D-galactose induced inflammation lipid peroxidation and platelet activation in rats

Background: To investigate events possibly related to the development of D-galactose induced senescence, we examined whether 8-iso PGF(2 alpha), formation, a marker of in vivo lipid peroxidation is altered and whether its biosynthesis is associated with 11-dehydro-TXB2 excretion rate, as a marker of in vivo platelet activation. In this setting, we also investigated the relationship between proinflammatory mediators (IL-6 and TNF-alpha from one, and lipid peroxidation and platelet activation, from another aspect. Methods and results: Forty animals were divided, depending on treatment with D-galactose into: placebo and D-galactose treated rats. 8-iso-PGF(2 alpha), IL-6 and TNF-alpha were measured in plasma, while 11-dehydro-TXB2 was determined in the urine after a six week treatment with D-galactose. Compared to placebo, D-galactose treated animals showed significantly higher levels of all measured parameters. Conclusions: D-galactose induced changes in the rate of F-2-isoprostane formation are associated with the changes in the excretion rate of 11-dehydro-TXB2. (C) 2014 Elsevier Ltd. All rights reserved.