Journal
CYTOMETRY PART A
Volume 87A, Issue 6, Pages 541-548Publisher
WILEY-BLACKWELL
DOI: 10.1002/cyto.a.22609
Keywords
high-content; HCA; screening; imaging; microfluidic; high-throughput; live-cell assay; receptor capping
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Funding
- National Institutes of Health [R01 HG00189]
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A parallel microfluidic cytometer (PMC) is based on a one-dimensional (1D) scanning detector, a parallel array of flow channels, and new multiparameter analysis algorithms that operate on low-pixel-count 1D images. In this article, we explore a series of image-based live- and fixed-cell screening assays, including two NF-kB nuclear translocations and T-cell capping. We then develop a new multiparametric linear weighted classifier that achieves a Z factor sufficient for scaled pharmaceutical discovery with Jurkat cells in suspension. We conclude that the PMC should have the throughput and statistical power to permit a new capability for image-based high-sample-number pharmaceutical screening with suspension samples. (c) 2014 International Society for Advancement of Cytometry
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