Article
Biochemistry & Molecular Biology
Diane Frankel, Isabelle Nanni, L'Houcine Ouafik, Clara Camilla, Eric Pellegrino, Nathalie Beaufils, Laurent Greillier, Herve Dutau, Philippe Astoul, Elise Kaspi, Patrice Roll
Summary: ALK and ROS1 rearrangement detection in advanced-stage non-small cell lung cancer can be performed using multiple techniques, including ICC, FISH, and NGS. Compared to NGS, ICC has lower sensitivity and specificity, while FISH has similar sensitivity and specificity. FISH can correct false-positive cases obtained by ICC. Although NGS has better detection capability for ALK and ROS1 rearrangements in cytological samples, it is still much more expensive than the sequential use of ICC and FISH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Clinical Neurology
Jes Olesen
Summary: This article reviews the organization of neurology at the European level, highlighting the involvement of all European national neurological societies and the World Federation of Neurology in the formation of the European Federation of Neurological Societies (EFNS). The EFNS successfully developed organizational structures, scientific committees, educational courses, and held successful congresses, ultimately leading to the creation of the European Academy of Neurology (EAN).
EUROPEAN JOURNAL OF NEUROLOGY
(2023)
Article
Veterinary Sciences
Yu Furusawa, Mariko Shima-Sawa, Tatsuro Hifumi, Noriaki Miyoshi, Osamu Yamato, Akira Yabuki
Summary: In this study, the usefulness of cryopreserved air-dried smears for immunocytochemical staining was evaluated. The results showed that immunoreactivity for cytokeratin and vimentin could be detected for at least 33 months in the cryopreserved smears, demonstrating the usefulness and effectiveness of this method in veterinary immunocytochemistry.
VETERINARY SCIENCES
(2023)
Article
Cell Biology
Peyman Dinarvand, Wei-Lien Wang, Sinchita Roy-Chowdhuri
Summary: SOX11 immunocytochemistry can serve as a useful diagnostic marker for SPN, in addition to beta-catenin, and can be performed on cytological smears in cases without a cell block preparation.
Article
Clinical Neurology
Ettore Beghi, Heinrich Binder, Codruta Birle, Natan Bornstein, Karin Diserens, Stanislav Groppa, Volker Homberg, Vitalie Lisnic, Maura Pugliatti, Gary Randall, Leopold Saltuari, Stefan Strilciuc, Johannes Vester, Dafin Muresanu
Summary: This study reviews the impact of early pharmacological support on neurorehabilitation after stroke, recommending certain interventions and advising against others for clinical use. The findings aim to assist healthcare professionals in making informed decisions for stroke survivors' recovery.
EUROPEAN JOURNAL OF NEUROLOGY
(2021)
Review
Pathology
Veronika Anic, Maj Liv Eide, Beatrix Cochand-Priollet, Danijela Vrdoljak Mozetic, Giovanni Negri, Philippe Vielh
Summary: The recommended education program, developed by EACC and EFCS, aims to provide optimal education and training for future cytotechnology professionals.
Article
Clinical Neurology
Mario Habek, Fabian Leys, Magdalena Krbot Skoric, Diogo Reis Carneiro, Giovanna Calandra-Buonaura, Jennifer Camaradou, Giacomo Chiaro, Pietro Cortelli, Cristian Falup-Pecurariu, Roberta Granata, Pietro Guaraldi, Raimund Helbok, Max J. Hilz, Valeria Iodice, Jens Jordan, Evert C. A. Kaal, Anita Kamondi, Anne Pavy Le Traon, Isabel Rocha, Johann Sellner, Jean Michel Senard, Astrid Terkelsen, Gregor K. Wenning, Thomas Berger, Roland D. Thijs, Walter Struhal, Alessandra Fanciulli
Summary: This study investigated neurology-driven or interdisciplinary clinical laboratories for autonomic nervous system disorders in Europe and found disparities in healthcare services availability among countries. There is a need for improved access to specialized care, particularly in South, East, and Greater Europe.
EUROPEAN JOURNAL OF NEUROLOGY
(2022)
Article
Oncology
Masami Kawaharada, Manabu Yamazaki, Satoshi Maruyama, Tatsuya Abe, Nyein Nyein Chan, Taiichi Kitano, Tadaharu Kobayashi, Takeyasu Maeda, Jun-Ichi Tanuma
Summary: This study aimed to establish a novel experimental model to observe changes in mRNA and protein expression patterns in a rat model of tongue cancer. The results showed that the mRNA expression levels of Brd4, c-Myc, and Tp53 were upregulated during the progression from negative to squamous cell carcinoma. This experimental model allowed the observation of sequential morphological changes and the expression patterns of mRNAs and proteins during carcinogenesis, and combining immunocytochemistry with cytology-based diagnoses may improve the diagnostic accuracy of OSCC.
