Article
Pharmacology & Pharmacy
Victor Gustavo Balera Brito, Mariana Sousa Patrocinio, Ayna Emanuelli Alves Barreto, Sabrina Cruz Tfaile Frasnelli, Vanessa Soares Lara, Carlos Ferreira Santos, Sandra Helena Penha Oliveira
Summary: The study demonstrated that Telmisartan (TELM) inhibited osteogenic differentiation and mineralization of SHR BMSC, favoring an adipogenic phenotype due to Pparg upregulation.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Cell & Tissue Engineering
Changzhen Wang, Hongmei Ning, Jiao Gao, Teng Xue, Ming Zhao, Xiaoxia Jiang, Xiaoming Zhu, Ximin Guo, Hong Li, Xiaoyan Wang
Summary: This study investigated the impact of hematopoietic cells on MSCs and found that partial deletion of the Pten gene in hematopoietic cells can lead to abnormal differentiation of MSCs. There is a reciprocal relationship between hematopoietic cells and MSCs, with an important role in bone development.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Oliwia Milek, Dino Tur, Lucia Ahcin, Olha Voitseshyna, Christian Behm, Oleh Andrukhov
Summary: This study investigated the impact of osteogenic differentiation on the immunomodulatory properties of hPDL-MSCs, and found that osteogenic differentiation reduced the ability of hPDL-MSCs to suppress CD4(+) T lymphocyte proliferation and enhanced this ability in the presence of IL-1 beta. Additionally, the expression and activity of indoleamine-2,3-dioxygenase and interleukin-8 were also affected by the differentiation process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Laura Alves, Vanessa Machado, Joao Botelho, Jose Joao Mendes, Joaquim M. S. Cabral, Claudia L. da Silva, Marta S. Carvalho
Summary: Cell-based therapies using PDLSC may be a promising alternative to MSC(M) and MSC(AT) for periodontal regeneration, as PDLSC showed enhanced proliferative and osteogenic potential. PDLSC expressed osteogenic markers and had higher calcium content and upregulated osteogenic/periodontal genes compared to MSC(M) and MSC(AT). CD146 was specifically expressed by PDLSC, which also had a higher proliferative capacity. However, alkaline phosphatase activity did not increase in PDLSC.
Article
Biotechnology & Applied Microbiology
El-Mustapha Haddouti, Nina Reinhardt, Robert Ossendorff, Christof Burger, Dieter C. Wirtz, Matias de la Fuente, Frank A. Schildberg
Summary: Extracorporeal shock wave therapy is a non-invasive and effective treatment for musculoskeletal disorders. Research indicates that the optimal parameters for shock wave therapy are still undetermined. This study examined the effects of shock wave application on primary MSCs and osteoblastic cell lines in vitro, establishing conditions that enhance the osteogenic differentiation of MSCs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shuang Zhang, Jeroen van de Peppel, Marijke Koedam, Johannes P. T. M. van Leeuwen, Bram C. J. van der Eerden
Summary: In this study, TNS3 was found to be involved in the fate decision of human bone marrow-derived stromal cells (BMSCs), regulating osteogenesis and adipogenesis through the control of RhoA activity and cytoskeletal reorganization. The findings highlight the crucial role of TNS3 in bone metabolism.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Aleksandra Kostina, Arseniy Lobov, Daria Semenova, Artem Kiselev, Polina Klausen, Anna Malashicheva
Summary: Despite significant progress in bone tissue regeneration, the early mechanisms of osteogenic differentiation remain poorly understood. Various cells can undergo osteogenic transformation, with Notch signaling playing a key role in this process. Research demonstrates differences in the ability of different cell types to undergo Notch-dependent osteogenic differentiation.
