Article
Biotechnology & Applied Microbiology
Abisha Crystal Christopher, Vigneshwaran Venkatesan, Karthik V. Karuppusamy, Saranya Srinivasan, Prathibha Babu, Manoj Kumar K. Azhagiri, Karthik Chambayil, Abhirup Bagchi, Vignesh Rajendiran, Nithin Sam Ravi, Sanjay Kumar, Srujan Kumar Marepally, Kumarasamypet Murugesan Mohankumar, Alok Srivastava, Shaji R. Velayudhan, Saravanabhavan Thangavel
Summary: In this study, a combination of small molecules was identified to selectively expand CD34(+)CD133(+)CD90(+) hematopoietic stem cells (HSCs) in ex vivo culture. The preferential expansion enriched the HSCs in culture and significantly increased long-term engraftment in NSG mice. The culture-enriched HSCs also showed a higher responsiveness to gene modification, resulting in a higher frequency of gene-modified HSCs. The yield of gene-modified HSCs obtained through culture enrichment was similar to sort-purification and superior to Cyclosporin-H treatment.
HUMAN GENE THERAPY
(2022)
Article
Endocrinology & Metabolism
Ye Cao, Scott M. Bolam, Anna L. Boss, Helen C. Murray, Jacob T. Munro, Raewyn C. Poulsen, Nicola Dalbeth, Anna E. S. Brooks, Brya G. Matthews
Summary: This study indicates considerable diversity in the non-hematopoietic cell populations within different tissue compartments of the adult human skeleton, and suggests that periosteal progenitor cells may reside within the CD90+CD34+ population.
Article
Immunology
Karthik V. Karuppusamy, John Paul Demosthenes, Vigneshwaran Venkatesan, Abisha Crystal Christopher, Prathibha Babu, Manojkumar K. Azhagiri, Annlin Jacob, Veena Vadhini Ramalingam, Sumathi Rangaraj, Mohankumar Kumarasamypet Murugesan, Srujan Kumar Marepally, George M. Varghese, Alok Srivastava, Rajesh Kannangai, Saravanabhavan Thangavel
Summary: This study demonstrates the potential of using CD34(+)CD90(+) hematopoietic stem cells (HSCs) and gene editing technology as an ideal graft for HIV gene therapy. Treatment with a small molecule cocktail improves the in vivo engraftment potential of CD34(+)CD90(+) HSCs, and gene editing of CCR5 generates immune cells resistant to HIV infection.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Mieun Lee-Theilen, Julia R. Hadhoud, Giulietta Volante, Delaine D. Fadini, Julia Eichhorn, Udo Rolle, Henning C. Fiegel
Summary: This study successfully identified CSCs of hepatoblastoma using CD34, CD90, OV-6, and csVimentin as markers. These CSCs exhibit characteristics of drug resistance and tumor relapse promotion.
Article
Multidisciplinary Sciences
Amrita Vats, Tzu-Chuan Ho, Irwin Puc, Chiung-Hsin Chang, Guey-Chuen Perng, Po-Lin Chen
Summary: In this study, a persistent infection model of dengue virus (DENV) in umbilical cord blood (UCB) was established, and CD133 and CD34 were identified as target cells for DENV infection. Recovering DENV particles from non-productive cells after co-culturing with Vero cells was observed. This research provides new insights into understanding the dynamics of DENV transmission and reactivation.
SCIENTIFIC REPORTS
(2023)
Article
Oncology
Xueping Li, Yuting Dai, Bing Chen, Jinyan Huang, Saijuan Chen, Lu Jiang
Summary: The study revealed that the expression levels of CD34(+)CD117(dim) and CD34(+)CD117(bri) myeloblast-associated genes impact clinical prognosis in patients with t(8;21) AML, providing new possibilities for identifying prognostic markers for this disease.
FRONTIERS OF MEDICINE
(2021)
Review
Oncology
Jianhui Yang, Omar Aljitawi, Peter Van Veldhuizen
Summary: This review summarizes the recent research progress on the role of CD133 in prostate cancer stem cells (PCSC), including its selective expression in undifferentiated cells, its correlation to treatment resistance, its gene regulation and functional analysis, and its targeted therapy in vitro, in vivo, and in clinical trials.
Article
Medicine, Research & Experimental
Fatemeh Sokouti Nasimi, Saber Zahri, Shahin Ahmadian, Afsaneh Bagherzadeh, Nahideh Nazdikbin Yamchi, Leila Haghighi, Alberto Miranda Bedate, Balal Khalilzadeh, Reza Rahbarghazi, Mahdi Mahdipour
Summary: The study showed that estradiol can increase the viability of mouse SSCs in a dose-dependent manner, leading to changes in cell morphology and gene expression, as well as reduced sperm parameters and testicular progenitor cells. This suggests that estradiol may induce Sertoli cell differentiation of SSCs while long-term exogenous administration can lead to infertility.
