Article
Oncology
Joana Albuquerque, Diana Neto da Silva, Teresa Padrao, Luisa Leal-Costa, Rita Bizarro, Jorge Correia, Carlota Baptista, Madalena Machete, Gil Prazeres, Ines Margarido, Goncalo Fernandes, Pedro Simoes, Teresa Timoteo, Fabio Lopes, Joao Godinho, Joao Moreira-Pinto, Tania Rodrigues, Ana Faria, Catarina Pulido, Luis Cirnes, Jose A. Teixeira, Jose L. Passos Coelho
Summary: Liquid biopsy can be a valuable tool to monitor RAS mutation loss in the treatment of metastatic colorectal cancer (mCRC). This study assessed the prognostic value of RAS mutation loss in liquid biopsy and found that it could provide an additional treatment line for patients with NeoRAS-wt in advanced mCRC.
Article
Oncology
Laura E. Fischer, Sebastian Stintzing, Volker Heinemann, Ulrich Keilholz, Dietmar Keune, Claudia Vollbrecht, Thomas Burmeister, Andreas Kind, Lena Weiss, David Horst, Thomas Kirchner, Frederick Klauschen, Andreas Jung, Christoph Benedikt Westphalen, Ivan Jelas
Summary: This study retrospectively analyzed the implementation of liquid biopsies (LB) in the clinical management of colorectal cancer (CRC) patients between 2017 and 2021. The results indicate that the potential of LB as a diagnostic tool to drive personalized treatment in CRC patients is not yet fully exploited in everyday clinical practice.
FRONTIERS IN ONCOLOGY
(2022)
Review
Oncology
Marcel Kemper, Carolin Krekeler, Kerstin Menck, Georg Lenz, Georg Evers, Arik Bernard Schulze, Annalen Bleckmann
Summary: Liquid biopsy is a novel method in cancer diagnostics that is less invasive for patients compared to conventional tissue biopsies. This review provides an overview of lung cancer biomarkers identified in liquid biopsy assays and discusses the differences, current applications, and future perspectives of liquid biopsies in lung cancer.
Review
Oncology
Gianluca Mauri, Pietro Paolo Vitiello, Alberto Sogari, Giovanni Crisafulli, Andrea Sartore-Bianchi, Silvia Marsoni, Salvatore Siena, Alberto Bardelli
Summary: Colorectal cancer is a prevalent and deadly cancer, and there is a clinical need for better patient selection for systemic treatments. Genotyping circulating tumour DNA is a potential method to personalize treatment and overcome limitations of traditional biopsy approaches. This review summarizes the evidence of ctDNA applicability in colorectal cancer and discusses ongoing clinical trials.
BRITISH JOURNAL OF CANCER
(2022)
Article
Oncology
Hector Martinez-Gregorio, Clara Diaz-Velasquez, Mario Esau Romero-Pina, Miguel Ruiz de la Cruz, Norma Laura Delgado-Buenrostro, Aldo De la Cruz-Montoya, Yolanda Irasema Chirino, Luis Ignacio Terrazas, Luis Alberto Medina, Felipe Vaca-Paniagua
Summary: This study aims to demonstrate the feasibility of using circulating free DNA (cfDNA) as a liquid biopsy for early detection of colorectal cancer (CRC). The researchers successfully detected mutations in liquid biopsies of early stages of tumor development, providing evidence for the potential use of this method.
Review
Medicine, Research & Experimental
Siobhan Connal, James M. Cameron, Alexandra Sala, Paul M. M. Brennan, David S. S. Palmer, Joshua D. D. Palmer, Haley Perlow, Matthew J. J. Baker
Summary: Cancer is a global pandemic, causing significant burdens on individuals, families, and healthcare systems. Lack of screening programs and compliance challenges contribute to the high death rate of cancer worldwide. Liquid biopsy strategies for early detection of cancer are not sensitive enough, and early detection is crucial for better outcomes. This review examines the current market of liquid biopsy and explores the potential of combining spectroscopic and -omic methodologies for more efficient cancer diagnostics.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Review
Biochemical Research Methods
Chi-Ju Kim, Liang Dong, Sarah R. Amend, Yoon-Kyoung Cho, Kenneth J. Pienta
Summary: Liquid biopsy serves as a valuable tool in cancer diagnosis and treatment guidance, utilizing biomarkers such as CTCs, EVs, and ctDNA. Prostate cancer ranks as the second most common cancer in males globally, with management strategies focusing on biopsy and prevention of metastatic disease.
Review
Oncology
Diamantis Tsilimigras, Ioannis Ntanasis-Stathopoulos, Timothy M. Pawlik
Summary: Liquid biopsies have gained attention in the field of oncologic surgery and can improve prognostication and monitor disease evolution. Although not currently standard of care, liquid biopsies have shown promising results in the treatment of CRLM patients.
Article
Cell Biology
Valentina Magri, Luca Marino, Chiara Nicolazzo, Angela Gradilone, Gianluigi De Renzi, Michela De Meo, Orietta Gandini, Arianna Sabatini, Daniele Santini, Enrico Cortesi, Paola Gazzaniga
Summary: This study aimed to evaluate the clinical value of circulating tumor cell (CTC) dynamics in metastatic colorectal cancer (mCRC) patients receiving first-line treatments. The CTC data from 218 patients were analyzed and four prognostic trajectories were identified. The study confirmed the clinical significance of CTC positivity and suggested that CTC trajectories are better prognostic indicators than CTC enumeration at baseline, providing potential biomarkers to monitor first-line treatments.
