Journal
DALTON TRANSACTIONS
Volume 42, Issue 10, Pages 3337-3345Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2dt32709k
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Funding
- MEXT [21550070]
- Chuo University
- Institute of Science & Engineering, Chuo University
- Grants-in-Aid for Scientific Research [21550070] Funding Source: KAKEN
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The binding site specificity of the ternary complexes, [M(II)(phen)(edda)] (M(II) = Pt2+ and Zn2+; phen = 1,10-phenanthroline; edda = N, N'-ethylenediaminediacetic acid), for the self-complementary oligonucleotides (ODNs), ds(C(1)G(2)C(3)G(4)A(5)A(6)T(7)T(8)C(9)G(10)C(11)G(12))(2) (ODN1) and ds(C(1)G(2)C(3)G(4)T(5)A(6)T(7)A(8)C(9)G(10)C(11)G(12))(2) (ODN2), was studied by NMR measurements. The results indicated that [Pt(II)(phen)(edda)] was partially intercalated between C-3/G(10) and G(4)/C-9 base pairs of ODN1 and ODN2 in the major grooves, whereas [Zn(II)(phen)(edda)] was bound specifically to the TATA region of ODN2 in the minor groove and to the terminal G(2)/C-11 base pair of ODN1 in the major groove. The preference for the TATA sequence over the AATT sequence in the binding of [Zn(phen)(edda)] was attributed to the wider minor groove width of the TATA sequence. The bindings of the complexes to ct-DNA were also studied by UV, CD, and fluorescence spectroscopy. Additionally, the antiproliferative property of [Pt(II)(phen)(edda)] towards MCF7 breast cancer cells and normal MCF10-A cells was compared with that of [Zn(II)(phen)(edda)].
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