Article
Biology
Ye-Jin Jeong, Kwang-Il Kim
Summary: A new cell line, DGF, was established from the dwarf gourami, which is highly susceptible to RSIV and ISKNV. These cells can be used for viral diagnosis and studying the pathogenesis of ISKNV.
Article
Fisheries
Cahya Fusianto, Paul M. Hick, Murwantoko, Afri Herlambang, Richard J. Whittington, Joy A. Becker
Summary: A diagnostic study was conducted on mass mortality events in hybrid groupers in sea cages off the northern coast of Bali in 2016. The study revealed a high prevalence of NNV infection, with no significant differences between fish with and without clinical signs. MCV infection was also prevalent, with a significant proportion of clinically affected fish showing pathology consistent with ISKNV infection.
AQUACULTURE REPORTS
(2021)
Article
Microbiology
Weixuan Fu, Yong Li, Yuting Fu, Wenfeng Zhang, Panpan Luo, Qianqian Sun, Fangzhao Yu, Shaoping Weng, Wangdong Li, Jianguo He, Chuanfu Dong
Summary: Red seabream iridovirus (RSIV) is a major cause of economic loss in mariculture bony fish worldwide. Previous studies have shown that the diversity of RSIV isolates can affect their virulence, antigenicity, and vaccine efficacy. This study demonstrated that ISKNV-I-based inactivated vaccine can provide almost complete cross-protection against RSIV-I and RSIV-II, as well as ISKNV-I itself.
MICROBIOLOGY SPECTRUM
(2023)
Article
Genetics & Heredity
Samantha A. Koda, Kuttichantran Subramaniam, Deborah B. Pouder, Roy P. Yanong, Salvatore Frasca, Vsevolod L. Popov, Thomas B. Waltzek
Summary: The study reports the complete genome sequences of two megalocytiviruses isolated from diseased albino rainbow sharks reared on farms in the United States in 2018 and 2019, confirming the presence of ISKNV in albino rainbow sharks for the first time. Phylogenomic analyses revealed that both EFIV isolates belong to the ISKNV genotype within the genus Megalocytivirus.
Article
Fisheries
Thangaraj Raja Swaminathan, Nithianantham Sundar Raj, Prasannan Geetha Preena, Pravata Kumar Pradhan, Neeraj Sood, Rahul Girish Kumar, Arun Sudhagar, Naresh Kumar Sood
Summary: This study isolated ISKNV from diseased giant gourami in India, confirming its pathogenicity. The research highlights the importance of implementing stringent biosecurity measures to control the disease.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Youhua Huang, Liwei Huang, Ya Zhang, Qi Zheng, Liwen Xu, Qiwei Qin, Xiaohong Huang
Summary: We established a new cell line from sea perch fry, which predominantly consisted of epithelial-like cells and proliferated well under specific culture conditions. These SPF cells showed susceptibility to different viruses and underwent non-apoptotic cell death upon infection. Additionally, interferon-related genes were up-regulated in virus-infected SPF cells, indicating the involvement of host interferon immune response in virus infection.
Article
Fisheries
Hengwei Deng, Yeyu Li, Jinling Li, Wenjie Shen, Qiankang Chen, Shaoping Weng, Jianguo He, Xiaopeng Xu
Summary: Neomycin can inhibit ISKNV infection by antagonizing the shift of cellular redox balance and down-regulating the expression of major capsid protein. The current study suggests that targeting the viral-induced redox imbalance can be used as a therapeutic strategy for Megalocytivirus diseases.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Yinjie Niu, Xiaozhe Fu, Qiang Lin, Lihui Liu, Xia Luo, Hongru Liang, Ningqiu Li
Summary: The study demonstrated that gefitinib could effectively inhibit Infectious spleen and kidney necrosis virus (ISKNV) infection by blocking virus entry and corresponding protein synthesis.
Article
Fisheries
Akapon Vaniksampanna, Orapan Manajit, Saengchan Senapin, Suphattra Kamsamarn, Pradit Wangman, Siwapon Longyant, Parin Chaivisuthangkura
Summary: In this study, specific monoclonal antibodies (MAbs) against the major capsid protein (MCP) of Infectious spleen and kidney necrosis virus (ISKNV) were generated. These MAbs showed high sensitivity and specificity in detecting the virus in fish and could potentially be used for the detection of other related viruses.