Article
Pharmacology & Pharmacy
Ying Xing, Mi-Shuai Zhang, Jian-Hui Xiao, Ru-Ming Liu
Summary: This study found that galangin can promote the osteogenic differentiation of human amniotic mesenchymal stem cells (hAMSCs) through the JAK2/STAT3 signaling pathway.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Review
Cell Biology
Xiaoyang Liu, Zongke Zhou, Wei-Nan Zeng, Qin Zeng, Xingdong Zhang
Summary: Osteoimmunology involves crosstalk between the skeletal and immune systems. Toll-like receptors (TLRs) play a crucial role in bone remodeling by directly impacting MSC osteogenic differentiation. Understanding TLRs' role has significant implications for advancing bone regeneration and repair strategies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Jong Bo Park, Ji Yeon Ahn, Woo Sub Yang, Seong Chae Park, Young Jin Jung, Myungook Lee, Byung Hee Hong, Jeong Mook Lim
Summary: This study investigated the effect of nanoscale spacing by stacked graphene film on osteogenic differentiation of human adipose-derived mesenchymal stromal cells. The results showed that osteogenic differentiation was promoted as the stacking layer increased, with the most significant effects observed in the three layers. The study demonstrated that multilayer-stacked graphene created nanoscale spaces promote calcium deposit and cytoskeletal integrity in hADSC-related, in vitro-osteogenesis.
Article
Biology
Manali Jain, Neeta Singh, Raunaq Fatima, Aditya Nachanekar, Mandakini Pradhan, Soniya Nityanand, Chandra Prakash Chaturvedi
Summary: This study compares the growth and cardiomyogenic potential of AF-MSCs from congenital heart defective fetuses (ICHD) with normal fetuses. It finds that ICHD AF-MSCs have growth defects, increased senescence and DNA damage, and decreased expression of proteins necessary for heart development. These findings highlight the possible contribution of defects in AF-MSCs to congenital heart defects and improper heart development.
Article
Cell Biology
Marta S. Carvalho, Laura Alves, Isabel Bogalho, Joaquim M. S. Cabral, Claudia L. da Silva
Summary: The study found that aging impairs the proliferative and osteogenic capacities of MSC, but exposure to ECM derived from young cells can help rejuvenate aged MSC. The ECM plays a crucial role in the quantity and quality of MSC, especially in terms of osteogenic differentiation capacity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Environmental Sciences
Hana Najahi, Nicola Alessio, Tiziana Squillaro, Gea Oliveri Conti, Margherita Ferrante, Giovanni Di Bernardo, Umberto Galderisi, Imed Messaoudi, Sergio Minucci, Mohamed Banni
Summary: This study evaluated the effects of different size microplastics present in water bottles on human mesenchymal stromal cells. The results showed that microplastic treatments led to a decrease in cell proliferation, altered differentiation potential, and loss of osteocyte phenotype. This indicates that environmental microplastics can be bioavailable for cell uptake and may result in irreversible diseases.
ENVIRONMENTAL RESEARCH
(2022)
Article
Genetics & Heredity
Duojiao Chen, Sheng Liu, Xiaona Chu, Jill Reiter, Hongyu Gao, Patrick Mcguire, Xuhong Yu, Xiaoling Xuei, Yichen Liu, Jun Wan, Fang Fang, Yunlong Liu, Yue Wang
Summary: In this study, single-cell multiomic analysis was used to profile the transcriptome and epigenome of mesenchymal stem cells (MSC) from four healthy donors. The analysis revealed distinct chromatin accessible regulatory elements and differentiation potential among the MSC from different donors. The study also explored the enrichment of genetic variants associated with musculoskeletal diseases in the chromatin accessible regions, providing insights into the regulatory mechanisms of gene expression during MSC differentiation.
Article
Nanoscience & Nanotechnology
Ruyin Lyu, Yuping Chen, Yajun Shuai, Jie Wang, Leihao Lu, Qichao Cheng, Jiangfeng Cai, Chuanbin Mao, Mingying Yang
Summary: We demonstrate a universal strategy for discovering peptides that can bind to and functionalize biomaterials using a Bombyx mori silk fibroin membrane as a model. By fusing two peptides, one screened for binding to the biomaterial and another derived from a growth factor, a new chimeric peptide is created to efficiently bind to the silk fibroin membrane. The chimeric peptide enables the membrane to induce osteogenic differentiation of human mesenchymal stem cells and stimulate bone regeneration in a rat model without causing inflammation.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Virology
Giulia Mencattelli, Andrea Silverj, Federica Iapaolo, Carla Ippoliti, Liana Teodori, Annapia Di Gennaro, Valentina Curini, Luca Candeloro, Annamaria Conte, Andrea Polci, Daniela Morelli, Maria Gabriella Perrotta, Giovanni Marini, Roberto Rosa, Federica Monaco, Nicola Segata, Annapaola Rizzoli, Omar Rota-Stabelli, Giovanni Savini
Summary: This paper provides an overview of the epidemiological and genetic features of West Nile virus (WNV) Lineage 2 (L2) in Italy. The study reveals a progressive increase in WNV L2 in Italy and predicts a wider spread in the future. The findings emphasize the importance of using quantitative models for early warning detection of WNV outbreaks.