Article
Acoustics
Fernando Alfageme, Ximena Wortsman, Orlando Catalano, Gaston Roustan, Maria Crisan, Diana Crisan, Diana E. Gaitini, Eugenio Cerezo, Radu Badea
Summary: Although the scientific literature concerning dermatologic ultrasound is still insufficient for evidence-based guidelines, general recommendations from scientific societies are deemed necessary. The EFSUMB steering committee has developed a series of consensus position statements on various aspects of dermatologic ultrasound, laying the foundation for future evidence-based recommendations and guidelines.
ULTRASCHALL IN DER MEDIZIN
(2021)
Article
Clinical Neurology
Alessandra Fanciulli, Fabian Leys, Magdalena Krbot Skoric, Diogo Reis Carneiro, Giovanna Calandra-Buonaura, Jennifer Camaradou, Giacomo Chiaro, Pietro Cortelli, Cristian Falup-Pecurariu, Roberta Granata, Pietro Guaraldi, Raimund J. Helbok, Max Hilz, Valeria Iodice, Jens Jordan, Evert C. A. Kaal, Anita Kamondi, Anne Pavy Le Traon, Isabel Rocha, Johann Sellner, Jean Michel Senard, Astrid K. Terkelsen, Gregor Wenning, Elena Moro, Thomas D. Berger, Roland Thijs, Walter Struhal, Mario Habek
Summary: This study investigated the impact of the COVID-19 pandemic on European clinical autonomic practice. The results showed a significant decrease in the number of autonomic tests and visits during the first year of the pandemic. COVID-19 infection was associated with the development or worsening of cardiovascular autonomic disorders, while the association with COVID-19 vaccines was deemed unlikely.
EUROPEAN JOURNAL OF NEUROLOGY
(2023)
Article
Cell Biology
Jan Philipp Kuehn, Stefanie Speicher, Barbara Linxweiler, Sandrina Koerner, Hugo Rimbach, Mathias Wagner, Erich Franz Solomayer, Bernhard Schick, Maximilian Linxweiler
Summary: Dual immunocytochemical detection of Sec62 and Ki67 using liquid-based swab cytology can enable non-invasive early detection of head and neck squamous cell carcinomas with high sensitivity and specificity.
Review
Pharmacology & Pharmacy
Ancuta Cristina Raclariu-Manolica, Quentin Mauvisseau, Hugo J. de Boer
Summary: Although herbal medicines and preparations are widely used in healthcare systems globally, there are concerns about their quality and safety. The introduction of new herbal products under varying regulatory frameworks, with no global consensus on their definition or characterization, creates concerns about contamination and profit-driven adulteration. Collaboration between industry, academia, and regulatory bodies is necessary to develop innovative strategies for identification and authentication of botanicals and their preparations to ensure quality control.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Chemistry, Analytical
Guangzheng Xu, Hui Wang, Yingqian Deng, Keyi Xie, Weibo Zhao, Xingchu Gong
Summary: The review compared the formulations and quality standards of Xiaochaihu preparations at home and abroad, discussed the differences in manufacturing processes, and reviewed the progress of research on qualitative identification, quantitative detection, and fingerprint chromatograms. It also suggested improvements in drug quality detection methods and setting limits for specific components in order to enhance drug safety and efficacy.
Article
Medical Laboratory Technology
Sarah E. E. Wheeler, Ivan M. M. Blasutig, Pradeep Kumar Dabla, Jean-Marc Giannoli, Anne Vassault, Ji Lin, Kandace A. A. Cendejas, Armand Perret-Liaudet, Renze Bais, Annette Thomas, Egon P. P. Amann, Qing H. H. Meng
Summary: According to a survey conducted by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC), there is significant variation in internal quality control (IQC) practices and management worldwide. While 78.3% of countries have regulations or accreditation requirements for medical laboratory quality standards, they are not mandatory in 46.7% of these countries. This highlights the need for standardized programs and education to improve overall quality management in medical laboratories.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Pathology
Hamza Tariq, Paolo Gattuso, Lin Cheng
Summary: Myeloid sarcoma is a rare malignancy with clinical, morphologic, immunophenotypic, and genetic heterogeneity. This study highlights the morphologic features of myeloid sarcoma and investigates the efficacy of pathologist-performed rapid on-site evaluation in ensuring accurate triage.
