Article
Cell Biology
Anna Frappaolo, Roberto Piergentili, Maria Grazia Giansanti
Summary: Drosophila dividing spermatocytes provide a suitable cell system to study the reorganization of microtubule and actin cytoskeleton systems during cell division. The unique characteristics of Drosophila male meiotic cells make them amenable to investigate the molecular pathways regulating spindle microtubules and cytokinesis, with important implications for cancer and other diseases.
Article
Biology
Aastha Kumari, Avishek Ghosh, Sourav Kolay, Padinjat Raghu
Summary: This study reveals the mechanism by which PIP5K activity is coupled to PLC-mediated PI(4,5)P-2 hydrolysis through the identification of a unique isoform called dPIP5K(L) and the involvement of septin.
LIFE SCIENCE ALLIANCE
(2022)
Article
Multidisciplinary Sciences
Jia-Yi Wei, Sao-Yu Chu, Yu-Chien Huang, Pei-Chi Chung, Hung-Hsiang Yu
Summary: Neurogenesis in the Drosophila central brain is influenced by a novel mutation called Nuwa, with significant impairment occurring during early larval stages. The mutation affects the generation of larval-born olfactory projection neurons but not embryonic-born neurons. Molecular characterization reveals that the Nuwa mutation is a loss-of-function mutation in the Drosophila septin interacting protein 1 (sip1). SIP1 expression is enriched in neuroblasts, and its knockdown leads to abnormal brain formation. The full-length SIP1 protein is necessary for rescuing the neurogenesis defect caused by the Nuwa mutation.
SCIENTIFIC REPORTS
(2022)
Article
Biology
Bharath Sunchu, Nicole MynYi Lee, Jennifer A. Taylor, Roberto Carlos Segura, Chantal Roubinet, Clemens Cabernard
Summary: In this study, a hybrid fly cell model was used to investigate the recognition and segregation of chromosomes of different origins. The findings suggest that alignment and separation of endogenous and ectopic chromatin in neural stem cell and ganglion mother cell hybrid cells is facilitated by centrosomes and spindles, as well as microtubule-dependent chromatin retention and nuclear envelope boundaries.
COMMUNICATIONS BIOLOGY
(2022)
Article
Physics, Multidisciplinary
Mainak Chatterjee, Arkya Chatterjee, Amitabha Nandi, Anirban Sain
Summary: Contraction of the cytokinetic ring during cell division leads to physical partitioning of a eukaryotic cell into two daughter cells. This process involves flows of actin filaments and myosin motors in the growing membrane interface at the midplane of the dividing cell. The arrangement of actomyosin filaments and the resulting active stresses influence the flow, and the contraction process of the ring exhibits both stable and unstable modes.
PHYSICAL REVIEW LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Anand Bakshi, Fabio Echegaray Iturra, Andrew Alamban, Miquel Rosas-Salvans, Sophie Dumont, Mustafa G. Aydogan
Summary: Cytoplasmic divisions in Drosophila embryos can occur independently of nuclear divisions and mitotic signals. These divisions can occur without the presence of centrosomes, but become essential in the absence of CDK/cyclin activity. Additionally, autonomous cytoplasmic divisions may help remove stalled nuclei during embryogenesis. The mechanism governing the periodicity of cytoplasmic divisions may be independent of CDK/cyclin complexes.