Article
Virology
Soufien Sghaier, Corinne Sailleau, Maurilia Marcacci, Sarah Thabet, Valentina Curini, Thameur Ben Hassine, Liana Teodori, Ottavio Portanti, Salah Hammami, Lucija Jurisic, Massimo Spedicato, Lydie Postic, Ines Gazani, Raja Ben Osman, Stephan Zientara, Emmanuel Breard, Paolo Calistri, Jurgen A. Richt, Edward C. Holmes, Giovanni Savini, Francesca Di Giallonardo, Alessio Lorusso
Summary: Epizootic haemorrhagic disease (EHD) is a viral disease transmitted by Culicoides and affects domestic and wild ruminants, particularly white-tailed deer and cattle. Recent outbreaks of EHDV-8 have been reported in cattle farms in central/western Tunisia, spreading rapidly throughout the country with more than 200 confirmed cases. This study emphasizes the need for vaccines targeting various serotypes of EHDV.
Article
Biochemistry & Molecular Biology
Alessia Peserico, Chiara Di Berardino, Giulia Capacchietti, ChiaraCamerano Spelta Rapini, Liliana Liverani, Aldo Roberto Boccaccini, Valentina Russo, Annunziata Mauro, Barbara Barboni
Summary: Comparing with the validated cumulus-oocyte complex (COC) protocol, a three-dimensional (3D) scaffold-mediated follicle-enclosed oocytes (FEO) system was found to optimize in vitro maturation (IVM) for ovine early antral (EA)-derived oocytes. By combining hCG stimulation with ovarian surface epithelium (OSE) cells coculture exclusively on the FEO system, the maturation rate and parthenogenetic activation of EA-derived oocytes were significantly improved. The paracrine factors released specifically from OSE enhanced the hCG-triggering of oocyte maturation mechanisms by acting through the mural compartment, stimulating the EGFR signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Alessia Peserico, Barbara Barboni, Valentina Russo, Delia Nardinocchi, Maura Turriani, Costanza Cimini, Nicola Bernabo, Ornella Parolini, Antonietta Rosa Silini, Ivana Antonucci, Liborio Stuppia, Paolo Berardinelli, Ilaria Falanga, Davide Perruzza, Luca Valbonetti, Annunziata Mauro
Summary: Amniotic membrane and amniotic fluid derived cells show potential as stem cell sources for regenerative medicine in male infertility diseases. This study examined the effects of two cell sources, hAFMSCs and hAECs, on male fertility outcomes in a rat VAR model. The results demonstrated that hAECs were more effective in restoring fertility rate by enhancing both structural and immune response mechanisms, while hAFMSCs contributed to different mechanisms leading to testis homeostasis. These findings propose innovative stem-based regenerative medicine protocols for male infertility conditions such as VAR.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Moussa Moise Diagne, Marie Henriette Dior Ndione, Giulia Mencattelli, Amadou Diallo, El Hadji Ndiaye, Marco Di Domenico, Diawo Diallo, Mouhamed Kane, Valentina Curini, Ndeye Marieme Top, Maurilia Marcacci, Maimouna Mbanne, Massimo Ancora, Barbara Secondini, Valeria Di Lollo, Liana Teodori, Alessandra Leone, Ilaria Puglia, Alioune Gaye, Amadou Alpha Sall, Cheikh Loucoubar, Roberto Rosa, Mawlouth Diallo, Federica Monaco, Ousmane Faye, Cesare Camma, Annapaola Rizzoli, Giovanni Savini, Oumar Faye
Summary: This study developed and validated a new amplicon-based sequencing approach for West Nile virus, which can effectively control the diffusion of different lineages. It has great potential for genomic surveillance of the virus.