Article
Oncology
Yuanshuai Zhou, Xingjun Meng, Wen He, Xinying Li, Rongchuan Zhao, Caihua Dong, Detian Yuan, Jiao Yang, Ruobing Zhang, Guohua Shi, Yulun Huang, Jiangang Liu, Jianping Liu, Songbai Liu, Peng Fu, Minxuan Sun
Summary: This study reveals that the USF1/CD90 axis plays a crucial role in promoting malignant growth and stemness of glioblastoma stem cells (GSCs) through physical interaction with tumor-associated macrophages (TAMs). The findings suggest that targeting the USF1/CD90 axis may provide a potential therapeutic strategy for glioblastoma treatment.
Article
Biochemistry & Molecular Biology
Dong-Wook Min, Hwang-Phill Kim, Jinhyun Kim, Xianyu Wen, Sungsik Kim, Young-Won Cho, Yoojoo Lim, Sang-Hyun Song, Sae-Won Han, Sunghoon Kwon, Gyeong Hoon Kang, Tae-You Kim
Summary: Tumor heterogeneity in colon cancer therapy poses challenges, with single cell analysis revealing heterogeneous subclones within CD133 positive cancer stem cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Cynthia M. Simbulan-Rosenthal, Yogameenakshi Haribabu, Sahar Vakili, Li-Wei Kuo, Havens Clark, Ryan Dougherty, Ryyan Alobaidi, Bonnie Carney, Peter Sykora, Dean S. Rosenthal
Summary: This study investigates the underlying mechanisms of CD133's anti-apoptotic activity in malignant melanoma, revealing that CD133 activates a survival pathway and contributes to drug resistance. Targeting the CD133, AKT, or BCL-2 survival pathways shows potential for more effective treatments for high-risk melanoma patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pathology
Brittni M. Foster, Kendall L. Langsten, Ammar Mansour, Lihong Shi, Bethany A. Kerr
Summary: Stem cell factor (SCF) plays a crucial role in development, with continued expression in many cell types and tissues in adult humans and mice. High SCF expression in certain organs may be linked to an increased risk of SCF/CD117-related diseases.
EXPERIMENTAL AND MOLECULAR PATHOLOGY
(2021)
Article
Polymer Science
Rakshita Grover, Sahil Drall, Neelam Poonia, Gaurav Kumar Jain, Geeta Aggarwal, Viney Lather, Prashant Kesharwani, Deepti Pandita, Ramesh K. Goyal
Summary: Cancer stem cells (CSCs) contribute to tumor onset, progression, chemotherapy resistance, and relapse. CD44 and CD133 are commonly expressed markers used to identify aggressive CSCs. Targeting these markers with aptamers conjugated nanocarriers can overcome the limitations of antibodies and has proven to be beneficial in targeting CSCs. This article reviews the recent advancements in aptamers and their conjugation with nanocarriers for specific targeting of CSCs and improved cancer therapy efficacy.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Multidisciplinary Sciences
Xiong Shu, Weifeng Liu, Huiqi Liu, Hui Qi, Chengai Wu, Yu-Liang Ran
Summary: This study identified differential expression of miRNAs between CD133(+) and CD133(-) cells, with miR-4284 being a particularly interesting miRNA that may have significant roles in the function of OSCs, especially associated with the Wnt signaling pathway.
Article
Biotechnology & Applied Microbiology
Kurt Berckmueller, Justin Thomas, Eman A. Taha, Seunga Choo, Ravishankar Madhu, Greta Kanestrom, Peter B. Rupert, Roland Strong, Hans-Peter Kiem, Stefan Radtke
Summary: Novel HSC-targeted viral vectors have been developed that can specifically transduce CD90-expressing HSCs, and are expected to have significant potential in ex vivo gene therapy.
Article
Biochemistry & Molecular Biology
Nidhi Girdhar, Heena Kansal, Kranti Garg, Siddharth Sharma, K. Sandeep Prabhu, Vishal Chopra, Alexey A. Tinkov, Anatoly Skalny, N. Tejo Prakash
Summary: This study investigated the correlation between serum selenium levels and the severity of asthma. The results showed that asthma patients had lower serum selenium levels compared to healthy individuals, with no significant differences based on gender and age. Patients with a family history of asthma and those using inhalers had slightly lower selenium concentrations.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Article
Medicine, Research & Experimental
Sidhartha Singh, Navneet Singh, Manoj Baranwal, Siddharth Sharma
Summary: This study aims to examine the association between MSH2 polymorphisms and clinical outcomes in North Indian lung cancer patients.
BIOMARKERS IN MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Sidhartha Singh, Siddharth Sharma, Manoj Baranwal
Summary: This study aimed to identify single nucleotide polymorphisms (SNPs) of the MSH2 gene associated with cancer development using computational tools. Twenty-seven SNPs and five double amino acid change SNPs were found to potentially affect the structure and function of the MSH2 protein. Additionally, twelve identified SNPs were linked to specific types of cancer, such as hereditary nonpolyposis, colorectal cancer, and Lynch syndrome.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Review
Oncology
Harleen Kaur Walia, Parul Sharma, Navneet Singh, Siddharth Sharma
Summary: Despite being in the nascent stage, both prognosis and treatment for SCLC patients have made advancements with the introduction of immunotherapy as the fourth pillar of treatment. T cell checkpoint inhibitors show potential in anti-cancer activity and prolonging survival in SCLC patients by enhancing immune response. Immunotherapeutic approaches, such as TLR9 agonist and anti-Notch signaling, have also shown promise in clinical trials for SCLC.