Review
Medical Laboratory Technology
Ashish Chandra, Fernando Schmitt
Summary: Diagnostic uncertainty is common in cytological evaluation, with the quality and quantity of the sample playing a key role. In serous fluid cytopathology, there has historically been a lack of defined criteria for atypia and suspicious categories, leading to interchangeable usage. The international system aims to provide clear scenarios for using these terms and propose diagnostic criteria and management strategies.
DIAGNOSTIC CYTOPATHOLOGY
(2022)
Review
Pathology
Kemal Behzatoglu, Fernando Schmitt
Summary: Small cell tumors in the breast, including neuroendocrine neoplasms and lymphomas, present a diverse range of cytological features and should be carefully evaluated with consideration of clinical features. Due to the wide variety of tumors with small cell characteristics in the breast, they should be categorized into neuroendocrine and non-neuroendocrine tumors for proper diagnosis.
Article
Medicine, General & Internal
Sule Canberk, Helena Barroca, Ines Girao, Ozlem Aydin, Aysun Uguz, Kivilcim Erdogan, Ebru Tastekin, Massimo Bongiovanni, Paula Soares, Valdemar Maximo, Fernando Schmitt
Summary: This study evaluated the performance of TBSRTC in the paediatric population and questioned the management recommendation of ATA Guidelines Task Force on Paediatric Thyroid Cancer. The results showed high risk of malignancy in the paediatric population and a high rate of malignancy in operated patients, indicating a need for better preoperative indicators for stratification.
Review
Cell Biology
Daniel Pinto, Fernando C. Schmitt
Summary: Fine-needle aspiration biopsies (FNABs) of the breast are minimally invasive procedures that can diagnose suspicious breast lesions. When handled properly, FNAB samples can provide sufficient material for ancillary testing. Immunocytochemistry (ICC) can differentiate between different types of cancer, and cellblocks are more versatile than cytology slides. Laboratories without experience should validate these techniques before adopting them.
Article
Oncology
Sara Ricardo, Pedro Canao, Diana Martins, Ana C. Magalhaes, Marina Pereira, Ulysses Ribeiro-Junior, Evandro Sobroza de Mello, Venancio A. Alves, Regina Pinto, Dina Leitao, Georgina Alves, Rute Oliveira, Joana Wilton, Susete Costelha, Diana Meireles, Didier Cabanes, Leonor David, Fernando Schmitt
Summary: This study evaluated the expression of ACE2 and furin in cancer cells and found that colon and gastric carcinoma cells have high expression levels of ACE2 and furin, while thyroid carcinoma cells do not express ACE2. Although some cases tested positive for SARS-CoV-2 by tissue section PCR, immunohistochemistry and in situ hybridization did not show viral presence in the cancer cells. This study raises the possibility of ACE2-mediated viral tropism in cancer tissues to be clarified in future studies.
Review
Cell Biology
Emad A. Rakha, Ewa Chmielik, Fernando C. Schmitt, Puay Hoon Tan, Cecily M. Quinn, Grace Gallagy
Summary: The management of breast cancer patients relies on the assessment of prognostic and predictive markers. This review provides an update on the assessment of key molecular markers in breast cancer, including hormone receptors, HER2, and KI67, and highlights some challenges and focus areas.
Article
Medical Laboratory Technology
Fernando Schmitt, Maria D. Lozano
Summary: The understanding of molecular mechanisms in lung cancer and the discovery of targetable genomic alterations have significantly changed the pathological approach to lung cancer, particularly non-small cell lung cancer (NSCLC). Pathologists have been at the forefront of lung cancer management due to this unstoppable knowledge. In the field of cytopathology, where maximizing output with minimal input is a daily challenge, there has been a paradigm shift in the care and handling of diagnostic samples. This shift emphasizes the utilization of cytologic samples and small biopsies for both diagnostic and ancillary testing purposes.