Review
Oncology
Andreas Koulouris, Christos Tsagkaris, Ippokratis Messaritakis, Nikolaos Gouvas, Maria Sfakianaki, Maria Trypaki, Vasiliki Spyrou, Manousos Christodoulakis, Elias Athanasakis, Evangelos Xynos, Maria Tzardi, Dimitrios Mavroudis, John Souglakos
Summary: CRC recurrence poses a major challenge to disease management, requiring urgent need for novel biomarkers. The interplay of microbiota in immune and inflammatory responses plays a crucial role in CRC development. Early detection of post-operative CRC recurrence is crucial, and ongoing trials on CTCs and ctDNA show promise as minimally invasive monitoring methods.
Review
Biochemistry & Molecular Biology
Sara Paczek, Marta Lukaszewicz-Zajac, Barbara Mroczko
Summary: Colorectal cancer is a common malignancy with heterogeneous characteristics. Despite increased awareness and expanded screening programs, there is still a need for new diagnostic tools for early detection. Classical tumor markers have limited effectiveness in CRC diagnosis, thus the search for new proteins involved in CRC is ongoing. Recent studies suggest the potential significance of granzymes, particularly Granzyme A, in CRC pathogenesis through their involvement in inflammation processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Cecilia Fernandez-Ponce, Noelia Geribaldi-Doldan, Ismael Sanchez-Gomar, Roberto Navarro Quiroz, Linda Atencio Ibarra, Lorena Gomez Escorcia, Ricardo Fernandez-Cisnal, Gustavo Aroca Martinez, Francisco Garcia-Cozar, Elkin Navarro Quiroz
Summary: The dysregulation of glycosyltransferase expression in colorectal cancer affects key molecules involved in oncogenesis and metastasis, impacting functions such as cell migration, cell-cell adhesion, apoptosis, and immune system regulation. Strategies to alter oligosaccharide processing and modify glycoconjugate structures have been evaluated to control CRC progression and prevent metastasis, while immunotherapy approaches target altered glycosylation as potential neo-antigens for tumor-specific T cells or engineered CAR T cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Sumeng Wang, Ke Zhang, Shanyue Tan, Junyi Xin, Qianyu Yuan, Huanhuan Xu, Xian Xu, Qi Liang, David C. Christiani, Meilin Wang, Lingxiang Liu, Mulong Du
Summary: Cancer is a major cause of death worldwide, prompting research into novel biomarkers for diagnosis. Circulating circRNAs, stably present in body fluids, exhibit distinct expression patterns that can be used to monitor cancer development and progression.
Article
Oncology
Elena Brozos-Vazquez, Ramon Manuel Lago-Leston, Marta Covela, Juan de la Camara Gomez, Ana Fernandez-Montes, Sonia Candamio, Yolanda Vidal, Francisca Vazquez, Alicia Abalo, Rosa Lopez, Cristina Blanco, Laura Muinelo-Romay, Isabel Ferreiros-Vidal, Rafael Lopez-Lopez
Summary: Evaluation of RAS mutations in plasma circulating tumour DNA (ctDNA) is an efficient approach for guiding therapeutic decisions in metastatic colorectal cancer (mCRC). However, discrepancies between liquid and tumour tissue biopsies can lead to misinterpretation of tumour genotype and compromise treatment efficacy. Our study identified clinicopathological profiles that influence plasmatic RAS mutation levels and detection, particularly in mCRC originating in the right colon and rectum. The absence of liver metastases and primary tumour resection were associated with reduced ctDNA levels and low agreement between tissue and ctDNA tests.
Review
Chemistry, Analytical
Faezeh Ghorbanizamani, Hichem Moulahoum, Emine Guler Celik, Figen Zihnioglu, Tutku Beduk, Tuncay Goksel, Kutsal Turhan, Suna Timur
Summary: Minimally invasive approaches in cancer diagnosis, such as liquid biopsies using blood samples, provide valuable information about the tumor and its state. However, isolating and detecting markers in complex matrices is challenging. Advances in polymer synthesis have led to enhanced platforms for liquid biopsies analysis, improving biosensor performance through increased sensitivity, improved binding, and avoidance of non-specific interactions. Additionally, polymer-based materials aid in signal amplification for low-concentration targets. This technology plays an essential role in oncology and clinical biomedicine, as shown by its critical application in liquid biopsies diagnosis.
Article
Oncology
Dan Hu, Ying Wang, Genxin Ji, Yu Liu
Summary: This study constructed prognostic models for advanced NPC patients treated with IMRT using machine learning algorithms. Random forest (RF) showed the best predictive performance and identified the most important predictors. RF was proposed as a simple and accurate tool for evaluating the prognosis of advanced NPC patients.
CURRENT PROBLEMS IN CANCER
(2024)
Article
Oncology
Leena Gupta, Pratibha Suku, Aishwarya Dash, Parveen Bose, Praveen Sharma, Nabhajit Mallik, Sreejesh Sreedharanunni, Neelam Varma, Aditya Jandial, Pankaj Malhotra, Man Updesh Singh Sachdeva
Summary: This study evaluated the load and characteristics of circulating tumor plasma cells (CTPCs) and circulating normal plasma cells (CNPCs) in newly diagnosed multiple myeloma patients using flow cytometry. Increased burden of CTPCs was associated with poor prognostic markers and poor response to therapy. CNPCs were lower in patients with thrombocytopenia and hypoalbuminemia.
CURRENT PROBLEMS IN CANCER
(2024)