CURRENT TREATMENT OPTIONS IN ONCOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Divyanshu Aggarwal, Vinod Kumar, Siddharth Sharma
Summary: This review discusses the importance of drug-eluting implants in promoting bone healing and the research on different drugs in combating bacterial adhesion. Attention has shifted towards biodegradable materials due to the drawbacks of permanent implants.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Pharmacology & Pharmacy
Harleen Walia, Parul Sharma, Navneet Singh, Siddharth Sharma
Summary: This study investigated the associations between SNPs of Phase II detoxifying genes and platinum-induced toxicity in lung cancer patients. The findings showed that the NQO1 609 C > T polymorphism was associated with reduced risk of constipation and anorexia. The SULT1A1 Arg(213)His polymorphism was not associated with increased risk of nephrotoxicity. The NAT2 polymorphism exhibited increased risk of hematological toxicity.
Article
Gastroenterology & Hepatology
Parul Sharma, Siddharth Sharma
Summary: This study used computational tools to predict the association between ABCC2 gene variants and Dubin-Johnson syndrome (DJS). The study found that 41 ABCC2 gene variants may be important etiological candidates for DJS in humans, and these variants had damaging effects on the function of the MRP2 protein, potentially leading to deficient bilirubin transportation capacity.
ARAB JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anmol Bhatia, Atul Kumar Upadhyay, Siddharth Sharma
Summary: This article introduces the role of noncoding RNAs, such as miRNAs, in cancer treatment, as well as the research progress in combining miRNAs with anticancer drugs. It has been found that miRNAs play critical regulatory roles in key signaling cascades related to cancer cell proliferation, apoptosis, migration, and invasion. Combining miRNAs with anticancer drugs can effectively treat various types of cancer and improve the efficacy of treatment.
Article
Biochemistry & Molecular Biology
Sidhartha Singh, Navneet Singh, Siddharth Sharma
Summary: The present study found a relationship between polymorphisms in MLH1, MSH2, MSH3, and MSH6 genes and toxicity in North Indian lung cancer patients receiving platinum-based doublet chemotherapy. Certain gene polymorphisms were associated with an increased risk of anemia, leukopenia, neutropenia, and gastrointestinal toxicity. These polymorphisms could potentially serve as predictors for pretreatment evaluation in lung cancer patients.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2022)
Article
Pharmacology & Pharmacy
Parul Sharma, Navneet Singh, Siddharth Sharma
Summary: The study found that solute carrier gene polymorphisms affect the survival rate of lung cancer patients in Northern India, as well as the incidence of adverse events and toxicity in patients receiving platinum-based doublet chemotherapy.
JOURNAL OF CLINICAL PHARMACY AND THERAPEUTICS
(2022)
Article
Environmental Sciences
Harleen Kaur Walia, Parul Sharma, Navneet Singh, Siddharth Sharma
Summary: Lung cancer is a multifactorial carcinoma with diverse heterogeneity. Genetic variations in drug-metabolizing enzymes may lead to defective detoxification and clearance of carcinogenic compounds. Certain genetic combinations of Phase II detoxification genes can have a protective effect against lung cancer risk, while others can increase the risk.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Parul Sharma, Navneet Singh, Siddharth Sharma
Summary: This study assessed the impact of ABC transporter gene variants on the prognosis and clinical outcomes of lung cancer patients. The results showed that certain gene variants were associated with poor survival outcomes, suggesting that genotype testing is essential for predicting prognosis in lung cancer patients.
JOURNAL OF GENE MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Parul Sharma, Navneet Singh, Siddharth Sharma
Summary: This study investigates the relationship between genetic variations in ABCB1, ABCC1, ABCC2, and ABCG2 genes and the adverse events in lung cancer patients undergoing chemotherapy. The study found that certain gene polymorphisms are associated with leukopenia, anemia, diarrhea, and liver injury.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2023)
Article
Pharmacology & Pharmacy
Depanshi Pandit, Parul Sharma, Harsh Yadav, Kranti Garg, Vishal Chopra, Siddharth Sharma
Summary: This study reveals the important role of IL-4 in the immune response to allergen exposure. The IL4RAQ576R gene polymorphism is significantly associated with COPD susceptibility, and it has a protective effect on GOLD stage four patients.
Article
Physiology
Harsh Yadav, Deepanshi Pandit, Sidhartha Singh, Parul Sharma, Kranti Garg, Nidhi Girdhar, Karan Sharma, Vishal Chopra, Siddharth Chopra, Siddharth Sharma
Summary: This study assessed the relationship between GSTT1 and GSTM1 gene polymorphism and COPD susceptibility and clinical parameters. The results showed a significant correlation between the GSTT1(-) genotype and COPD development, while no association was observed in the GSTM1(-) genotype. Female individuals with the GSTT1(-) genotype were more vulnerable to COPD.
EXPERIMENTAL PHYSIOLOGY
(2023)