Article
Biochemistry & Molecular Biology
Kai Halwas, Lennard-Maximilian Doering, Franziska Valentina Oehlert, R. Juergen Dohmen
Summary: Polyamines are important biogenic polycations that play essential roles in various cellular processes and diseases. The biosynthesis of polyamines involves the conversion of ornithine to putrescine by ornithine decarboxylase (ODC). The regulation of ODC by ODC antizyme (OAZ) involves a unique mechanism of posttranslational regulation through polyamine-regulated bypassing of an internal translation termination codon. The depletion of polyamines inhibits translation across the OAZ1 ribosomal frameshift (RFS) site by affecting eIF5A hypusination and RFS directly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Heidi Kaprio, Arafat Siddiqui, Lotta Saustila, Vanina D. Heuser, Maria Gardberg
Summary: The EWSR1::CREM fusion gene, caused by a chromosomal translocation t(10;22)(p11;q12), has been found in various types of cancers with different malignancy levels. The fusion gene leads to production of a chimeric protein, EWSR1::CREM. However, the molecular mechanisms of EWSR1::CREM protein's oncogenic properties have not been systematically characterized. This study used siRNA mediated knockdown to investigate the effects of depletion of EWSR1::CREM protein on gene expression in a melanoma cell line, identified altered pathways and demonstrated the functional differences. The findings contribute to understanding the pathogenesis of tumors with EWSR1::CREM fusion gene and may aid in the development of novel therapeutic strategies.
SCIENTIFIC REPORTS
(2023)
Article
Cardiac & Cardiovascular Systems
Weiwei An, Le A. Luong, Neil P. Bowden, Mei Yang, Wei Wu, Xinmiao Zhou, Chenxin Liu, Kaiyuan Niu, Jun Luo, Cheng Zhang, Xiaolei Sun, Robin Poston, Li Zhang, Paul C. Evans, Qingzhong Xiao
Summary: Cezanne plays a key role in VSMC proliferation and migration in pathological arterial remodelling, and it is highly expressed in human atherosclerotic lesions.
CARDIOVASCULAR RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Lili Zhang, Sihao Qu, Lu Wang, Chunguo Wang, Qinghe Yu, Zhimin Zhang, Yirui Diao, Binbin Zhang, Yadong Li, Yuanyuan Shi, Peng Wang
Summary: TL treatment in BLM-induced mice significantly reduced collagen deposition in lung tissues. TL also inhibited the protein expression of alpha-SMA and mRNA expressions of Col1a1 and Fn1. Using TMT technology, we identified 253 differentially expressed proteins related to PPI networks and various KEGG pathways, with the arginase-ornithine pathway being highly significant.
FRONTIERS IN PHARMACOLOGY
(2021)
Editorial Material
Dermatology
Kira Allmeroth, Martin S. Denzel
Summary: Polyamines play a critical role in keratinocyte differentiation, with AMD1 mediating the shift in polyamine ratios. Targeting polyamine availability may be beneficial in treating hyperproliferative skin disorders.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Chemistry, Analytical
Yuze Li, Lixia Jiang, Zhenpeng Wang, Yiran Wang, Xiaohua Cao, Lingwei Meng, Jinghan Fan, Caiqiao Xiong, Zongxiu Nie
Summary: A noninvasive profiling method for bladder cancer diagnosis based on carbonyl metabolic fingerprints using a DSI-MS platform with DMED as a labeling reagent has been developed. This method achieves a high accuracy in distinguishing bladder cancer patients from controls. It may pave the way for patient-friendly point-of-care diagnosis of bladder cancer.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yue Sun, Zhi Zhou, Bing Xie, Zhonghua Wang, Zeper Abliz
Summary: By establishing an AFADESI-MSI system, the molecular information of zebrafish was fully characterized, providing high-quality visual distribution information for over 1000 functional metabolites and mapping a global metabolic network. This comprehensive and in-depth molecular information facilitates the use of zebrafish models in understanding human diseases and developing zebrafish disease models.
ANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Cun-Cui Kong, Xin Wei, Guang-Lei Liu, Zhen-Ming Chi, Zhe Chi
Summary: In this study, the strain Aureobasidium melanogenum HN6.2 was genetically engineered to enhance siderophore production, ornithine synthesis, and putrescine production. The overexpression of specific genes successfully increased the yields of ornithine and putrescine, making this strain a promising candidate for industrial applications.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Oncology
Caleb C. Capellen, Jose Ortega-Rodas, M. Jane Morwitzer, Hadassha M. N. Tofilau, Matthew Dunworth, Robert A. Casero, Surabhi Chandra
Summary: Diabetes can accelerate the progression of certain cancer subtypes, including breast cancer, through the modulation of cellular polyamines. Polyamine inhibitors show promise in suppressing cancer cell proliferation under high glucose conditions, pointing towards a potential therapeutic target for diabetic breast cancer patients.
BREAST CANCER RESEARCH AND TREATMENT
(2021)
Article
Multidisciplinary Sciences
Min-Sik Lee, Courtney Dennis, Insia Naqvi, Lucas Dailey, Alireza Lorzadeh, George Ye, Tamara Zaytouni, Ashley Adler, Daniel S. Hitchcock, Lin Lin, Megan T. Hoffman, Aladdin M. Bhuiyan, Jaimie L. Barth, Miranda E. Machacek, Mari Mino-Kenudson, Stephanie K. Dougan, Unmesh Jadhav, Clary B. Clish, Nada Y. Kalaany
Summary: This study reveals the distinct dependence of pancreatic ductal adenocarcinoma (PDA) on de novo ornithine synthesis from glutamine, mediated by ornithine aminotransferase (OAT), which is required for tumor growth and polyamine synthesis. The reliance on OAT-mediated ornithine synthesis in PDA provides a potential therapeutic window for treating pancreatic cancer with minimal toxicity.
Article
Biotechnology & Applied Microbiology
Hari Priya Sivakumar, Sathish Sundararajan, Venkatesh Rajendran, Sathishkumar Ramalingam
Summary: This study identified the distribution of plant ODC and revealed the highly conserved regulatory motifs among diverse plant species. ODC plays an important role in plant metabolic pathways and is crucial for the development of ultra-low nicotine content tobacco lines.
Article
Reproductive Biology
Christopher L. J. Lavergne, Yong Tao, Yanping Ren, Nahantara Lafleur, X. Johne Liu
Summary: In mammalian species, the ovaries produce ODC and putrescine during ovulation. Aged mice have reduced levels of periovulatory ODC and putrescine. Supplementation of putrescine improves fertility in aged mice. However, increasing putrescine levels may lead to unwanted side effects. Attempts to increase ovarian putrescine levels through L-ornithine supplementation did not yield desired results.
BIOLOGY OF REPRODUCTION
(2022)
Article
Biology
Surender Rajasekaran, Caleb P. Bupp, Mara Leimanis-Laurens, Ankit Shukla, Christopher Russell, Joseph Junewick, Emily Gleason, Elizabeth A. VanSickle, Yvonne Edgerly, Bryan M. Wittmann, Jeremy W. Prokop, Andre S. Bachmann
Summary: This study highlights the therapeutic strategy of repurposing drugs for the treatment of rare diseases, as evidenced by the successful use of eflornithine to normalize polyamine levels and improve neurological symptoms and cortical architecture in a patient with a rare syndrome caused by ODC gain-of-function variants.
Article
Biology
Martin Weber, Rolf Schreckenberg, Klaus-Dieter Schlueter
Summary: Elevated levels of uric acid can stress cardiomyocytes and may contribute to heart failure.
Article
Food Science & Technology
Zhe Xu, Jiale Chang, Jiamin Zhou, Yixin Shi, Hui Chen, Lingyu Han, Maolin Tu, Tingting Li
Summary: This study found that the ornithine decarboxylase gene (ODC) is involved in putrescine biosynthesis and successfully expressed it in E. coli. Functional analysis confirmed that the ODC protein can catalyze the decarboxylation of ornithine to putrescine. Tea polyphenols were identified as effective inhibitors for controlling putrescine content in pickled fish.