Article
Infectious Diseases
Jose Gustavo Ramirez-Paredes, Richard K. Paley, William Hunt, Stephen W. Feist, David M. Stone, Terence R. Field, David J. Haydon, Peter A. Ziddah, Mary Nkansa, James Guilder, Joshua Gray, Samuel Duodu, Emanuel K. Pecku, Joseph A. Awuni, Timothy S. Wallis, David W. Verner-Jeffreys
Summary: The study identified ISKNV as the primary cause of fish mortality in tilapia aquaculture farms around Lake Volta in Ghana, with the disease being endemic by July 2019. Additionally, the absence of TiLV and nodavirus in the samples suggests a different disease mechanism.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Fisheries
Yuting Fu, Yong Li, Xiangrong Liu, Weixuan Fu, Shaoping Weng, Fangzhao Yu, Jianguo He, Chuanfu Dong
Summary: In this study, two novel cell lines derived from yellowfin seabream were established and characterized, providing important information for understanding the infection characteristics of Scale drop disease virus (SDDV).
Article
Fisheries
Zhao Zhao, Rui Ma, Zi-Rao Guo, Chen Zhang, Yan Xiong, Gao-Xue Wang, Bin Zhu
Summary: By using single-walled carbon nanotubes as drug carriers, the study successfully enhanced the antiviral effect of moroxydine hydrochloride against infectious spleen and kidney necrosis virus in mandarin fish, reducing the therapeutic dosage and extending the drug retention time in the body.
Article
Fisheries
Liming Xu, Jingzhuang Zhao, Miao Liu, Guangming Ren, Shi Cheng Han, Yizhi Shao, Yongsheng Cao, Hongbai Liu, Tongyan Lu
Summary: IHNV enhances the multiplication of IPNV during co-infection, with the enhancement becoming stronger with increasing infection time interval, regardless of the infection order.
Article
Fisheries
Zhao Zhao, Yang Li, Guo Chen, Chen Zhang, Gao-Xue Wang, Bin Zhu
Summary: Infectious spleen and kidney necrosis virus (ISKNV) has caused significant economic losses on mandarin fish in China due to its wide host range and high mortality rate. Vaccines are considered one of the most effective measures to control virus infection as there are no commercial drugs available for curing ISKNV. However, the weak immunogenicity of antigen protein in subunit vaccines often requires the use of boosters, adjuvants, or carriers to ensure strong and long-lasting protective immunity. This study demonstrated that using single-walled carbon nanotubes (SWCNTs) as carriers and mannose (M) as targeting factors in a main capsid protein (MCP) subunit vaccine (SWCNTs-M-MCP) resulted in rapid and strong antibody responses, significantly higher than those of the MCP and SWCNTs-MCP groups. These findings suggest that SWCNTs and mannose can enhance the immune response of vaccines and may provide an effective strategy against ISKNV.
AQUACULTURE RESEARCH
(2022)
Article
Fisheries
Min Zhu, Zeen Shen, Yuchao Gu, Xinyu Tong, Yaxin Zhang, Jun Pan, Yongjie Feng, Xiaolong Hu, Yujun Wang, Guangli Cao, Renyu Xue, Chengliang Gong
Summary: This study developed a live-vector vaccine based on baculovirus technology to prevent diseases caused by highly pathogenic ISKNV infection. The vaccine successfully delivered and expressed the major capsid protein gene in fish cells and tissues, inducing expression of immune-related genes. ELISA results showed the production of specific antibodies in vaccinated fish. Injection and immersion vaccination trials demonstrated good immune efficacy in fish of different sizes.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Z. M. Zhu, C. F. Dong, S. P. Weng, J. G. He
JOURNAL OF FISH DISEASES
(2018)
Article
Microbiology
Chuanfu Dong, Zhimei Wang, Shaoping Weng, Jianguo He
VETERINARY MICROBIOLOGY
(2017)
Article
Fisheries
Zhiming Zhu, Chuanfu Dong, Shaoping Weng, Jianguo He
FISH & SHELLFISH IMMUNOLOGY
(2019)
Article
Fisheries
Wenfeng Zhang, Chao Duan, Hetong Zhang, Shaoping Weng, Jianguo He, Chuanfu Dong
Article
Fisheries
Zhiming Zhu, Chao Duan, Chuanfu Dong, Shaoping Weng, Jianguo He
Article
Immunology
Yujia Sun, Zhiming Zhu, Shaoping Weng, Jianguo He, Chuanfu Dong
MICROBIAL PATHOGENESIS
(2020)
Article
Fisheries
Zhiming Zhu, Chao Duan, Yong Li, Congling Huang, Shaoping Weng, Jianguo He, Chuanfu Dong
Summary: This study provides detailed evidence confirming that ISKNV-II is the causative agent of severe diseases in farmed Asian seabass in mainland China. The research contributes to the pathogenic and epidemiological studies of Asian seabass iridovirus disease in mainland China and other Asian countries and regions. Various detection methods such as histopathology, immunohistochemistry, and transmission electron microscope were used to identify abnormal enlarged cells and viral particles in infected tissues.
