Article
Optics
Oumeng Zhang, Zijian Guo, Yuanyuan He, Tingting Wu, Michael D. Vahey, Matthew D. Lew
Summary: Single-molecule orientation-localization microscopy is a powerful tool for studying biochemical processes, but the limited photon budget poses challenges for high-dimensional imaging. A raMVR microscope has been developed with isotropic 3D spatial resolution and precision in measuring molecular orientations, enabling detailed imaging of molecular dynamics within biological and chemical systems.
Article
Multidisciplinary Sciences
Amani A. Hariri, Sharon S. Newman, Steven Tan, Dan Mamerow, Alexandra M. Adams, Nicolo Maganzini, Brian L. Zhong, Michael Eisenstein, Alexander R. Dunn, H. Tom Soh
Summary: One major challenge in enzyme-linked immunosorbent assays (ELISAs) is distinguishing true signal from non-specific binding. The authors developed a Single-Molecule Colocalization Assay (SiMCA) that eliminates this issue and allows for reproducible detection of picomolar protein concentrations.
NATURE COMMUNICATIONS
(2022)
Review
Chemistry, Multidisciplinary
Weijie Chi, Davin Tan, Qinglong Qiao, Zhaochao Xu, Xiaogang Liu
Summary: Single-molecule localization microscopy (SMLM) has been widely used in biology and chemistry. Fluorophores, as an essential component of SMLM, play a crucial role in obtaining super-resolution fluorescence images. Recent research on spontaneously blinking fluorophores simplifies experimental setups and extends the imaging duration of SMLM. This review provides an overview of the development of spontaneously blinking rhodamines from 2014 to 2023 and the key mechanistic aspects of intramolecular spirocyclization reactions, aiming to accelerate the advancement of super-resolution imaging technologies.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Immunology
Maylin Merino-Wong, Barbara A. Niemeyer, Dalia Alansary
Summary: The study shows that PMCA4 expression varies in different human CD4(+) T cell compartments, with modulation of PMCA4 leading to different increases in SOCE, suggesting an important role for PMCA4 in regulating compartment stoichiometry.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Analytical
Xiaoshuang He, Xianliang Xu, Yue Sui, Ke Xu, Sicen Wang
Summary: The study established an α(1A)/CMC method for screening active compounds from Tetradium ruticarpum fruits and analyzing the binding characteristics with the receptor. Dehydroevodiamine was identified as a potential active compound through this method.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Analytical
Rouba Nasreddine, Reine Nehme
Summary: Microscale thermophoresis (MST) is a sensitive biophysical tool that allows rapid and accurate determination of the dissociation constant (K-d) between enzymes and small molecules, aiding in the study of binding affinity between biomolecules. The study demonstrated that hyaluronidase maintained enzymatic activity after fluorescent labelling, but showed no affinity with flavonoid compounds under the tested conditions.
MICROCHEMICAL JOURNAL
(2021)
Article
Immunology
Miwa Sasai, Ji Su Ma, Masaaki Okamoto, Kohei Nishino, Hikaru Nagaoka, Eizo Takashima, Ariel Pradipta, Youngae Lee, Hidetaka Kosako, Pann-Ghill Suh, Masahiro Yamamoto
Summary: The TCR signaling in CD8(+) T cells is qualitatively different from that in CD4(+) T cells due to the involvement of PLC beta 4. PLC beta 4 plays a crucial role in activating CD8(+) T cells and promoting adaptive immunity, while deficiency of PLC beta 4 leads to impaired immune responses in CD8(+) T cells.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Chemistry, Physical
Huimin Cheng, Jun Yu, Zhen Wang, Ping Ma, Cunlan Guo, Bin Wang, Wenxuan Zhong, Bingqian Xu
Summary: Atomic force microscopy-single-molecule force spectroscopy is a powerful method for investigating molecular interactions in biological systems. To address the limitations of current algorithms, researchers have developed a network-based automatic clustering algorithm (NASA) for analyzing AFM-SMFS data and decoding specific molecular details.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Nanoscience & Nanotechnology
Abhinav Banerjee, Micky Anand, Simanta Kalita, Mahipal Ganji
Summary: DNA-PAINT super-resolution imaging is used to measure the free energy of dinucleotide base stacking at the single-molecule level. Results show that addition of a single dinucleotide base stacking can greatly stabilize the DNA duplex nanostructure. These findings are important for designing functional DNA nanostructures and predicting the local DNA structure.