Article
Biochemistry & Molecular Biology
Emma Anders, Sara Dahl, Daniel Svensson, Bengt-Olof Nilsson
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Emma Anders, Daniel Nebel, Johannes Westman, Heiko Herwald, Bengt-Olof Nilsson, Daniel Svensson
BIOCHEMICAL JOURNAL
(2018)
Review
Biochemistry & Molecular Biology
Bengt-Olof Nilsson, Lo Persson
BIOCHEMICAL SOCIETY TRANSACTIONS
(2019)
Article
Cardiac & Cardiovascular Systems
Amalia Forte, Xiaoke Yin, Marika Fava, Ciro Bancone, Marilena Cipollaro, Marisa De Feo, Manuel Mayr, Marjan Jahangiri, Alessandro Della Corte
EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
(2019)
Review
Medicine, Research & Experimental
Amalia Forte, Marilena Cipollaro, Marisa De Feo, Alessandro Della Corte
Review
Cardiac & Cardiovascular Systems
Amalia Forte, Carmela Rita Balistreri, Marisa De Feo, Alessandro Della Corte, Per Hellstrand, Lo Persson, Bengt-Olof Nilsson
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2019)
Article
Dentistry, Oral Surgery & Medicine
Alexandra Aidoukovitch, Emma Anders, Sara Dahl, Daniel Nebel, Daniel Svensson, Bengt-Olof Nilsson
JOURNAL OF PERIODONTAL RESEARCH
(2019)
Article
Dentistry, Oral Surgery & Medicine
Alexandra Aidoukovitch, Sara Dahl, Felicia Faelt, Daniel Nebel, Daniel Svensson, Ellen Tufvesson, Bengt-Olof Nilsson
EUROPEAN JOURNAL OF ORAL SCIENCES
(2020)
Article
Cell Biology
Sara Dahl, Samuel Cerps, Catarina Rippe, Karl Sward, Lena Uller, Daniel Svensson, Bengt-Olof Nilsson
INFLAMMATION RESEARCH
(2020)
Correction
Multidisciplinary Sciences
Francesca Cuomo, Antonietta Coppola, Chiara Botti, Ciro Maione, Amalia Forte, Lucia Scisciola, Giuseppina Liguori, Ilaria Caiafa, Matilde Valeria Ursini, Umberto Galderisi, Marilena Cipollaro, Lucia Altucci, Gilda Cobellis
SCIENTIFIC REPORTS
(2020)
Review
Biochemistry & Molecular Biology
Bengt-Olof Nilsson
Review
Dentistry, Oral Surgery & Medicine
Bengt-Olof Nilsson
Summary: PDL cells may serve as local immune cells in periodontal tissues, producing pro-inflammatory cytokines in response to various stimuli including bacterial lipopolysaccharides and mechanical stress. Modulators like dexamethasone, vitamin D, and LL-37 have been shown to reduce the production of pro-inflammatory cytokines by PDL cells, suggesting their potential role in regulating oral innate immunity and periodontitis.
JOURNAL OF PERIODONTAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Sara Bodahl, Samuel Cerps, Lena Uller, Bengt-Olof Nilsson
Summary: LL-37 enhances poly I:C-induced expression of TLR3 mRNA and protein, possibly through enhanced import of poly I:C. Dexamethasone reduces LL-37/poly I:C-induced TLR3 expression by attenuating NF-kappa B activity.
Article
Dentistry, Oral Surgery & Medicine
Alexandra Aidoukovitch, Sara Bodahl, Ellen Tufvesson, Bengt-Olof Nilsson
Summary: This study found that desquamated oral epithelial cells (DOECs) express epidermal growth factor (EGF), suggesting they contribute to salivary EGF levels and may play a role in gingival epithelial repair and wound healing.
INTERNATIONAL JOURNAL OF DENTISTRY
(2022)
Article
Biochemistry & Molecular Biology
Elisabeth Bankell, Xiaoyan Liu, Martin Lundqvist, Daniel Svensson, Karl Sward, Emma Sparr, Bengt-Olof Nilsson
Summary: LL-37 is rapidly internalized by MG63 cells and accumulates in mitochondria. It triggers the release of pro-apoptotic AIF and directly affects mitochondrial membrane structural properties.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)