Article
Cell & Tissue Engineering
Takeshi Watanabe, Satoshi Yasuda, Shinji Kusakawa, Takuya Kuroda, Mayumi Futamura, Mitsuhide Ogawa, Hidemi Mochizuki, Eri Kikkawa, Hatsue Furukawa, Masato Nagaoka, Yoji Sato
Summary: The MEASURE study aims to standardize the tumorigenicity evaluation of hPSC-derived CTPs, finding that the HEC assay and MACS can improve sensitivity to residual undifferentiated hPSCs. Multisite experiments confirmed the effectiveness of these methods in detecting low levels of undifferentiated cells.
Review
Biochemistry & Molecular Biology
Bo Fu, Hong Ma, Di Liu
Summary: During fertilization and embryo development, the cellular totipotency undergoes changes, with some embryonic stem cells being able to convert to a totipotent state. In the process of somatic cell nuclear transfer (SCNT), however, the establishment of totipotency in cloned embryos is often compromised. This review explores practical strategies for inducing 2-cell-like cells (2CLCs) in the study of cloned embryo development and suggests that these cell models can offer new opportunities for improving SCNT efficiency.
Review
Medicine, General & Internal
Paula Salmikangas, Bjorn Carlsson, Christophe Klumb, Tatiana Reimer, Steffen Thirstrup
Summary: Potency is a critical quality attribute of biological medicinal products that reflects their biological activity. For timely release and comparability studies, quantitative in vitro assays are necessary. Cell and Gene Therapy Products (CGTs) present challenges in potency testing due to their complexity.
FRONTIERS IN MEDICINE
(2023)
Review
Immunology
Chiara Capelli, Carolina Cuofano, Chiara Pavoni, Simona Frigerio, Daniela Lisini, Sara Nava, Michele Quaroni, Valentina Colombo, Francesco Galli, Svetlana Bezukladova, Paola Panina-Bordignon, Giuseppe Gaipa, Patrizia Comoli, Giulio Cossu, Gianvito Martino, Andrea Biondi, Martino Introna, Josee Golay
Summary: Advanced Therapy Medicinal Products (ATMPs) based on somatic cells expanded in vitro, with or without genetic modification, is a rapidly growing area of drug development. Potency assays are crucial for assessing the quality of ATMPs, and biomarkers may serve as substitutes for complex functional potency tests in predicting the efficacy of these cell-based drugs in vivo.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Environmental Sciences
R. L. Putland, C. A. F. de Jong, B. Binnerts, A. Farcas, N. D. Merchant
Summary: This study conducted a multi-site validation of monthly and annual shipping noise maps for 2019 produced as part of the JOMOPANS program, and analyzed the differences between predictions and measurements. Validating shipping noise models ensures their confident application in future management decisions.
MARINE POLLUTION BULLETIN
(2022)
Article
Multidisciplinary Sciences
Jani Huuhtanen, Dipabarna Bhattacharya, Tapio Lonnberg, Matti Kankainen, Cassandra Kerr, Jason Theodoropoulos, Hanna Rajala, Carmelo Gurnari, Tiina Kasanen, Till Braun, Antonella Teramo, Renato Zambello, Marco Herling, Fumihiro Ishida, Toru Kawakami, Marko Salmi, Thomas Loughran, Jaroslaw P. Maciejewski, Harri Lahdesmaki, Tiina Kelkka, Satu Mustjoki
Summary: In T cell large granular lymphocytic leukemia (T-LGLL), the non-leukemic immune system is interconnected with the T-LGLL clonotype(s) and exhibits a more mature, cytotoxic, and clonally restricted T cell repertoire compared to other cancers and autoimmune disorders. Moreover, the T-LGLL clonotypes are more cytotoxic and exhausted than healthy reactive clonotypes, and they actively communicate with non-leukemic immune cells through various mechanisms. 72% of the leukemic T-LGLL clonotypes share T cell receptor similarities with their non-leukemic repertoire, suggesting possible common target antigens.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Dennis M. Timmerman, Tessa L. Remmers, Sanne Hillenius, Leendert H. J. Looijenga
Summary: The P53 pathway is crucial for maintaining genomic and cellular integrity, with disruptions leading to cell senescence or apoptosis. Mutations in TP53 are common in solid tumors, while other factors like ARF, ANRIL, TRIM proteins, miRNAs, and CTCF can also deregulate P53 signaling. Understanding these factors can potentially lead to new targets for cancer therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Beatriz Fernandez-Munoz, Ana Belen Garcia-Delgado, Blanca Arribas-Arribas, Rosario Sanchez-Pernaute
Summary: Neural stem cells are attractive for regenerative therapies and are being tested in clinical trials for neurological disorders. Different sources of neural stem cells have advantages and disadvantages, with ethical concerns surrounding embryonic sources. Further research is needed to improve efficiency and characterization of alternative sources.