Article
Oncology
Guang Yang, Shota Hiruma, Akira Kitamura, Masataka Kinjo, Mithilesh Mishra, Ryota Uehara
Summary: The study reveals the molecular basis of exchangeability between ezrin and other actin-membrane associated proteins during cytokinesis, showing specific designs of actin- or membrane-associated moieties with limited exchangeability. Depletion of anillin and supervillin leads to the overaccumulation of different domains of ezrin at the cleavage furrow, suggesting differential binding sites of ezrin with these proteins. Chimeric mutants demonstrate that ezrin's C-terminal domain can substitute for anillin domains in cytokinesis and cell proliferation, while anillin domains cannot substitute for ezrin domains in controlling cortical activities.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Sathish Thiyagarajan, Shuyuan Wang, Ting Gang Chew, Junqi Huang, Lokesh Kumar, Mohan K. Balasubramanian, Ben O'Shaughnessy
Summary: This study observed the full course of instability in isolated cytokinetic contractile rings, revealing that the turnover time of myosin II controls actomyosin contractile instability in normal cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Cell Biology
Giulia Russo, Michael Krauss
Summary: Cytokinesis involves the final separation of a mother cell into two daughter cells, with septins playing a crucial role in this process. Septins are recruited to the cleavage furrow early on and undergo constant rearrangement during cytokinetic progression, ultimately concentrating within the intercellular bridge. This concentration of septins at specific sites may support cytokinetic progression by interacting with actin, microtubules, and associated motors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Takahiro Yamamoto, Ryota Uehara
Summary: Tetraploidization in human cells leads to severe cell shape instability during cytokinesis, accompanied by ectopic contractile activity of the cell cortex. Over-accumulation of myosin II at the cell poles is found to be the primary cause of the cell shape instability in tetraploid cells. The suppression of myosin II activity restores smooth cell shape during cytokinesis, providing insights into the molecular mechanisms of tetraploidy-driven pathogenic processes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Medicine, Research & Experimental
Matheus Felipe da Silva, Luan Vitor Alves de Lima, Liana Martins de Oliveira, Simone Cristine Semprebon, Nayane de Oliveira Silva, Amanda Passuello de Aguiar, Mario Sergio Mantovani
Summary: This study aimed to investigate the antiproliferative activity of Diosgenin (DG) in NCI-H460 lung carcinoma cells and explore the underlying mechanisms of antimitotic and alternative cell death pathways. The results showed that DG reduced cell viability and concentration, increased the frequency of cells with multiple nuclei, and modulated gene expression related to cell cycle and cell death. Furthermore, DG also exhibited inhibitory effects on proliferation recovery and clonogenic growth in tumor spheroids.
Article
Multidisciplinary Sciences
Gerard Terradas, Jared B. Bennett, Zhiqian Li, John M. Marshall, Ethan Bier
Summary: CRISPR-based gene drives can be composed of a single cassette or separate elements, offering different control options. In this study, the authors compare the performance of genetically transformed full and split drives, and find that the full drive initially exhibits slower kinetics but eventually catches up to the split drive. These unexpected kinetics are likely due to transient fitness costs associated with individuals inheriting Cas9 and gRNA transgenes during the drive process.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Martin R. Gill, Paul J. Jarman, Vanessa Hearnden, Simon D. Fairbanks, Marcella Bassetto, John Palmer, Kathryn R. Ayscough, Jim A. Thomas, Carl Smythe
Summary: RuRuPhen blocks the transformation of G-actin monomers to F-actin filaments and disrupts the organization of actin stress fibers in cells. It interferes with actomyosin contractility, cell motility, and late-stage cytokinesis. RuRuPhen also interferes with the recruitment of endosomal sorting complexes required for transport (ESCRT) complex, leading to cytokinetic abscission failure.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Multidisciplinary Sciences
Kyung-Tae Park, Sebastien Pichoff, Shishen Du, Joe Lutkenhaus
Summary: Our study demonstrates that the overexpression of an activated FtsA (FtsA*) can compensate for the recruitment function of FtsQ, rescue FtsL and FtsB mutants deficient in FtsW recruitment, and also rescue an FtsL mutant unable to deliver the periplasmic signal from FtsN, suggesting that FtsA* acts on FtsW. Additionally, we found that an FtsW mutant specifically defective in activation by FtsA was rescued by an activated FtsQLB, indicating a synergistic relationship between active FtsA and active FtsQLB in activating FtsWI for sPG synthesis in response to FtsN.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Editorial Material
Biochemistry & Molecular Biology
Tyson L. Hedrick, Bradley H. Dickerson
Summary: A new study using neurogenetic methods and a virtual reality flight arena has uncovered a group of descending neurons in Drosophila that fully activate flight motor and steer the fly by independently regulating the left and right wings.
