Article
Biochemical Research Methods
Mengle Wang, Kathrin Schuster, Stefan Asam, Michael Rychlik
Summary: Previous methods for B12 analysis involved cyanidation conversion to determine total B12 concentration. However, this study revealed limitations associated with incomplete conversions of cobalamins during the cyanidation process. LC-MS/MS was used to detect unconverted cobalamins, but further development of a reliable monitoring method and improvement in instrument sensitivity and stability are needed to overcome the current limitations.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Raymond Neil Dalton, Timothy Scott Isbell, Ryan Ferguson, Louis Fiore, Andrei Malic, Jeffrey Anton DuBois
Summary: Early detection of CKD using point of care creatinine and eGFR testing improves patient management outcomes. A study was conducted to evaluate the use of a whole blood creatinine/eGFR device in a rural Nicaraguan population. Results showed a good correlation between all creatinine methods and the gold standard ID-LCMS method.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Jianyi Liu, Wen Zhu, Haofeng Sun, Dewei Song, Peng Xiao, Bei Xu, Hongmei Li
Summary: The development of a certified reference material (CRM) for natural CRP, named GBW09228, was reported, with certification results traceable to SI units. This CRM can be used to calibrate and verify the accuracy of CRP measurements, providing a foundation for establishing a complete traceability chain for CRP.
ANALYTICAL METHODS
(2021)
Article
Biochemistry & Molecular Biology
Makoto Inoue, Hideaki Suzuki, Kohji Meno, Shan Liu, Tatsumi Korenaga, Kazuhiko Uchida
Summary: The study developed a blood test based on LC-MS/MS to detect plasma proteins that can be used to identify mild cognitive impairment and Alzheimer's disease. They identified eight plasma protein biomarkers that can differentiate between MCI and AD. The clinical potential of these biomarkers was evaluated using ROC analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Iris Fiby, Marta Magdalena Sopel, Herbert Michlmayr, Gerhard Adam, Franz Berthiller
Summary: A new LC-MS/MS method for determining Fusarium mycotoxin in maize was developed and validated, with the use of stable-isotope dilution technique in a complex cereal matrix. The method successfully quantified the toxins and efficiently corrected matrix effects in the validation study.
Article
Chemistry, Analytical
Christine E. Mathew, Vinai K. Rai, Chelsea Ottenfeld, Russ Keller
Summary: Isotope dilution mass spectrometry using traceable standards as spikes combined with isotope ratio measurements by thermal ionization or inductively coupled plasma mass spectrometer instruments is the preferred methodology for element content determinations and isotopic composition measurements in various applications, especially those involving highly radioactive isotopes like the actinide elements. This technique takes advantage of modern mass spectrometry instrumentation sensitivity to minimize material usage and enables automated measurement sequences with minimal analyst intervention.
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Xianjiang Li, Wen Ma, Qinghe Zhang, Hongmei Li, Huwei Liu
Summary: A simple and reliable extraction method for the determination of patulin in apple juice has been developed using amine-functionalized polymeric solid-phase extraction cartridges. Isotope dilution liquid chromatography tandem mass spectrometry was utilized to minimize matrix effects and ensure reliable quantification. Results showed negligible matrix effects and satisfactory sensitivity, providing a promising method for routine quality control in patulin detection.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Biochemical Research Methods
Mogjiborahman Salek, Jonas D. Foerster, Wolf-Dieter Lehmann, Angelika B. Riemer
Summary: In mass spectrometry-based proteomics, heavy internal standards are used for validating target peptide detections and calibrating peptide quantitation. However, contamination in heavy labelled synthetic peptides may compromise the detection and quantitation of low abundant cellular peptides. It is important to establish guidelines to prevent false-positive identifications of endogenous light peptides and errors in their quantitation from biological samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Nutrition & Dietetics
Lisa Obermaier, Barbara Paes Miglioli da Mata, Caio Humberto Perego, Katia Sivieri, Mateus Kawata Salgaco, Andre Gonzaga dos Santos, Ruth Boehni, Viola Groehn, Jean-Pierre Knapp, Michael Rychlik
Summary: Brazil has a diverse plant community with underutilized non-conventional food crops that could contribute to food security. A study on Brazilian non-conventional fruits and leaves revealed them as an important source of folates, with Pequi having the highest folate content among the fruits studied. The study also highlighted the variability in folate content among different cultivars and growing locations.
FRONTIERS IN NUTRITION
(2023)
Article
Pharmacology & Pharmacy
Rossella Barone, Matteo Conti, Pier Giorgio Cojutti, Milo Gatti, Pierluigi Viale, Federico Pea
Summary: Dalbavancin (DBV) is a long-acting antistaphylococcal lypoglycopeptide increasingly used for long-term treatment of subacute and/or chronic infections. Population pharmacokinetic studies have shown that two 1500mg doses of DBV 1 week apart can effectively treat infections for weeks. This study developed and validated a fast and simple analytical method for measuring DBV concentrations in human plasma microsamples using Liquid Chromatography-Isotope Dilution Tandem Mass Spectrometry (ITD LC-MS/MS) technique.