DIAGNOSTIC CYTOPATHOLOGY
(2023)
Article
Pathology
Fernando C. Schmitt, Lukas Bubendorf, Sule Canberk, Ashish Chandra, Ian A. Cree, Marianne Engels, Kenzo Hiroshima, Deepali Jain, Ivana Kholova, Lester Layfield, Ravi Mehrotra, Claire W. Michael, Robert Osamura, Martha B. Pitman, Sinchita Roy-Chowdhuri, Yukitoshi Satoh, Paul VanderLaan, Maureen F. Zakowski, Andrew S. Field
Summary: The International Academy of Cytology and the International Agency for Research on Cancer have collaborated to develop a WHO Reporting System for Lung Cytopathology. This system defines five categories for reporting lung cytopathology and provides a standardized approach for testing and diagnosing lung diseases worldwide. It aims to improve patient care and raise the profile of cytopathology.
Article
Pathology
Paula de Melo Alves, Fernando Ferreira, Telma Oliveira, Daniela Alves, Sule Canberk, Fernando C. Schmitt
Summary: Thyroid fine-needle aspiration (FNA) is a well-established technique for primary diagnosis. This study compared the staining effects of two methods, namely Diff-Quik (DQ) and BlueStain (R), and found that BlueStain (R) showed better preservation of colloid and cell details, making it a more cost-effective and simpler alternative to DQ stain.
Editorial Material
Medical Laboratory Technology
Fernando Schmitt, Zhongbo Yang, Magda Esebua
Summary: In the era of personalized medicine, molecular testing plays a critical role in patient care, particularly in cytology samples. It increases diagnostic sensitivity and specificity and helps select appropriate treatment for patients. This special issue provides readers with more information about molecular cytology through a review of literature and discussions on the basics of molecular techniques.
DIAGNOSTIC CYTOPATHOLOGY
(2023)
Article
Cell Biology
Christopher J. VandenBussche, Barbara Crothers, Ashish Chandra, Fernando Schmitt, Daniel F. I. Kurtycz
Summary: Objective: An international panel conducted a survey to identify opinions and explore existing practice patterns regarding body fluid cytopathology. The survey collected 593 international participant responses, revealing strong support for the development of a unified system for reporting body fluid cytopathology among respondents.
Article
Cell Biology
Gisela Magalhaes, Rita Calisto, Catarina Freire, Regina Silva, Diana Montezuma, Sule Canberk, Fernando Schmitt
Summary: Digital pathology is the future for histopathology laboratories, but the implementation process requires workflow reorganisation and often overlooks the role of Histotechnologists (HTLs). This article aims to provide guidance and practical recommendations for HTLs to contribute to the implementation of a new digital workflow, as well as to improve study programs and education regarding HTLs in digital pathology.
JOURNAL OF HISTOTECHNOLOGY
(2023)
Article
Oncology
Carla Luis, Fernando Schmitt, Rute Fernandes, Nuno Coimbra, Joana Rigor, Paula Dias, Dina Leitao, Ruben Fernandes, Raquel Soares
Summary: This study investigated the expression of enzymes involved in glycolysis and gluconeogenesis in breast cancer, and found significant differences based on molecular subtype and body mass index. These differences were closely related to hormonal receptor and HER2 status, as well as pathological stage and histological grade. Obesity appeared to attenuate these differences, but adipocyte deposition and inflammatory infiltration did not affect the differences. Additionally, obesity-related conditions such as diabetes, hypertension, and dyslipidemia influenced the expression of certain enzymes.
Article
Oncology
Ines Brandao Rego, Sara Coelho, Patricia Miguel Semedo, Joana Cavaco-Silva, Laetitia Teixeira, Susana Sousa, Joana Reis, Rui Dinis, Fernando Schmitt, Noemia Afonso, Jose Luis Fougo, Francisco Pavao, Ricardo Baptista Leite, Luis Costa
Summary: This study aims to describe the compliance with EUSOMA indicators in breast cancer management in different hospital settings in Portugal. Although most indicators meet EUSOMA standards, there are differences among institutions, especially in diagnosis and disease stages.
Article
Endocrinology & Metabolism
Cosimo Durante, Laszlo Hegedues, Agnieszka Czarniecka, Ralf Paschke, Gilles Russ, Fernando Schmitt, Paula Soares, Tamas Solymosi, Enrico Papini
Summary: With the widespread use of imaging techniques, an increasing number of thyroid nodules are discovered. Most are benign and do not require treatment, while small intrathyroidal neoplasms can be safely managed conservatively. Guidelines are needed to provide a cost-effective and patient-centered approach to managing this prevalent condition. The present guidelines aim to provide a clinical practice guide for the initial workup and management of thyroid nodules in adults, and are not intended for the management of thyroid malignancy.
EUROPEAN THYROID JOURNAL
(2023)