Article
Cell Biology
Jieyan V. Chen, Ling-Rong Kao, Swadhin C. Jana, Elena Sivan-Loukianova, Susana Mendonca, Oscar A. Cabrera, Priyanka Singh, Clemens Cabernard, Daniel F. Eberl, Monica Bettencourt-Dias, Timothy L. Megraw
JOURNAL OF CELL BIOLOGY
(2015)
Article
Multidisciplinary Sciences
Michaela Roth, Chantal Roubinet, Niklas Ifflaender, Alexia Ferrand, Clemens Cabernard
NATURE COMMUNICATIONS
(2015)
Article
Cell Biology
Anjana Ramdas Nair, Priyanka Singh, David Salvador Garcia, David Rodriguez-Crespo, Boris Egger, Clemens Cabernard
Article
Multidisciplinary Sciences
Emilie Montembault, Marie-Charlotte Claverie, Lou Bouit, Cedric Landmann, James Jenkins, Anna Tsankova, Clemens Cabernard, Anne Royou
NATURE COMMUNICATIONS
(2017)
Article
Multidisciplinary Sciences
Chantal Roubinet, Anna Tsankova, Tri Thanh Pham, Arnaud Monnard', Emmanuel Caussinus, Markus Affolter, Clemens Cabernard
NATURE COMMUNICATIONS
(2017)
Editorial Material
Biology
Clemens Cabernard
Article
Biochemistry & Molecular Biology
Priyanka Singh, Anjana Ramdas Nair, Clemens Cabernard
Article
Cell Biology
Tiantian Ji, Lina Zhan, Mingxi Deng, Shengshuo Huang, Ying Wang, Tri Thanh Pham, Andrew Alan Smith, Varun Sridhar, Clemens Cabernard, Jiguang Wang, Yan Yan
DISEASE MODELS & MECHANISMS
(2019)
Review
Developmental Biology
Bharath Sunchu, Clemens Cabernard
Article
Biochemistry & Molecular Biology
Emmanuel Gallaud, Anjana Ramdas Nair, Nicole Horsley, Arnaud Monnard, Priyanka Singh, Tri Thanh Pham, David Salvador Garcia, Alexia Ferrand, Clemens Cabernard
Review
Cell Biology
Melissa K. Delgado, Clemens Cabernard
CURRENT OPINION IN CELL BIOLOGY
(2020)
Article
Cell Biology
Katarzyna Lepeta, Chantal Roubinet, Milena Bauer, M. Alessandra Vigano, Gustavo Aguilar, Oguz Kanca, Amanda Ochoa-Espinosa, Dimitri Bieli, Clemens Cabernard, Emmanuel Caussinus, Markus Affolter
Summary: Reversible protein phosphorylation by kinases is crucial for the development and homeostasis of multicellular organisms. However, studying defined kinase-substrate interactions is challenging due to complex signaling networks and multiple phosphorylation targets. To overcome this, researchers have created protein binder-based engineered kinases that can directly and specifically phosphorylate fluorescent fusion proteins in vivo, allowing for targeted phosphorylation of chosen targets.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biology
Bharath Sunchu, Nicole MynYi Lee, Jennifer A. Taylor, Roberto Carlos Segura, Chantal Roubinet, Clemens Cabernard
Summary: In this study, a hybrid fly cell model was used to investigate the recognition and segregation of chromosomes of different origins. The findings suggest that alignment and separation of endogenous and ectopic chromatin in neural stem cell and ganglion mother cell hybrid cells is facilitated by centrosomes and spindles, as well as microtubule-dependent chromatin retention and nuclear envelope boundaries.
COMMUNICATIONS BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Tri Thanh Pham, Arnaud Monnard, Jonne Helenius, Erik Lund, Nicole Lee, Daniel J. Muller, Clemens Cabernard
Article
Cell Biology
Anna Tsankova, Tri Thanh Pham, David Salvador Garcia, Fabian Otte, Clemens Cabernard
DEVELOPMENTAL CELL
(2017)