NATURE NANOTECHNOLOGY
(2023)
Review
Chemistry, Analytical
Behrouz Seyfinejad, Sibel A. Ozkan, Abolghasem Jouyban
Summary: The article discusses the presence of drugs in the bloodstream in both free and bound forms, and how protein binding affects the free concentration of the drug. It highlights the importance of measuring the free concentration of drugs, their protein binding, and the benefits for personalized medicine and therapeutic drug monitoring.
Review
Biochemistry & Molecular Biology
Nitesh Kumar Podh, Sheetal Paliwal, Partha Dey, Ayan Das, Shruti Morjaria, Gunjan Mehta
Summary: Single-molecule imaging in live yeast cells has become a powerful tool for researchers to quantitatively measure cellular activities in real time, with significant progress made in recent years to provide important references for studying biological processes. Different labs have tailored imaging setups and data analysis pipelines to estimate biophysical parameters for various biological processes, reflecting a rich variety of experimental experiences.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ayoub Stelate, Eva Tihlarikova, Katerina Schwarzerova, Vilem Nedela, Jan Petrasek
Summary: A novel correlative light electron microscopy method has been developed for accurate study of domain organization of plant plasma membrane proteins, allowing quantification of the number of auxin efflux carriers within nanodomains.
Article
Biochemistry & Molecular Biology
Aleksandra Kotynia, Aleksandra Marciniak, Wojciech Kamysz, Damian Neubauer, Edward Krzyzak
Summary: In this project, the interaction between positively charged oligopeptides and crucial blood plasma proteins was studied using experimental characterization and molecular docking studies. The peptides investigated are rich in NH2 groups of amino acid side chains and have therapeutic properties. The binding interactions with blood plasma components, such as albumin, alpha 1-acid glycoprotein, and gamma-globulin fraction, were investigated under pseudo-physiological conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Yuqin Wang, Jiang Liu, Van Liem-Nguyen, Shanyi Tian, Siqi Zhang, Dingyong Wang, Tao Jiang
Summary: DOM influences the environmental fate and toxic effects of trace metals such as mercury. Different DOM isolates showed a wide range of binding strengths to Hg(II) due to their heterogeneous properties. DOM chromophores, including aromaticity and molecular weight, are critical indicators of the DOM-Hg affinity in ambient-relevant circumstances. Higher terrestrial DOM led to greater DOM-Hg affinity. UV-Vis and fluorescence spectroscopy can be used to estimate DOM composition and its binding strength with Hg(II). The relationship between logK and DOM properties provides a possible pathway of explanation for the spatial co-variations between Hg(II) concentrations and DOM characters observed in previous field investigations.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Diana B. Peckys, Daniel Gaa, Dalia Alansary, Barbara A. Niemeyer, Niels de Jonge
Summary: The study found that ORAI1 channels can form supra-molecular clusters, and different structures can be formed during SOCE activation, rather than simply being trapped near active STIM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Letter
Dermatology
Anna Gindl, Anna-Margarita Schotta, Sara Berent, Mateusz Markowicz, Hannes Stockinger, Florian Thalhammer, Georg Stary, Johanna Strobl
BRITISH JOURNAL OF DERMATOLOGY
(2022)
Biographical-Item
Immunology
Hannes Stockinger
IMMUNOLOGY LETTERS
(2022)
Article
Medicine, General & Internal
Anna-Margarita Schoetta, Theresa Stelzer, Gerold Stanek, Hannes Stockinger, Michiel Wijnveld
Summary: This study collected ticks in popular recreational areas in Vienna and found that the most prevalent pathogen was Borrelia burgdorferi sensu lato, which causes Lyme borreliosis in humans. Other pathogens such as Rickettsia, Babesia, Candidatus Neoehrlichia mikurensis (CNM), Anaplasma phagocytophilum, and Borrelia miyamotoi were also detected. This study identified hotspots for B. miyamotoi, which was detected in Vienna for the first time.
