluting antibiotics, tailored antibacterial efficacy of titanium implants can be achieved.
ACTA BIOMATERIALIA
(2021)
Article
Nanoscience & Nanotechnology
Anna Paulla Simon, Andressa Rodrigues, Janaina Soares Santos, Francisco Trivinho-Strixino, Bruno Leandro Pereira, Carlos Mauricio Lepienski, Henrique Emilio Zorel Junior, Mariana de Souza Sikora
Summary: By altering synthesis variables, the morphology and structural properties of TiO2 nanotube arrays can be tailored for specific applications. This study investigated the effects of electrolyte-containing ions and annealing temperature on the structural, morphological, and mechanical parameters of TiO2 films for potential biomedical applications. The results showed that different synthesis conditions led to variations in the morphology and structure of TiO2 nanotube arrays. Annealing temperature affected the stability of nanostructures, but had limited effects on hardness and Young's modulus. The ability to tailor specific properties through synthesis conditions could enable personalized treatments and improved in vivo performance.
Article
Chemistry, Physical
Ayushi Tanwar, Ahana Mukherjee, Debasish Nath, Ishita Das, Ranjita Ghosh Moulick, Jaydeep Bhattacharya
Summary: Electron transport and transition within a molecule play important roles in cellular and biochemical processes. In this study, we immobilized Strep-TMR, BSA, and GFP on a solid surface and examined their functionality under a fluorescence microscope. We found that electron transfer in GFP affects the impedance of the system, and this relationship was further explored through photobleaching experiments.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Zhengchuan Zhang, Ruogu Xu, Yang Yang, Chaoan Liang, Xiaolin Yu, Yun Liu, Tianlu Wang, Yi Yu, Feilong Deng
Summary: The study shows that micro/nano-textured hierarchical titanium topography enhances BMSCs adhesion and proliferation in vitro, leading to improved osseointegration in vivo. Different titanium topographies promote exosome secretion from BMSCs through upregulating specific gene expressions, providing insights into enhanced osseointegration through exosome biogenesis and secretion.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Zhaoxia Dai, Zi Ming Wang, Dajiang Zheng, Guang-Ling Song, Danqing Feng, Matthew Dargusch, Hui Chang, Lian Zhou
Summary: The electrochemical behavior of titanium in simulated seawater was significantly impacted by polarization at different anodic potentials, with high potential polarization improving long-term durability and slightly better antifouling performance compared to low potentials.
JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE
(2022)
Article
Engineering, Electrical & Electronic
Wei Tang, Yu Liu, Juan Cheng, Chang Li, Xun Chen
Summary: The proposed method in this paper aims to address the fusion of functional and structural information in cell and molecular biology images through a detail preserving cross network. By utilizing a structural-guided functional feature extraction branch, a functional-guided structural feature extraction branch, and a detail preserving module with multi-scale convolutional blocks, the proposed method outperforms state-of-the-art methods in both qualitative and quantitative evaluations, demonstrating good generalizability in medical imaging fusion applications.
IEEE TRANSACTIONS ON COMPUTATIONAL IMAGING
(2021)
Article
Agronomy
Guili Yang, Siping Chen, Likai Chen, Weiwei Gao, Yuting Huang, Cuihong Huang, Danhua Zhou, Jiafeng Wang, Yongzhu Liu, Ming Huang, Wuming Xiao, Hui Wang, Tao Guo, Zhiqiang Chen
Article
Economics
Likai Chen, Weining Wang, Wei Biao Wu
Summary: This article introduces a semiparametric model for change-point analysis of high-dimensional time series, incorporating dynamic structural break factors. Theoretical properties of estimates for loading functions and factors are studied, along with consistency and asymptotic normality for breakpoint inference. Results hold for both large and small breaks in factor dependency structure, and estimation precision is further illustrated through simulations. Two empirical applications are presented in modeling the dynamics of minimum wage policy in China and analyzing a limit order book dataset.
JOURNAL OF BUSINESS & ECONOMIC STATISTICS
(2021)
Article
Agricultural Engineering
Xiuzhen Chen, Junren Li, Yanting Liu, Daidi Wu, Huiling Huang, Ruoting Zhan, Weiwen Chen, Likai Chen
INDUSTRIAL CROPS AND PRODUCTS
(2020)
Article
Agricultural Engineering
Junren Li, Xiuzhen Chen, Xuanxuan Zhou, Huiling Huang, Daidi Wu, Jiaqi Shao, Ruoting Zhan, Likai Chen
Summary: Trihelix transcription factors play key roles in plant growth, development, and secondary metabolism. In this study, members of the trihelix family were investigated in the medicinal plant Pogostemon cablin, revealing PatGT-1 as a negative regulator in patchoulol synthesis. These findings enhance understanding of the trihelix family in patchouli and provide insights for future research.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Statistics & Probability
Likai Chen, Weining Wang, Wei Biao Wu
Summary: This paper introduces a method for detecting multiple change-points in high-dimensional time series, supported by theoretical consistency and asymptotic distribution. The proposed two-step procedure can capture both the biggest break across different coordinates and aggregating simultaneous breaks over multiple coordinates.
JOURNAL OF THE AMERICAN STATISTICAL ASSOCIATION
(2022)
Article
Economics
Likai Chen, Ekaterina Smetanina, Wei Biao Wu
Summary: This paper introduces a multiplicative nonstationary nonparametric regression model for uncovering the conditional mean function, with applications for estimating pricing equations and risk premium shapes. The model can be seen as a dimension-reduction technique and is particularly useful for small sample sizes.