Article
Microbiology
Monica Giammarioli, Dondo Alessandro, Cesare Camma, Loretta Masoero, Claudia Torresi, Maurilia Marcacci, Simona Zoppi, Valentina Curini, Antonio Rinaldi, Elisabetta Rossi, Cristina Casciari, Michela Pela, Claudia Pellegrini, Carmen Iscaro, Francesco Feliziani
Summary: African swine fever (ASF) has significant socio-economic effects in the global pig industry, particularly for countries with large-scale piggery sectors. In January 2022, the ASF virus genotype II was found in wild boars in mainland Italy. This study characterized the molecular features of the ASFV isolates and found a close genetic correlation with strains from Eastern/Central European and Asian countries. The Italian ASFV strains belong to the major CVR variant and have similarities with strains identified in wild boars and domestic pigs.
Article
Public, Environmental & Occupational Health
Alessio Di Lorenzo, Iolanda Mangone, Patrizia Colangeli, Daniela Cioci, Valentina Curini, Giacomo Vincifori, Maria Teresa Mercante, Adriano Di Pasquale, Simona Iannetti
Summary: The Istituti Zooprofilattici Sperimentali (IZSs) were appointed by the Italian Ministry of Health to carry out diagnostic tests for COVID-19. The IZS-Teramo conducted tests using Real Time RT-PCR on nasopharyngeal swabs, and developed bioinformatics workflows to monitor virus variants.
Article
Microbiology
Monica Giammarioli, Maurilia Marcacci, Maria Teresa Scicluna, Antonella Cersini, Claudia Torresi, Valentina Curini, Massimo Ancora, Antonio Rinaldi, Marcello Giovanni Sala, Elisabetta Rossi, Cristina Casciari, Michela Pela, Claudia Pellegrini, Carmen Iscaro, Cesare Camma, Francesco Feliziani
Summary: In April 2022, the complete genome sequence of African swine fever virus (ASFV) genotype II, strain 20355/RM/2022_Italy, was identified in a wild boar in the city of Rome, Italy.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Materials Science, Biomaterials
Marina Ramal-Sanchez, Felice Lorusso, Angela Taraschi, Luca Valbonetti, Nicola Bernabo, Calogero Bugea, Antonio Scarano
Summary: This scientometric study analyzed all the research papers published between 1990 and 2021 that included the keyword "zygomatic implants". The data showed a rapid increase in publications and citations per year, with the USA being the most productive country in this field. The analysis of keywords revealed zygomatic fractures as the most common word and complications as the most recent keyword.
Article
Virology
Sarah Thabet, Soufien Sghaier, Thameur Ben Hassine, Darine Slama, Raja Ben Osmane, Ridha Ben Omrane, Wiem Mouelhi, Massimo Spedicato, Alessandra Leone, Liana Teodori, Valentina Curini, Moncef Othmani, Shadia Berjaoui, Paola Ripa, Makram Orabi, Bassem Belhaj Mohamed, Ayda Sayadi, Sonia Ben Slama, Maurilia Marcacci, Giovanni Savini, Alessio Lorusso, Salah Hammami
Summary: EHDV-8 was found in deer carcasses in the national reserve of El Feidja, Jendouba, Northwestern Tunisia in 2021 and 2022. Insect vectors carrying EHDV-8, mainly Culicoides kingi and Culicoides oxystoma species, were collected in the areas surrounding Tozeur city in Southern Tunisia.
Article
Multidisciplinary Sciences
Valeria Di Lollo, Angelo Canciello, Alessia Peserico, Massimiliano Orsini, Valentina Russo, Adrian Cervero-Varona, Beatrice Dufrusine, Mohammad El Khatib, Valentina Curini, Annunziata Mauro, Paolo Berardinelli, Cathy Tournier, Massimo Ancora, Cesare Camma, Enrico Dainese, Luana Fiorella Mincarelli, Barbara Barboni
Summary: This study reveals the impact of epithelial-mesenchymal transition (EMT) on the immune properties of amniotic epithelial cells (AECs). The response to lipopolysaccharide differs between pre-EMT (eAECs) and post-EMT (mAECs), with eAECs upregulating genes involved in antigen-presenting response and mAECs over-expressing soluble inflammatory mediator transcripts. Network analysis identifies CIITA and Nrf2 as the main drivers of eAECs and mAECs immune response, respectively.