Article
Pharmacology & Pharmacy
Yoshiki Ando, Fei-Chien Chang, Matthew James, Yang Zhou, Miqin Zhang
Summary: In this study, three-dimensional chitosan scaffolds are developed as microcarriers for the prolonged expansion of human neural stem cells (hNSCs) in bioreactors. The chitosan scaffolds exhibit desirable microstructural characteristics and mechanical properties, allowing for hNSC expansion in dynamic conditions for up to three weeks. This microcarrier approach has the potential for mass production of hNSCs in a three-dimensional setting.
Article
Biochemistry & Molecular Biology
Christina McKee, Christina Brown, Shreeya Bakshi, Keegan Walker, Chhabi K. Govind, G. Rasul Chaudhry
Summary: The use of 3-D self-assembling scaffolds supports the maintenance of naive state characteristics of ESCs and long-term growth. Gene expression profiles and biological processes in 3-D grown cells show significant changes, with upregulation of genes associated with transcription and translation regulation, extracellular matrix organization, and chromatin remodeling. Additionally, upregulation of genes linked to Wnt and focal adhesion signaling, and downregulation of genes related to the p53 signaling pathway, provide insight into the possible mechanisms of self-renewal of naive ESCs stimulated by mechanical signals in the 3-D microenvironment.
Article
Biotechnology & Applied Microbiology
Despoina Nektaria Metsiou, Foteini K. Kozaniti, Despina D. Deligianni
Summary: The study investigated the effects of hUMSCs-CM treatment on the biomechanical characteristics of ER+ and HER2+ breast cancer cells, with positive effects observed in ER+ cells but not in HER2+ cells. Post-treatment, ER+ cells showed limited cell viability and migratory potential alongside increased cell stiffness, while HER2+ cells exhibited increased cell viability and decreased stiffness indicating higher potential for cell movement.
BIOENGINEERING-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Sang-Hoon Yoon, Mi-Rae Bae, Hyeonwoo La, Hyuk Song, Kwonho Hong, Jeong-Tae Do
Summary: This study proposed an efficient method for deriving NSCs from mouse ESCs through a combination of 3D culture for early neural lineage commitment and 2D NSC derivation. The results demonstrated that ESCs could efficiently differentiate into tripotent NSCs through specification in 3D culture followed by 2D culture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Tingting Gao, Xiyuan Zhao, Jie Hao, Yao Tian, Huike Ma, Wenjing Liu, Bin An, Faguo Sun, Shasha Liu, Baojie Guo, Shuaishuai Niu, Zhongwen Li, Chenxin Wang, Yukai Wang, Guihai Feng, Liu Wang, Wei Li, Jun Wu, Meijin Guo, Qi Zhou, Qi Gu
Summary: In this study, a new microcarrier with hyaluronic acid (HA) as the core material was developed for scalable serum-free suspension culture of hESC-derived MSCs. The optimal microcarriers, with a coating collagen concentration of 100 μg/mL or concave-structured surface (cHAMCs), allowed for the expansion of IMRCs within six days with a high yield. The harvested cells showed high viability, immunomodulatory and regenerative therapeutic promise, as well as increased secretion of extracellular matrix (ECM).
MATERIALS TODAY BIO
(2023)
Article
Cell Biology
Mingming Zhang, Yuerong Xu, Yan Chen, Qinru Yan, Xiaoli Li, Lu Ding, Ting Wei, Di Zeng
Summary: This study explored the effects of restructuring iPSC-CMs in 3D nanofibrous scaffolds on cell morphology, cardiac-specific structural protein, gap junction, and calcium transient kinetics, and found that this treatment enhanced the maturation of iPSC-CMs. The results suggest that electrospun 3D PCL nanofibrous scaffolds can be a potential strategy to improve myocardium bioengineering by combining stem cells and scaffolds.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Anjana K. Badekila, Sudarshan Kini, Amit K. Jaiswal
Summary: 3D cell culture models provide more accurate simulation of in vivo environments, offering insights into various cellular processes. The design of appropriate scaffolds is crucial for organizing the cell microenvironment. The diversification of polymeric scaffolds and advancements in fabrication techniques have significant applications in tissue engineering, drug screening, and tumor model development.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Chemistry, Physical
Xin Li, Honglei Jian, Qingquan Han, Anhe Wang, Jieling Li, Ningyuan Man, Qi Li, Shuo Bai, Junbai Li
Summary: Short peptide self-assembled hydrogels have excellent biocompatibility and functional expansion, making them promising in cell culture and tissue engineering. However, the preparation of bio-inks with adjustable mechanical strength and controlled degradation for 3D bioprinting is challenging.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Engineering, Environmental
Wenting Yu, Xiaoxia Su, Moxiao Li, Wanting Wan, Ang Li, Hong Zhou, Feng Xu
Summary: This study investigated the effects of matrix strain on the characteristics and bioactivity of MSC-derived exosomes by culturing PDLSCs in three-dimensional magnetically stretched collagen hydrogels. The results showed that altering the miRNA content in exosomes significantly enhanced their bioactivity, leading to improved repair of bone defects in vivo. This research suggests a novel strategy for promoting tissue regeneration through engineered mechanical microenvironments.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Engineering, Biomedical
Yan Xie, Linawati Sutrisno, Toru Yoshitomi, Naoki Kawazoe, Yingnan Yang, Guoping Chen
Summary: Interconnected scaffolds promote the chondrogenic differentiation of stem cells. In this study, collagen scaffolds with interconnected pore structures were fabricated using PLGA sponge templates and were used to culture human bone marrow-derived mesenchymal stem cells (hMSCs). The results showed that the interconnected scaffolds can enhance the chondrogenic differentiation of stem cells and improve the production of cartilaginous matrices and the mechanical properties of the implants after subcutaneous implantation.