Article
Biochemical Research Methods
Claudia Gaither, Robert Popp, Sophia P. Borchers, Kjartan Skarphedinsson, Finnur F. Eiriksson, Margret Thorsteinsdottir, Yassene Mohammed, Christoph H. Borchers
Summary: The study demonstrated that leaving a lyophilized peptide mixture at room temperature for up to 20 days does not affect the quantitative performance of mass spectrometry analysis, making it a feasible option for sample transportation. Most peptide concentrations showed minimal fluctuations across different time points, indicating the stability of the peptide mixture under the tested conditions.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Multidisciplinary Sciences
Christopher Hodgkins, Laura K. Buckton, Gregory J. Walker, Ben Crossett, Stuart J. Cordwell, Andrea R. Horvath, William D. Rawlinson
Summary: Diagnosis of SARS-CoV-2 infection has primarily relied on RT-PCR and serology, but LC-MS/MS has the potential to provide supplemental data about the presence of viral proteins and improve the diagnosis of viral infection. Researchers developed a proteomics workflow using serum-free culture conditions and successfully detected target virus peptides in clinical samples using LC-MS/MS. This method is an important step towards the use of LC-MS/MS in viral infection diagnosis.
SCIENTIFIC REPORTS
(2022)
Article
Medical Laboratory Technology
Ming-Hui Yang, Shih-Cheng Chen, Ko-Chin Chen, Han-Ping You, Hsin-Yi Wu, Yi-Ming Arthur Chen, Ying-Fong Huang, Ming-Yii Huang, Cheng-Hui Yuan, Po-Chiao Lin, Yu-Chang Tyan
Summary: The study introduced a method to quantify progesterone concentration in human serum based on isotope dilution mass spectrometry. By extracting and cleaning serum samples, combined with mass spectrometry, progesterone concentration could be accurately determined. Results demonstrated that this method showed good robustness and consistency in measuring progesterone levels.
CLINICA CHIMICA ACTA
(2021)
Article
Chemistry, Applied
Wei Xia, Xiangjun Fang, Yuan Gao, Weijie Wu, Yanchao Han, Ruiling Liu, Hailong Yang, Hangjun Chen, Haiyan Gao
Summary: Food quality, safety, and nutrient metabolism play a key role in human health. However, contaminants and metabolic dysregulation in food can be problematic. Chromatography-mass spectrometry technology, especially when combined with stable isotopes, is widely used in food analysis and nutrient metabolism research. This study provides an overview of stable isotope technology applications in quantifying contaminant residues and nutrient metabolism, aiming to contribute to effective analysis techniques for protecting food quality and human health.
Article
Chemistry, Analytical
Honglin Lin, Lei Ni, Huafeng Chen, Weisheng Xu
Summary: A new sample pretreatment technique combining cold-induced liquid-liquid microextraction with serially-coupled columns for SIDA-UHPLC-MS/MS has been developed to analyze ultra-trace levels of Alternaria toxins in complex samples. The method achieves simultaneous purification and enrichment, with a high limit of quantitation and without the need for additional sample manipulation. It has shown good linearity, accuracy, and precision in validation experiments, and successfully applied to analyze samples from the Mediterranean region.
ANALYTICA CHIMICA ACTA
(2022)
Article
Virology
Xiaofang Xu, Dianhua Qiao, Morgan Mann, Roberto P. Garofalo, Allan R. Brasier
Article
Virology
Morgan Mann, David S. Roberts, Yanlong Zhu, Yi Li, Jia Zhou, Ying Ge, Allan R. Brasier
Summary: The study reveals that RSV infection alters the interaction proteins of BRD4, with 101 proteins significantly enriched in the BRD4 complex responsive to both RSV infection and BRD4 inhibition. These proteins are mainly transcription factors and transcriptional coactivators, including members of the AP1 transcription factor complex.
Article
Virology
Xiaofang Xu, Morgan Mann, Dianhua Qiao, Allan R. Brasier
Summary: The research utilized SMRT sequencing and short-read mRNA sequencing to characterize changes in mRNA processing in epithelial cells in response to RSV infection, revealing differential splicing and polyadenylation, particularly involving cell cycle control and the innate immune response pathway.