Article
Virology
Yuting Fu, Yong Li, Weixuan Fu, Huibing Su, Long Zhang, Congling Huang, Shaoping Weng, Fangzhao Yu, Jianguo He, Chuanfu Dong
Summary: The study describes the prevalence of Scale drop disease virus (SDDV) in farmed Asian seabass in Southeast Asia, isolates an SDDV ZH-06/20, and analyzes its genome and proteome. SDDV ZH-06/20 was found to cause 100% mortality in juvenile yellowfin seabream, characterized by severe ascites. This study provides valuable information for better understanding SDDV-associated diseases in farmed fish.
Article
Fisheries
Fengjun Liu, Shaoping Weng, Jianguo He, Chuanfu Dong
Summary: This study revealed the involvement of CypA protein in CyHV-3 replication, as it interacts with the viral envelope protein VP32. Inhibition of CypA reduces viral proliferation, while overexpression enhances it.
Article
Fisheries
Hetong Zhang, Hemei Qi, Shaoping Weng, Jianguo He, Chuanfu Dong
Summary: In this study, the virulence-related gene ORF71L of Infectious spleen and kidney necrosis virus (ISKNV) was identified. Deletion of ORF71L resulted in decreased viral replication rate, reduced virulence, and lower mortality in mandarin fish. Additionally, deletion of ORF71L also led to an increase in the number of melanomacrophage centers in the spleen of infected fish.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Yujia Sun, Shaoping Weng, Binfu Xu, Chuanfu Dong, Jianguo He
Summary: Pseudomonas plecoglossicida infection causes a high probability (62%) of visceral white spot disease in orange-spotted grouper. After infection, the spleen mass increases and the liver mass decreases. Diseased fish suffer from anemia, hypoxia, and iron loss.
Article
Fisheries
Yuting Fu, Yong Li, Xiangrong Liu, Weixuan Fu, Shaoping Weng, Fangzhao Yu, Jianguo He, Chuanfu Dong
Summary: In this study, two novel cell lines derived from yellowfin seabream were established and characterized, providing important information for understanding the infection characteristics of Scale drop disease virus (SDDV).
Article
Fisheries
Wenfeng Zhang, Hengwei Deng, Yuting Fu, Weixuan Fu, Shaoping Weng, Jianguo He, Chuanfu Dong
Summary: This study successfully produced and characterized monoclonal antibodies (mAbs) against Mandarinfish ranavirus (MRV). Seven mAbs that specifically recognized MRV/LMBV were obtained through Western blotting screening and they targeted late expression structural proteins of MRV. The tissue tropism of MRV was re-assessed using immuno-histochemistry and immuno-histo-fluorescence, and it was found that spleen, kidney, intestines, and pyloric caecum are the major target tissues. Infected intestines and pyloric caecum showed enlarged, multinucleated cells with intracytoplasmic inclusions as the target cells of MRV.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Yuting Fu, Yong Li, Wenfeng Zhang, Weixuan Fu, Wangdong Li, Zhiming Zhu, Shaoping Weng, Jianguo He, Chuanfu Dong
Summary: A bivalent inactivated vaccine against ISKNV/RSIV and SDDV was developed and evaluated in Asian seabass. The survival rates of immunized fish were between 86.67% and 96.7%. The study also provided important information on the clinical symptoms and pathological changes caused by the viruses, contributing to the prevention and control of megalocytiviruses in Asian seabass.
Article
Veterinary Sciences
Yuan Gao, Nicolas M. Suarez, Gavin S. Wilkie, Chuanfu Dong, Sven Bergmann, Pei-Yu Alison Lee, Andrew J. Davison, Alain F. C. Vanderplasschen, Maxime Boutier
VETERINARY RESEARCH
(2018)