Förster resonance energy transfer microscopy and spectroscopy for localizing protein-protein interactions in living cells

FRET microscopy in the living cell: Different approaches, strengths and weaknesses

(2012) Sergi Padilla-Parra et al.   BIOESSAYS

Spatial Regulation of RhoA Activity during Pancreatic Cancer Cell Invasion Driven by Mutant p53

(2011) P. Timpson et al.   CANCER RESEARCH

FLIM FRET Technology for Drug Discovery: Automated Multiwell-Plate High-Content Analysis, Multiplexed Readouts and Application in Situ

(2011) Sunil Kumar et al.   CHEMPHYSCHEM

Quantification of protein interaction in living cells by two-photon spectral imaging with fluorescent protein fluorescence resonance energy transfer pair devoid of acceptor bleed-through

(2011) Jiho Kim et al.   CYTOMETRY PART A

Spectral resolution in conjunction with polar plots improves the accuracy and reliability of FLIM measurements and estimates of FRET efficiency

(2011) Y.-C. CHEN et al.   JOURNAL OF MICROSCOPY

Fluorescence Resonance Energy Transfer Assay for High-Throughput Screening of ADAMTS1 Inhibitors

(2011) Jianhao Peng et al.   MOLECULES

Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy

(2011) Yuansheng Sun et al.   Nature Protocols

An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching

(2011) Michele L. Markwardt et al.   PLoS One

Apolipoprotein E: Isoform Specific Differences in Tertiary Structure and Interaction with Amyloid-β in Human Alzheimer Brain

(2011) Phillip B. Jones et al.   PLoS One

In vivo fluorescence lifetime tomography of a FRET probe expressed in mouse

(2011) James McGinty et al.   Biomedical Optics Express

Three-Color Spectral FRET Microscopy Localizes Three Interacting Proteins in Living Cells

(2010) Yuansheng Sun et al.   BIOPHYSICAL JOURNAL

FRET Microscopy in 2010: The Legacy of Theodor Förster on the 100th Anniversary of his Birth

(2010) Yuansheng Sun et al.   CHEMPHYSCHEM

Actin cytoskeleton-dependent Rab GTPase-regulated angiotensin type I receptor lysosomal degradation studied by fluorescence lifetime imaging microscopy

(2010) Hewang Li   JOURNAL OF BIOMEDICAL OPTICS

Additional correction for energy transfer efficiency calculation in filter-based Förster resonance energy transfer microscopy for more accurate results

(2010) Yuansheng Sun et al.   JOURNAL OF BIOMEDICAL OPTICS

FRET microscopy: from principle to routine technology in cell biology

(2010) A. PIETRASZEWSKA-BOGIEL et al.   JOURNAL OF MICROSCOPY

Spatially pathogenic forms of tau detected in Alzheimer's disease brain tissue by fluorescence lifetime-based Förster resonance energy transfer

(2010) Sergey Larionov et al.   JOURNAL OF NEUROSCIENCE METHODS

Podoplanin Associates with CD44 to Promote Directional Cell Migration

(2010) Ester Martín-Villar et al.   MOLECULAR BIOLOGY OF THE CELL

Bright cyan fluorescent protein variants identified by fluorescence lifetime screening

(2010) Joachim Goedhart et al.   NATURE METHODS

Fluorescent protein recruitment assay for demonstration and analysis ofin vivoprotein interactions in plant cells and its application toTobacco mosaic virusmovement protein

(2010) Emmanuel Boutant et al.   PLANT JOURNAL

A Flow Cytometry-Based FRET Assay to Identify and Analyse Protein-Protein Interactions in Living Cells

(2010) Carina Banning et al.   PLoS One

Cdc42 Interaction with N-WASP and Toca-1 Regulates Membrane Tubulation, Vesicle Formation and Vesicle Motility: Implications for Endocytosis

(2010) Wenyu Bu et al.   PLoS One

Quantitative Comparison of Different Fluorescent Protein Couples for Fast FRET-FLIM Acquisition

(2009) Sergi Padilla-Parra et al.   BIOPHYSICAL JOURNAL

Homo-FRET Imaging Enables Quantification of Protein Cluster Sizes with Subcellular Resolution