WIENER KLINISCHE WOCHENSCHRIFT
(2023)
Article
Immunology
Alexandra van der Staal, Janett Goehring, Anna Ohradanova-Repic, Markus Kramer, Clemens Donner, Andreas Zech, Marco Idzko, Hannes Stockinger
Summary: Interstitial lung disease poses challenges for diagnosis due to complex pathophysiological mechanisms, overlapping conditions, and interobserver disagreement. We developed a patient clustering model based on surface phenotyping of immune cells and clinical data to offer an additional approach in complex cases. Our model allows for rapid identification of divergent profiles within a diagnostic group and removes physician bias by relying on cellular nearest neighbors for sample clustering.
IMMUNOLOGY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Ingrid Hartl, Veronika Brumovska, Yasmin Striedner, Atena Yasari, Gerhard J. Schuetz, Eva Sevcsik, Irene Tiemann-Boege
Summary: This study utilized micropatterning to enrich FGFR3 in specific areas of the cell membrane, and quantified receptor activation by observing the recruitment of downstream signaling molecule GRB2 to FGFR3 micropatterns. The results showed that mutations in FGFR3 altered receptor activity, with increased activity in G380R and K650Q mutants upon ligand addition, while decreased activity in K650E mutant. These findings provide further insight into the functional effects of mutations to FGFR3.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biology
Astrid C. Erber, Angelika Wagner, Marianna Karachaliou, Maren Jeleff, Polyxeni Kalafatis, Manolis Kogevinas, Beata Peplonska, Isabel Santonja, Eva Schernhammer, Hannes Stockinger, Kurt Straif, Ursula Wiedermann, Thomas Waldhoer, Kyriaki Papantoniou
Summary: Data from a population-based study suggests that the time of day of COVID-19 vaccine administration has an impact on the level of antibodies produced. Morning vaccination was associated with the highest antibody levels, while midday vaccination was associated with the lowest levels. Further evaluation is needed to determine if this difference in antibody levels affects the level of protection against COVID-19.
JOURNAL OF BIOLOGICAL RHYTHMS
(2023)
Article
Biophysics
Clara Bodner, Dominik Kiesenhofer, Gerhard J. Schuetz, Mario Brameshuber
Summary: The interplay and communication between cells rely on protein-protein interactions and the oligomerization of proteins. The TOCCSL approach combines photobleaching and single-molecule microscopy to detect the interactions of fluorescently labeled membrane proteins even at high surface densities. Extensive Monte Carlo simulations were used to provide a theoretical framework for quantitative interpretation of TOCCSL measurements.