Article
Biotechnology & Applied Microbiology
Valeria Peli, Mario Barilani, Araceli Rivera-Ordaz, Paolo Manzini, Francesco Rusconi, Cristiana Lavazza, Silvia Cimoni, Lorenza Lazzari
Summary: This study validated an image cytometer-based method for determining the viability of human induced pluripotent stem cells (hiPSCs) and demonstrated its feasibility as a quality control test during large-scale GMP hiPSC expansion.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Bruno Navarro, Paula Elbl, Leandro F. de Oliveira, Amanda R. Piovezani, Andre L. W. dos Santos, Diego T. de Souza, Diego Demarco, Marcos S. Buckeridge, Eny I. S. Floh
Summary: The study investigated the cell-to-cell communication in cell cultures with different embryogenic potential in Araucariaangustifolia. It identified differences in protein abundance related to vesicular transport and cell-to-cell communication processes, with a callose hydrolase showing differential expression between the two cell lines. Immunocytochemical and Transmission Electron Microscopy analyses supported the finding of heightened vesicular activity in the Responsive cell line. Hence, vesicular transport and plasmodesmata regulation features play a crucial role in somatic embryogenic responsiveness in Araucariaangustifolia.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Green & Sustainable Science & Technology
Francielle Carvalho, Eduardo Muller-Casseres, Matheus Poggio, Tainan Nogueira, Clarissa Fonte, Huang Ken Wei, Joana Portugal-Pereira, Pedro R. R. Rochedo, Alexandre Szklo, Roberto Schaeffer
Summary: The study evaluates 14 fuel options, finding that Fischer-Tropsch diesel, alcohol-based diesel, vegetable oils, and e-diesel are promising mid-term alternatives, while Bio-LNG is hampered by the risk of methane slip. Green hydrogen and green ammonia may not be competitive alternatives for Brazil in the mid-term but could become alternatives for cabotage transport in the long term.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Xingdong Wang, Jie Pei, Lin Xiong, Shaoke Guo, Mengli Cao, Yandong Kang, Ziqiang Ding, Yongfu La, Chunnian Liang, Ping Yan, Xian Guo
Summary: This study conducted a comprehensive and unbiased single-cell transcriptomic analysis of spermatogenesis in sexually mature 4-year-old yaks using scRNA-seq. The analysis revealed the presence of six somatic cell types and various germ cells in yak testis. Functional enrichment analysis showed that highly expressed genes in yak testicular somatic cells were significantly enriched in cAMP signaling pathway, PI3K-Akt signaling pathway, MAPK signaling pathway, and ECM receptor interactions. Immunohistochemical techniques identified candidate marker genes for spermatogonial stem cells and Sertoli cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cardiac & Cardiovascular Systems
Doris A. Taylor, Lourdes Chacon-Alberty, Luiz C. Sampaio, Mariana Gonzalez Del Hierro, Emerson C. Perin, Fernanda C. P. Mesquita, Timothy D. Henry, Jay H. Traverse, Carl J. Pepine, Joshua M. Hare, Michael P. Murphy, Phillip C. Yang, Keith L. March, Rachel W. Vojvodic, Ray F. Ebert, Roberto Bolli
Summary: Cell-based therapy lacks standardized definitions and terminology, making comparisons across studies challenging. This article discusses the lack of consistency in descriptive terminology used in cell-based therapies and provides guidelines for standardizing nomenclature and definitions.
CARDIOVASCULAR RESEARCH
(2022)
Article
Transportation
Yi Xiao, Zhuo Chen, Levi McNeil
Summary: With smart shipping still at its preliminary stage and lacking related literature, it is necessary to establish an index system for development. Smart shipping consists of four main domains: smart waterways, smart ports, smart ships, and maritime intelligence, with experts agreeing that ports are crucial for achieving smart shipping. High-scoring indexes within the system can aid stakeholders in making decisions related to smart shipping participation.
MARITIME POLICY & MANAGEMENT
(2022)
Article
Cell & Tissue Engineering
Liam Chung, Amarin Cogburn, Lina Sui, Jennifer L. Dashnau
Summary: Allogeneic cell therapy platforms derived from induced pluripotent stem cells (iPSCs) have gained popularity due to limitations of autologous cell therapy. However, the risk of residual undifferentiated cells (iPSCs) associated with teratoma formation needs to be addressed. A sensitive miRNA-based assay was developed to detect residual iPSCs in differentiated cell samples.