BIOMEDICAL MATERIALS
(2022)
Article
Engineering, Biomedical
Monika Wasyleczko, Zuzanna Joanna Krysiak, Ewa Lukowska, Marcin Gruba, Wioleta Sikorska, Aleksandra Kruk, Judyta Dulnik, Jaroslaw Czubak, Andrzej Chwojnowski
Summary: The reconstruction of cartilage tissue is challenging due to its lack of blood supply and innervation. This paper explores the characteristics and treatment strategies for cartilage diseases, highlighting the use of scaffolds and human cells for regeneration. The comparison of different biomaterials, specifically poly(L-lactide) (PLLA) and polyethersulfone (PES) membranes, reveals that PES scaffolds have higher cell activity and protein content.
BIOCYBERNETICS AND BIOMEDICAL ENGINEERING
(2022)
Article
Cell Biology
Sebastian Kress, Roland Schaller-Ammann, Juergen Feiel, Joachim Wegener, Joachim Priedl, Wolf Dietrich, Cornelia Kasper, Dominik Egger
Summary: The use of 3D cell cultures is becoming increasingly important in medical and pharmaceutical research. However, analyzing the culture medium alone is not representative of the culture conditions in a 3D model, which hinders standardization and translation of results. Researchers have developed a monitoring platform that combines a perfusion bioreactor with a minimally invasive sampling system and sensors to monitor culture parameters and medium compounds in 3D cultures.
Article
Biotechnology & Applied Microbiology
Asher Kahn-Krell, Danielle Pretorius, Bijay Guragain, Xi Lou, Yuhua Wei, Jianhua Zhang, Aijun Qiao, Yuji Nakada, Timothy J. Kamp, Lei Ye, Jianyi Zhang
Summary: Researchers introduce a simple and reproducible 3D culture method for generating cardiac spheroids, which contain cardiomyocytes, vascular cells and cardiac fibroblasts. This method promotes cardiomyocyte maturation and improves their bioenergetics.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Benson Koh, Nadiah Sulaiman, Mh Busra Fauzi, Jia Xian Law, Min Hwei Ng, Too Lih Yuan, Abdul Ghani Nur Azurah, Mohd Heikal Mohd Yunus, Ruszymah Bt Hj Idrus, Muhammad Dain Yazid
Summary: This study aimed to explore the potential of xeno-free three-dimensional cultures for mesenchymal stem cell (MSCs) expansion in clinical applications. Human serum and human platelet lysate were found to be suitable alternatives to replace foetal bovine serum in MSCs microcarrier cultures. Low glucose DMEM (LG) + human platelet (HPL) lysate was identified as the best xeno-free culture media, promoting high cell yield and maintaining MSC characteristics. This culture system showed improved immunomodulatory activities, suggesting its potential for future clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Amparo Guerrero Gerboles, Maricla Galetti, Stefano Rossi, Francesco Paolo lo Muzio, Silvana Pinelli, Nicola Delmonte, Cristina Caffarra Malvezzi, Claudio Macaluso, Michele Miragoli, Ruben Foresti
Summary: The toxicity of nanoparticles absorbed through contact or inhalation is a major concern for public health. In vitro nanotoxicological studies are conventionally limited, but the use of 3D bioprinting can provide a new perspective. In this study, 3D organoid-based scaffolds were developed to simulate lung cell exposure to nanoparticles, demonstrating toxicology responses that recapitulate in vivo experiments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)