(2009) Arjen N. Bader et al.   BIOPHYSICAL JOURNAL

Practical and reliable FRET/FLIM pair of fluorescent proteins

(2009) Dmitry Shcherbo et al.   BMC BIOTECHNOLOGY

The fluorescent protein palette: tools for cellular imaging

(2009) Richard N. Day et al.   CHEMICAL SOCIETY REVIEWS

Evaluation of intensity-based ratiometric FRET in image cytometry-Approaches and a software solution

(2009) János Roszik et al.   CYTOMETRY PART A

Three-dimensional polar representation for multispectral fluorescence lifetime imaging microscopy

(2009) A. Leray et al.   CYTOMETRY PART A

Characterization of an orange acceptor fluorescent protein for sensitized spectral fluorescence resonance energy transfer microscopy using a white-light laser

(2009) Yuansheng Sun et al.   JOURNAL OF BIOMEDICAL OPTICS

Photobleaching-Based Quantitative Analysis of Fluorescence Resonance Energy Transfer inside Single Living Cell

(2009) Longxiang Wang et al.   JOURNAL OF FLUORESCENCE

Genetically encoded FRET sensors to monitor intracellular Zn2+ homeostasis

(2009) Jan L Vinkenborg et al.   NATURE METHODS

Photoconversion in orange and red fluorescent proteins

(2009) Gert-Jan Kremers et al.   NATURE METHODS

Determination of supramolecular structure and spatial distribution of protein complexes in living cells

(2009) Valerica Raicu et al.   Nature Photonics

Structural rearrangement of CaMKII  catalytic domains encodes activation

(2009) C. Thaler et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

AccPbFRET: An ImageJ plugin for semi-automatic, fully corrected analysis of acceptor photobleaching FRET images

(2008) János Roszik et al.   BMC BIOINFORMATICS

Fluorescent protein tools for studying protein dynamics in living cells: a review

(2008) Richard N. Day et al.   JOURNAL OF BIOMEDICAL OPTICS

Rab4 and Rab11 coordinately regulate the recycling of angiotensin II type I receptor as demonstrated by fluorescence resonance energy transfer microscopy

(2008) Hewang Li et al.   JOURNAL OF BIOMEDICAL OPTICS

Characterization of an improved donor fluorescent protein for Förster resonance energy transfer microscopy

(2008) Richard N. Day et al.   JOURNAL OF BIOMEDICAL OPTICS

High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis

(2008) Clifford B. Talbot et al.   Journal of Biophotonics

Rapid Frequency-Domain FLIM Spinning Disk Confocal Microscope: Lifetime Resolution, Image Improvement and Wavelet Analysis

(2008) Chittanon Buranachai et al.   JOURNAL OF FLUORESCENCE

A quantitative protocol for dynamic measurements of protein interactions by Forster resonance energy transfer-sensitized fluorescence emission

(2008) A.D Elder et al.   Journal of the Royal Society Interface

Novel lambda FRET spectral confocal microscopy imaging method

(2008) Diego Megías et al.   MICROSCOPY RESEARCH AND TECHNIQUE

A practical guide to single-molecule FRET

(2008) Rahul Roy et al.   NATURE METHODS

Quantitative FRET Analysis With the E0GFP-mCherry Fluorescent Protein Pair

(2008) Lorenzo Albertazzi et al.   PHOTOCHEMISTRY AND PHOTOBIOLOGY

Quantum dot-based resonance energy transfer and its growing application in biology

(2008) Igor L. Medintz et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

A Comparison of Donor-Acceptor Pairs for Genetically Encoded FRET Sensors: Application to the Epac cAMP Sensor as an Example

(2008) Gerard N. M. van der Krogt et al.   PLoS One

The Phasor Approach to Fluorescence Lifetime Imaging Analysis

(2007) Michelle A. Digman et al.   BIOPHYSICAL JOURNAL

A novel fluorescence lifetime imaging system that optimizes photon efficiency

(2007) Ryan A. Colyer et al.   MICROSCOPY RESEARCH AND TECHNIQUE