Article
Physiology
Dianhua Qiao, Melissa Skibba, Xiaofang Xu, Roberto P. Garofalo, Yingxin Zhao, Allan R. Brasier
Summary: Paramyxoviridae, respiratory syncytial virus (RSV), and murine respirovirus are enveloped, negative-sense RNA viruses that cause vertebrate lower respiratory tract infections (LRTIs). RSV infection induces the activation of the hexosamine biosynthetic pathway (HBP), as well as mesenchymal transition (EMT) and ECM remodeling through the IRE1 alpha-XBP1 arm of the unfolded protein response (UPR). Murine respirovirus induces similar effects in a host-dependent manner, suggesting a role for the IRE1 alpha-XBP1s arm in paramyxovirus-induced metabolic adaptation and mucosal remodeling.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
David S. Roberts, Morgan Mann, Jake A. Melby, Eli J. Larson, Yanlong Zhu, Allan R. Brasier, Song Jin, Ying Ge
Summary: Researchers have successfully elucidated the complete structure of newly discovered O-glycans on the S protein regional-binding domain (SR-BD) of the SARS-CoV-2 virus through a hybrid mass spectrometry approach. This method can provide insights into the interactions between the virus and host cells, laying a foundation for understanding the functional roles of O-glycans in SARS-CoV-2 and other glycoproteins.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Gastroenterology & Hepatology
Yi Li, Jianping Chen, Andrew A. Bolinger, Haiying Chen, Zhiqing Liu, Yingzi Cong, Allan R. Brasier, Irina Pinchuk, Bing Tian, Jia Zhou
Summary: Inflammatory bowel disease (IBD) is a serious chronic gastrointestinal disease, with long-term inflammation contributing to neoplastic transformation and colorectal cancer development. The discovery of new small molecule drugs targeting specific pathways in mucosal inflammation offers hope for the treatment of IBD.
INFLAMMATORY BOWEL DISEASES
(2021)
Article
Biochemical Research Methods
Yanlong Zhu, Stephane Esnault, Ying Ge, Nizar N. Jarjour, Allan R. Brasier
Summary: Airway remodeling in patients with asthma is associated with leukocyte numbers and remodeling markers. Patients with lower FEV1 have distinct dynamic responses to allergens.
CLINICAL PROTEOMICS
(2022)
Article
Biochemistry & Molecular Biology
Yanlong Zhu, Stephane Esnault, Ying Ge, Nizar N. Jarjour, Allan R. Brasier
Summary: This study analyzed metabolites in bronchoalveolar fluid (BALF) during a bronchial antigen challenge and identified distinct metabolic responses, providing insight into pathogenic and protective subtypes in allergic asthma.
Editorial Material
Virology
Allan R. Brasier
Article
Biochemistry & Molecular Biology
Dianhua Qiao, Melissa Skibba, Xiaofang Xu, Allan R. Brasier
Summary: Epithelial mesenchymal plasticity (EMP) is a complex cellular reprogramming event that plays a major role in tissue homeostasis. Recently, the unfolded protein response (UPR) was found to trigger EMP through the IRE1 alpha-XBP1s axis, enhancing glucose shunting to protein N glycosylation. By identifying the genomic targets of XBP1s using CUT&RUN, it was discovered that XBP1s peaks were located near various promoters of genes controlling Rho-GTPase signaling, N-linked glycosylation, and ER-Golgi transport. Moreover, the IRE1 alpha-XBP1s pathway was shown to regulate mesenchymal transcription factors and hexosamine biosynthesis in EMP by recruiting activated RNA Pol II to GC-rich promoters.
NUCLEIC ACIDS RESEARCH
(2023)
Editorial Material
Medicine, Research & Experimental
Allan R. Brasier
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Review
Medicine, Research & Experimental
Allan. R. R. Brasier, Shannon. L. L. Casey, Peggy Hatfield, Patrick. W. W. Kelly, Whitney. A. A. Sweeney, Marin Schweizer, Bo Liu, Elizabeth. S. S. Burnside
Summary: This study proposes a model for developing leadership in translational teams (TTs) from team formation to dissemination and implementation. It identifies the stable impact of leadership behaviors on team development and emphasizes the importance of ongoing reflection, evaluation, and practice to enhance leadership skills. The study also provides a comprehensive multi-level evaluation framework to track the growth of TT leadership skills.
JOURNAL OF CLINICAL AND TRANSLATIONAL SCIENCE
(2023)
Review
Medicine, Research & Experimental
Allan R. Brasier, Shannon L. Casey, Felice Resnik, Betsy Rolland, Elizabeth S. Burnside
Summary: Successful translation involves the application of research and product development to advance healthcare interventions. Training in team skills and attitudes can enhance translational team performance. Interdisciplinary teams undergo developmental phases, with each phase promoting knowledge generation and translation. Antecedents of stage-dependent competencies and assessment rubrics can guide training interventions for improving performance in the clinical context.
JOURNAL OF CLINICAL AND TRANSLATIONAL SCIENCE
(2023)
Review
Medicine, Research & Experimental
Allan R. Brasier, Elizabeth S. Burnside, Betsy Rolland
Summary: A translational team is a type of interdisciplinary team that aims to improve human health. Understanding how to promote team performance in the areas of affect, communication, management, collaborative problem-solving, and leadership is essential. This study conducted a literature review to identify key competencies that enhance team performance and develop strategies for training interventions.
JOURNAL OF CLINICAL AND TRANSLATIONAL SCIENCE
(2023)
Article
Behavioral Sciences
Suresh K. Bhavnani, Shyam Visweswaran, Rohit Divekar, Allan R. Brasier
JOURNAL OF APPLIED BEHAVIORAL SCIENCE
(2019)