BIOPHYSICAL JOURNAL
(2023)
Biographical-Item
Immunology
Hannes Stockinger
IMMUNOLOGY LETTERS
(2023)
Article
Immunology
Peter A. Tauber, Bernhard Kratzer, Philipp Schatzlmaier, Ursula Smole, Cordula Koehler, Lisa Rausch, Jan Kranich, Doris Trapin, Alina Neunkirchner, Maja Zabel, Sabrina Jutz, Peter Steinberger, Gabriele Gadermaier, Thomas Brocker, Hannes Stockinger, Sophia Derdak, Winfried F. Pickl
Summary: This study used small molecule inhibitors to identify novel IL-2/Treg enhancing compounds. BX-795 was found to increase IL-2 production and inhibit Th2 cytokine secretion, inducing a Treg cell type similar to iTreg but lacking Foxp3 expression, and effectively treating allergic diseases by inhibiting Th2 inflammation.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Anna Ohradanova-Repic, Romana Prazenicova, Laura Gebetsberger, Tetiana Moskalets, Rostislav Skrabana, Ondrej Cehlar, Gabor Tajti, Hannes Stockinger, Vladimir Leksa
Summary: Lactoferrin is an iron-binding glycoprotein that is found in human exocrine fluids, especially breast milk. It has diverse roles in host defense, including modulating immune cell functions, sequestering iron, and binding to various surfaces. Lactoferrin can be cleaved to generate lactoferricin, which also has bioactive properties. This review discusses the structure, functions, and potential therapeutic uses of lactoferrin, lactoferricin, and other lactoferrin-derived peptides in treating infections and inflammatory conditions, with a focus on their potential use in treating COVID-19.
Article
Physics, Applied
Fabian Hinterer, Magdalena C. Schneider, Simon Hubmer, Montserrat Lopez-Martinez, Ronny Ramlau, Gerhard J. Schuetz
Summary: Single molecule localization microscopy relies on precise quantification of the positions of single dye emitters in a sample. The number of detected photons from each molecule determines the precision, which can be improved by recording at cryogenic temperatures to reduce photobleaching. However, long illuminations can cause image blur due to inevitable jitter or drift, which degrades localization precision. This paper demonstrates a method to largely eliminate drift effects by parallel recording fiducial marker beads and fitting the drift trajectory, applicable to drift magnitudes of several hundred nanometers per frame, and assuming fixed dipole orientations during each illumination.
APPLIED PHYSICS LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Rene Platzer, Joschka Hellmeier, Janett Gohring, Iago Doel Perez, Philipp Schatzlmaier, Clara Bodner, Margarete Focke-Tejkl, Gerhard J. Schuetz, Eva Sevcsik, Hannes Stockinger, Mario Brameshuber, Johannes B. Huppa
Summary: This study demonstrates that single freely diffusing agonist pMHC II can autonomously elicit a full T-cell response in a peptide-specific manner, while endogenous pMHC II has minimal impact on TCR engagement.
Article
Medicine, General & Internal
Erika Garner-Spitzer, Angelika Wagner, Venugopal Gudipati, Anna-Margarita Schoetta, Maria Orola-Taus, Michael Kundi, Renate Kunert, Patrick Mayrhofer, Johannes B. Huppa, Hannes Stockinger, Rita Carsetti, Pia Gattinger, Rudolf Valenta, Bernhard Kratzer, Al Nasar Ahmed Sehgal, Winfried F. Pickl, Walter Reinisch, Gottfried Novacek, Ursula Wiedermann
Summary: Patients with inflammatory bowel disease (IBD) receiving anti-TNF-alpha treatment showed lower and faster waning antibody levels after SARS-CoV-2 vaccination, likely due to impaired formation and maintenance of S-specific B memory cells. In contrast, IBD patients receiving alpha 4 beta 7-integrin antagonists and controls had higher and more persistent antibody levels, which correlated with the increased levels of S-specific B memory cells.
Article
Food Science & Technology
Nicole Ollinger, Alexandra Malachova, Michael Sulyok, Lisa Schutz-Kapl, Nicole Wiesinger, Rudolf Krska, Julian Weghuber
Summary: In this study, three reliable methods were used to identify mould species in food, aiming to estimate the safety of apparently non-moldy slices of bread and reduce food waste. The results showed that it might be safe to consume bread slices from lower layers of bread stacks, even if mould growth is visible on the outer slices.
Meeting Abstract
Dermatology
S. Mueller, A. Schoetta, V. Muendler, A. Gindl, L. Kleissl, A. Redl, L. Unterluggauer, D. Atzmueller, G. Stanek, R. Klasinc, M. Markowicz, H. Stockinger, J. Strobl, G. Stary
EXPERIMENTAL DERMATOLOGY
(2022)