ECONOMETRICS JOURNAL
(2022)
Article
Agriculture, Multidisciplinary
Xiuzhen Chen, Xiaobing Wang, Daidi Wu, Junren Li, Huiling Huang, Xilin Wang, Ruoting Zhan, Likai Chen
Summary: In the synthesis of patchoulol, the promoter of the patchoulol synthase gene (PatPTSpro) is induced by methyl jasmonate (MeJA). The nucleus-localized AP2/ERF transcription factor PatDREB acts as a transcription activator and binds to PatPTSpro, regulating patchoulol biosynthesis by modulating gene expression. PatDREB also interacts with jasmonate ZIM-domain 4 (JAZ4) and the MYB-related transcription factor PatSWC4, synergistically facilitating patchoulol biosynthesis. However, the transcriptional activation activity of the PatDREB-PatSWC4 complex can be inhibited by PatJAZ4, which can be reversed by JA.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Engineering, Biomedical
Yumei Que, Zhaowenbin Zhang, Yanxin Zhang, Xin Li, Likai Chen, Peier Chen, Caiwen Ou, Chen Yang, Jiang Chang
Summary: Silicate ions, as soluble form of bioactive ceramics, effectively alleviate aortic aneurysm and dissection by regulating the local vascular microenvironment and reducing senescence, inflammation, and cell apoptosis. In vitro experiments show that silicate ions can alleviate senescence, inflammation, and apoptosis of vascular-related cells and regulate their interactions. In vivo studies confirm that silicate ions can effectively alleviate aortic senescence, inflammation, and cell apoptosis, leading to reduced aortic dilation and elastin degradation. This bioactive ceramic derived therapy has the potential to attenuate AAD progression.
BIOACTIVE MATERIALS
(2023)
Article
Agricultural Engineering
Huiling Huang, Daidi Wu, Taoyu Guo, Danhua Zhang, Xilin Wang, Jiexuan Zhuang, Xuan Zou, Lizhen Gong, Ruoting Zhan, Likai Chen
Summary: This study aims to elucidate the regulatory role of the bZIP transcription factor PcbZIP44 in patchoulol biosynthesis. It is found that PcbZIP44 negatively regulates patchoulol biosynthesis by inhibiting the PcPTS gene. These findings provide a better understanding of the transcriptional modulation of patchoulol biosynthesis and highlight the potential regulatory mechanisms involving bZIP TFs.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Horticulture
Shibin Chen, Xiaobing Wang, Hai Zheng, Likai Chen
Summary: In this study, a VIGS system was established for functional characterization of candidate genes in Pogostemon cablin. Silencing of PDS and PTS genes successfully suppressed the biosynthesis of patchouli alcohol and other compounds.
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Lin Zhang, Likai Chen, Xiaoqian (Annie) Yu, Claire Duvallet, Siavash Isazadeh, Chengzhen Dai, Shinkyu Park, Katya Frois-Moniz, Fabio Duarte, Carlo Ratti, Eric J. Alm, Fangqiong Ling
Summary: The metagenome embedded in urban sewage provides valuable information for understanding urban ecology and assessing human health status, and sewage samples can be used to estimate population sizes and monitor human diseases.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Plant Sciences
Xiaobing Wang, Yun Tang, Huiling Huang, Daidi Wu, Xiuzhen Chen, Junren Li, Hai Zheng, Ruoting Zhan, Likai Chen
Summary: In Pogostemon cablin, PcFPPS and its binding transcription factor PcWRKY44 play an essential role in regulating the biosynthesis of patchouli alcohol, a secondary metabolite in plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jia Wang, Ting Li, Mengling Li, Zhendong Fu, Likai Chen, Dongxing Shi, Fubin Qiu, Xintong Tan
Summary: Lycopene has been found to have beneficial effects on hepatic insulin signal transduction by alleviating mitochondrial dysfunction and up-regulating FGF21 expression under oxidative stress conditions.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Plant Sciences
Xiaobing Wang, Liting Zhong, Xuan Zou, Lizhen Gong, Jiexuan Zhuang, Danhua Zhang, Hai Zheng, Xiaomin Wang, Daidi Wu, Ruoting Zhan, Likai Chen
Summary: This study analyzed the metabolites of the aerial parts of Pogostemon cablin and identified 23 volatile compounds, mainly sesquiterpenes, using GC-MS. The highest level of patchouli alcohol was found in leaves. Non-volatile compounds of leaf, stem, and flower tissues were analyzed using UHPLC-QTOFMS, with the largest differences in metabolites observed between flower and leaf tissues. Tissue-specific biomarkers of metabolites were identified, and the differential metabolites were enriched in several KEGG pathways.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Yan Chen, Min Zhang, Likai Chen, Mengmeng Pan, Mingming Qin, Yanqiu Guo, Yaobo Zhang, Hao Pan, Yunlong Zhou
Summary: In this study, a self-combination strategy using zinc ions and clinically applied 5-aminolevulinic acid hydrochloride (ALA) was introduced to combat Staphylococcus epidermidis (S. epidermidis) infection and promote wound healing. The strategy significantly inhibited and eliminated biofilms through a synergistic mechanism, enhanced photodynamic inactivation, and aggravated cell wall/membrane disruption. Additionally, it accelerated wound repair and reduced inflammatory response without causing cytotoxicity.